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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of Aquaculture Management and Technology Omni-Akuatika Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Litbang Provinsi Jawa Tengah
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Department Of Aquaculture, Fisheries And Marine Science Faculty, Diponegoro University, Tembalang, Semarang, Indonesia
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PENGARUH SALINITAS TERHADAP INFEKSI Infectious myonecrosis virus (IMNV) PADA UDANG VANAME Litopenaeus vannamei (Boone,1931) Umiliana, Mita; Sarjito, -; Desrina, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Udang vaname L. vannamei (Boone, 1931) merupakan salah satu produk perikanan yang diharapkan mampu menghasilkan devisa bagi negara. Produksi komoditas udang pada tahun 2014 mencapai 699.000 ton dan akan ditingkatkan menjadi 755.000 ton pada tahun 2015, dimana sekitar 70% dari target produksi tersebut adalah udang vaname. Akan tetapi, budidaya udang vanname secara intensif menimbulkan resiko terjangkit penyakit yang lebih tinggi. Sekitar 40% dari produksi udang hilang akibat infeksi penyakit, terutama penyakit yang disebabkan oleh serangan virus. Salah satu virus yang mengancam budidaya udang di dunia termasuk di Indonesia adalah Infectious myonecrosis virus (IMNV). Penelitian ini bertujuan untuk mengkaji pengaruh salinitas terhadap infeksi IMNV pada udang vaname serta mengkaji salinitas terbaik untuk pemeliharaan udang vaname yang diinfeksi IMNV. Penelitian dilakukan dengan metode eksperimen mengunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Udang uji yang digunakan berukuran berat ±2 g. Udang dipelihara dalam media bervolume 30 L pada akuarium yang berukuran 60 cm x 30 cm x 30 cm dengan salinitas media A 15 ppt, B 20 ppt, C 25 ppt, D 30 ppt dan tanpa infeksi 30 ppt. Udang uji dipelihara selama 24 hari, yaitu 7 hari aklimatisasi, 3 hari proses infeksi dan 14 hari pascainfeksi. Proses infeksi IMNV pada udang uji dilakukan melalui karkas. Setiap perlakuan diberi pakan 10% dari total biomassa. Hasil penelitian menunjukan bahwa perbedaan salinitas memberikan pengaruh terhadap perkembangan infeksi IMNV pada udang vaname. Salinitas memperlambat kemunculan gejala klinis. Mortalitas tertinggi pada perlakuan A (46,67%), kemudian perlakuan B (40,00%), perlakuan C (33,33%) dan terendah perlakuan D (23,33%), sedangkan perlakuan tanpa infeksi tidak mengalami kematian. Salinitas optimum untuk pemeliharaan udang vaname adalah 30 ppt.White shrimp L. vannamei (Boone,1931) is the one of fishery products are expected to generate income for the country. Shrimp commodity production in 2014 reached 699,000 tons and will be increased to 755,000 tons in 2015, of which approximately 70% of the production target is white shrimp. However, intensive cultivation of vannamei shrimp pose a risk of disease is higher. Approximately 40% of the shrimp production is lost due to infectious diseases, especially diseases caused by virus attacks. One virus that threatens shrimp farming in the world, including in Indonesia is Infectious myonecrosis virus (IMNV). This research was aimed to know the effect of salinity on the clinical symptoms and mortality of white shrimp infected by Infectious  myonecrosis  virus  (IMNV).  The  research  was conducted with experimental method by using the completely randomized design with 5 treatments and 3 replications. Shrimp test used heavy sized ±2 g. Shrimp maintained in media volume 30 L at the aquarium measuring 60 cm x 30 cm x 30 cm with a medium salinity of 15 ppt, B 20 ppt, C 25 ppt, D 30 ppt and without infection 30 ppt. Test shrimp reared for 24 days, it was 7 days of acclimatization, 3 day process of infection and 14 days after infection. IMNV infection process on shrimp test performed carcass. Each treatment was fed 10% of the total biomass. The results showed that the difference in salinity influence on the development of infection IMNV in shrimp. Salinity slow the appearance of clinical symptoms. The highest mortality in treatment A (46.67%), then treatment B (40.00%), treatment C (33.33%) and the lowest was treatment D (23.33%), whereas no infection treatment did not experience death. The optimum salinity for shrimp vaname maintenance is 30 ppt.
