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All Journal Jurnal Sain Veteriner Jurnal Akuakultur Rawa Indonesia Samakia: Jurnal Ilmu Perikanan
Budi Rianto Wahidi
Politeknik Kelautan Dan Perikanan Sidoarjo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary Other

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Phylogenetic Analysis of Thymidin Kinase Gene Koi HerpesVirus (KHV) in Some Freshwater Fish Aquaculture Centers in East Java Budi Rianto Wahidi
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28526.374 KB) | DOI: 10.22146/jsv.5430

Abstract

Koi Herpesvirus ( KHV ) is a virus that infects goldfish and koi that resulted in mass death and cause economic and social losses. The use of Polymerase Chain Reaction (PCR) technique to detect KHV was done  in nila and gurame. Proof that KHV could infect nila and gurame KHV following genetic variation in each definitive host and spreading area have not been done. Based on these problems, this research was conducted to study the genetic variability and genetic relationships between KHV isolates in some freshwater fish as the definitive host koi, komet , koki, nila and gurame that exist in the area of East Java. The results showed that clinically the gills were pale and hemorrage the end of the tail fin. Electrophoresis results showed that all samples from  KHV infected  koi, koki, komet, nila and gurame  were all  positive but the sequencing results for tilapia and carp could not be further identified using the BLAST program. Genetically,  koi fish, koki and komet isolates were identical to GenBank isolates code KHV-GZ11 and Indo_0K02SS.
Analisis Kadar Histamin pada Produk Olahan Ikan Pindang di Kabupaten Sidoarjo, Jawa Timur Menggunakan ELISA Budi Rianto Wahidi; Adi Suseno; Dewi Alima Nostalia Suseno; Dewi Nurmalita Suseno; Nisa Hakimah
Samakia : Jurnal Ilmu Perikanan Vol 13 No 2 (2022): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.523 KB) | DOI: 10.35316/jsapi.v13i2.1791

Abstract

Histamin merupakan salah satu indikator keamanan mutu pangan untuk produk olahan ikan. Histamin dimungkinkan terkandung pada ikan pindang karena beberapa faktor. Penelitian ini bertujuan untuk mengetahui kadar histamin pada ikan pindang yang dijual dan dikonsumsi oleh masyarakat serta faktor-faktor yang mempengaruhi terbentuknya dan kenaikan kadar histamin pada ikan pindang. Sampel ikan pindang diperoleh dari beberapa pasar tradisional di Sidoarjo dan diuji menggunakan metode ELISA. Hasil uji terhadap beberapa parameter menujukkan bahwa nilai pH berkisar antara 6,05 – 6,95; kadar air 13,11 – 27,79%; kadar garam 6,71 – 12,31%; dan kadar histamin 4,143 – 11,450 ppm. Kadar histamin tertinggi diperoleh dari sampel ikan pindang dengan kadar air yang tinggi dan kadar garam yang rendah.
GAMBARAN HISTOPATOLOGI Clarias gariepinus YANG TERINFEKSI Edwardsiella tarda DI KEDIRI - JAWA TIMUR Budi Rianto Wahidi; Novia Christi Prihartini
Jurnal Akuakultur Rawa Indonesia VOL 10, NO 2 (2022): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v10i2.18863

Abstract

Clarias gariepinus is one of the freshwater fish that is easy to culture, but is also susceptible to Edwardsiella tarda infection. Although this is convenient to treated, the consequences of this bacterial infection are quite detrimental. The method used in this study is a biochemical test followed by histopathological examination to observe tissue changes that occur in the Clarias gariepinus organ. The results showed that 5 out of 17 samples were infected with Edwardsiella tarda with pathological features showing edema, congestion, lymphocyte infiltration, macrophage proliferation, and necrosis in all organs.
Co-Authors Adi Suseno Dewi Alima Nostalia Suseno Dewi Nurmalita Suseno Nisa Hakimah Novia Christi Prihartini