Article Per Year (5 Year)
p-Index From 2019 - 2024
0.444
P-Index
This Author published in this journals
All Journal Jurnal Hortikultura Jurnal Tanaman Industri dan Penyegar
Nur Kholilatul Izzah
Balai Penelitian Tanaman Industri dan Penyegar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Industrial & Manufacturing Engineering

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Pemilihan Tetua Persilangan pada Kubis (Brassica oleracea var. capitata) melalui Analisis Keragaman Genetik [Parental Line Selection in Cabbage (Brassica oleracea var. capitata) through Genetic Diversity Analysis] Nur Kholilatul Izzah; nFN Reflinur
Jurnal Hortikultura Vol 28, No 1 (2018): Juni 2018
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v28n1.2018.p33-40

Abstract

Kubis (Brassica oleracea var. capitata) merupakan salah satu jenis sayuran yang mempunyai nilai ekonomis tinggi. Untuk meningkatkan hasil panen kubis tiap tahunnya perlu didukung oleh tersedianya varietas unggul yang tahan penyakit, terutama penyakit busuk hitam dan akar gada yang dapat menggagalkan panen. Metode yang dapat diaplikasikan untuk merakit varietas unggul adalah melalui persilangan. Penelitian ini bertujuan untuk memilih kombinasi tetua persilangan yang ideal pada tanaman kubis melalui analisis keragaman genetik menggunakan marka SSR (Simple Sequence Repeats). Penelitian dilakukan pada bulan Februari sampai Mei 2013 di laboratorium Functional Crop Genomics and Biotechnology, Seoul National University, Korea Selatan menggunakan 16 genotipe kubis yang diperoleh dari perusahaan benih Joeun, Korea Selatan. Keragaman genetik 16 genotipe kubis dianalisis menggunakan 35 marka SSR polimorfik, dan selanjutnya digunakan untuk menentukan keragaman genetik berdasarkan metode UPGMA. Nilai jarak genetik antar genotipe diperoleh berdasarkan rumus 1-nilai kesamaan genetik. Hasil analisis keragaman genetik membagi 16 genotipe kubis menjadi dua kelompok heterotik utama pada nilai kesamaan genetik 65,2%. Berdasarkan hasil analisis keragaman genetik dan nilai jarak genetik diperoleh empat kombinasi tetua persilangan ideal, yaitu genotipe IMO-03 vs IMO-08 (nilai jarak genetik 43%) dan IMO-03 vs IMO-10 (nilai jarak genetik 39%) untuk karakter ketahanan terhadap penyakit busuk hitam, serta genotipe IMO-18 vs IMO-10 dan IMO-17 vs IMO-10 dengan nilai jarak genetik masing-masing 45% dan 44% untuk karakter ketahanan terhadap penyakit akar gada. Keempat kombinasi tetua tersebut dipilih karena terletak pada kelompok heterotik berbeda serta mempunyai nilai jarak genetik yang jauh sehingga diharapkan dapat meningkatkan peluang heterosis pada progeni yang dihasilkan.KeywordsBrassica oleracea var. capitata; Genotipe; Keragaman genetik; Kubis; Pemilihan tetuaAbstractCabbage (Brassica oleracea var. capitata) is one of vegetable that has high economic value. The availability of high-yielding varieties that are resistant to some diseases, particularly black rot and clubroot disease is needed in order to increase cabbage yield per year. The method which can be applied to assemble new varieties is through crossbreeding program. The objective of this research was to select the ideal combination of parental lines in cabbage through analysis of genetic diversity by using SSR markers (Simple Sequence Repeats). The research was conducted from February to May 2013 in the laboratory of Crop Functional Genomics and Biotechnology, Seoul National University, South Korea using 16 cabbage genotypes obtained from Joeun seed company, South Korea. The genetic diversity of 16 cabbage genotypes were analyzed using 35 polymorphic SSR markers, and then used to determine the genetic diversity based on UPGMA method. Meanwhile, genetic distance value among cabbage genotypes obtained by the formula of 1-value genetic similarity. The results of genetic diversity analysis divided 16 cabbage genotypes into two main heterotic groups at genetic similarity value of 65.2%. Based on the results of genetic diversity analysis and genetic distance value, we selected four combinations of ideal parental lines, namely genotype IMO-03 vs IMO-08 (genetic distance value of 43%) and IMO-03 vs IMO-10 (genetic distance value of 39%) for black rot disease resistance character, as well as genotype IMO-18 vs IMO-10 and IMO-17 vs IMO-10 with genetic distance value of 45% and 44%, respectively for club root disease resistance character. These four parental lines combination were selected as ideal parental combinations due to they were located on a different heterotic groups and has high genetic distance value, which is expected to increase the chances of heterosis in their progeny.
Analisis Kekerabatan Genetik Kultivar Kopi Arabika Berbuah Kuning dan Berbuah Merah Berdasarkan Marka SSR Nur Kholilatul Izzah; Enny Randriani; Dani Dani
Jurnal Tanaman Industri dan Penyegar Vol 2, No 3 (2015): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v2n3.2015.p113-122