PENGARUH SALINITAS TERHADAP EFEK INFEKSI Vibrio harveyi PADA UDANG VANAME (Litopenaeus vannamei) Utami, Wiji; Sarjito, -; Desrina, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penerapan manajemen kualitas lingkungan merupakan langkah yang dapat ditempuh untuk mencegah penyebaran penyakit. Salah satu faktor lingkungan yang berperan penting adalah salinitas media budidaya. Pengaturan salinitas diharapkan mampu menjadi alternatif untuk meminimalisir infeksi Vibrio harveyi pada udang vaname. Penelitian ini bertujuan mengetahui pengaruh salinitas terhadap infeksi udang vaname (L. vannamei) yang diinfeksi V. harveyi serta mengetahui salinitas optimum yang dapat meminimalisir  infeksi V. harveyi pada udang vaname (L. vannamei). Uji pengaruh salinitas dilakukan dengan menginjeksikan bakteri V. harveyi sebanyak 0,1 mL dengan dosis 2 × 106 CFU/mL secara intramuscular dibagian abdominal kedua. Udang kontrol disuntik Phosphat Buffer Saline (PBS) pH 7.4 dengan volume sama. Udang  dipelihara selama 2 minggu didalam akuarium (vol air 24 L) yang dilengkapi dengan aerator menggunakan 5 perlakuan salinitas yaitu salinitas 15 ppt, 20 ppt, 25 ppt dan 30 ppt. dan 30 ppt (kontrol). Parameter utama yang diamati adalah jumlah ikan yang mati, gejala klinis total bakteri, histopatologi serta kualitas air.  Berdasarkan data parameter pengamatan  salinitas berpengaruh sangat nyata (P<0.05) dan (P<0.01) terhadap efek infeksi bakteri V. harveyi pada udang vaname (L. vannamei). Gejala klinis udang sakit yaitu nafsu makan berkurang, berenang miring dan lemah, mendekati gelembung udara, kaki renang, telson dan uropod kemerahan, nekrosis serta melanisasi pada segmen tubuh. Nilai kelulushidupan udang vaname yang diinfeksi V. harveyi tertinggi yaitu 70.83 % (Perlakuan D). Kelimpahan bakteri udang vaname pasca infeksi perlakuan D (1.86 × 104 CFU/mL), C (2.03 × 105 CFU/mL), B (2.55 × 107 CFU/mL) dan A (3.2 × 109 CFU/mL). Pengamatan histopatologi pada jaringan hepatopankreas udang yang diinfeksi V. harveyi terdapat nekrosis pada sel epitel tubula dan perbedaan jumlah sel B.  Implementation of environmental quality management is a step that can be taken to prevent the spread of disease. One environmental factor that is important is the cultivation medium salinity . Setting the salinity is expected to be an alternative to minimize infection of Vibrio harveyi in whiteleg shrimp. This study aims to determine the effect of salinity on infection whiteleg shrimp ( L. vannamei ) were infected with V. harveyi and determine the optimum salinity which can minimize infection V. harveyi in whiteleg shrimp ( L. vannamei ) . Test the effect of salinity is done by injecting the bacterium V. harveyi as much as a 0.1 mL dose of 2 × 106 CFU / mL intramuscularly second abdominal section . Injected control shrimp Phosphate Buffer Saline ( PBS ) pH 7.4 with the same volume . Shrimp maintained for 2 weeks in the aquarium (water vol 24 L) equipped with an aerator using 5 treatments salinity is 15 ppt salinity, 20 ppt, 25 ppt and 30 ppt. and 30 ppt (control). The main parameters measured were the number of dead fish, total clinical symptoms of bacterial, histopathological and water quality. Based on observations of salinity parameter data was highly significant (P <0.05) and (P <0.01) in the effects of bacterial infection V. harveyi in whiteleg shrimp (L. vannamei). Clinical symptoms are pain shrimp decreased appetite, swim tilt and weak, approaching air bubbles, swimming legs, telson and uropod redness, necrosis and melanisasi the body segments. Value whiteleg shrimp infected shrimp survival V. harveyi high of 70.83% (treatment D). whiteleg shrimp bacterial abundance of shrimp post-infection treatment D (1,86 × 104 CFU / mL), C (2,03 × 105 CFU / mL), B (2,55 × 107 CFU / mL) and A (3,2 × 109 CFU / mL). Histopathological observation on the network infected shrimp hepatopancreas V. harveyi are necrosis of tubule epithelial cells and differences in the number of B cells.
