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All Journal MARINA CHIMICA ACTA Jurnal Natur Indonesia SAINSMAT Teknosains: Media Informasi Sains dan Teknologi Indonesia Chimica Acta Al-Kimia Chemistry Indonesian Journal of Chemical Research INTEK: Jurnal Penelitian

Nursiah La Nafie

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Hasanuddin University

Author-ID : 2671946

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Engineering Environmental Science Physics

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MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS Ismail Marzuki; Alfian Noor; Nursiah La Nafie; M Natsir Djide
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules

Distribusi Kuantitatif Logam Berat Pb dalam Air, Sedimen dan Ikan Merah (Lutjanus erythropterus) di Sekitar Perairan Pelabuhan Parepare Surahmi Usman; Nursiah La Nafie; Musa Ramang
Marina Chimica Acta Vol. 14 No. 2 (2013): Volume 14 No. 2 (2013)
Publisher : Hasanuddin University

Research about the distribution of Pb in water, sediment and Red Snapper Fish (Lutjanus erythropterus) in the waters around the port of Parepare has been done using Atomic Absorption Spectrophotometer (AAS). Sample water, sediment and red snapper fish (Lutjanus erythropterus) was taken in three location; Ujung Lero, the residential people and traditional market. The results showed that Pb levels in water ranged between 0.0380-0.8240 ppm, in sediments ranged between 38.9663-60.8982 mg/kg dry weight while the red snapper fish (Lutjanus erythropterus) ranged between 4.0580-8.4590 mg/kg dry weight. Heavy metal of Pb most widely distributed in the sediment and in the water at least. The results have exceeded the threshold of water quality standards.

DETERMINATION AND DEGRADATION PYRENE FROM PAOTERE PORT SEDIMENTS WITH OXIDIZER KMnO4 Rachma Surya Masnawan; Nursiah La Nafie; Adiba Arief
Marina Chimica Acta Vol. 18 No. 1 (2017): Volume 18, No. 1 (2017)
Publisher : Hasanuddin University

Paotere port is one of port in Makassar which functions as a stopover place of ships, also serves as fish market and fishermen settlements. Various activities of transportation, commerce and households occurred in the Paotere port area can be a source of pollution of pyrene compounds to the surrounding environment. Pyrene compounds that accumulate and can not be degrade will impact the environment and marine life. Determination pyrene in sediment from Paotere Port have been carried out using GC-MS by sonication using dichloromethane as solvent. Result show that there was no PAH in sediment of the three sampling stations. Pyrene compound chemically degradation was conducted by sonication for 1 hour using an oxidant KMnO4 concentration of 0.05 M, 0.07 M and 0.1 M. Results show that KMnO4 as oxidizing agent could to degrade the whole pyrene with concentration of 87,8554 ng/g in sediment.

THE POTENTIAL BIODEGRADATION HYDROCARBONS OF PETROLEUM SLUDGE WASTE BY CELL BIOMASS SPONGE Callysppongia sp. Ismail Marzuki; Alfian Noor; Nursiah La Nafie
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

The toxicity of petroleum sludge is a serious threat to marine life. Necessary concrete stepspetroleum contamination reduction through a search of potential marine materials degradetoxic components hydrocarbons. The sponge is one potential material reducing toxicproperties of petroleum contamination. The research objective was to determine thepotential Callyspongia sp sponge against the degradation of petroleum hydrocarbonsludge. Four variations of treatment used to determine the potential of sponge biomass,namely: the treatment I, II, III and IV. Indicators degradation is the formation of gas, thesmell fermentation, pH changes and increase in the absorbance of the suspension of mediadegradation. The level degradation each treatment is determined gravimetric method andthe known hydrocarbon component changes by using GC-MS. Unidentified gas, the smellof fermentation and changes in media of pH on average degradation occurs on the 15 daysof contact for all treatments. Data showed degradation occurs absorbance maximum at 20-25 days to contact. The highest degradation rates indicated by IV treatment (26.93 %), III(24.84 %), II (22.59 %), and I: (18.20 %). Found 20 kinds of components in the wastesludge aliphatic petroleum to form a homologous series nC10-nC30, and two aromaticcomponents, namely the 2.7-dimethyl naphthalene and 1.4-dimethyl azulene. Callyspongiasp sponge biomass concluded at the highest IV treatment degrading aliphatichydrocarbons, then treatment III, II and I, while the aromatic components are not degradedby biomass Callyspongia sp sponge at all treatments.

ANALYSIS METALS Fe AND Mn IN SEAGRASS ENHALUS ACOROIDES IN THE WATERS PASARWAJO BUTON Sabaniah Indjar Gama; Nursiah La Nafie; Seniwati Dali
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

The research aims to identify ferrous metals and manganese in seagrasses Enhalus acoroides. The sample used was obtained from the water pasarwajo buton. The sample was extracted, then the metal was analyzed using AAS. Metal concentrations (ppm) are Fe (143.667 mg / kg) and Mn (46.67 mg / kg), respectively. Metal content obtained below the maximum limit and seagrass Enhalus acoraides can be used as a bio-indicator of the presence of heavy metals

Co-Authors . Fahruddin Abd. Rauf Patong Abd. Wahid Wahab Abdul Wahid Wahab Abdul Wahid Wahab Adiba Arief Ahyar Ahmad Alfian Noor Alfian Noor Andi Nuraeni Indah Raya Ismail Marzuki Ismail Marzuki M Natsir Djide Maswati Baharuddin Muhammad Qaddafi Muhammad Zakir Musa Ramang Nur Haedar Paulina Taba Paulina Taba Prastawa Budi Rachma Surya Masnawan Ridhawati Ridhawati Sabaniah Indjar Gama Seniwati Dali Seniwati Dali Serly Tandigau Surahmi Usman Syaharuddin Kasim Tahril

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