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All Journal Jurnal Penelitian Farmasi Indonesia Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Chimica et Natura Acta Scientific Journal of Reflection : Economic, Accounting, Management and Business Pharmacology and Clinical Pharmacy Research JURNAL PHOTON
Yuli Haryani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics

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Uji Parameter Non Spesifik dan Aktivitas Antibakteri Ekstrak Metanol dari Umbi Tanaman Dahlia (Dahlia variabilis) Haryani, Yuli; Muthmainah, Siti; Sikumbang, Saryono
Jurnal Penelitian Farmasi Indonesia Vol 1, No 2 (2013)
Publisher : Jurnal Penelitian Farmasi Indonesia

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Abstract

Dahlia variabilis is one of plant that commonly growing in highland hills in Indonesia. The preliminary screening on secondary metabolitesshowed that the presence of flavonoids, phenolics and saponin in methanol extract of this plant’s tuber. Its methanol extract was subjectedto the evaluation of non-specific parameter of extract standardization as well as antimicrobial assay using disc diffusion method againstEscherichia coli and Staphylococcus aureus. The result revealed that the methanol extract contained 25.48±1.63% of water content,4.06±0.15% of ash content, and there was no heavy metal contamination detected. Antibacterial activity assay of three differenceconcentrations of the extract (10; 8 and 2%) demonstrated that extract with 10% of concentration gave the strongest activity against twotested bacterium with diameter of inhibition of 8.67±0.57 and 8.33±0.28 mm against Escherichia coli and Staphylococcus aureus, respectively.
PEMANFAATAN EKSTRAK AIR RIMPANG JAHE MERAH (Zingiber officinale Linn. var. rubrum) PADA BIOSINTESIS SEDERHANA NANOPARTIKEL PERAK Haryani, Yuli; Kartika, Ganis Fia; Yuharmen, Yuharmen; Putri, Eka Marissa; Alchalish, Dhia Tijani; Melanie, Yonatha
Chimica et Natura Acta Vol 4, No 3 (2016)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.149 KB) | DOI: 10.24198/cna.v4.n3.10989

Abstract

Penelitian ini bertujuan untuk melakukan biosintesis nanopartikel perak menggunakan ekstrak rimpang jahe merah sebagai pereduksi pada suhu ruang. Sintesis dilakukan dengan pengadukan pada shaker (150 rpm) dan tanpa pengadukan dengan rasio ekstrak dan larutan AgNO3 1:1; 1:3; 1:4; 1:5; 1:10 dan 1:20 pada berbagai rentang waktu pengamatan. Hasil memperlihatkan bahwa metabolit sekunder pada ekstrak jahe merah mampu mereduksi Ag+ menjadi Ag0 dan membentuk nanopartikel perak yang diindikasikan dengan adanya perubahan warna larutan. Nanopartikel perak dikarakterisasi menggunakan spektrofotometer UV-Vis dan absorbansi maksimum terukur di sekitar 450 nm. Secara keseluruhan, sintesis dengan pengadukan menghasilkan produk dengan waktu yang lebih singkat dibanding tanpa pengadukan.
Phaleria macrocarpa (Boerl.) Scheff Fruit: A Potential Source of Natural Antioxidant Hendra, Rudi; Haryani, Yuli
Pharmacology and Clinical Pharmacy Research Vol 3, No 1
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.319 KB) | DOI: 10.15416/pcpr.v3i1.16448

Abstract

Phaleria macrocarpa (Scheff.) Boerl is originated from Papua Island, Indonesia. There have been only limited attempts to explore the biological properties of this plant in relation to their medicinal use. This study aimed to examine antioxidant activity of P. macrocarpa fruit. Extraction of pericarp and mesocarp of P. macrocarpa were performed using soxhlet method with ethyl acetate as the solvent. Antioxidant activity was characterized in various in vitro model systems, including DPPH and ferric reducing antioxidant assay. We found that the highest amount of phenolic compounds and flavonoids were found in the pericarp (58.3±0.07 mg/g DW and 127.8±1.08 mg/g DW, respectively). The results showed that pericarp had higher antioxidant activity (IC50= 122.4±1.14 µg/ml) compared to mesocarp (IC50=175.48 ±1.75 µg/ml). In conclusion, the result of this study indicated the possible application of P. macrocarpa as a source of natural antioxidant compound.Keywords: antioxidant, Phaleria macrocarpa, phenolic compounds, flavonoids
Synthesis of Silver Nanoparticles from Cucumis melo L. and Assessment of Its Antimicrobial Properties Haryani, Yuli; Nabella, Iswatun; Yuharmen, Yuharmen; Kartika, Ganis F.
Pharmacology and Clinical Pharmacy Research Vol 3, No 2 (2018)
Publisher : Universitas Padjadjaran, Indonesia