INFESTASI MONOGENEA PADA IKAN KONSUMSI AIR TAWAR DI KOLAM BUDIDAYA DESA NGRAJEK MAGELANG Putri, Sekar Mentari; Haditomo, Alfabetian Harjuno Condro; Desrina, -
Journal of Aquaculture Management and Technology Volume 5, Nomor 1, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Kerugian akibat infestasi ektoparasit termasuk monogenea tidak sebesar kerugian akibat infeksi organisme patogen lain seperti virus dan bakteri, namun infestasi tersebut dapat menjadi salah satu faktor predisposisi bagi infeksi organisme patogen yang lebih berbahaya, menimbulkan kerusakan organ luar berupa insang dan kulit, pertumbuhan lambat dan penurunan nilai jual ikan. Penelitian yang dilakukan bertujuan untuk mengetahui gejala klinis ikan yang terserang monogenea, jenis-jenis monogenea dan nilai intensitas dan prevalensi pada budidaya ikan konsumsi air tawar di desa Ngrajek. Penelitian ini dilakukan pada bulan Mei-Agustus 2015. Sampel ikan yang diperiksa sebanyak 90 ekor dengan ukuran panjang total panjang ikan nila 8,37±1,10 cm dan total berat 7,37±2,22 gr, total panjang ikan mas 9,54±1,48 cm dan total berat 9,78±2,27 gr dan total panjang ikan lele 9,73±1,21 cm dan total berat 4,57±1,35 gr. Pengambilan sampel dilakukan di 5 kolam budidaya. Pengamatan eksternal ikan dilakukan di laboratorium dan analisis data secara deskriptif dan kuantitatif. Hasil penelitian menunjukkan bahwa jenis parasit yang menginfestasi ikan air tawar adalah Dactylogyrus spp. dan Gyrodactylus spp. Total jumlah Dactylogyrus spp. yang ditemukan pada semua ikan adalah (247 individu) dan Gyrodactylus spp. (23 individu). Nilai intensitas Dactylogyrus spp. pada masing-masing ikan yaitu 7 ind/ekor pada ikan lele, 4 ind/ekor pada ikan mas dan 3 ind/ekor pada ikan nila, sedangkan nilai intensitas Gyrodactylus spp. di ikan lele 2 ind, di ikan mas 1 ind dan di ikan nila 1 ind. Nilai prevalensi Dactylogyrus spp. di ikan mas 70%, di ikan nila 56,7% dan di ikan lele 50%, sedangkan nilai prevalensi Gyrodactylus spp. di ikan lele 26,7%, di ikan mas 13,3% dan di ikan nila 10%. Gejala klinis yang tampak adalah memerah pada sirip dan pada beberapa ikan memiliki infeksi jamur. Ikan lele memiliki intensitas dan prevalensi tertinggi pada infestasi monogenea. Losses due to infestation of ectoparasites, including monogenea not as severe as a result of infection of pviruses and bacteria, but infestations can be a predisposing factor for microbial infection of pathogenic organisms which more dangerous, causing damage to organs beyond the form of gills and skin, slow growth and decline in value of selling fish. Research was aimed to determine the clinical symptoms of the fish infected by monogenea, types of monogenea and intensity values, the prevalence and dominance on the freshwater/fish farming in Ngrajek. This study was conducted in May-August 2015. The samples total 90 fishes consist of tilapia total length 8.37±1.10 cm and total weight 7,37±2,22 gr, carp total length 9.54±1.48 cm and total weight 9,78±2,27 gr, catfish total length 9.73±1.21 cm and total weight 4,57±1,35 gr. Sampling was conducted at 5 aquaculture ponds the