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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Media Sains

I Wayan Suirta

Program Studi Kimia FMIPA Universitas Udayana, Jimbaran, Bali, Indonesia

Author-ID : 2921954

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

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Modifikasi Lempung Menjadi Adsorben dan Pemanfaatannya sebagai Penyerap Limbah Deterjen Suarya, Putu; Suirta, I Wayan
Jurnal Media Sains Vol 1, No 1 (2017): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

ABSTRAKSelektifitas lempung alam sebagai adsorben dapat diperbaiki melalui proses modifikasi lempung dengan proses aktivasi dan interkalasi. Modifikasi lempung akan diawali dengan proses aktivasi lempung menggunakan asam sulfat, yang bertujuan untuk membersihkan permukaan dan menghomogenkan kation antarlapis dari lempung. Selanjutnya proses interkalasi surfaktan akan menjadi lebih mudah terjadi apabila kation antar lapis telah homogen. Hasil karakterisasi terhadap lempung menunjukkan bahwa lempung yang telah dimodifikasi memiliki karakter yang lebih baik dilihat dari peningkatan luas permukaan spesifik dan keasaman permukaannya. Hasil peneltiannya menunjukkan terjadi peningkatan jumlah situs aktif dari lempung alam (A0) sebesar 3,9823 x 1020 atom/gram menjadi 10,947 x 1020 atom/gram pada lempung teraktivasi asam sulfat 2 M (AA) dan pada lempung teraktivasi asam sulfat 2 M dan terinterkalasi BKC 3% (AAB) sebesar 11,320 x 1020 atom/gram. Hasil uji kemampuan adsorpsinya terhadap limbah deterjen menunjukkan bahwa pada kajian variasi waktu adsorpsi, adsorben A0 mempunyai waktu kontak optimum 15 menit sedangkan AA dan AAB sampai dengan waktu 60 menit masih menunjukkan peningkatan daya adsorpsi terhadap limbah deterjen.Kata kunci : Modifikasi, Lempung, Montmorillonit, adsorpsi, limbah deterjen.ABSTRACTSelectivity of natural clay as an adsorbent can be improved through modification of clay with activation and intercalation process. Modification of clay will be preceded by the activation process using sulfuric acid, which aims to clean the surface and homogenizes the interlayer cations of clay. Selejuynya surfactant intercalation process will become easier to occur when cations between layers have been homogeneous. The results showed that the characterization of clay that has been modified to have better characters seen from the increase in specific surface area and the acidity of the surface. The Results showed that an increase in the number of active sites of natural clay (A0) of 3.9823 x 1020 atoms / gram to 10.947 x 1020 atoms / gram on activated clays 2 M sulfuric acid (AA) and the activated clay sulfuric acid 2 M and intercalated BKC 3% (AAB) amounted to 11.320 x 1020 atoms / gram. The test results on the adsorption ability of detergent waste shows that the study of time variation of adsorption, the adsorbent A0 have optimum contact time of 15 minutes while the AA and AAB up with 60 minutes still showed an increase in the adsorption capacity of the waste detergent.Key words : Modification, clay, montmorillonite, adsorption, waste detergent

Efektifitas Ekstrak Air Daun Gaharu (Gyrinop versteegii) Dalam Menurunkan Kadar Glukosa Darah Pada Tikus Wistar Hiperglikemia Nasution, Dina Munawaroh; Parwata, I Made Oka Adi; Suirta, I Wayan; Wasudewa, Kresna Murti
Jurnal Media Sains Vol 2, No 2 (2018): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/jms 3.v2i2.427

