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All Journal Jurnal Ilmu Lingkungan Jurnal Penelitian Farmasi Indonesia Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Panrita Abdi - Jurnal Pengabdian pada Masyarakat JURNAL PHOTON
Christine Jose
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Agriculture, Biological Sciences & Forestry Chemistry Decision Sciences, Operations Research & Management Environmental Science Mathematics Medicine & Pharmacology Nursing Physics Other

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Aktivitas Antioksidan dan Antimikrobial dari Ekstrak Plectranthus amboinicus Teruna, Hilwan Yuda; Jose, Christine
Jurnal Penelitian Farmasi Indonesia Vol 1, No 2 (2013)
Publisher : Jurnal Penelitian Farmasi Indonesia

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Abstract

Plectranthus amboinicus or bangun-bangun is a traditional medicinal plant used for postpartum recovery in North Sumatera. The aims ofthis study were to detemine antioxidant and antimicrobial activity of leaves Plectranthus amboinicus. The leaves of Plectranthus amboinicuswere extracted with using n-hexane, ethyl acetate and methanol, successively. The extracts were tested to antioxidant activity assay usingDPPH with two fold dillution tehnique and ascorbic acid was used as standard. The antimicrobial activity was conducted using agar diffusionmethod against Escherichia coli, Staphylococcus aureus and Candida albicans. The Amoxsan used as positive control for antibacterialassay, while ketokenazole was for antifungi test. Extracted solvents were used as negative control. The n-hexane and ethyl acetate extractsdid not show any antioxidant activity. While, the IC 50 free radical DPPH of methanol extract was 90.96 mg/mL and ascorbic acid was 58.792mg/mL. The antimicrobial activity of n-hexane and Amoxsan againts Escherichia coli were 11.95 and 7.53 mm. The inhibition zone againtsStaphylococcus aureus were 11.30 and 11.57 mm. The antimicrobial activity of ethyl acetate and Amoxsan againts Escherichia coli were12.29 and 7.71 mm. The inhibition zone against Staphylococcus aureus were 13.72 and 11.57 mm. The n-hexane and ethyl acetate did notshow any antifungi activity towards Candida albicans. The methanol extract did not show any inhibition zone againts the selectedpathogens.
AKTIVITAS ANTIRADIKAL BEBAS SENYAWA TURUNAN CALKON DAN TURUNAN BENZOTIAZEPIN -, Sulismayati; -, Jasril; Jose, Christine; Hilma, Rahmiwati
Sistem Informasi Vol 4 No 2 (2014): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Senyawa calkon merupakan flavonoid rantai terbuka dengan cincin aromatik yang dihubungkan oleh tiga atom karbon dengan system α, β karbonil keton tidak jenuh. Calkobn juga merupakan senyawa antara untuk membuat senyawa lain salah satunya adalah benzotiazepin. Benzotiazepin adalah senyawa heterosiklik yang mengandung nitrogen dan sulfur. Hasil uji aktivitas anti radikal bebas dari senyawa calkon Y1 dan senyawa benzotiazepin Bt1 memperlihatkan aktivitas yang sangat lemah, ditandai dengan nilai IC50 yang lebih besar dari 1000 ppm jika dibandingkan dengan standar yang digunakan yaitu vitamin C dengan nilai IC50 33,539 ppm. Hal ini mungkin disebabkan karena tidak adanya substituen ataupun gugus yang dapat menyumbangkan proton pada kedua senyawa hasil sintesis sehingga menyebabkan kedua senyawa tersebut tidak aktif sebagai antiradical bebas.
TOTAL FENOLIK, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN DARI PRODUK TEH HIJAU DAN TEH HITAM TANAMAN BANGUN-BANGUN (Coleus amboinicus) DENGAN PERLAKUAN ETT RUMPUT PAITAN Rahayu, Fitri; Jose, Christine; Haryani, Yuli
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Coleus amboinicus is one plant with a good antioxidant content. The concentration of antioxidant from C. amboinicus could be increased by using a treatment fermented plant extract (FPE) containing paitan grass. C. amboinicus is potential to be used as raw material to produce herbal tea such as green tea and black tea because of its high antioxidant content. The objective of this study was to analyze the total phenolics, flavonoids, and antioxidant activity from tea product of C. amboinicus. Antioxidant activity was analyzed using one method, that Ferric Reducing Antioxidant Power (FRAP). The results showed that C. amboinicus treated with FPE paitan grass had the highest total phenolic, flavonoid, and antioxidant activity content than control. The green tea produced using FPE paitan grass had the highest content of total phenolic and flavonoid i.e., 25,596 Gallic Acid Equivalent (GAE) mg/g of dry weight (DW) and 5,632 Catechin Equivalent (CTE) mg/g of DW, respectively. Antioxidant activity analyzed by using FRAP method also showed that the highest antioxidant was found in green tea treated using FPE paitan grass. It can be concluded that green tea C. amboinicus treated by FPE paitan grass is a good source of phenolic, flavonoid, and antioxidant.
ANALISIS KADAR RESIDU PESTISIDA ORGANOFOSFAT DAN ANTIOKSIDAN PADA BAYAM (AMARANTHUS SP) DIPERKEBUNAN KARTAMA KECAMATAN MARPOYAN DAMAI PEKANBARU. Riski Novera Yenita; Bintal Amin; Christine Jose
Jurnal Ilmu Lingkungan Vol 6, No 2 (2012): Jurnal Ilmu Lingkungan
Publisher : Program Pascasarjana Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jil.6.2.p.114-122