external observation of fish was in laboratory. The results showed that monogenea parasites that infest freshwater fish are Dactylogyrus spp. and Gyrodactylus spp. The total number of Dactylogyrus spp. (247 individu) and Gyrodactylus spp. (23 individu). Dactylogyrus spp. intensity values in catfish was 7 ind, in carp was 4 ind and in tilapia was 3 ind, while Gyrodactylus spp. intensity values in catfish was 2 ind, in carp was 1 ind and in tilapia was 1 ind. Dactylogyrus spp. prevalence values in carp was 70%, in tilapia was 56.7% and in catfish was 50%, while Gyrodactylus spp. prevalence values in catfish was 26.7%, in carp was 13.3% and in tilapia was 10%. Clinical signs observed were reddish of fins. Same fish were also had fungi infection. Catfish had higher the intensity and prevalence of monogenea infestation.
SURVEI KEBERADAAN VIRUS WHITE SPOT SYNDROME (WSS) PADA CACING POLYCHAETA DI TAMBAK UDANG: STUDI KASUS DI KENDAL A H Desrina; Condro Haditomo; S B Prayitno
JURNAL LITBANG PROVINSI JAWA TENGAH Vol 9 No 2 (2011): Jurnal Litbang Provinsi Jawa Tengah
Publisher : Badan Perencanaan Pembangunan, Penelitian dan Pengembangan Daerah Provinsi Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36762/jurnaljateng.v9i2.280

Abstract

White Spot Syndrome (WSS) disease, caused by White Spot Syndrome Virus (WSSV), is an important shrimp disease in Kendal, one of many shrimp production area in Central Java Province. It has been indicated that polychaetes may be one of many vectors of WSSV. The objective of this research is to determine the occurrence and prevalence of WSSV infection in polychaetes obtained from shrimp pond with various culture condition. Pond A (semi intensive, monoculture P. Vannamei, pond size 2000 m2; 2); Pond B (extensive, monoculture P. Vannamei, pond size 5000 m2) and C (extensive, polyculture P. vannamei and tilapia Tilapia nilotica pond size 6000 m2). Sediment was obtained with PVC and sieved through a series of sieve shaker. Polychaetes found were counted and identified. Polychaetes was tested for WSSV infection with 1-step nested PCR and prevalence of infection was calculated. Polychaetes found consisted of 2 species namely Dendronereis sp and Nereis sp Polychaetes density (individu/ m2 ) for pond A, B and C are 102,1168 and 207 respectively. Prevalence of WSSV infection in Polychaetes from individual ponds is 29,4 % (A); 20% (B); and 12, 94 % (C). This prevalence is considered low compare to other similar research.
The Diversity of Causative Agent Associated With Bacterial Diseases on Catfish (Clarias gariepinus) with Molecular Based from Demak, Indonesia Sarjito Sarjito; A. Harjuno Condro Haditomo; Desrina Desrina; Restiana Ariyati; S. Budi Prayitno
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.723 KB) | DOI: 10.20884/1.oa.2018.14.2.553

Abstract

Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and  D14) that  were  caused  10–55% of fishes get sick  and  0–30%  fishes mortal.  On the other hand, there were 13 isolates do not cause both sick and mortality of fish.  On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.
