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PEMBUATAN JAS LAB DENGAN METODE SUPLIER SEBAGAI USAHA PENYEDIAAN JAS LAB BAGI PELAJAR DI BALI Prabawa, I Dewa Gede Putra; Madi E.S., I Gede Sukrawan; Aditya, Made Wira; Pratama, Kadek Agus Andika; Gede Bawa, I Gusti Agung
IPTEKMA Volume 2 No.1 - Desember 2010
Publisher : Bidang Kemahasiswaan Universitas Udayana

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Seiring banyaknya permintaan konsumen akan pakaian menyebabkan banyaknya jenis usaha pembuatan serta penyediaan pakaian berkembang di masyarakat. Umumnya mereka hanya menyediakan jasa pembuatan T-shirt, jaket, dan kemeja, hanya masih sedikit wirausaha yang menyediakan jasa pembuatan jas lab, sehingga berdampak sulitnya pelajar khususnya mahasiswa dalam memenuhi kebutuhan jas lab. Tujuan penulisan ini adalah mengetahui peluang usaha pembuatan jas lab di Bali, sehingga nantinya dapat membantu memenuhi kebutuhan jas lab mahasiswa serta pelajar SMP-SMA. Berdasarkan perhitungan harga yang dilakukan, dengan berwirausaha menggunakan sistem suplier ini, dihabiskan biaya pembuatan jas lab untuk bahan kain Nagata Drill sebesar Rp 76.000 dan bahan kain Oxford Rp 56.000 per potong. Harga jualnya adalah Rp 90.000 untuk jenis kain Nagata Drill dan Rp 70.000 untuk jenis kain Oxford, dengan harga tersebut diperoleh keuntungan Rp 14.000 per potong. Berdasarkan hasil survei harga rata-rata jas lab yang ada adalah Rp105.000. Hal ini menunjukkan prospek yang menjanjikan untuk usaha selanjutnya sebab dapat ditawarkan harga yang bersaing di pasaran.

EKSPLORASI EKSTRAK TUMBUHAN YANG MENUNJUKKAN AKTIVITAS ANTIJAMUR TERHADAP JAMUR CURVULARIA VERRUCULOSSA PENYEBAB PENYAKIT BERCAK DAUN PADA TANAMAN PADI (ORYZA SATIVA L.) I G. A. G. Bawa
Jurnal Kimia (Journal of Chemistry) Vol. 15, No.1, Januari 2021
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2021.v15.i01.p02

Eksplorasi bahan baku fungisida nabati untuk mengendalikan jamur Curvularia verruculosa penyebab penyakit bercak daun pada tanaman padi telah dilakukan. Dari 55 species tumbuhan yang diteliti menghasilkan 73 ekstrak etanol dari berbagai komponen tumbuhan. Beberapa ekstrak tumbuhan mampu menghambat pertumbuhan jamur C. verruculosa dengan sangat kuat, diantaranya ekstrak kulit kayu dan daun Michelia champaca dengan diameter zona hambatan masing-masing sebesar 30,07dan 22,07 mm, ekstrak kulit buah, daging buah dan daun Pangium edule Reinw (29,12;27,17; dan 20,16 mm), ekstrak daun Tamarindus indica L. (26,10 mm), ekstrak daun Michelia alba (26,03 mm) dan ekstrak daun Allamanda cathartica (23,00 mm). Hasil uji fitokimia menunjukkan bahwa mayoritas ekstrak tumbuhan yang memiliki daya hambat yang sangat kuat terhadap jamur C. verruculosa mengandung senyawa metabolit sekunder jenis terpenoid. Kata kunci: aktivitas antijamur, C. verruculossa, ekstrak tumbuhan, kandungan kimia ekstrak tumbuhan Exploration of raw materials for vegetable fungicides in controlling the Curvularia verruculosa fungal causing leaf spot disease in rice has been carried out. Extraction of various plant components of 55 plant species studied resulted in 73 ethanol extracts. Several plant extracts were able to very strongly inhibit the growth of C. verruculosa fungal, namely the extracts of bark and leaves of Michelia champaca showed inhibition zone diameters of 30.07 and 22.07 mm, respectively; the extracts of the frut rind, pulp and leaves of Pangium edule Reinw showed inhibition zone diameters of 29.2; 27.17; and 20.16 mm, respectively; the leaves extract of Tamarindus indica L., Michelia alba and Allamanda cathartica showed inhibition zone diameters of 26.10 mm; 26.03 mm, and 23.00 mm, respectively. The phytochemical test results indicated that the majority of plant extracts showing very strong inhibitory activity against C. verruculosa contain secondary metabolites of the terpenoid. Keywords: antifungal activity, chemical content of plant extracts, C. verruculossa, plant extracts

