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All Journal Jurnal Sain Veteriner Jurnal Veteriner Samakia: Jurnal Ilmu Perikanan

Nisa Hakimah

Politeknik Kelautan Dan Perikanan Sidoarjo

Author-ID : 273048

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary Other

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Deteksi Kejadian Residu Tetrasiklin pada Daging Ikan Nila di Kota Yogyakarta dengan Kromatografi Cair Kinerja Tinggi (KCKT) Wari Pawestri; Gagak Dhony Satria; Nisa Hakimah; Doddi Yudhabuntara
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Food products of animal origin which are free from biological and chemical contamination are an absolute requirement of food safety. Antibiotics residues in the food of animal origin is one of the chemical contaminants in food are harmful/hazardous to human health. Tetracycline is an antibiotic that is often used in the fishing industry. The study aims to detect the occurrence of tetracycline residues in tilapia sold in traditional markets in Kota Yogyakarta. The research was conducted with detection test (detect disease). The study used 61 samples of tilapia fish from 16 traditional markets in Kota Yogyakarta. Tilapia meat samples are prepared according to the Association of Official Analytical Chemistry (AOAC). The existence of tetracycline residues in meat can be detected by high performance liquid chromatography (HPLC). Residue analysis in fish meat was conducted at the Department of Pharmacology, Faculty of Veterinary Medicine of Gadjah Mada University using HPLC Shimadzu version 6.1. The results showed that 24 samples of tilapia meat marketed in Kota Yogyakarta was positively contained tetracycline antibiotic residues. A total of 19 samples contained residues above maximum residue limits (MRL). The conclusion of the study is 31% of tilapia marketed in Kota Yogyakarta contains tetracycline residues above MRL. Surveillance of residues in food of animal origin and further research in terms of reducing antibiotic residue in tilapia meat is highly recommended to improve the quality and safety of food of animal origin.

Validasi Metode Analisis Tetrasiklin pada Ikan Nila (Oreochromis sp.) menggunakan Alat Kromatografi Cair Kinerja Tinggi (KCKT) Nisa Hakimah; Gagak Donny Satria; Wari Pawestri; Soedarmanto Indarjulianto
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Antibiotics are substances that capable of inhibiting the growth of or killing microorganisms. The presence of residues in the tissues are associated with continuous use of antibiotics for long periods of time. The antibiotic residue that is often detected in freshwater fishery products is tetracycline. One method of analysis of tetracycline residues in fish meat is widely developed using high performance liquid chromatography (HPLC). This research was aimed to validate the method of analysis of tetracycline content in tilapia meat by using HPLC Shimadzu 6.1. The mobile phase consisting of methanol: acetonitrile: oxalic acid (5:15:80) with 1 ml / min flow rate, detector UV Vis with wavelength 355 nm, and C18Shim-pack column size 150 L x 4,6 mm at temperature of 30oC. The result of this research showed values corresponding to validation criteria based on parameters of specificity, precision, accuracy, linearity, limit of detection, and limit of quantification. Tetracycline analysis using HPLC tool has good and acurate validity as the first step in detecting the tetracycline level in tilapia meat.

Residu Deltamethrin Terlacak pada Hati Ayam Pedaging yang Dipasarkan di Yogyakarta (DETECTION OF DELTAMETHRIN RESIDUE IN BROILER LIVER TRADED IN YOGYAKARTA) Gagak Donny Satria; Agustina Dwi Wijayanti; Puspa Wikan Sari; Dwi Cahyo Budi Setiawan; Nisa Hakimah; Annisa Rahmawati
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The consumer assurance to get safe, hygienic, whole, and halal food is an important concern. Analysisof chemical residue is one of some ways to examine food safety. The objective of this research was to detectdeltamethrin residue in broiler liver sold in Yogyakarta, Indonesia. The isocratic HPLC method was usedin this research, using Shimadzu 6.1, 80% of Acetonitrile in Aquabides as mobile phase, with 1ml/minutesof flow rate. C-18 was used as column and the wavelength of UV-Vis detector was 270 nm. The result of theresearch was get from HPLC’s analyses. Deltamethrin’s peak area would be presented in retention time 8-12minutes with specific profile of curve. The result of the research showed that deltamethrin was positivelydetected in 13 broiler livers. It’s concluded that broiler livers that sold in Yogyakarta are detected to containdeltamethrin-contaminated.