Molecular Characteristics of Indonesian Isolate Enterocytozoon hepatopenaei Based on Sequence Analysis of 18S rRNA Genes Yohanes Kristiawan Artanto; Slamet Budi Prayitno; Sarjito Sarjito; Desrina Desrina; Alfabetian Harjuna Condro Haditomo
Journal Omni-Akuatika Vol 15, No 1 (2019): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12.995 KB) | DOI: 10.20884/1.oa.2019.15.1.694

Abstract

ABSTRACT Enterocytozoon hepatopenaei (EHP) infection have been reported as an obstacle of whiteleg shrimp (Penaeus vannamei) culture in Indonesia. However, understanding of the molecular characteristics of EHP species in Indonesia is not widely known. The aims of this study were to determine the identity and characters of DNA, and their phylogeny of EHP species from several different locations in Indonesia with specific references to 18S rRNA gene. The EHPs were collected from cultured P.vannamei from Lampung, Pangandaran, Sidoarjo, Banyuwangi, Probolinggo, Blitar, Makassar, and Lombok. Thirteen (13) samples were randomly selected to explore their gene characters through 18S rRNA gene sequencing. The primers used were EHP_F and EHP_1R. Parameter observed were DNA sequencing, nucleotide sequence homology with related available genes in the Gen Bank database, multiple sequence alignment, and reconstruction of genetic relationship trees. DNA sequence homology analysis showed that all samples had 99.89-100% similarity to Indian Enterocytozoon hepatopenaei (Accession Number MH259890.1 and MH260592.1). The alignment results illustrated that all EHP sequences of Indonesian isolates were 100% identical each other. The phylogenetic tree topology provided information that all sample accessions were in the same clade and spread evenly. The conclusion were that the Indonesian EHP species were identical (100%) and it could be said that they were genetically homogeneous. Keywords: Enterocytozoon hepatopenaei, 18S rRNA gene, Indonesia.
Pengaruh Dosis Terhadap Efektifitas Vaksin POM Vibrio alginolyticus 74 kDa pada Ikan Kerapu Macan Epinephelus fuscoguttatus Desrina Desrina; Arief Taslihan; Ambariyanto Ambariyanto; Budhi Kuncoro Jati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.635 KB) | DOI: 10.14710/ik.ijms.16.2.95-102

Abstract

Vibrio alginolyticus adalah bakteri patogen penyebab penyakit vibriosis pada ikan kerapu budidaya di Indonesia. Vaksin Protein Outer Membran (POM) V. alginolyticus telah terbukti imunogenik pada ikan kerapu. Penelitian ini bertujuan untuk menentukan pengaruh dosis vaksin  terhadap kemampuan POM V. alginolyticus 74 kDa dalam merangsang kerja sistem kekebalan spesifik ikan dan menentukan efek dosis terhadap perlindungan yang dihasilkan. POM(74 kDa) diisolasi dengan metoda sonikasi dan SDS-PAGE, dan dimurnikan dengan metoda elektroelusi. Vaksin diberikan dengan metoda suntik intraperitoneal ke ikan kerapu ukuran 8-10 cm (berat 1013 g) dengan dosis 0 (kontrol), 5, 10 dan 15 µg/0, 1 ml PBS/ 10 g ikan (n= 30 ekor/dosis).  Ikan kontrol disuntik dengan 0,1 ml PBS steril. Satu minggu kemudian ikan disuntik booster dengan cara dan dosis yang sama.  Dua  minggu  setelah  booster  dilakukan  uji  tantang  dengan  dengan  menyuntikkan  bakteri  Vibrio alginolyticus 8 secara intramuskular dengan dosis 0,1 ml X 109 sel/ml, dan ikan dipelihara selama 2 minggu. Jumlah ikan yang mati selama masa uji tantang dihitung untuk menentukan Relative Percentage Survival (RPS). Titer antibodi diukur sebelum percobaan dan setiap minggu selama penelitian. Ke 3 dosis yang diberikan efektif dalam merangsang respon kekebalan humoral ikan kerapu dan menghasilkan kekebalan yang melindungi yang hampir sama yang terlihat dari nilai RPS untuk dosis 5, 10 dan 15 µg masing masing 72, 87 dan 72%. Kata kunci: vaksin POM,  kerapu, dosis  Vibrio alginolyticus is a causative agent of  vibriosis of cultured grouper in Indonesia.  