ABSTRAKPengobatan tradisional yang bersumber dari bahan alam saat ini sedang banyak dilakukan untuk penanganan diabetes mellitus. Penelitian terhadap ekstrak air daun gaharu (Gyrinop versteegii) ini bertujuan untuk mengetahui efektifitas penurunan kadar glukosa darah pada tikus wistar hiperglikemia. Hiperglikemia disebabkan adanya kenaikan jumlah radikal bebas. Senyawa antioksidan yang terkandung dalam daun gaharu berperan dalam menghambat stress oksidatif yang menjadi sumber penyakit salah satunya diabetes. Uji aktivitas antihiperglikemik dilakukan terhadap hewan uji coba berupa tikus wistar. Keadaan hiperglikemia pada hewan diperoleh melalui induksi aloksan secara intraperitroneal. Kelompok pembagian hewan uji dibagi menjadi lima dengan perlakuan dan dosis berbeda yaitu kelompok DP1 (100 mg/kgBB), kelompok DP2 (200 mg/kgBB), kelompok DP3 (400 mg/kgBB), kelompok DP4 (Kontrol Negatif), kelompok DP5 (Kontrol Positif). Pemeriksaan kadar glukosa darah dilakukan dengan glukotest selama interval hari ke-3, 7 dan 14. Data dianalisis secara statistika dengan One Way Anova dan Post Hoc Study menunjukkan nilai signifikansi (p<0,05) dalam menurunkan kadar glukosa darah. Hasil penelitian menunjukkan kelompok DP3 (400 mg/kgBB) memiliki efektifitas dalam penurunan kadar glukosa darah paling baik diantara kelompok dosis lain. Persentase penurunan kadar glukosa darah pada kelompok DP3 dan DP5 tidak berbeda jauh dengan nilai sebesar 74,78 % dan 75,80 %. Hasil ini didukung dengan kandungan total fenol dan flavonoid sebesar 6,0112 mg GAE/100 g dan 3,1533 mg QE/100 g.Kata kunci: daun gaharu (Gyrinop versteegii), kadar glukosa darah, tikus wistar, aloksan, hiperglikemiaABSTRACTTraditional medicine sourced from natural ingredients is currently being carried out for the treatment of diabetes mellitus. A study of gaharu leaves water extract (Gyrinop versteegii) was conducted to determine the effectiveness of decreased blood glucose level in hyperglycemic wistar rats. Hyperglycemia is caused by an increasing number of free radicals. Antioxidant compounds contained in gaharu leaves play a role in inhibiting oxidative stress which is the source of disease as diabetes. An antihyperglycemia activity test was performed on experimental animal as wistar rat. Hyperglycemia in the animals is obtained through intraperitroneal alloxan induction. The animal distribution group was divided into five different treatments and doses of DP1 (100 mg / kgBB), DP2 (200 mg / kgBB), DP3 (400 mg / kgBB), DP4 (Negative Control), DP5 (Positive Control). Blood glucose level examined by blood glucose examiner (Glucotest) during the 3rd, 7th and 14th day intervals. Data were analyzed statistically by One Way Anova and Post Hoc Study showed significant (p<0,05) in decreasing blood glucose level. The results showed that DP3 (400 mg / kgBB) has the best effectiveness indecreasing blood glucose levels than other dosage group. Decreased blood glucose percentage of DP3 and DP5 has close differences, it is 74,78 % and 75,80 %. These results were supported by total phenol and flavonoid content of 6.0112 mgGAE/100g and 3.1533 mgQE/100 g.Keywords: Gaharu leaves, antihyperglycemic, blood glucose concentration, wistar rat, alloxan.