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Abstract

Over the past decade, the use of biological agents such as plants, cyanobacteria, bacteria, and fungi for synthesis of metal nanoparticles has been developed. The aim of this study was to investigate antibacterial activity of Cucumis melo L. peel extract and its nanoparticles formulation against Eschericia coli. The nanoparticles were made using silver nitrate with the ratio between C. melo L. extract and silver nitrate aqueous solution (1 mM) were 1:10 and 1:15. The formation of silver nanoparticles was observed after microwaved for 30, 60, 90, 120, 150, and 180 seconds by visible spectrophotometry analysis. Phytochemical screening revealed the presence of flavonoid and terpenoid within the extract. However, the characteristic of surface plasmon resonance band, which occurs in the range of 410-500 nm were not found in the nanoparticle extract, even though the reaction time was extended to 330s. Antibacterial activity against E. coli of the extract and its nanoparticle formulations was determined using Resazurin microtiter assay and compared to Amoxsan® as positive control. The highest E. coli inhibition was exhibited by the nanoparticles (79.8739±0.3859), followed by the extract (65.2821±0.9949). The nanoparticles and the extract have potent antibacterial activity compared to positive control (84.5519 ± 0.2544). In conclusion, the antibacterial activity of the C. melo L. silver nanoparticles formulation was better than its extract.Keywords: Cucumis melo, silver nanoparticles, UV-Vis spectra
UJI AKTIVITAS ENZIM PROTEASE DARI ISOLAT Bacillus sp. ENDOFIT TANAMAN KELAPA SAWIT (Elaeis quinensis) Marnolia, Atika; Haryani, Yuli; Puspita, Fifi
Sistem Informasi Vol 6 No 02 (2016): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Proteolytic bacteria are able to hydrolyze proteins into amino acid and smaller peptides. The objective of this study to determine the abilty of three Bacillus sp. isolated from leaf (BD), root (BA), and stem (BP) of palm oil to produce protease. The proteolytic activity was determine based on the amount of tyrosin liberated in Unit/mL, while the specific activity was indicated by activity per unit weight of proteins. Protein concentration of enzymes extracts was determined by Lowry method. The results showed that all isolates were able to produce protease. Protease activity of Bacillus sp. BD, BA and BP was (711,260 ± 0,735) x 10-3 U/mL, (708,536 ± 0,918) x 10-3 U/mL and (710,027 ± 0,091) x 10-3 U/mL in optimal production times of 24 hours, repectively.
UJI AKTIVITAS SELULOLITIK DARI TIGA ISOLAT BAKTERI Bacillus sp. GALUR LOKAL RIAU Rahayu, Ariani Gusti; Haryani, Yuli; Puspita, Fifi
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cellulolytic bacteria are bacteria that have ability to hydrolize cellulose into smaller oligosaccharides and into glucose units. Glucose is used as a source of carbon and energy for bacterial growth. The aims of this study wasto determine the ability of three bacillus sp. bacteria from rhizosphere of mustard plants (Bacillus sp. B1), rhizosphere of rice plants (Bacillus sp. B2), and peat soil of Giam Siak Kecil (Bacillus sp. B3) to produce cellulase enzymes based on clear zone which is seen surrounding the colonies. Cellulolitic activity of the bacteria was determined by their ability bacteria to degrade carboxymethyl cellulose (CMC) substrate. The results showed that all isolates tested were able to produce cellulase. Bacillus sp. B1 isolate had the highest cellulolitic activity [(6.502 ± 0.462) x 10 -3 U/mL] than 2 isolates based on 24 hourproduction time and120 rpmspeed of agitation.
TOTAL FENOLIK, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN DARI PRODUK TEH HIJAU DAN TEH HITAM TANAMAN BANGUN-BANGUN (Coleus amboinicus) DENGAN PERLAKUAN ETT RUMPUT PAITAN Rahayu, Fitri; Jose, Christine; Haryani, Yuli
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Coleus amboinicus is one plant with a good antioxidant content. The concentration of antioxidant from C. amboinicus could be increased by using a treatment fermented plant extract (FPE) containing paitan grass. C. amboinicus is potential to be used as raw material to produce herbal tea such as green tea and black tea because of its high antioxidant content. The objective of this study was to analyze the total phenolics, flavonoids, and antioxidant activity from tea product of C. amboinicus. Antioxidant activity was analyzed using one method, that Ferric Reducing Antioxidant Power (FRAP). The results showed that C. amboinicus treated with FPE paitan grass had the highest total phenolic, flavonoid, and antioxidant activity content than control. The green tea produced using FPE paitan grass had the highest content of total phenolic and flavonoid i.e., 25,596 Gallic Acid Equivalent (GAE) mg/g of dry weight (DW) and 5,632 Catechin Equivalent (CTE) mg/g of DW, respectively. Antioxidant activity analyzed by using FRAP method also showed that the highest antioxidant was found in green tea treated using FPE paitan grass. It can be concluded that green tea C. amboinicus treated by FPE paitan grass is a good source of phenolic, flavonoid, and antioxidant.