It has been reported  that the Outer Membrane Protein (OMP) of V. alginolyticus vaccine was immunogenic on grouper.  Vaccine dose is important in determining the ability of vaccine to conferred protective immunity. The objectives of the present research was to determine effect of vaccine doses on (1)  the specific immune response of grouper and (2) conferring protective immunity of grouper. OMP V. alginolyticus (74 kDa) was isolated by sonication and SDSPAGE, and purified by mean  electroelution. Vaccine was delivered by intraperitoneal injection to grouper juvenile (8 - 10 cm long  and  weigh10 - 13 g) in three doses;  0 (kontrol), 5, 10 dan 15 µg/0, 1 ml PBS/ fish (n= 30 fish/ dose).  Control fish were injected with 0,1 ml sterile PBS steril. One week later, booster  was given in the same manner as the primary vaccination. Two weeks following booster (week 4), fish were challenge with  V. alginolyticus 8 by intramuscular injection (0,1 ml X 109 sel/ml) and fish were maintained for two weeks. Fish mortality pos challenge test was counted to calcualte the Relative Percentage Survival (RPS). Antibodi titer was measured before vaccination and weekly for 4 week. All three doses tested were effective to  trigger the specific immune response of grouper dan conferred protective immunity with similar degree as shown by the RPS for dose 5, 10 dan 15 µg were 72, 87 dan 72% respectively. Key words: vaccine, OMP, grouper, dose.
Cacing Endoparasit Ikan Jeruk (Abalistes stellatus) dari Panfai Pekalongan Desrina Desrina
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 4 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.807 KB) | DOI: 10.14710/ik.ijms.12.4.233-238

Abstract

 Informasi cacing endoparasit ikan di Indonesia masih sedikit. Penelitian ini bertujuan untuk mengetahui profll cacing endoparasit ikan jeruk (Abalistes atellatus), salah satu jenis ikan konsumsi yang banyak ditangkap di perairan Pekalongan. Penelitian dilakukan dari bulan April sampai dengan Agustus 2001. Jumlah ikan jeruk yang diperiksa adalah 30 ekor, yang merupakan hasil tangkapan nelayan tradisional dan dipasarkan dalam keadaan segar di Tempat Pelelangan Ikan Batang dan Pekalongan. Pengamatan dilakukan dengan memeriksa organ organ dalam dan mata ikan satu persatu dibawah mikroskop. Parasityang ditemukan diawetkan dalam alkohol 70%. Parasit diidentirikasi pada waktu masih hidup dan setelah diawetkan. Semua ikan yang diperiksa terinfeksi cacing endoparasit. Cacing yang ditemukan terdiri atas 12 spesies Nematoda yaitu Anisakis simplex, Anisakis sp, Porrocaecum sp, Pseudoanisakis sp, Raphidascaris sp, Contracaecum sp, Camallanus sp, Procamallanus sp, Cucullanus sp, Philometra sanguinea, Philometra sp, Gnathostoma hispidium, dan I spesies Acanthocephala Acanthocephalus lucii. Parasit cacing paling banyak jenisnya ditemukan pada saluran pencernaan, diikuti oleh mesenteri dan rongga tubuh, hati, gonad dan dalam rongga mata. Prevalensi infeksi parasit yang tertinggi adalah Anisakis sp yaitu 80%. Intensitas per spesies cacing yang ditemukan relatif rendah berkisar 0,1- 5,85 ekor/ikan. Infeksi cacing tidak mempengaruhi berat gonad (r=-0,064, p<0,01) dan faktor kondisi ikan jeruk (r=0,0354, p<O,OI). jumlah cacing cendrung meningkat dengan bertambahnya berat ikan (r=0,476, p<0,05).Kata kuncl: cacing, endoparasit, ikan jeruk Information on