AKTIVITAS ANTIBAKTERI MINYAK ATSIRI RIMPANG JERINGAU (Acorus calamus Linn.) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus Wiwik Susanah Rita; I Wayan Suirta; Putu Prisanti Putri Utami
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 5 No 2 (2017)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Isolasi minyak atsiri dari rimpang jeringau (Acorus calamus Linn) dan uji aktivitas antibakteri terhadap bakteri Escherichia coli (E. coli) dan Staphylococcus aureus (S. aureus) telah dilakukan. Ekstraksi minyak atsiri dilakukan dengan metode destilasi uap, uji aktivitas antibakteri dilakukan dengan metode sumur difusi, dan identifikasi senyawa dilakukan dengan kromatografi gas-spektroskopi massa (KG-SM). Rendemen minyak yang dihasilkan sebesar 0,1653% (b/b) dengan berat jenis 1,066 g/mL. Minyak atsiri dengan konsentrasi 10% (v/v) menunjukkan aktivitas penghambatan yang kuat terhadap pertumbuhan E. coli dan S. aureus dengan diameter hambat masing-masing sebesar 11,33 mm dan 13,57 mm. Nilai konsentrasi hambat minimum (KHM) minyak atsiri terhadap E. coli dan S. aureus masing-masing sebesar 4 dan 0,4% dengan zona hambat berturut-turut 6,67 dan 8,83 mm. Kandungan senyawa utama dalam minyak atsiri rimpang jeringau adalah euasaron dan asaron yang telah diketahui memliki aktivitas sebagai antibakteri. Kata kunci: Minyak atsiri, Acorus calamus Linn, Antibakteri, Escherichia coli, Staphylococcus aureus ABSTRACT: Isolation of essential oils from rhizome of Jeringau (Acorus calamus Linn) and the antibacterial activity assay against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) has been performed. Essential oil extraction was done by steam distillation method, antibacterial activity test was conducted by diffusion wells, and compound identification was conducted by gas chromatography-mass spectroscopy (GC-MS). The essential oils with a concentration of 10% (v/v) showed a strong inhibitory activity against the growth of E. coli and S. aureus with diameter inhibition of 11.33 mm and 13.57 mm respectively. Minimum inhibitory concentration (MIC) of the oils against E. coli and S. aureus was 4 and 0.4% respectively with the inhibition zone of 6.67 and 8.83 mm. The main compounds of the oils were euasaron and asaron known having antibacterial activity.

AKTIFITAS ANTIINFLAMASI TOPIKAL MINYAK ATSIRI DAN EKSTRAK ETER TUMBUHAN TENGGULUN, PROTIUM JAVANICUM, BURM TERHADAP MODEL INFLAMASI KULIT PADA TIKUS I Wayan Suirta; Ni Made Puspawati; I.A. Raka Astiti Asih
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 4 No 1 (2016)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Tenggulun (Protium javanicum, Burm ) secara tradisional telah dimanfaatkan masyarakat Bali sebagai tanaman obat untuk mengobati bengkak. Penelitian ini bertujuan untuk menentukan aktivitas antiinflamasi ekstrak eter kulit batang dan minyak atsiri daun tengulun pada udem (inflamasi) telinga tikus yang diinduksi TPA (12-O-tetradecanoylphorbol-13-acetate) secara topikal. Ekstraksi dilakukan dengan maserasi dan isolasi minyak atsiri dengan distilasi uap. Pada penelitian ini digunakan rancangan acak lengkap dengan sembilan kelompok perlakuan dengan masing-masing perlakuan terdiri dari lima ekor tikus. Inflamasi diukur 6 jam setelah perlakuan. Hasil triturasi 180g ekstrak etanol kulit batang tenggulun diperoleh 6,30 g ekstrak dietil eter dan hasil distilasi uap 4,5 kg daun segar diperoleh 2,5 g minyak atsiri. Hasil uji fitokimia ekstrak kulit batang tenggulun mengandung senyawa golongan terpenoid dan minyak atsiri mengandung senyawa flavonoid, terpenoid, dan steroid. Hasil uji aktifitas antiinflamasi menunjukkan bahwa persentase hambatan peradangan dari ekstrak tenggulun didapatkan : KEI (58,34%) , KEII (54,17%) , KEIII (45,85%) dan KAI (50,02%) yang menunjukkan hambatan yang tidak berbeda secara bermakna dengan hambatan yang diberikan oleh kontrol positip KP (62,50%). Ekstrak eter 12 mg dan 20 mg masih memberikan hambatan peradangan yang lebih besar dari minyak atsiri 20 mg. Hasil uji histologi menunjukkan ekstrak eter dan minyak atsiri dapat menghambat migrasi sel radang lebih dari 50%. Jumlah sel radang yang bermigrasi ke daerah peradangan sebanyak 17 sel untuk ekstrak eter dosis 20 mg/telinga dan 20 sel radang untuk minyak atsiri dosis 20 mg/ telinga. Dengan induksi TPA jumlah sel radang yang bermigrasi ke daerah peradangan sebanyak 64 selABSTRACT: Protium javanicum, Burm (Tenggulun) has been used traditionally in Bali as medicines to treat inflamation. This research aimed to evaluate topical anti-inflammatory activity of stem bark ether extract and essential oil of leaves on ear edema rats induced by TPA (12-O-tetradecanoylphorbol-13-acetate). Extraction of stem bark was done by maceration and the essential oil was produced by steam distillation. Complete Randomized Design with nine treatment groups was used in this study. Each of the treatment had five repetitions. The inflammation was measured after 6 hours of treatment. Trituration of 180 g crude ethanol extract with ether gave 6.30 g ether extract and steam distillation of 4.5 kg fresh leaves yielded 2.5 g of the essential oil. Phytochemical study showed that stem bark ether extract only contained triterpenoid while the essential oil of leaves contained flavonoid, terpenoid, and steroid compounds. The yield of % inhibited antiinflamatory activity showed that KEI (58,34%), KEII (54,17%), KEIII (45,85%) and KAI (50,02%) was insignificantly different to positive control dexamethason which inhibited inflammation by 62.50%. The result of histology test showed that ether extracts and essential oils can inhibit the formation of inflammatory cells of more than 50%. Dosis of 20 mg/ear of ether extract (formed 17 inflammatory cells) and 20 mg/ear of essential oil (formed 20 inflammatory cells) can inhibit the formation of inflammatory cells induced by TPA (formed 64 of inflammatory cells).