Abstract

This study has been conducted from May to June 2012 to analyze the levels oforganophosphate pesticide residues and antioxidant in two types of spinach taken fromKartama Plantation District of Marpoyan Damai Pekanbaru. The results showed thatorganophosphate pesticide residues in green spinach were higher than that in red spinach. On the contrery, the levels of antioxidant were found to be higher in green spinach than thered one. In general, there was no significant correlation between pesticide residues andantioxidant in both types of spinach. Nine out of eighteen analyzed samples were exceededthe permissible level of pesticide in commonly consumed vegetables.
TOTAL FENOLIK,FLAVONOID SERTA AKTIVITAS ANTIOKSIDAN EKSTRAK n-HEKSANA, DIKLOROMETAN DAN METANOL Amaranthus spinosus L EM5-BAWANG PUTIH Yondra Arif D; Christine Jose; Hilwan Yuda Teruna
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The objective of this study was to analyze the total phenolics, total flavonoids, andantioxidant activities of Amaranthus spinosus L leaves. During cultivation, these plantswere treated with 2 treatments, biocontrol Effective Microorganism 5 (EM5) garlic andcontrol (water). The A. spinosus L leaves were dried using freeze dryer and extracted bydissolving n-hexane, dicloromethane (DCM) and methanol. The results showed thatA.spinosus L leaves treated with biocontrol had the highest total phenolic, totalflavonoid and antioxidant activity content compare to the control. The total phenolic,total flavonoid, 2,2 diphenyl-1-picrylhydrazyl (DPPH) and Nitric Oxide (NO) radicalscavenging were the highest on DCM extract with each value of 74,656 mg Gallic AcidEquivalent (GAE)/g of dry weight (DW), 22,337 mg Catechin Equivalent (CE)/g DW,0,373 mg/mL and 0,025 mg/mL. n-Hexane extract had the highest antioxidant activityon Ferric Thiocyanate ( FTC) test (27,615 %), meanwhile methanol extract indicated thehighest antioxidant activity on FRAP test (1,022 mmol Fe +2 /g DW). From this study,it can be concluded that A. spinosus L leaves with biocontrol EffectiveMicroorganism 5 (EM5) garlic can increase the phenolic, flavonoids and antioxidantactivitiy contents of A. spinosus L plant.
PREVALENSI BAKTERI COLIFORM DAN Escherichia coli PADA DAGING SAPI YANG DIJUAL DI PASAR TRADISIONAL DAN PASAR MODERN DI KOTA PEKANBARU Jasmadi '; Yuli Haryani; Christine Jose
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Beef is potential as a medium for microbial growth such as coliform bacteria since it has a high nutrients content. Coliform bacteria in a given amount could be used as a hygienic indicator and a sign for the presence of pathogenic bacteria. One species of coliform bacteria that often contaminate meat is Escherichia coli. Escherichia coli is a normal flora in gastrointestinal tract, but some strains of them are pathogens which cause acute diarrhea. The aim of this study was to calculate the number of coliformbacteria and to detect the presence of Escherichia coli in beef sold in traditional and modern markets in Pekanbaru using the Most Probable Number method. The results showed that all samples were found to be contaminated by Escherichia coli andcoliform bacteria. The number of coliform bacteria in all samples exceeded the maximum threshold of bacterial contamination according to Indonesian National Standard No. 7388:2009, i.e 1x102 MPN/g. The number of coliform bacteria in samplessold at traditional and modern markets were in the range of 0.11 x10 7 MPN/g to >24x10 7 MPN/g and 0.35 x10 8 MPN/g to >24x10 8 MPN/g, respectively.
ISOLASI BAKTERI SELULOLITIK DARI PERAIRAN DUMAI Waidil Anuar; Andi Dahliaty; Christine Jose
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Dumai water samples are estimated to contain cellulolytic microorganisms because of farm waste. Samples 930 from Sei Pakning and 931 from Rupat Strait were water samples containing cellulolytic bacteria taken from Dumai. In this study the production of cellulase enzymes from cellulolytic bacteria in samples of 930 and 931 used CMC as a substrate. Cellulase is an enzyme that catalyses the hidrolisis of β-1-4-glycosidic bond of cellulose. All of the isolates produced cellulase and were able to degrade cellulose. The results indicated that 1% substrate gave the highest activity of Cellulase in the 931-4A (Rupat strait) (1.186 ± 0.319) x 10 -3 U/mL, whereas the highest specific enzyme activity of the bacterial isolates was 930-1C (8.438 ± 0.109) x 10 -3 U/mg.