endoparasitic helminths offish in Indonesia is very scanty. The objective of this research was to determine endoparasitic helminthes of trigger fish (Abalistes stellatus) from Pekalongan coast. A total of 30 trigger fish were examined during period April to August 2001. Fresh fish caught by traditional fishermen were obtained from Pekalongan and Batang fish auction. Each of internal organs and eyes cavity were examined for its worm under microscope. Worms found were preserved in 70% alcohol and identified alive as well as after being preserved. All offish examined were infected by worm. Parasites found consisted of 12 species of nematodes namely Anisakis simplex, Anisakis sp, Pseudoaniakis sp Porrocaecum sp, Raphidascaris sp, Contracaecum sp, Camallanus sp, Procamallanus sp, Cucullanus sp, Philometra sanguinea, Philometra sp, Gnathostoma hispidium, and I species Acanthocephala Acanthocephalus lucii. The most infected organs is alimentary tracts, followed by mesentery and body cavity, liver, gonad and eye cavity. Anisakis sp had the highest prevalence (80%). Intensity of each worm species was relatively low, range from 0,1- 5,85 worms/ fish. Worm infection did not affect the gonad weight (r=-0,064, p<0,0\) and fish condition factor (r=0,0354, p<0,01). The number of worms that infects trigger fish tended to increase as the fish weight increase (r=0,476, p<0,05). Key words : helminths, endoprasites, trigger fish
Parasit Ikan Kepe Kepe Garis (Shaetodon octotesciatus) dari Pantai Jepara Desrina Desrina
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 1 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1032.589 KB) | DOI: 10.14710/ik.ijms.7.1.27-32

Abstract

Informasi sebaran geografis parasit ikan hias laut di Indonesia sangat dibutuhkan untuk membuat peraturan tentang Ialulintas ikan dan penanganan penyakit. Penelitian ini bertujuan untuk mengetahui jenis parasit, prevelensi dan intensitas parasit ikan kepe kepe garis (Chaetodon octofasciatus) dari pantai Jepara, Jawa Tengah. Jumlah ikan yang diperiksa adalah 60 ekor. Ikan diperoleh dari nelayan desa Bandengan, Jepara. Pemeriksaan ikan dilakukan di Laboratorium Pengembangan Wilayah Pantai (LPWP) Fakultas Perikanan. dan Kelautan, Universitas Diponegoro di Jepara, selama Mei - Agustus 200I. lkan diperiksa dalam keadaan seger. Pengamatan ektoparasit dilakukan pada lamella insang, preparet ules lendir tubuh, dan sirip. Pemeriksaan endoparasit ikan dilakukan melalui pengamatan organ-organ dalam yang terlebih dahulu dibedah, dan kemudian diletakkan dalam petridish yang berisi air laut. Gejala klinis yang terlihat dicatat dan parasit yang ditemui diidentifikasi pada waktu segar dan setelah diawetkan. Prevalensi dan intensitas parasit juga dihitung. Parasit yang ditemukan terdiri atas tiga spesies ektoperasit yaitu Cryptocaryon irritans, Ergasilus, dan Actinocleidus sp.. Endoparasit yang ditemukan adalah Lecithocirium neopacificum dan Ichthyophonus hoferi. Prevalensi infestasi secara umum termasuk tinggi yaitu 78% akan tetapi prevalensi setiap spesies rendah sampai sedang , berkisar 6,67-40% dan intensitas parasit rendah yaitu 0,92- 25,32 individu. Kata kunci: parasit, ikan Kepe-Kepe. jepara  Data of geographical distribution of parasites of marine ornamentel fish in lndonesia is needed to construct regulation offish transportation. The purposes of this study were to determine parasites, prevalence and intensity of parasites of emperor butterfly fish (Chaetodon octofasciatus) from Jepara Coast. A total of 60 fresh fish were examined from May to August 2001. Fish were obtained from fishermen at