ISOLASI DAN IDENTIFIKASI SENYAWA GOLONGAN FLAVONOID PADA DAUN NANGKA (Artocarpus heterophyllus Lmk) DAN AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI Staphylococcus aureus Anak Agung Sagung Krisna Darmawati; I Gusti Agung Gede Bawa; I Wayan Suirta
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation, identification and antibacterial activity of flavonoid compounds from jackfruit leaf (Artocarpus heterophyllus Lmk) extract have been done in this research. Extraction of 500 g dry powder of jackfruit leaf using maceration method produced 31.20 g concentrated brownish green ethanol extract. Partition of 30.00 g the ethanol extract gained three concentrated extracts in n-hexane (4.46 g), chloroform (0.84 g) and n-buthanol (6.65 g) fractions. The phytochemical test showed that n-buthanol extract contained flavonoid compound which was active as antibacterial agent with 15.75 mm inhibition capacity. Column chromatography analysis on n-buthanol extract with mobile phase n-hexana: ethyl acetate: n-butanol (8:2:1) gave 8 fractions (FA, FB, FC, FD, FE, FF, FG, FH). Phytochemical testing showed that fraction FA and fraction FH belong to flavonoide compounds. Analysis of FA using UV-Visible gained 2 peaks at ? 323.40 nm (band I) and ? 285.60 nm (band II) which indicated the flavonoide groups of flavanone or dihydroflavonol. Analysis of FH using UV-Visible gained 2 peaks at ? 323.40 nm (band I) and ? 285.60 nm (band II) suggested the flavonoide groups of flavone or flavonol (3-OH substitution).` By using shiffting reagent the isolate FA was suggested to contain dihydroflavonol group and FH was suggested to contain flavone with hydroxyl groups at C-2’, C-4’.C-5’, C-6’, C-6, C-7, and C-8. Infrared analysis showed that the isolate had –OH, C=O, C-O, C=C aromatic, CH aromatic, and CH aliphatic groups. Antibacterial testing of flavonoid compounds of fraction A and fraction H on Staphylococcus aureus with a concentration of 10.000 ppm inhibited 10.50 mm for fraction A and 7.25 mm for fraction H.

ISOLASI DAN IDENTIFIKASI GOLONGAN SENYAWA TOKSIK DARI DAGING BUAH PARE (Momordica charantia L.) I G. A. Gede Bawa
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 2 Juli 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation and identification of toxic compounds were conducted to snake fruit (Momordica charantia L.).Maseration of 600 grams dried fruit in methanol resulted in 54.250 grams dark green extract which were toxic tobrine shrimp (Artemia salina Leach) with an LC50 = 74.99 ppm. When the extract was dissolved in water andpartitioned using n-hexane and chloroform three extracts were obtained. The n-hexane extract was found to be themost toxic to brine shrimp (LC50 = 130.439 ppm).Separation of the n-hexane extract using column chromatography resulted in 7 fractions. The most toxicfraction (F), LC50 = 33.83 ppm, was pure based on TLC.FTIR analyses showed that the active components of the extract contains triterpenoides characterized by CHaliphatic groups (CH2, CH3), binded O-H, C-O, C=C, and C=O. UV-Vis spectra showed absorption at 274,2 and432,8 nm suggesting an n * electronic transition.

PENENTUAN pH OPTIMUM ISOLASI KARAGINAN DARI RUMPUT LAUT JENIS Eucheuma cottonii I G. A. G. Bawa; A. A. Bawa Putra; Ida Ratu Laila
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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This research aimed to determine the optimum pH for the isolation carrageenan from Eucheuma cottoniiseaweed. The isolation was conducted at various pH : 7.5, 8.0, 8.5, and 9.0. A TLC using methanol : water (5:1) asmobile phase from standard carrageenan, gave result on Rf value 0.74 as equal to sample on treatment condition atpH 8.0 and 8.5. whereas, sample on treatment condition at pH 7.5 and 9.0 gave Rf value 0.72. In using ethanol :water (3:1) as mobile phase gave result on Rf value 0.75 from both standard and sample treated at 7.5, 8.0, and 8.5,while at pH 9.0 gave Rf value 0.74. Melting point test from standard gave range value 240-242, while for sample ontreatment condition at pH 7.5, 8.0, 8.5, and 9.0 gave range value each such 242-244, 241-243, 240-242, and 240-243.Compounds identification with solubility test method using various solvent medium obviously showed that both ofstandard and sample from various treatment condition were insoluble such as in aquades 200C, NaCl 25% 200C and800C, saccharosse 65% 200C and chloroform. Otherwise, they were soluble in aquades and saccharosse 65% withtemperature 800C. Infrared spectrum standard compound and sample from various treatment condition indicatedthat they were identical. Carrageenan isolation produce the largest number of rendemen at pH 8.5 with about34.65%, while the variant of carrageenan isolate was classified by kappa carrageenan.

