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Analisis Besi dalam Ekosistem Lamun dan Hubungannya dengan Sifat Fisikokimia Perairan Pantai Kabupaten Donggala Tahril, Tahril; Taba, Paulina; Nafie, Nursiah La; Noor, Alfian
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.257 KB) | DOI: 10.31258/jnat.13.2.105-111

Abstract

The objectives of the study are to describe the iron content of seagrass area ecosystem and its relation with nature of physico-chemical territorial water. The sampling of seagrass was conducted in purposive sampling technique for every status of the field and performed in three spots of the seagrass ecosystem region with reference to the depth of water and the current direction. Based on statistical tests of factorial and variance analysis indicate that the quality of physico-chemical territorial water in the coastal region of Donggala regency still very good, relatively. The result also showed, The Demand Oxygen and the turbidity gave a positive effect to Fe- seagrass improvement in its various association. Besides, the limiting factor for the low of Fe-seagrass is the height of salinity and water temperature. If the results were combined with another micro and macro mineral analysis will to become the basic for estimating the status of seagrass fertility.
Perbandingan Kemampuan Sedimen Rawa dan Sawah Untuk Mereduksi Sulfat dalam Air Asam Tambang (AAT) Fahruddin, .; Haedar, Nur; Nursiah, La Nafie
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 3, No 2 (2014): September
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.98 KB) | DOI: 10.35580/sainsmat3211212014

Abstract

Air Asam Tambang (AAT) dapat ditanggulangi dengan menggunakan sedimen sebagai sumber inokulum mikroba dalam mereduksi sulfat. Penelitian ini bertujuan untuk mengetahui pengaruh sedimen rawa dan sawah dalam peningkatan pH, penurunan kadar sufat dan jumlah mikroba pada air asam tambang. Perubahan pH diukur dengan menggunakan pH meter, kadar sulfat diukur dengan metode titrasi dan total mikroba dihitung dengan metode SPC (standar plate count). Hasil penelitian menunjukkan bahwa pemberian sedimen pada AAT dapat meningkatkan pH AAT dari 3 menjadi 6,263 pada sedimen rawa dan menjadi pH 6,557 setelah 30 hari. Pemberian sedimen juga mampu menurunkan kadar sulfat dari 563,15 ppm menjadi 327,41 ppm pada sedimen rawa dan menjadi 237,44 ppm pada hari ke-30. Penelitian ini juga menunjukkan bahwa jumlah mikroba pada sedimen rawa meningkat dari 2x105 sel/ml menjadi 37X105 sel/ml dan pada sedimen sawah juga meningkat dari 4,3X105 sel/ml menjadi 86X105 sel/ml pada hari ke-20.Kata Kunci: Sedimen, Air Asam Tambang, Bakteri Pereduksi Sulfat
Penggunaan Epiklorohidrin Dalam Meningkatkan Stabilitas Adsorben Kitosan Berikatan Silang Dan Kitosan Tercetak Ionik Kobal Muhammad Qaddafi; Abd. Wahid Wahab; Nursiah La Nafie; Paulina Taba
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1172.956 KB) | DOI: 10.24252/al-kimia.v2i2.1641

Abstract

The use of epichlorohydrin to improve the stability of chitosan adsorbent has been carried out. The purpose of this study was to determine the stability of the chitosan adsorbent after modified into epichlorohydrin cross-linked chitosan and ionic cobalt imprinted adsorbent chitosan cross-linked epichlorohydrin. Adsorbent prepared by homogeneous gel formation of chitosan in acetic acid medium and then modified into epichlorohydrin cross-linked chitosan beads and ionic cobalt imprinted adsorbent chitosan cross-linked epichlorohydrin. Stability of the adsorbent was determined by calculating the percentage of insoluble material after stirring the adsorbent in nitric acid medium at pH 1, 2, and 3 for 12 hours. The results of FTIR spectra show a shift of the absorption band of chitosan were compared with epichlorohydrin cross-linked chitosan and ionic cobalt imprinted adsorbent chitosan cross-linked epichlorohydrin. The use of epichlorohydrin as cross-linked agent may increase the stability of the adsorbent to maintain the percentage of insoluble material by 89% at pH 3 for epichlorohydrin cross-linked chitosan adsorbent and 96% at pH 3 for ionic cobalt imprinted adsorbent chitosan  cross- linked epichlorohydrin.
Isolasi Dan Karakterisasi Bakteri Simbion Larva Kupu-Kupu Cossus cossus Penghasil Enzim Selulase Maswati Baharuddin; Abd. Rauf Patong; Ahyar Ahmad; Nursiah La Nafie
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.729 KB) | DOI: 10.24252/al-kimia.v2i2.1653