Deteksi Residu Oksitetrasiklin pada Ikan Lele yang Dipasarkan di Kota Yogyakarta Nisa Hakimah; Wari Pawestri; Dewi Nurmalita Suseno; Sri Widowati Anjarsari
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Tingkat konsumsi ikan di Indonesia terus mengalami peningkatan seiring dengan kesadaran masyarakat terhadap pemenuhan gizi dan meningkatnya pengetahuan tentang manfaat makan ikan bagi tubuh. Kebutuhan ini harus diimbangi dengan peningkatan kuantitas dan kualitas produk ikan. Salah satu kontaminan yang dapat menimbulkan permasalahan adalah cemaran antibiotik. Oksitetrasiklin merupakan antibiotik yang banyak digunakan pada dunia perikanan. Penelitian bertujuan untuk melakukan validasi metode kromatografi cair kinerja tinggi (KCKT) untuk analisis residu oksitetrasiklin dalam daging ikan lele dan melakukan deteksi kejadian residu oksitetrasiklin pada daging ikan lele yang dipasarkan di wilayah Kota Yogyakarta. Penelitian dilakukan dengan dua tahap yaitu tahap validasi dan tahap penelitian lapangan. Validasi metode preparasi sampel daging ikan lele menggunakan alat KCKT dan meliputi parameter spesifisitas, presisi, akurasi,dan linearitas. Hasil penelitian menunjukkan bahwa alat KCKT yang digunakan dalam penelitian dapat digunakan untuk melakukan deteksi residu oksitetrasiklin dalam daging ikan lele dengan baik. Penelitian lapangan menggunakan 59 sampel ikan lele yang diambil dari penjual yang berbeda di 16 pasar tradisional wilayah Kota Yogyakarta. Hasil penelitian menunjukkan adanya residu oksitetrasiklin, ditunjukkan dengan hasil positif (melampaui batas maksimum residu) pada 25% dari keseluruhan sampel.

Analisis Kadar Histamin pada Produk Olahan Ikan Pindang di Kabupaten Sidoarjo, Jawa Timur Menggunakan ELISA Budi Rianto Wahidi; Adi Suseno; Dewi Alima Nostalia Suseno; Dewi Nurmalita Suseno; Nisa Hakimah
Samakia : Jurnal Ilmu Perikanan Vol 13 No 2 (2022): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Histamin merupakan salah satu indikator keamanan mutu pangan untuk produk olahan ikan. Histamin dimungkinkan terkandung pada ikan pindang karena beberapa faktor. Penelitian ini bertujuan untuk mengetahui kadar histamin pada ikan pindang yang dijual dan dikonsumsi oleh masyarakat serta faktor-faktor yang mempengaruhi terbentuknya dan kenaikan kadar histamin pada ikan pindang. Sampel ikan pindang diperoleh dari beberapa pasar tradisional di Sidoarjo dan diuji menggunakan metode ELISA. Hasil uji terhadap beberapa parameter menujukkan bahwa nilai pH berkisar antara 6,05 – 6,95; kadar air 13,11 – 27,79%; kadar garam 6,71 – 12,31%; dan kadar histamin 4,143 – 11,450 ppm. Kadar histamin tertinggi diperoleh dari sampel ikan pindang dengan kadar air yang tinggi dan kadar garam yang rendah.

Co-Authors Adi Suseno Agustina Dwi Wijayanti ANNISA RAHMAWATI Budi Rianto Wahidi Dewi Alima Nostalia Suseno Dewi Nurmalita Suseno Dewi Nurmalita Suseno Doddi Yudhabuntara Dwi Cahyo Budi Setiawan Gagak Dhony Satria Gagak Donny Satria Gagak Donny Satria Puspa Wikan Sari Soedarmanto Indarjulianto Sri Widowati Anjarsari Wari Pawestri Wari Pawestri Wari Pawestri

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