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All Journal Jurnal Sain Veteriner Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology JURNAL KAJIAN VETERINER Jurnal Nasional Teknologi Terapan Jurnal Kedokteran Hewan
Aris Haryanto
Fakultas Kedokteran Hewan Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Sain Veteriner

 1 
PREPARATION OF LYMPHOCYTE CULTURE CELL FROM PERIPHERIAL BLOOD OF NASOPHARYNGEAL CARCINOMA PATIENTS Aris Haryanto; Sofia Mubarika; Nastiti Wijayanti
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8239

Abstract

Lymphocyte is leukocyte component that difficult to culture in vitro. Several viruses need lymphocytes as host cell in order to proliferate and growth in this media such as Epstein-Barr virus (EBV). This virus was associated with malignant disease like nasopharyngeal carcinoma (NPC). The objectives of this research are to develop and to prepare lymphocyte cell culture as material source of DNA for molecular analysis of virus. Peripheral blood was collected from NPC patients which is histopatologically and serologically positive of EBV. Lymphocytes were separated from the other blood components using ficcol-histopaque. Lymphocytes were diluted using RPMt medium then they were cultivated into 96 microwell plate with concentration of 106 cell/ml. The medium consist of 10% FBS, RPMI, Penstrep and FK 506. Culture of lymphocytes were incubated in 5% CO2 at 37°C. The lymphocyte cultures developed and grew confluendy during the first week. Only B cells whith EBV would be well establish. After 50 cell generations, lymphocytes were transformed and immortalized to be lymphoblastoid cell fine (LCL).
Co-Authors Charles Rangga Tabu Diana Savitri Diana Savitri Dini Wahyu Yudianingtyas Duhita Andinita Hevi Wihadmadyatami Ida Tjahajati Irhamna Putri Irhamna Putri Medania Purwaningrum Moenek, Devi Y.J.A. Muh. Disna Faizal Nastiti Wijayanti Nastiti Wijayanti Ratu Fresa Khoirotunnisa Hidayat Rini Widayanti Sofia Mubarika Sri Handayani Irianingsih Teuku Zahrial Helmi Teuku Zahrial Helmy Verawati Verawati Warih Pulung Nugrahani Warih Pulung Nugrahani