Abstract

Yellow berry of Arabica coffee (AGK-1) cultivated in Garut is a local cultivar that potentially could be released as a superior variety. However, its genetic background has not been studied. Information of genetic background is one of the requirement in releasing of a new variety. The objective of this research was to analyze the genetic relationships of AGK-1 cultivar with 11 red berries Arabica coffee cultivars based on simple sequence repeats (SSR) markers to find polymorphisms and clustering. The research was carried out at the Molecular Laboratory of Indonesian Industrial and Beverage Crops Research Institute and Biology Molecular Laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, from January to March 2015. These twelve Arabica coffee cultivars were obtained from Garut Regency. A total of 12 SSR primers were used to investigate the genetic relationships of the plant. The result showed that 12 SSR markers were adequate to identify the relationships among 12 Arabica coffee cultivars. The genetic clustering obtained in this study is related to plant morphology, particularly plant growth characters, such as tall, semi dwarf and dwarf. AGK-1 cultivar genetically related to ABP-2, one of red berries cultivars that originated from Brazil. Both of these cultivars have the same growing type characters (i.e. semi dwarf). AGK-1 cultivar that has yellow berry color presumably derived from an open pollinated red berries parents, mainly ABP-2 cultivar. Therefore, AGK-1 is a unique cultivar that could be released as a local superior variety.
Variabilitas Genetik 11 Klon Kakao Lokal dari Sumatera Barat Berdasarkan Marka SSR Cici Tresniawati; Nur Kholilatul Izzah; Indah Sulistiyorini; Ilham Nur Ardhi Wicaksono
Jurnal Tanaman Industri dan Penyegar Vol 4, No 2 (2017): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v4n2.2017.p79-88

Abstract

Indonesia is the third largest cacao producing-country in the world and known having many superior local clones, such as that found in Lima Puluh Kota Regency, West Sumatra.  However, there is lack of information about genetic background of those local cacao clones.  This  study aimed to assess genetic variability of 11 local cacao clones collected from Lima Puluh Kota Regency, West Sumatra using SSR markers.  The research was conducted in the Integrated Laboratory, Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi, from August to November 2016.  The genetic variabilities of local cacao studied were compared with 9 national varieties as reference genomes.  Total genomic DNA of the plants was isolated using CTAB method. Cacao DNA was amplified using 18 SSR markers to determine their genetic variability.  Afterward, the amplified DNA was separated using 6% non-denaturing polyacrylamide gel electrophoresis. The result exhibited that 12 markers were polymorphic. Further analysis of these polymorphic markers using PowerMarker program revealed a total of 83 alleles were obtained from all cacao clones analyzed.  Meanwhile, PIC values ranged from 0.55 to 0.86 with an average of 0.70.  A genetic similarity matrix based on UPGMA revealed three main groups at 68% similarity coefficients. Interestingly, all of the 11 local cacao clones were clearly distinguished each other and also from the national varieties.  The result demonstrated the usefulness of SSR markers for discriminating local cacao clones. Further study is required to use these local clones in cacao breeding programs.
Penentuan Karakter Tanaman Yang Berpengaruh Terhadap Hasil Kemiri [Aleurites moluccana (L.) Willd.] Cici Tresniawati; Nur Kholilatul Izzah; Ilham Nur Ardhi Wicaksono; Indah Sulistyorini; Edi Wardiana
Jurnal Tanaman Industri dan Penyegar Vol 6, No 3 (2019): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v6n3.2019.p127-134

