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All Journal JURNAL PEMULIAAN TANAMAN HUTAN Indonesian Journal of Biotechnology
Eko Bhakti Hardiyanto
Fakultas Kehutanan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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Improvement of Seed Orchard Management Based on Mating System of Cajuputi Trees Kartikawati, Noor Khomsah; Naiem, Mohammad; Hardiyanto, Eko Bhakti; Rimbawanto, Anto
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Breeding plan of cajuputi in Indonesia is aimed to increase plantation productivity of oil yield and 1.8 cineole content. Seed orchard of cajuputi at Paliyan, Gunungkidul, established using selected and genetically improved materials, has been producing seeds for operational plantation. This seed orchard would perform optimally if the mating systems of all individuals contribute to the inheritance of all genetic potential of the offsprings. Therefore, investigation of the mating systems of cajuputi was indispensible. The study has been carried out on 10 selected mother trees and the 24 offsprings of each mother trees using 8 microsatellite markers of nuclear DNA, namely Hin-2 (100-132 bp), Hin-4 (79-114 bp), Hin-5 (128-148 bp), Hin-7 (136-224 bp), Sal-1 (93-99 bp), Sal-3 (118-219 bp), Xho-1 (96-111 bp) and Xho-4 (150-216 bp), respectively. The result showed relatively high genetic variation of the offspring (HE=0.602, HO=0.594) originated from parent trees in the seed orchard. Parent trees tend to outcross(tm=0.951, ts=0.806), although seeds originated from biparental inbred (tm – ts = 0.145) and correlated paternity(rp=0.098) have also been observed. This genetically viable population could maintain its reproduction fi tness forshort term and adapt to the dynamic environmental changes for long term.Key words: mating system, cajuputi, seed orchard, microsatellite
Estimasi parameter genetik dan peran gen pada uji keturunan full sib kayu putih di Gunung Kidul Kartikawati, Noor Khomsah; Naiem, Mohammad; Hardiyanto, Eko Bhakti
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 1 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : BBPPBPTH

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Controlled pollination technique could be applied to generate gene recombinant from families in progeny test and to estimate genetic parameters. The early step in evaluation of controlled pollination is to estimate general combining ability and specific combining ability. the objectives of this research were to estimate generic parameters on full sib progeny test of melaleuca cajuputi subsp cajuputi cajuput and to identify gene action controlling growth and oil traits. The research was conducted on full sib progeny test at Gunungkidul established in an incomplete block Design with six treeplots and eight replications. Tree height and diameter stem growth were observed at seven years old. Oil yield and cineol content were examined using gas chromatography. Analysis of variance and genetic parameter were calculated for all measured variables. The result showed that individual heretabilities on growth traits (hi2 of stem diameter = 0,34 and tree height = 0,01, respectively) were lower than on oil traiths (hi2 of oil yield =0,6 and cineole = 0,4 respectively). This indicated that oil traits tend to genetically controlled. Analysis of variances on general combining ability (GCA) and Specific Combining Ability (SCA) effects from a serries of 15 sets of 5x5 half-diallel mating experiments showed that all traits (height, diameter, oil and 1,8 cineole yields) were controlled by non-aditive genes. Consequently, controlled pollination of selected plus trees should be carried out until heterosis of improved progenies could be found.
Co-Authors Anto Rimbawanto Mohammad Naiem Mudji Susanto Noor Khomsah Kartikawati, Noor Khomsah T.A. Prayitno