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All Journal Jurnal Kedokteran Brawijaya Biomedical Journal of Indonesia
Gery Rifano Hardanto
Mahasiswa program pendidikan S-1 kedokteran umum Fakultas Kedokteran Universitas Diponegoro
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Effect of Artemisia vulgaris Extract for Increasing IL-12 and Granzyme Expression (Study on Mammary Adenocarcinoma C3H Mice Given Adriamycin- Cyclophosphamide Chemotherapy Regimen) Hardanto, Gery Rifano; Selamat Budijitno
Biomedical Journal of Indonesia Vol. 7 No. 3 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i3.539

Abstract

Background: The incidence of breast cancer worldwide is still high. Surgery remains the top choice with other modalities of chemotherapy, radiation, and immunotherapy such as Artemisia vulgaris (AV). The study was aimed to demonstrate that administration of AV extract increased p53 expression and the apoptotic index in mammary adenocarcinoma. Methods: This study used a "Post-test only control group design" on 24 females C3H mice that were randomly selected and divided into four groups: group K (control), P1 (chemotherapy), P2 (extract), and P3 (combination). Mammary adenocarcinoma comes from the inoculation of donor mice. Chemotherapy of Adriamycin 0,18 mg and Cyclophosphamide 1,8 mg were given in 2 cycles. AV 13 mg (0.2 ml) was given once daily orally. IL-12 and granzyme expression were evaluated by immunohistochemical staining. Results : Mean of IL-12 and Granzyme expression were found in groups K, P1, P2, P3 were 50,40 ± 1,56, 60,28 ± 1,54, 53,48 ± 1,35, 75,40 ± 1,46 dan 14,96 ± 0,61, 24,86 ± 1,21, 17,14 ± 1,02, 26,62 ± 0,70. The statistical analysis showed that there were significant differences in the IL-12 between groups of K vs P1 (0,001), P1 vs P2 (0,001), K vs P2 (0,028), P1 vs P3 (0,001), K vs P3 (0,001), P2 vs P3 (0,001) and in granzyme expression between groups of K vs P1 (0,001), P1 vs P2 (0,001), K vs P2 (0,010), P1 vs P3 (0,047), K vs P3 (0,001), P2 vs P3 (0,001). Correlation analysis between IL-12 and Granzyme expression were found significant correlation (p = 0,001 and r = -0,911). Conclusion: Artemisia vulgaris can improve the effectivity of Adriamycin-Cyclophosphamide chemotherapy on C3H mice with mammary adenocarcinoma in terms of elevated IL-12 and Granzyme expression.
Effect of Artemisia vulgaris Extract on Granzyme Expression and Tumor Mass Diameter (Study of Adriamycin Cyclophosphamide Chemotherapy in Adenocarcinoma Mammae C3H Mice Model) Hardanto, Gery Rifano; Budijitno, Selamat; Hardian, Hardian
Jurnal Kedokteran Brawijaya Vol 31, No 4 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.04.1

Abstract

Breast cancer incident continues to increase globally. The surgical management can be combined with other therapeutic modalities, including chemotherapy, radiation, and immunotherapy, such as Artemisia vulgaris (AV). This study aimed to determine the effect of AV extract on Granzyme expression and tumor mass diameter growth of C3H mice with adenocarcinoma mammae. Twenty-four female C3H mice were randomly divided into groups of K (control), P1 (AC chemotherapy), P2 (AV extract), and P3 (combination). Adenocarcinoma mammae were inoculated from donor mice. Two cycles of chemotherapy by Adriamycin 0.18 mg and Cyclophosphamide 1.8 mg were given intravenously, while Artemisia vulgaris 13 mg (0.2 ml) was given orally once per day. Granzyme expression was assessed using immunohistochemical staining, while tumor mass diameter growth was measured using tumor calipers. There was a significant negative correlation between and tumor mass diameter growth (p=0,001 and r=-0,911). Artemisia vulgaris increases the apoptotic effects of Adriamycin-Cyclophosphamide chemotherapy by increasing Granzyme expression and decreasing tumor mass diameter growth in adenocarcinoma mammae C3H mice.
Co-Authors Hardian Hardian Selamat Budijitno Selamat Budijitno