ANALISIS SENYAWA TERPENOID ANTIJAMUR PADA FRAKSI AKTIF EKSTRAK KULIT KAYU CEMPAKA PUTIH (MICHELIA ALBA) DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY I G. A. G. Bawa; I G. A. G. C. A. Perbhawa
Jurnal Kimia (Journal of Chemistry) Vol. 14, No. 2 Juli 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2020.v14.i02.p06

Ekstrak kulit kayu cempaka putih (Michelia alba) mampu menghambat pertumbuhan jamur Curvularia verruculosa yang diisolasi dari daun padi yang terserang penyakit bercak daun. Pemisahan senyawa aktif telah dilakukan dengan metode kromatografi kolom menggunakan fase diam: silica gel dan fase gerak: campuran n-heksana-aseton (3:1). Hasil pemisahan diperoleh 10 fraksi gabungan, dimana 4 fraksi menunjukkan daya hambat yang sangat kuat, yaitu fraksi HAE (31,50 mm), HAF (30,13), HAG (48,50), dan HAH (34,88).Komposisi senyawa terpenoid pada keempat fraksi ini dianalisis dengan GC-MS. Senyawa ledenoxid-(II) dan isoaromadendrene epoxide ditemukan hampir pada semua fraksi aktif, sedangkan spathulenol ditemukan pada fraksi HAF dan HAG. Senyawa terpenoid lainnya yang juga ditemukan adalah cis-farnesol, trans-p-mentha-1(7),8-dien-2-ol, dan neoclovenoxid-alkohol hanya pada fraksi HAE, (-)-caryophyllene oxide dan tomentosin hanya pada fraksi HAG. Seluruh senyawa terpenoid yang terdeteksi merupakan senyawa dari kelompok seskuiterpen. Kata Kunci: Ekstrak, daya hambat, Currvularia verruculossa, seskuiterpen

SKRINING AWAL ANTITUMOR MELALUI PENDEKATAN UJI TOKSISITAS KANDUNGAN SENYAWA DALAM EKSTRAK n-HEKSANA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe) Wiwik Susanah Rita; I G. A. Gede Bawa; Ni Luh Putu Lilis Wirastiningsih
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 1 Januari 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation and identification of cytotoxic compounds from n-hexane extract of white turmeric rhizomes (Curcuma zedoaria (Berg.) Roscoe) has been performed. Extraction was done by maceration technique. Saponification reaction was applied to separate the fat with another lipid, separation and purification was then performed by chromatographic techniques. Toxicity tests performed by the method of Brine Shrimp Lethality Test (BSLT) using Artemia salina L. larvae, while the analysis of the most toxic isolates were performed by Gas Chromatography-Mass Spectroscopy (GC-MS).Extraction of 1700 g of dried white turmeric rhizome powder produced 39.68 g of concentrated n-hexane extracts. The results of toxicity tests with n-hexane extract of Artemia salina L. larvae was obtained LC50 values of 79.43 ppm. Saponification of n-hexane extract produced 9.18 g of n-hexane phase and 137.38 g water phase. Toxicity test indicated that n-hexane extract phase was the most toxic with the LC50 of 17.78 ppm. Then the active phase was separated and purified by gradient column chromatography and obtained 11 fractions. Fraction 11 was the the most toxic with the LC50 of 3.8 ppm. The column chromatography obtained two fractions (F11a and F11b), which F11b was the most active with LC50 of 3.5 ppm.The analysis of isolate by Gas Chromatography Mass Spectroscopy showed a mixture of compounds: tetradecane, hexadecane, 3-methylheptadecane, octadecane, 2-methyleicosane, n-docosane, and heneicosane.

