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All Journal Jurnal Penelitian Pascapanen Pertanian Jurnal Rekayasa Bahan Alam dan Energi Berkelanjutan
Haryo Tejo Prakoso
Pusat Penelitian Bioteknologi dan Bioindustri Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Energy Environmental Science

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EXTRACTION, CHARACTERIZATION AND APPLICATION OF CHITOSAN DERIVED FROM MAGGOT (BLACK SOLDIER FLY) WASTE AS EDIBLE COATING FOR RED GRAPES (Vitis vinifera) Sri Wahyuni; Ranti Selvina; Puspa Julistia Puspita; Haryo Tejo Prakoso; Priyono priyono; Siswanto siswanto
Jurnal Penelitian Pascapanen Pertanian Vol 18, No 1 (2021): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v18n1.2021.45-56

Abstract

Black Soldier Fly (BSF) farming showed an increasing trend in the past 5 years. The production process generates a leftover, that is the exuviae which are still untapped. BSF exuviae contain chitin and chitosan which can be utilized in various fields, one of which is fruit preservatives. Grapes (Vitis vinifera) is known to have many benefits and has a content of vitamins A, B, C, and E but it has a short shelf life. The purpose of this study was to characterize chitosan from BSF exuviae and choose the best edible coating method to be applied to grapes to prolong the grape’s storage time. Characterization includes physical condition, percent solubility, degree of deacetylation, and yield of the chitosan. Then, edible coating tests were carried out with 2% acetic acid solvent followed by two different methods, namely dyeing method with 5, 10 and, 15 minutes immersion time and spray method with a chitosan solution concentration of 0,5%, 1%, 1,5%, 2 %, and 2,5%. The results showed that chitosan extracted from BSF exuviae produced a physical appearance in the form of white brown powder with 55% solubility and a degree of deacetylation of 91,88%. The chitin yield is 16,2%, followed by the chitosan yield of 4,8%. The best edible coating method which can maintain the physical appearance of grapes and inhibiting fruit decayed was obtained by dipping method at a concentration of 2,5% for 10 minutes. Based on the overall observations, however, the results obtained from the dip and spray method were not significantly different.
Synthesis of Chitosan Composite based on Black Soldier Fly (BSF) Exuviae with Kipahit Leaf Extract and its Inhibition Test against Xanthomonas Oryzae. Sri Wahyuni; Ridha Fauziyah; Muhammad Abdul Aziz; Deden Dewantara Eris; Haryo Tejo Prakoso; Siswanto siswanto; Priyono Priyono
Jurnal Rekayasa Bahan Alam dan Energi Berkelanjutan Vol. 5 No. 2 (2021)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rbaet.2021.005.02.03

Abstract

HDB disease caused by the bacteria Xanthomonas oryzae pv.oryzae (Xoo) is an important disease in rice plants. Recently, many organic based antibacterial agents are being developed, one of which is chitosan. One of the potential raw materials for producing chitosan is Black Soldier Fly (BSF) exuviae. During the development, chitosan was formulated with other ingredients such as kipahit leaves (Tithonia diversifolia). The purpose of this study is to synthesize BSF-based chitosan, formulate BSF-based chitosan composites with kipahit leaf extract using 0.1% sodium tripolyphosphate and test the antibacterial activity against Xanthomonas oryzae composites. The process of optimizing the extraction of kipahit leaves was carried out with a variety of solvents (2% acetic acid, 96% ethanol and 20% DMSO). The antibacterial activity test was carried out by the disc diffusion method at a concentration of 0.1; 0.2; 0.4; 0.6; 0.8 and 1% (w / v). The results showed that the best kipahit leaf extraction was obtained using 96% ethanol as a solvent. 
Co-Authors Deden Dewantara ERIS Muhammad Abdul Aziz Priyono priyono Puspa Julistia Puspita Ranti Selvina Ridha Fauziyah Siswanto siswanto Sri Wahyuni