UJI AKTIVITAS ANTIINFLAMASI FRAKSI N-HEKSAN DAUN CENDANA (Santalum album Linn.) TERHADAP OEDEM TIKUS PUTIH JANTAN GALUR WISTAR YANG DIINDUKSI KARAGENAN Ni Made Puspawati; I Wayan Suirta; Ni Luh Putu Mega Wahyuni; Ni Ketut Ratnayani
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 5 No 2 (2017)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Cendana (Santalum album L.) umumnya digunakan sebagai obat tradisional untuk mengobati inflamasi (bengkak). Penelitian ini bertujuan untuk menguji aktivitas antiinflamasi fraksi n-heksan ekstrak daun cendana (Santalum album L.) terhadap udema pada telapak kaki tikus putih jantan galur Wistar yang diinduksi karagenan dan mengidentifikasi senyawa aktifnya. Pada pengujian aktivitas antiinflamasi digunakan 5 kelompok tikus yaitu kelompok kontrol positif (na-diklofenak 5 mg/kg BB), kelompok kontrol negatif (CMC 1%) dan 3 kelompok perlakuan (dosis fraksi n-heksan 125 mg/kg BB, 250 mg/kg BB, dan 500 mg/kg BB). Hasil uji aktivitas antiinflamasi menunjukkan pada menit ke-360 fraksi n-heksana pada dosis 125 mg/kg BB dan 250 mg/kg BB mampu menghambat udema telapak kaki tikus berturut-turut sebesar 80,39% dan 82,35% lebih tinggi dari kontrol positif yaitu 66,66%. Sedangkan pada dosis yang lebih tinggi 500 mg/kg BB aktivitasnya menurun dan lebih rendah dari kontrol positif yaitu sebesar 44,12%. Hasil uji One Way ANOVA menunjukkan bahwa pemberian dosis 125 mg/kg BB dan dosis 250 mg/kg BB tidak memiliki perbedaan bermakna dengan kelompok kontrol positif, sedangkan untuk dosis 500 mg/kg BB memiliki tidak memiliki perbedaan bermakna dengan kontrol negatif. Analisis GC-MS menunjukkan bahwa fraksi n-heksan daun cendana mengandung senyawa 2,2,4-trimetil-1,3-pentanadiol diisobutirat; metil palmitat; metil linoleat; metil oleat; di(2-etilheksil) terephtalat; dan asaron. ABSTRACT: Sandalwood (Santalum album L.) is commonly used as a traditional medicine to treat inflammation. The aim of this research were to evaluate antiinflammatory activity of n-hexane fraction of cendana leaves extract and to characterise its active compounds. The anti-inflammatory activity test was conducted using 25 Wistar rats which were divided into five groups: group 1 treated with carrageenan (control negative), group 2 treated with standar drug (sodium diclofenac) whereas group 3,4, and 5 treated with different doses (125, 250, and 500 mg/kg/BW) of n-hexane fraction of santalum album leaves extract along with carrageenan respectively. The active constituent was charactersised using GC-MS (Gas Chromatography-Mass Spectrometer). The anti-inflammatory test demonstrated that the n-hexane fraction of santalum album leaves reduced carrageenan induced rats paw edema in a dose dependent manner. The n-hexane fraction at dose of 125 and 250 mg/kg BW showed potent inhibition of inflammation after 6 hours which can inhibit inflammation by 80.39 and 82.35% respectively as compared to positive control (66.66%). Further increase in dose i.e 500 mg/kg BW decreased the activity to 44.12%. Statistical analysis showed that antiinflammatory activity of n-hexane fraction at doses 125 mg and 250 mg/kgBW did not significantly different to control positif while at dose of 500 mg/kgBW did not significantly different to control negative. The active compounds were tentaviley identified as 2,2,4-trimethyl-1,3-pentandiol diisobutyrates, hexadecanoic acid, methyl ester; octadeca,9,12-dienoic acid, methyl ester, 9-octadecenoic acid, methyl ester, terepthalic acid, 2-ethylhexyl undecyl ester, and asarone.

AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN CENDANA (Santalum album L.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli Ni Made Puspawati; I Gede Tangkas Mei Yasa; I Wayan Suirta
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 2 (2018): Volume 6, Nomor 2, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Cendana (Santalum album L.) merupakan tanaman endemik Nusa Tenggara Timur yang secara tradisional telah digunakan untuk mengobati berbagai macam penyakit termasuk infeksi. Penelitian ini bertujuan untuk menentukan aktivitas antibakteri fraksi n-heksana, kloroform, dan n-butanol ekstrak daun cendana (Santalum album L.) terhadap bakteri Staphylococcus aureus dan Escherichia coli serta menentukan konsentrasi hambat minimum dan mengidentifikasi komponen senyawa pada fraksi paling aktif. Serbuk daun cendana dimaserasi dengan metanol dan ekstrak metanol yang diperoleh kemudian difraksinasi berturut-turut dengan n-heksana, kloroform, dan n-butanol. Masing-masing fraksi yang diperoleh diuji aktivitas antibakterinya dengan metode sumur difusi. Hasil penelitian menunjukkan fraksi n-heksana mampu menghambat pertumbuhan bakteri Staphylococcus aureus dengan diameter zona hambat sebesar 19,30 mm dan memberikan daya hambat minimum 7,5 mm pada konsentrasi 17% (b/v), tetapi tidak menunjukkan aktivitas antibakteri terhadap Escherichia coli. Sementara untuk fraksi kloroform dan n-butanol tidak menunjukkan aktivitas antibakteri tehadap kedua bakteri uji. Pemisahan fraksi aktif n-heksana dengan kromatografi kolom dengan eluen n-heksan:etil asetat (8:3), memberikan 6 fraksi gabungan (A,B,C,D,E,F), dan fraksi B menunjukkan aktivitas antibakteri relatif paling besar dengan diameter zona hambat 10,60 mm. Hasil identifikasi fraksi aktif B dengan GC-MS menunjukkan terdapat lima belas senyawa dan tiga diantaranya dengan persentase area terbesar yaitu lupeol, nerolidol, dan sitostenone yang merupakan senyawa golongan terpenoid diduga berkontribusi terhadap aktivitas antibakterinya. Kata kunci: cendana, antibakteri, Staphylococcus aureus, Escherichia coli, terpenoid. ABSTRACT: Sandalwood (Santalum album L.) is an endemic plant of East Nusa Tenggara whicht has been traditionally used to treat various diseases including infection.. The aim of this research was to determine antibacterial activity of n-hexane, chloroform, and n-buthanol fractions of methanol cendana leaf extract toward Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) bacteria and minimum inhibitory concentration of active fraction and to identify chemical consituents of the active fraction. Cendana leaf powder was macerated with methanol to yield crude methanol extract which was then fractionated into n-hexane, chlorofrom, and n-buthanol fractions respectively. Each fraction was tested for its antibacterial activity using well diffusion method. The most active fraction was separated using column chromatography and identified using GC-MS (Gas Chromatography-Mass Spectrometer). The antibacterial activity test showed the n-hexane fraction was able to inhibit the growth of S.aureus with inhibition zone diameter of 19.30 mm and minimum inhibitory concentartion at 17 % (w/v) with inhibition zone diameter of 7.50 mm but it did not inhibit the growth of E.coli, while chloroform and n-buthanol fractions did not inhibit the growth of both S. aureus and E. coli. The separation of n-hexane active fraction on column chromatography eluted with n-hexane: ethyl acetate (8:3) gave six fractions (A,B,C,D,E,F) and fraction B was the most relatively active toward S.aureus with inbition zone diameter of 10.60 mm. Identification of fraction B showed fifteen compounds and three of them with high percentage area were identified as lupeol nerolidol and sitostenone, a terpenoid compounds, which may contribute for the actibacterial activity.

