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All Journal Nexus Kedokteran Komunitas Nexus Biomedika Nexus Kedokteran Translasional
Sutartinah Sri Handayani
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Hubungan Antara Jentik Jentik Nyamuk dengan Angka Kejadian DBD di Kecamatan Ngemplak Salman Alfarisy; Sephendra Lutfi; Yosia Yonggara; Adelia Laksita Dewi; Afifah Syifa; Sutartinah Sri Handayani; Eko Widatik; Noor Alifah
Nexus Kedokteran Komunitas Vol 7, No 2 (2018): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Pendahuluan: Pencegahan dan pemberantasan penyakit menular dapat dilakukan dengan melaksanakan kegiatan surveilans epidemiologi. Penyakit menular, seperti demam berdarah dapat diatasi dengan melakukan penyelidikan epidemiologi untuk mengetahui faktor-faktor yang dapat menyebabkan penyakit terjadi. Data Puskesmas Ngemplak di Boyolali menunjukkan adanya peningkatan kejadian demam berdarah di awal tahun 2019. Incidence rate pada tahun 2018 adalah 14,8 per 100.000 penduduk dan pada tahun 2019 menjadi 17,37. Penelitian ini bertujuan untuk mengamati kepadatan jentik di lingkungan dengan radius 200 meter dan angka kejadian demam berdarah. Metode: Metode penelitian menggunakan metode kuantitatif deskriptif berupa survei dengan cara cross-sectional. Hasil: Hasil penelitian menunjukkan bahwa keberadaan jentik dalam daerah radius 200 meter meningkatkan risiko demam berdarah menjadi 5,3 kali lebih besar. Kesimpulan: Untuk mengatasi kejadian demam berdarah, ke depannya diperlukan adanya kerja sama antara warga dan pihak puskesmas dalam mencegah penularan dan mengobati demam berdarah.Kata Kunci: Larvae, House Index, Dengue Fever, Ngemplak Sub-district, Boyolali
The Effect of Drumstick/Horseradish (Moringa oleiferaLam) Leaves Ethanol Extract to The Mortality of Anopheles aconitus L. Larvae Yuzana Maung; Sutartinah Sri Handayani; Lukman Aryoseto ..........
Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: The use of synthetic insecticides to control Anopheles aconitus L. vector has negative impacts to nature dan resistancy, so it is necessary to use botanical larvicides as alternative substance to replace synthetic insecticides. Drumstick/Horseradish (Moringa oleifera Lam) leavesare loaded with saponin, tanin, flavonoid, dan alkaloid which have larvicide effects. The aim of this research was to find out the effect of Moringa oleifera Lam leaves ethanol extract on mortality of Anopheles aconitus L. larvae. Methods: This research is theexperimental laboratoricwith posttest only control group design which was conductedon 08-17 November 2016 at B2P2VRP, Salatiga, Central Java. In this research, ethanol extract of Moringa oleifera Lam leaves was used as larvicide to Anopheles aconitus L. third instar larvae. Larva was divided into 6 groups, each group contained 25 larvaes. For one control group was added 100ml distilled water,while the other 5 groups were added different concentration level of Moringa oleifera Lam leaves ethanol extract (1, 10, 20, 30, 40 mg per 100 ml of distilled water). Each test groups were repeated 4 times in this research. Observation was conducted after 48 hours exposure of extract and the numbers of larvae mortility were counted ofeach experimental group. The data were analyzed by using Kruskal-Wallis dan Probit tests. Results:The result of data analyzed by Kruskal-Wallis test indicates that there was significant difference on theaverage mortility of Anopheles aconitus L. larvae among 6 experimental groups. The result of Probit analysis indicate 50% of larvae mortality in 83.675 mg/100 ml (LC50) and 90% in 15318.159 mg/100 ml (LC90). Conclusions:Drumstick/Horseradish (Moringa oleifera Lam) leaves ethanol extract has mortalityeffect on Anopheles aconitus L. third instar larvae. Keywords: ethanol extract of Drumstick/Horseradish leaves, larvicide, Anopheles aconitus L. third instar larvae
The Difference of Efficacy Between Granule Form of Pandan Wangi Leaf (Pandanus amaryllifolius Roxb.) Extract and Abate Against Aedes aegypti L. Dewi Nur Khotimah; Sutartinah Sri Handayani; Sri Haryati
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Abatisasi is considered quite effective against Aedes aegypti L. as a dengue hemorrhaegic fever vector. Improperly use of abate dose can interfere human health and prolonged use can cause larvae resistance so that need an alternative larvicide. Granule of pandan wangi leaf extract (Pandanus amaryllifolius Roxb.) has a potention to be an alternative larvicide. This study aims to determine the effectiveness differences between pandan wangi leaf extract granule with abate against Aedes aegypti L. Methods: This study uses a quasi-experimental method with the approach posttest only control group design. This study was conducted in September and October 2014 in B2P2VRP Salatiga using the inclusion and exclusion criteria have been determined. The sampling technique used is incidental sampling. The independent variable is abate and pandan wangi leaf extract granule, while the dependent variable is the number of Aedes aegypti L. larvae instar III that be dead. Researcher compare into two different groups, namely pandan wangi leaf extract granule with various concentrations and abate. The data analysis used is One Way ANOVA continuing with LSD test and Probit Analysis. Results: The effectiveness of pandan wangi leaf extract granule is more lower than abate. The result of One Way ANOVA test shows a significance value p <0:01 (p <0.05), which means that there is a significant difference in mortality between the treatment group of larvae and the differences is statistically significant. The result of LSD test shows that abate 1% is significantly different with pandan wangi leaf extract granule 1.5%, 3.5% and 8%, while abate 1% is not significantly different with pandan wangi leaf extract granule 12.5% and 17%. The result of Probit Analysis shows LC50 is 7.007% and LC99 is 94,745%. Conclusions: There is an effectiveness difference between pandan wangi leaf extract granule with abate to against Aedes ageypti L. larvae. Keywords: Aedes aegypti L., pandan wangi leaf extract granule, abate.
