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Optimasilisasi Waktu Fermentasi, Kadar Air dan Konsentrasi Cu2+ pada Produksi Lakase Trichoderma asperellum LBKURCC1 Secara Fermentasi Padat Batang Padi dalam Reaktor Labu Novia Sellyna; Miranti Miranti; Yuana Nurulita; Edy Saputra; Panca Setia Utama; Titania Tjandrawati Nugroho
Chimica et Natura Acta Vol 8, No 1 (2020)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v8.n1.26730

Abstract

Trichoderma asperellum LBKURCC1 adalah galur Trichoderma yang diisolasi dari tanah perkebunan cokelat di Riau yang mampu memproduksi lakase. Lakase merupakan enzim ligninolitik yang dapat mendegradasi lignin, sekaligus mengoksidasi senyawa fenol. Penelitian ini bertujuan mengoptimasi produksi lakase T. asperellum LBKURCC1 secara fermentasi padat (SSF) menggunakan batang padi sebagai penginduksi lakase, di dalam reaktor labu sederhana. Optimasi parameter fermentasi (waktu fermentasi, kadar air dan konsentrasi Cu2+)dilakukan menggunakan Central Composite Design (CCD) dengan Response Surface Methodology  (RSM). Hasil ANOVA menunjukkan bahwa model quadratik dipilih, dengan persamaan regresi Y= 64,19 - 6,71 X1 + 6,93 X2 - 15,65 X1*X1 - 7,11 X2*X2 - 15,40 X3*X3. Waktu fermentasi, kadar air dan konsentrasi Cu2+ sebagai CuSO4.7H2O ditemukan memiliki efek signifikan (p-value<0,05) terhadap aktivitas lakase yang diproduksi. Kondisi optimal untuk produksi lakase dengan penginduksi batang padi, secara SSF dalam reaktor labu, adalah 7 hari fermentasi, kadar air 67% dan konsentrasi CuSO4.7H2O 0,046 g/L. Aktivitas lakase yang diperoleh pada kondisi optimum adalah 65,3±0,7 mU per gram batang padi. Meskipun hanya meningkatkan aktivitas lakase 2% dari aktivitas pada center point, kondisi optimum tetap membuat proses menjadi lebih ekonomis dan efisien, karena memperpendek waktu produksi dari 8 hari menjadi 7 hari, dan mengurangi konsentrasi penambahan Cu2+.
Penerapan teknologi biopori vertikal dengan memanfaatkan larva Black Soldier Fly sebagai pengurai Andi Dahliaty; Halida Sophia; Yuana Nurulita; Sri Helianty
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.207-214

Abstract

The biopore infiltration hole is a hole with a diameter of 10 cm - 30 cm with a depth of 30 cm - 100 cm which is added with organic waste and will help soil biota form a biopore hole so that it can trap the air flowing around it. Black Soldier Fly larvae are able to accelerate composting and ordering of pores in the biopore infiltration holes. Perumahan Putri Indah Kulim RT 02 RW 12 Kelurahan Mentangor rarely finds open land semisization and house widening which causes a reduction in the surface of ground air infiltration so that during the rainy season puddles easily occur and during the dry season it reduces groundwater reserves. This activity aims to provide knowledge and insight into the community about biopore infiltration holes by utilizing Black Soldier Fly larvae and inviting the public to apply this technology. This service activity begins with the socialization of the biopore infiltration holes, then introduces the tools and materials as well as direct practice in the field. The results of this activity had a positive influence on the community and many community members who wanted to apply the biopore infiltration hole technology using Black Soldier Fly larvae. This is the first step in creating a clean and healthy environment, especially in Putri Indah Kulim Housing RT 02 RW 12 Mentangor Village.
Pengenalan Bunga Telang (Clitoria ternatea L.) di Desa Wisata Alam Sungai Masjid Kabupaten Dumai sebagai upaya membantu peningkatan Hospitality Ninik Nihayatul Wahibah; Delita Zul; Atria Martina; Yulminarti Yulminarti; Ennie Chahyadi; Yuana Nurulita
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.531-535

Abstract

Kelompok Sadar Wisata or Tourism Awareness Group (POKDARWIS) Desa Wisata Sungai Masjid Kabupaten Dumai have been improving their rural tourism. One of their services for tourists or guests is providing welcome drinks. Therefore, the objective of our program was to introduce butterfly pea, how to plant and how to utilize the flowers particularly for improving hospitality such as welcome drink and welcome kit. Butterfly pea (Clitoria ternatea L.) plants are known relatively easy to grow and possess chemical properties good for human health. We delivered our program to POKDARWIS Desa Wisata Sungai Masjid ss our partner through the oral presentation and demonstrated video. We also gave seeds and seedlings of butterfly pea to the participants. Our partners did not know about butterfly pea flower before participating in our program. They showed their enthusiasm and curiosity, particularly during the discussion session. Results of the questionnaire indicated that our program was useful to enhance their knowledge and provide inspiration to improve their life. One week later, after the training, the POKDARWIS had their program which adopted our program such as sowing and planting butterfly pea seeds and seedlings. Based on the results, we conclude that we have conveyed our program successfully and inspired our partners to produce butterfly pea-based food and beverages.
Value Added Eco Enzyme Sebagai Sabun Antiseptik Poppy Nurmayanti M; Yuana Nurulita; Jeni Wardi; Annisa Wulandari; Eka Lestari; Nesya Billa Sausan; Meisy Zahrati Afifah; Meisy Dwi Intan Sari; Niko Andriansyah; Rahmita Khairani Asrar; Yusma Dani; Eliza Khoirunisa; Rialdy
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 5 (2022): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v6i5.10997