Abstract

Candlenut [Aleurites moluccana (L.) Willd.] is a perrenial plant that has high economic value. Information about vegetative and generative characters, as well as yield components that influence the yield is needed in the early stages of selection process in plant breeding. Selection of these characters can be done using Sequential Path Analysis (SPA) according to the plant development cycle, and then confirm the model using the Structural equation modeling (SEM) method. The aim of this study was to determine the vegetative characters and yield components that influence the candlenut production. The research was carried out at Pakuwon Experimental Station, Indonesian Industrial and Beverage Crops Research Institute, Sukabumi, from January to December 2018. Plant materials used in this study was 129 candlenut trees at 9 years old which were planted with a spacing of 10m x 10m. The data were analyzed using SPA followed by model confirmation using SEM method. The results showed that the production of seed was directly and positively influenced by the leaf petiole length, number of productive branches, seed weight, and number of fruits per tree, while the effect of stem height character is indirectly. Based on these results, plant selection based on vegetative characters such as leaf petiole length, number of productive branches, stem height character, number of fruits per tree and seed weight can be used to obtain high-yielding candlenut plants.
Keragaman Genetik Klon Lokal Kopi Robusta Asal Temanggung Berdasarkan Marka SSR Indah Sulistiyorini; Nur Kholilatul Izzah; Dani Dani; Budi Martono
Jurnal Tanaman Industri dan Penyegar Vol 8, No 3 (2021): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v8n3.2021.p141-150

Abstract

Kabupaten Temanggung merupakan salah satu sentra produksi kopi Robusta di Jawa Tengah dengan beragam klon kopi lokal yang potensial. Dari hasil eksplorasi diperoleh beberapa nomor klon lokal kopi Robusta yang berpotensi memiliki produktivitas tinggi dan diduga tahan terhadap hama/penyakit. Namun, klon kopi lokal tersebut belum diketahui secara jelas tingkat kemiripan genetiknya dengan klon-klon unggul yang sudah dilepas. Penelitian bertujuan untuk mengetahui keragaman genetik 29 klon lokal kopi Robusta asal Temanggung berdasarkan marka SSR. Penelitian dilakukan di Laboratorium Molekular lingkup Laboratorium Terpadu Balittri, mulai bulan Februari sampai Desember 2018. Penelitian menggunakan 29 nomor klon lokal yang berasal dari Temanggung dan dua varietas yang sudah dilepas, yaitu BP 42 dan BP 358, sebagai pembanding. Sebanyak 14 marka SSR yang digunakan dalam penelitian bersifat polimorfik dan mampu mengelompokkan klon kopi lokal asal Temanggung menjadi 5 grup besar dengan nilai koefisien kemiripan genetik sebesar 0,57. Empat klon kopi lokal Temanggung, yaitu kopi Putih Daun Lebar, Lokal, Tugusari Hijau, dan Tugusari Kuning berada dalam satu grup yang sama dengan klon pembanding BP 42 dan BP 358 pada grup I. Satu klon lokal (Tugusari Hijau) memiliki kemiripan genetik dengan klon pembanding BP 358 sebesar 0,91. Sementara itu, 25  klon lokal lainnya berada pada grup yang berbeda dengan klon pembanding. Klon lokal yang mempunyai jarak genetik yang jauh selanjutnya dapat dipilih sebagai kandidat klon unggul lokal dalam program pemuliaan tanaman kopi.
Co-Authors Budi Martono Cici Tresniawati Dani Dani Edi Wardiana ENNY RANDRIANI Ilham Nur Ardhi Wicaksono Indah Sulistiyorini Indah Sulistyorini nFN Reflinur