The Effect Of Ethanol Extract Of Garden Balsams Leaves (Impatiens balsamina) To Mortality Of Anopheles aconitus Larvae Mutiani Rizki; Sutartinah Sri Handayani; Dian Nugroho
Nexus Kedokteran Translasional Vol 5, No 2 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Introduction: The prevalence of malaria in Indonesia is still high. One of the vector which transmitted malaria in Indonesia is Anopheles aconitus. During this time, abate (temephos) was being used to eliminate the mosquito larvae. The long-term use of abate (temephos) is not good for environment and perhaps may cause resistance on mosquito. Garden Balsams leaves (Impatiens balsamina) contain substances which can be larvacide, so the researcher want to prove whether the ethanol extract of Garden Balsams leaves (Impatiens balsamina) have effect to mortality of Anopheles aconitus. Methods: This research used laboratory experimental method with the post test only control group design. The object of this research was Anopheles aconitus larvae which divided into 7 groups, each group contain 25 larvae and this research was replicated 4 times. For the negative control, 100 ml aquadest was used. And for the other 6 groups, contain 300 mg, 500 mg, 700 mg, 900 mg, 1100 mg, 1300 mg extract in 100 ml aquadest. The data was analyzed by Linear Regression and Probit test. Results : Linear Regression test show that the percentage of extract effect to larvae mortality is 88,3% and the rest 11.7% was effected by the other variable. The linear equation is Y= 3.116 + 0,02X . Probit test show LC50 is 405.580 mg/100 ml and LC99 is 1352.177 mg/100 ml. Conclusion: There is effect of ethanol extract of Garden Balsams leaves (Impatiens balsamina) to the mortality of Anopheles aconitus larvae. The LC50 is 405.580 mg/100 ml and LC99 is 1352.177 mg/100 ml. Keywords: extract of Garden Balsams leaves, mortality, Anopheles aconitus larvae
The Effect of Indonesian Bayleafs [Syzygium polyanthum (Wight.) Walp] Ethanol 70% Extract Granule to the Mortality of Anopheles aconitus Larvae Muhamad Dwi Heriansyah; Sutartinah Sri Handayani; Novianto Adi Nugroho
Nexus Kedokteran Translasional Vol 5, No 1 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Introduction: Due to the high rate of malaria incidence in Indonesia and antimalaria drugs resistance causes increment of mortality and morbidity rate for the patients. Most people to cope with the situation, tend to use larvicide to manage the vector but in the process it face some difficulity that not only it can harm the environment but also tend to develop resistance. This research will focus to find an alternative larvicide made by plant to substitute the synthetic larvicide. This research tries to determine the effect of Indonesian bayleaf granule extract to the mortality of Anopheles aconitus larvae. Methods: This research used laboratory experimental design with the post test only control group design. This experiment was used Indonesian Bayleafs [Syzygium polyanthum (Wight.) Walp] granule ethanol extract as larvicide against Anopheles aconitus larvae. Larvae were divided into 9 groups, each group contains 25 larvaes. For the negative control group 100 ml aquadest was used. The other 8 groups which 1 group with 3500 mg dextrin was added, and 7 group with different granule concentration (1700 mg, 2100 mg, 2500 mg, 2900 mg, 3300 mg, 3700 mg, 4100 mg). This research conducted with 4 times replication based on WHO guideline. The observation was held after 24 hours the experiment are conducted, and then the number of dead larvae was counted. The data was analysed with ANOVA, LSD and Probit test. Results: ANOVA test, LSD, was used to analyse the data it showed that there are significant difference of larvae mortality between the experimental group. Probit test that was also used showed that the LC50 is is 2,005.3 mg/100 ml and the LC99 is 7,730.41 mg/100 ml. Conclusion: There was effect of Indonesian bayleaf [Syzygium polyanthum (Wight.) Walp] granule ethanol extract to the mortality of Anopheles aconitus. The LC50 is 2,005.3 mg/100 ml and the LC99 is 7,730.41 mg/100 ml. Keyword: granules, Indonesian bayleaf extract, Anopheles aconitus larvae, mortality
Co-Authors Adelia Laksita Dewi Afifah Syifa Dewi Nur Khotimah Dian Nugroho Eko Widatik Lukman Aryoseto .......... Muhamad Dwi Heriansyah Mutiani Rizki Noor Alifah Novianto Adi Nugroho Salman Alfarisy Sephendra Lutfi Sri Haryati Yosia Yonggara Yuzana Maung