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A. Harjuno Condro Haditomo
Department of Aquaculture, Fisheries and Marine Science Faculty, Diponegoro University, Tembalang, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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The Diversity of Causative Agent Associated With Bacterial Diseases on Catfish (Clarias gariepinus) with Molecular Based from Demak, Indonesia Sarjito Sarjito; A. Harjuno Condro Haditomo; Desrina Desrina; Restiana Ariyati; S. Budi Prayitno
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.723 KB) | DOI: 10.20884/1.oa.2018.14.2.553

Abstract

Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and  D14) that  were  caused  10–55% of fishes get sick  and  0–30%  fishes mortal.  On the other hand, there were 13 isolates do not cause both sick and mortality of fish.  On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.
Co-Authors - Desrina, - Restiana Ariyati S. Budi Prayitno Sarjito -