PEMANFAATAN TEH KOMBUCHA SEBAGAI OBAT HIPERURISEMIA MELALUI PENURUNAN KADAR ASAM URAT DAN MALONDIALDEHID Ni Luh Putu Kartika Mardiani; Ida Bagus Putra Manuaba; I Wayan Suirta
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Research has been conducted to determine the decrease in uric acid levels and malondialdehyde (MDA) of rats hyperuricemia after being given kombucha tea. The research was carried out with the draft post-test control group design. Kombucha tea with various fermentation time of 4, 8 and 12 days were given to hyperuricemia rats with doses of 10 mL/kg body weight and 40 mL/kg body weight. The results showed that kombucha tea can lower uric acid levels and MDA with the most significant decrease showed by the 8 day fermented kombucha tea with a dose of 40 mL/kg body weight. The decreases were 2.00±0.09 mg/dL and 1.68±0,34 umol/L respectively.

ISOLASI DAN IDENTIFIKASI SENYAWA YANG BERPOTENSI SEBAGAI ANTITUMOR PADA DAGING BUAH PARE (Momordica charantia L.) Wiwik Susanah Rita; I W. Suirta; Ali Sabikin
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 1 Januari 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Isolation and identification of the compound which has a potency as antitumor from bitter melon have beencarried out. Extraction was conducted n-hexane, chloroform, and ethanol respectively using each extracts obtainedwere examined with brine shrimp lethality test. The most toxic extract was ethanol extract (LC50 223 ppm).Separation and purification of the compounds from the ethanol extract were conducted by column chromatogaraphyusing a gel silica 60 as the stationary phase and benzene : acetic acid ( 8:2) as the mobile phase. This yielded 3fractions. Then the fractions were examined with brine shrimp lethality test and the most toxic fraction was found tobe the fraction 1 (LC50 31,62 ppm), but the fraction that was analysed further was fraction 3 (LC50 100 ppm),because fraction 1 consists of using compounds that were difficult to separate. The purity fraction 3 was testedconducted thin layer chromatography and its activity as antitumor agent was tested using Agrobacterium tumefacienA-208. The test was in 6 weeks and that fraction 3 has a potency as an antitumor agent at 1000 ppm.The identification with gas chromatography – mass spectroscopy indicate that the antitumor isolate frombitter melon contains 3 mayor compoundsnamely dioxtyl hexadioate esther, palmitic acid, stearic acid.