Abstract

The purpose of community service is to educate the public in processing eco enzymes into products that have added value, as antiseptic soap. This activity was carried out as an effort to support the government's program in maintaining health through the movement of washing hands with soap. The activities method is training and mentoring carried out at the Bank Sampah Agrowisata, Rumbai District, Pekanbaru City. This activity includes two stages. First, the team conducted training and assistance in making eco-enzymes from citrun fruit peels, water, and palm sugar. The organic waste fermentation process is carried out for one month. Second, the team provided training and assistance in making antiseptic soap from eco enzyme. The results the effectiveness of antibacteria test at the Chemistry Laboratory FMIPA, Riau University showed that eco-enzyme that has been processed into soap tends to be more effective at killing bacteria than eco-enzyme. These results are expected to improve the community's economy to support zero waste and improve the skills and entrepreneurial of the community.
Pemanfaatan Bunga Telang (Clitoria ternatea L.) sebagai Teh yang bermanfaat bagi kesehatan masyarakat di Kampung Eduwisata Alam Sungai Masjid Kota Dumai Ninik Nihayatul Wahibah; Delita Zul; Atria Martina; Yulminarti Yulminarti; Yuana Nurulita; Ennie Cahyadi; Ahmad Huesean; Muhammad Rizky Darmawan; Ikhlasul Febrianto; Tania Aisyah Rinaldi; Nurkhasanah Putri Rakhman
Unri Conference Series: Community Engagement Vol 4 (2022): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.4.144-148

Abstract

Butterfly pea (Clitoria ternatea L.) known as ‘bunga telang’ is perennial plant originated from Indonesia. Butterfly pea flowers are edible flowers known to have active compounds such as antioxidants, anti-inflammatory, antibacterial, anti-cancer. These plants are grown at a lot of house-yard in Kampung Eduwisata Sungai Alam and are commonly used for natural dye for food and beverage. Therefore, objective of our programme was to introduce simple method for producing butterfly flower tea to Pokdarwis (Kelompok Sadar Wisata) Kampung Eduwisata Sungai Masjid. Result of the programme indicated that the participants were really enthusiastic involving in this training. Based on result of the questionnaire reveal that most of participants have positive perception to the training that contributing to improve their knowledge and skills in processing butterfly pea flowers. Percentage of participants who understand how to process the flower increase from 20% to 100% after the training. In addition, butterfly tea can be a commercially distributed that may improve economic level of the Sungai Masjid community. Furthermore, our programme motivates the community to take a part in conservation of natural resources.
Identifikasi Metabolit Sekunder Sekresi Jamur Lokal Tanah Gambut Riau Penicillium sp. LBKURCC34 Sebagai Antimikroba Yuana Nurulita; Yuharmen Yuharmen; Annisa Fitri; Iin Evita Sari; Dian Novita Sary; Titania Tjandrawati Nugroho
Chimica et Natura Acta Vol 10, No 3 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n3.45994

Abstract

Penicillium sp. LBKURCC34 adalah jamur yang diisolasi dari tanah gambut hutan primer di Giam Siak Kecil Bukit Batu (GSKBB), Cagar Biosfer di Provinsi Riau. Tujuan dari penelitian ini adalah untuk mengeksplorasi potensinya sebagai penghasil antimikroba. Metode dilakukan melalui produksi metabolit sekunder dalam media cair, ekstraksi dalam larutan etil asetat, dan pemisahan ekstrak kasar dengan Kromatografi Kolom (CC). Ekstrak kasar dan fraksi diidentifikasi dengan KLT dan HPLC dan diuji aktivitas antimikrobanya dengan metode difusi cakram. Untuk mengidentifikasi senyawa aktif dilakukan uji fitokimia dan metode KLT-Bioautografi. Produksi tiga liter media menghasilkan 0,4 gram (0,0133% b/v) ekstrak etil asetat yang diidentifikasi sebagai terpenoid dan fenolat; dan mengandung senyawa semipolar berdasarkan uji KLT dan HPLC. Hasil KLT setelah disemprot dengan p-anisaldehida 0,5% menunjukkan adanya senyawa peptaibol (peptida). Ekstrak etil asetat dapat menghambat pertumbuhan kelima mikroba patogen yang diuji namun hanya 11-45% dibandingkan dengan kontrol positif. Uji bioautografi terhadap Escherichia coli menunjukkan noda aktif zona bening pada Rf sebesar 0,67. Sayangnya, dari Fraksi 1-5 yang terdapat noda dengan Rf 0,67 masih belum menunjukkan aktivitas berarti. Penyelidikan lebih lanjut diperlukan untuk mengidentifikasi struktur senyawa aktif dari isolat lokal ini.