AKTIVITAS ANTIOKSIDAN DAN ANTIJAMUR SENYAWA ATSIRI BUNGA CEMPAKA PUTIH (Michelia alba) I Gusti Agung Gede Bawa
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 1 Januari 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation of white tropical magnolia’s (Michelia alba) essential oil by maseration method was carried out. Antifungal activity test was conducted by measuring resistibility against Candida albicans growth, whereas antioxidant activity test was conducted with spectroscopy technique by using Difenilpikril hidrazin (DPPH) as antifree radical. Compound contents analysis of the isolate was carried out by Gas Chromatography-Mass spectroscopy (GC-MS).From 1000 grams of white tropical magnolia’s flower 3.18 grams n-hexane extract, 1.19 grams of chloroform extract, and 0.88 grams of ethyl acetate extract were gained. The retardation area for the three extracts were 0 mm. Free radical damping percentage were 24.47% for the first 5 minutes and 79.14% after 60 minutes for n-hexane extract, 21.72% for the first five minutes and 25% after 60 minutes for chloroform extract, 21.93% for the first five minutes and 39.87% after 60 minutes for ethyl acetate extract. Compound analysis was carried out for n-hexane extract that have the highest antioxidant activity. GC-MS analysis showed that white tropical magnolia’s flower had 6 major component i.e. 1,3-Benzioxole,5-(2-propenyl), cyclohexane; 1-ethenyl-1-methyl-2,4-bis (1-methylethenyl); Butanoic acid; 3-methyl-,2-phenylethyl ester; 9,12-Octadecadienoic acid, methyl ester; Tricosane; Pentacosane.

ISOLASI DAN IDENTIFIKASI SENYAWA MINYAK ATSIRI DARI TUMBUHAN SEMBUKAN (Paederia foetida L.) DENGAN METODE KROMATOGRAFI GAS-SPEKTROSKOPI MASSA (GC-MS) Dewa Gde Agung Yuda Pratama; I Gusti Agung Gede Bawa; I Wayan Gede Gunawan
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Sembukan is a wild plant that is often attached to the house fenced and has a lot of chemicals compound, including essential oil. This study aims to determine the active compounds as antibacterial of sembukan essential oil. One point four mililitres of essential oil was obtained by steam distillation. The essential oils were then tested for antibacterial activity against Escherichia coli and Stapylococcus aureus using the well diffusion method in gel. Identification of the essential oils conducted using Chromatography Gas – Mass Spectroscopy (GC-MS). The results of this study indicated that the essential oils did not have specific antibacterial activity against the bacteria tested. Compounds contained in the essential oils of sembukan were hydrogenated Linalool, Eugenol, Tetradecane, Heksadecane, and Dibutyl phthalate.

ANALISIS SENYAWA ANTIRADIKAL BEBAS PADA MINYAK DAGING BIJI KEPUH (Stercuria foetida L) I G. A. Gede Bawa
Jurnal Kimia (Journal of Chemistry) Vol. 4, No. 1 Januari 2010
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Analysis of anti freeradical compounds from Kepuh (Sterculia foetida L.) seed flesh oil has beenconducted. The isolation of kepuh seed flesh was carried out by maseration using n-hexane and ethanol. Antifreeradical test was carried out by spectroscopy method using DPPH as free radical. The analysis of the compoundsextracted accomplised was used Gas Column Mass Spectrometry (GC-MS).900 gram Kepuh seed yielded 250 mL oil n-hexane extract and 59,5 mL from ethanol extract. The resultof the anti freeradical test showed that the oil from n-hexane extract was not potencial as a anti freeradical agentwhile the oil from ethanol extract has a potency as a anti freeradical agent with damping percentage of 55,07%during 5 minutes and 85,05% during 60 minutes.The analysis using GC-MS, showed that ethanol extract oil from the flesh of kepuh seed contained 8 majorcomponent with relative concentration of methyl palmitate 3956,783 ppm (235,428 mg); palmitic acid 1837,414ppm (109,326 mg); ethyl palmitate 39288,783 ppm (2337,683 mg); (14Z,16Z)-ethyl octadeca-14,16-dienoate17062,660 ppm (1015,228 mg); (15Z,17Z)-methyl nonadeca-15,17-dienoate 19988,563 ppm (1189,319 mg); ethylstearate 4365,666 ppm (259,757 mg); (1Z,4Z)-1-methoxy-9,9,10,14-tetramethyl pentadeca-1,4-diena 22421,631 ppm(1334,087 mg); and (1Z,12Z,16Z)-1-ethoxy-6 methoxy octadeca 1,12,16-triena 78001,041 ppm (4672,262 mg).

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