ISOLASI DAN IDENTIFIKASI SENYAWA GOLONGAN FLAVONOID PADA DAUN NANGKA (Artocarpus heterophyllus Lmk) DAN AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI Staphylococcus aureus Anak Agung Sagung Krisna Darmawati; I Gusti Agung Gede Bawa; I Wayan Suirta
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolation, identification and antibacterial activity of flavonoid compounds from jackfruit leaf (Artocarpus heterophyllus Lmk) extract have been done in this research. Extraction of 500 g dry powder of jackfruit leaf using maceration method produced 31.20 g concentrated brownish green ethanol extract. Partition of 30.00 g the ethanol extract gained three concentrated extracts in n-hexane (4.46 g), chloroform (0.84 g) and n-buthanol (6.65 g) fractions. The phytochemical test showed that n-buthanol extract contained flavonoid compound which was active as antibacterial agent with 15.75 mm inhibition capacity. Column chromatography analysis on n-buthanol extract with mobile phase n-hexana: ethyl acetate: n-butanol (8:2:1) gave 8 fractions (FA, FB, FC, FD, FE, FF, FG, FH). Phytochemical testing showed that fraction FA and fraction FH belong to flavonoide compounds. Analysis of FA using UV-Visible gained 2 peaks at ? 323.40 nm (band I) and ? 285.60 nm (band II) which indicated the flavonoide groups of flavanone or dihydroflavonol. Analysis of FH using UV-Visible gained 2 peaks at ? 323.40 nm (band I) and ? 285.60 nm (band II) suggested the flavonoide groups of flavone or flavonol (3-OH substitution).` By using shiffting reagent the isolate FA was suggested to contain dihydroflavonol group and FH was suggested to contain flavone with hydroxyl groups at C-2’, C-4’.C-5’, C-6’, C-6, C-7, and C-8. Infrared analysis showed that the isolate had –OH, C=O, C-O, C=C aromatic, CH aromatic, and CH aliphatic groups. Antibacterial testing of flavonoid compounds of fraction A and fraction H on Staphylococcus aureus with a concentration of 10.000 ppm inhibited 10.50 mm for fraction A and 7.25 mm for fraction H.

PENGARUH KONSENTRASI ASAM FOSFAT DAN BERAT SEMEN PUTIH SEBAGAI ADSORBEN DALAM PEMURNIAN CRUDE GLISEROL Lestari Lestari; Made Arsa; I Wayan Suirta
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The research of purification process of crude glycerol using phosphoric acid and white cement as adsorbent has been carried out. The aim of this research was to study the effect of phosphoric acid concentration and weight of white cement used as adsorbent in the purification process to the purity of glycerol. The research was done by the addition phosphoric acid in crude glycerol followed by adsorption with white cement. Glycerol quality parameters were determined by assesment of glycerol density, viscosity, and by comparing its GC-MS spectra to GC-MS spectra of standard glycerol. The result showed that purification process of glycerol using 5 % phosphoric acid and 5 grams of white cement gave the highest level of glycerol which was 84.20%. The density of purified glycerol obtained in this research was 1.2595 ± 3.1622 x 10-5 g/mL, the viscosity was 1.4372 ± 3.8333 x 10-3 Pa.s, and the GC_MS spectra purified glycerol showed similar fragmentation pattern and retention time with the glycerol standard.

Co-Authors Ali Sabikin Anak Agung Sagung Krisna Darmawati Dewa Ken Budiputra Dewi Novita Hardaningsih E. P. Siregar E. Sahara I Gede Tangkas Mei Yasa I Gusti Agung Gede Bawa I Gusti Ayu Kunti Sri Panca Dewi I K. G. H. Subawa I Made Dira Swantara I Made Oka Adi Parwata I Putu Eka Suwita Hegara I Wayan Sumarjaya I. A. Gede Widihati I. A. R. A. Asih I. A. R. Astiti Asih I.A.R.A. Asih Ida Bagus Putra Manuaba Kadek Agus Andika Pratama Ketut Gede Dharma Putra Ketut Ratnayani Komang Ardipa Saputra Lestari Lestari Made Arsa Minanti Arna Ekawati N. K. Gumiati N. W. Bogoriani Nasution, Dina Munawaroh Ni Komang Ariati Ni Luh Putu Kartika Mardiani Ni Luh Putu Mega Wahyuni Ni Luh Putu Putri Setianingsih Ni Luh Rustini Ni Made Puspawati P. A. Damayanti Putu Prisanti Putri Utami Putu Suarya S.R. Santi Saeful Bahri Sri Rahayu Santi Taka Iqbal Prakasa W.S. Rita WAHYU DWIDJANI S Wasudewa, Kresna Murti WIWIK SUSANAH RITA

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