PENENTUAN TOTAL MIKROBA INDIKATOR, NITRAT, DAN FOSFAT PADA SUNGAI TAPUNG KIRI Rosidah '; Yuli Haryani; Ganis Fia Kartika
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The aim of this study was to analyze physicochemical parameters  and  total  microbial indicators  of river water  in Tapung Kiri river. Water samples were taken from  4 sampling sites in  July  2013.  The results showed that the range of  TDS,  turbidity,  electrical conductivity,  and nitrate in  the  water samples  were  9-21 mg/L, 3.89-41.1 NTU, 17.72-42.0 µs/cm,  and  0.112-0.508 mg/L, respectively which  were  below of  the threshold value. Temperature, pH, DO,  and  phosphate content range  were  27-29oC,  4.75-6.30,  3.36-3.96 mg/L, and 0.089-0.235 mg/L at the threshold value based on PP No 82 2001. The level of Coliform  and  E. coli  contamination in  the  water samples were  0.4-18×10 3 CFU/mL and 0.1-4×10 3 CFU/mL.  The level of total bacteria contamination was found in all samples ranged from  0.43-4.6×10 4 MPN/mL, while the fungi  contamination was  0.1-0.3×10 3 CFU/mL.
PREVALENSI BAKTERI COLIFORM DAN Escherichia coli PADA DAGING SAPI YANG DIJUAL DI PASAR TRADISIONAL DAN PASAR MODERN DI KOTA PEKANBARU Jasmadi '; Yuli Haryani; Christine Jose
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Beef is potential as a medium for microbial growth such as coliform bacteria since it has a high nutrients content. Coliform bacteria in a given amount could be used as a hygienic indicator and a sign for the presence of pathogenic bacteria. One species of coliform bacteria that often contaminate meat is Escherichia coli. Escherichia coli is a normal flora in gastrointestinal tract, but some strains of them are pathogens which cause acute diarrhea. The aim of this study was to calculate the number of coliformbacteria and to detect the presence of Escherichia coli in beef sold in traditional and modern markets in Pekanbaru using the Most Probable Number method. The results showed that all samples were found to be contaminated by Escherichia coli andcoliform bacteria. The number of coliform bacteria in all samples exceeded the maximum threshold of bacterial contamination according to Indonesian National Standard No. 7388:2009, i.e 1x102 MPN/g. The number of coliform bacteria in samplessold at traditional and modern markets were in the range of 0.11 x10 7 MPN/g to >24x10 7 MPN/g and 0.35 x10 8 MPN/g to >24x10 8 MPN/g, respectively.
JUMLAH BAKTERI COLIFORM DAN DETEKSI Escherichia coli PADA DAGING AYAM DI PEKANBARU Usna Juwita; Yuli Haryani; Christine Jose
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Coliform bacteria are often referred as indicator organisms to indicate the potential presence of disease-causing bacteria. Escherichia coli is a type of Coliform bacteria. Most Escherichia coli are harmless and commonly found in the intestines of humans and animals. However, some strains can cause illness. Coliform bacteria and Escherichia coli contamination in chicken could be detected using Most Probable Number (MPN) multi-step assay which consists of presumptive, confirmed, and completed test. The aim of this study was to determine the number of Coliform bacteria contamination and to detect Escherichia coli in 15 chicken meat samples collected from 3 traditional markets and 3 modern markets in Pekanbaru. Estimated numbers ofColiform bacteria in samples were varied from 2.1x10 4 MPN/g to >11x10 MPN/g. We found that chicken samples were contaminated by Escherichia coli, which showed colour changes and bubles production in EC broth and the appearence of dark centered colony with metallic shine on EMB Agar.
Co-Authors Alchalish, Dhia Tijani Ariani Gusti Rahayu Christine Jose Dian Permata Sari Fifi Puspita Fitri Rahayu Franka Hendra Sukma Ganis Fia Kartika Jasmadi ' Jisman Hutasoit Kartika, Ganis F. Kiki Apriani Kurniawan Agung Marnolia, Atika Melanie, Yonatha Nabella, Iswatun Putri, Eka Marissa Retno Indriyati K Rosidah ' Rudi Hendra Rudi Hendra Sarlly Deviani Saryono Sikumbang Silvera Devi Siti Muthmainah Usna Juwita Yuharmen, Yuharmen Yuharmen, Yuharmen