JUMLAH BAKTERI COLIFORM DAN DETEKSI Escherichia coli PADA DAGING AYAM DI PEKANBARU Usna Juwita; Yuli Haryani; Christine Jose
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Coliform bacteria are often referred as indicator organisms to indicate the potential presence of disease-causing bacteria. Escherichia coli is a type of Coliform bacteria. Most Escherichia coli are harmless and commonly found in the intestines of humans and animals. However, some strains can cause illness. Coliform bacteria and Escherichia coli contamination in chicken could be detected using Most Probable Number (MPN) multi-step assay which consists of presumptive, confirmed, and completed test. The aim of this study was to determine the number of Coliform bacteria contamination and to detect Escherichia coli in 15 chicken meat samples collected from 3 traditional markets and 3 modern markets in Pekanbaru. Estimated numbers ofColiform bacteria in samples were varied from 2.1x10 4 MPN/g to >11x10 MPN/g. We found that chicken samples were contaminated by Escherichia coli, which showed colour changes and bubles production in EC broth and the appearence of dark centered colony with metallic shine on EMB Agar.
ISOLASI DAN UJI AKTIVITAS BAKTERI SELULOLITIK DARI AIR MUARA DAERAH ALIRAN SUNGAI SIAK WILAYAH KABUPATEN BENGKALIS Sarlly Deviani; Yuli Haryani; Christine Jose
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cellulolytic bacteria are  bacteria that can degrade cellulose into smaller polysaccharides called cellodextrins or completely into glucose units. Glucose is used as a carbon source for bacterial growth. The aims  of this study were to isolate cellulolytic bacteria from estuary water of Siak watershed in  Bengkalis  area and to determine its  cellulolytic activity and specific activity. A total of 13 isolates of cellulolytic bacteria have  been isolated using commercial cellulose substrate containing 1% w/v carboxymethyl cellulose (CMC). We found that 11 of the 13 isolates were Gram negative bacteria, while others were Gram positive. The isolate of C3 from Pelabuhan Roro showed the highest cellulolytic activity [(1.701 ±  0.166)  x 10-3 U/mL]  which was  not significantly different (p≤0.05) with cellulolytic activity of the  A3  isolate  [(1.605 ± 0.041) x 10-3 U/mL]. The C3 isolate also had the highest Cellulolytic specific activity [ (31.037  ± 4026) x 10-3 U/mg] and it was significant (p≥0.05) different with other isolates
TOTAL FENOLIK, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN PRODUK TEH HIJAU DAN TEH HITAM TANAMAN BANGUN-BANGUN (Coleus amboinicus) DENGAN PERLAKUAN ETT REMPAH-REMPAH Restauli Br Siburian; Christine Jose; Ganis Fia Kartika
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Coleus  amboinicus  is a plant with good antioxidant content . Antioxidants are very important  in preventing various diseases caused by free radicals.   The high antioxidant content of plant  C. amboinicus  potentially  used as raw material for making herbal tea products, such as  green tea and black tea. The objective of this research was to analyze the  total phenolics, flavonoids and antioxidant activity of green tea and black tea prepared from  C. amboinicus  plants with two different treatment, fermented plant extract (FPE) spice and control (water).  The results showed that the total phenolic and flavonoid content  of each  treatment  was different. The total phenolic content of control treatment and FPE spice green tea and black tea products  were  16,660 ± 0.251 mg AGE/g DW; 10.510 ± 0.737 mg AGE/g DW and 14,219 ± 0,198 mg AGE/g DW; 8.062 ± 0.334 mg AGE / g DW. Were  the total flavonoids treatment control and FPE spice green tea and black tea is 3.522 ± 0.077 mg CTE/g DW; 2.141 ± 0.104  mg CTE/g DW and 2.278 ± 0.057 mg CTE/g DW; 2.077 ± 0.044 mg CTE/g DW.  The content  of antioxidant activity by FTC method is highest shown  in the control treatme nt compared with ETT RR good green tea products and  black tea.  It can be concluded that green tea product of  C. amboinicus  is better  source of flavonoids, phenolics and antioxidant activity compared with the black tea product.
Co-Authors -, Sulismayati Andi Dahliaty Bintal Amin Fitri Rahayu Ganis Fia Kartika Hilwan Yuda Teruna Jasmadi ' Jasril Jasril Restauli Br Siburian Riski Novera Yenita Sarlly Deviani Usna Juwita Waidil Anuar Yondra Arif D Yuli Haryani Yuli Haryani Yum Eryanti