Bandengan village Jepara and examined at the Coastal Development Laboratory (LPWP). Jepara. Ectoparasitic examination was carried out by examining gill lamellae, smear from the skin and fins. Fish was then necropsied. Each organ was placed in a petridish containing sea water and sliced. Parasites found were examined using a compound microscope. Any clinical signs was also noted. Parasites were identified alive as well as the preserved one. Prevalence and intensity of parasites were calculated. Parasites found consisted of three species of ectoparasites namely Cryptocaryon irritans, Ergasilus sp. and Actinocleidus sp. Endoparasites found were Lecithocirium neopacificum and Ichthyophonus hoferi, 78% of fish examined were infected which was relatively high. However, prevalence of each parasite species was ranged from low to moderate (6.67-40%) and intensity were fairly low (0 92-25.32 individu).Keywords: parasite, emperor butterfly fish, Jepara
Parasites Identification and Histopathology Changes on Blood Cookle (Anadara granosa Linnaeus, 1758) Yuni Karnisa; Desrina Desrina; Ita Widowati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 4 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.004 KB) | DOI: 10.14710/ik.ijms.24.4.171-178

Abstract

Blood cockle Anadara granosa is a popular sea food in Indonesia and potential for aquaculture. Currently, blood cockle aquaculture is at early stage, done by taking the larva from wild and raised them in the pond. Wild animal naturally carries parasites without outward clinical signs but can cause disease problem under aquaculture condition.  This study aimed to identify parasites and histopathology changes in wild A. granosa.  Blood cockles (n=90) were randomly collected during 3 months at three stations in the coastal waters of Bedono (Station 1: the intertidal area of the edge near to the mangrove area, station 2: the center of intertidal area, station 3: the area near to the sea), a village located on North Java coast and supplied blood cockle larva to the area. Parasites examination was conducted in the laboratory by macroscopic (observation of clinical symptoms) for ectoparasites and microscopic (using a microscope) for endoparasites. Histopathology preparation was done by taking three cockles at each station and cutting tissue that includes gills, foot, gonads, digestive tract and mantle. Organs were fixed in a 10% NBF solution, processed, mounted in paraffin and stained with hematoxylin and eosin. Parasites found were identified and the level of intensity and prevalence were calculated. There were three species of parasites found: Pinnotheres sp. (Intensity 1 ind/cockle; Prevalence: 3.33%), Perkinsus sp. (Intensity: 9.3 cell/cockle; Prevalence: 37.03%), Nematopsis sp. (Intensity: 4.25 cell/cockle; Prevalence: 29.62%). There were no histopathology changes on infected tissues which may relate to low diversity, prevalence and intensity of parasites found in this study.
Co-Authors A. Harjuno Condro Haditomo Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Ambariyanto Ambariyanto Arief Taslihan Arief Taslihan Budhi Kuncoro Jati Condro Haditomo Dicky Harwanto Fifiana Zulaekah Ita Widowati Kahan Dwi Supardi Mamluatul Lailiyah Mita Umiliana, Mita Monica Nanda Nida Qolbi Salma Rochani Peppy Dewi Fitriyanti Restiana Ariyati Restiana Wisnu Ariyati Rosa Amalia S B Prayitno S. Budi Prayitno Sarjito - Sekar Mentari Putri, Sekar Mentari Slamet Budi Prayitno Sulisyaningrum Sulisyaningrum Suryaningrum Susiani Tristiana Yuniarti Wiji Utami, Wiji Yohanes Kristiawan Artanto Yuni Karnisa Yunia Dharmastuti Widyaningsih