Abstract

This study was conducted to characterize bacterial symbionts of butterfly larvae Cossus cossus capable of degrading cellulose. This study successfully purify and characterize isolates originating  from the intestine (CC1 and CC2), head (CC3), middle (CC4), and tail (CC5). From a qualitative test using 0.1% congo red gained the clear zone indicates that the bacteria are able to degrade cellulose. Based on the test temperature and pH on the growth of the data obtained CC5 isolates including isolates of thermophilic bacteria, while others including mesophilic bacteria. While based pH test all isolates were able to grow well at neutral pH. Based on the data obtained growth curve maximum bacterial growth at the 24th hour. Based on morphology and physiology test obtained bacteria genus Acinotobacter, Pseudomonas, and Bacillus.
PENGARUH SUHU DAN pH TERHADAP HIDROLISIS CMC OLEH ENZIM SELULASE DARI ISOLAT BAKTERI LARVA KUPU-KUPU COSSUS COSSUS Maswati Baharuddin; Abd. Rauf Patong; Ahyar Ahmad; Nursiah La Nafie
Teknosains Vol 8 No 3 (2014): Desember
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v8i3.1837

Abstract

Cellulase enzymes can be isolated from microorganisms that are resistant to high pH and temperature. Cellulase enzyme has a different character depending on the source and the enzyme environment. This study aimed to characterize the cellulase enzymes from bacteria isolates CC2 dan CC4 which includes the determination of the optimum pH and temperature of the enzyme activity. In this study, an enzyme produced from isolates of bacteria larva Cossus cossus by centrifugation cold 4 ° C with a speed of 3000 rpm for 15 min to obtain a crude extract of the enzyme cellulase. Determination of pH performed using sitrat acid buffer(3; 3,6; 4; 4,6; 5;5, dan 5) and phosphate buffer with pH variation (6,0; 6,5; 7,0; 7,5; dan 8,0), while for the determination of done an optimum temperature variations in temperature (20, 30, 40, 50, 60, 70, 80, dan 90) ° C enzyme activity were further tested using the Nelson-Samogy measured on a UV-Vis spekrofotometer at a wavelength of 545 nm. Result showed greatest activity isolat CC2 at pH 7,5 enzyme cellulase activity of 15,8806 x 10-4 U / mL while the optimum temperature of 70 ° C with the activity obtained at 19,4121 x 10-4 U / mL. Greatest activity isolat CC4 at pH 4 enzyme cellulase activity of 15,4069 x 10-4 U / mL while the optimum temperature of 70 ° C with the activity obtained at 20,3487 x 10-4 U / mL
MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS Ismail Marzuki; Alfian Noor; Nursiah La Nafie; M Natsir Djide
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.284 KB) | DOI: 10.20956/mca.v16i1.956

Abstract

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules
Distribusi Kuantitatif Logam Berat Pb dalam Air, Sedimen dan Ikan Merah (Lutjanus erythropterus) di Sekitar Perairan Pelabuhan Parepare Surahmi Usman; Nursiah La Nafie; Musa Ramang
Marina Chimica Acta Vol. 14 No. 2 (2013): Volume 14 No. 2 (2013)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.105 KB) | DOI: 10.20956/mca.v14i2.1189

