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N. Wicaksana
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MOLECULAR CHARACTERIZATION OF QUALITY PROTEIN MAIZE AND DOWNY MILDEW RESISTANCE LINES BASED ON SIMPLE SEQUENCE REPEATS (SSRs) MARKER D. Ruswandi; N. Wicaksana; M. B. Pabendon; M. Azrai; M. Rachmadi; A. Ismail; N. Carsono; F. Damayanti; F. Kasim
Zuriat Vol 16, No 1 (2005)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v16i1.6779

Abstract

The information on germplasm diversity and genetic relatedness among elite breeding materials is an important element in maize breeding. Molecular characterization and genetic relationship of 11 QPM-DMR lines were analysed using thirty three SSRs markers. Genetic relationship was determined using Jaccard’s similarity coefficient, and dendogram was then constructed based on the unweighted pair-group method with arithmetical averages (UPGMA). Result showed that (i) all SSRs loci were informative for describing the genotypic variation as showed by their PIC, which ranged from 0.19 for umc1304 to 0.93 for phi112; (ii) the eleven maize inbred lines were clustered into one major group A and small groups B and C that corresponds well with the breeding programs adopted at different institutes of release, and (iii) thus, SSRs marker system is a valuable marker for varietals identification and for genetic diversity study of elite breeding materials.
HERITABILITY AND HETEROSIS OF GRAIN YIELD ON DOWNY MILDEW RESISTANCE (DMR) AND QUALITY PROTEIN MAIZE (QPM) INBREDS AND THEIR SINGLE CROSS HYBRIDS D. Ruswandi; N. Wicaksana; M. Rachmadi; A. Ismail; D. Arief; F. Kasim
Zuriat Vol 16, No 1 (2005)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v16i1.6780

Abstract

Improvement of high yield maize cultivars possessing high quality of seed as showed by high lysine and tryptophan content must be fulfilled with resistance against downy mildew pathogen in any maize breeding program. A set of experiment to study genetic variability and to estimate both heritability and heterosis of important characters of grain yield on DMR and QPM lines has been conducted. Four downy mildew resistance (DMR) lines, i.e. Nei 9008, P 345, Ki 3, and MR 10; three quality protein maize (QPM) lines, including CML 161, CML 163, and CML 172; and their single cross hybrids formed based on line × tester mating design were used in the experiment. The following characters: height of the first ear, seeds number per row, seed weight per ear, and grain yield per plot showed broad genetic variability. Narrow sense heritability (hns) of grain yield and its components ranged from 0.01 to 0.71. Broad sense heritability (hbs), on the other hand, ranged from 0.20 to 0.74. It was found that hybrid derived from crossing between parental inbred lines possessing broad genetic background would express high heterosis.
Co-Authors A. Ismail D. Arief D. Ruswandi F. Damayanti F. Kasim M. Azrai M. B. Pabendon M. Rachmadi N. Carsono