Abstract

Research about the distribution of Pb in water, sediment and Red Snapper Fish (Lutjanus erythropterus) in the waters around the port of Parepare has been done using Atomic Absorption Spectrophotometer (AAS). Sample water, sediment and red snapper fish (Lutjanus erythropterus) was taken in three location; Ujung Lero, the residential people and traditional market.  The results showed that Pb levels in water ranged between 0.0380-0.8240 ppm, in sediments ranged between 38.9663-60.8982  mg/kg  dry  weight  while the red  snapper  fish  (Lutjanus  erythropterus)  ranged  between 4.0580-8.4590 mg/kg dry weight. Heavy metal of Pb most widely distributed in the sediment and in the water at least. The results have exceeded the threshold of water quality standards.
DETERMINATION AND DEGRADATION PYRENE FROM PAOTERE PORT SEDIMENTS WITH OXIDIZER KMnO4 Rachma Surya Masnawan; Nursiah La Nafie; Adiba Arief
Marina Chimica Acta Vol. 18 No. 1 (2017): Volume 18, No. 1 (2017)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.399 KB) | DOI: 10.20956/mca.v18i1.1831

Abstract

Paotere port is one of port in Makassar which functions as a stopover place of ships, also serves as fish market and fishermen settlements. Various activities of transportation, commerce and households occurred in the Paotere port area can be a source of pollution of pyrene compounds to the surrounding environment. Pyrene compounds that accumulate and can not be degrade will impact the environment and marine life. Determination pyrene in sediment from Paotere Port have been carried out using GC-MS by sonication using dichloromethane as solvent. Result show that there was no PAH in sediment of the three sampling stations. Pyrene compound chemically degradation was conducted by sonication for 1 hour using an oxidant KMnO4 concentration of  0.05 M, 0.07 M and 0.1 M. Results show that KMnO4 as oxidizing agent could to degrade the whole pyrene with concentration of 87,8554 ng/g in sediment.
THE POTENTIAL BIODEGRADATION HYDROCARBONS OF PETROLEUM SLUDGE WASTE BY CELL BIOMASS SPONGE Callysppongia sp. Ismail Marzuki; Alfian Noor; Nursiah La Nafie
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.121 KB) | DOI: 10.20956/mca.v16i2.7407

Abstract

The toxicity of petroleum sludge is a serious threat to marine life. Necessary concrete stepspetroleum contamination reduction through a search of potential marine materials degradetoxic components hydrocarbons. The sponge is one potential material reducing toxicproperties of petroleum contamination. The research objective was to determine thepotential Callyspongia sp sponge against the degradation of petroleum hydrocarbonsludge. Four variations of treatment used to determine the potential of sponge biomass,namely: the treatment I, II, III and IV. Indicators degradation is the formation of gas, thesmell fermentation, pH changes and increase in the absorbance of the suspension of mediadegradation. The level degradation each treatment is determined gravimetric method andthe known hydrocarbon component changes by using GC-MS. Unidentified gas, the smellof fermentation and changes in media of pH on average degradation occurs on the 15 daysof contact for all treatments. Data showed degradation occurs absorbance maximum at 20-25 days to contact. The highest degradation rates indicated by IV treatment (26.93 %), III(24.84 %), II (22.59 %), and I: (18.20 %). Found 20 kinds of components in the wastesludge aliphatic petroleum to form a homologous series nC10-nC30, and two aromaticcomponents, namely the 2.7-dimethyl naphthalene and 1.4-dimethyl azulene. Callyspongiasp sponge biomass concluded at the highest IV treatment degrading aliphatichydrocarbons, then treatment III, II and I, while the aromatic components are not degradedby biomass Callyspongia sp sponge at all treatments.
ANALYSIS METALS Fe AND Mn IN SEAGRASS ENHALUS ACOROIDES IN THE WATERS PASARWAJO BUTON Sabaniah Indjar Gama; Nursiah La Nafie; Seniwati Dali
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.15 KB) | DOI: 10.20956/mca.v16i2.7475

Abstract

The research aims to identify ferrous metals and manganese in seagrasses Enhalus acoroides. The sample used was obtained from the water pasarwajo buton. The sample was extracted, then the metal was analyzed using AAS. Metal concentrations (ppm) are Fe (143.667 mg / kg) and Mn (46.67 mg / kg), respectively. Metal content obtained below the maximum limit and seagrass Enhalus acoraides can be used as a bio-indicator of the presence of heavy metals