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Pengaruh Ekstrak Biji Kedelai Putih (Glycine max) terhadap Waktu Kematian Cacing Gelang Babi (Ascaris suum) in Vitro Pritami .; Sri Haryati; Bambang Sukilarso Sakiman
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : White Soy beans (Glycine max) contain saponin and tripsin inhibitor that have been known having anthelmintic effect. This study aimed to determine the effect of White Soy beans extract toward death time of Ascaris suum, in vitro. Methods : The study was a laboratory experimental research using post test only controlled group design. The subjects were an adult male and female worms (Ascaris suum) that their body length were around 15 cm 35 cm. The sampling technique used was convenience sampling. The subject was arranged into 6 groups, each group consisted of 4 worms, replication was performed 4 times. NaCl 0.9 % and pirantel pamoat 5 mg/ml was added in the negative control group and in the positive control group, in a row.While white soy beans extract of concentration of 60%, 70%, 80%, and 90% w/v were added to the treatment group. Worms were immersed in 25 ml test solution and incubated at 37C. The subjects were observed every 10 minutes and the number of dead worms was counted. Data were analyzed with linear regression and Pearson correlation. Results : The data of observation showed that total death mean time of Ascaris suum in the negative control and positive control were 2817.5 minutes and 42.5 minutes, while in the treatment group of concentration of 60% , 70% , 80% , and 90% w/v of white soy beans extract were 422.5 minutes, 370 minutes, 300 minutes, and 255 minutes, successively. The Pearson correlation test showed very close relationship between variation of the concentration and time of death worms (r = - 0.962). The linear regession test showed significantly (p<0.001) and the regression equation was Y = 2653.400 30.340 X and R square was 0.926. Conclusion : White soy beans (Glycine max) extract had effects to the mortality of Ascaris suum in vitro. The most effective white soy beans extract was 90% (w/v) although it was less effective than pirantel pamoat. Keywords : White Soy beans (Glycine max) Extract, Ascaris suum, death time.

Pengaruh Ekstrak Daun Salam (Syzygium polyanthum Wight) Terhadap Waktu Kematian Ascaris suum,Goeze In Vitro Gagat Ragil Andaru P; Bambang Sukilarso Sakiman; Sri Haryati
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Syzygium polyanthum Wight leaves contain tannin that has been known to have anthelmintic effect. This study aimed to determine the effect of Syzygium polyanthum Wight leaves extract toward death time of Ascaris suum, Goeze In Vitro Methods: The study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were divided into 7 groups, each group consisted of 4 worms, replication was performed 4 times. Saline solution was used in negative control group, pirantel pamoate 5 mg/ml was used in positive control group, while the treatment group used Syzygium polyanthum Wight leaves extract concentration of 70%, 75%, 80%, 85%, and 90%. Worms were immersed in 25 mL of the test solution and incubated at 37C. Observations were done every 1 hour and the number of dead worms was counted. Data were analyzed with regression linier and probit analysis. Results: Observations of total deaths mean time Ascaris suum, Goeze sp on negative control was for 96 hours, on the positive control was for 2 hours 30 minutes, and on the concentration of 70%, 75%, 80%, 85%, and 90% of Syzygium polyanthum Wight leaves extract, each was for 10 hours 15 minutes, 8 hours, 6 hours, 4 hours 45 minutes and 3 hours, consecutively. Linear regression test results showed that the variation of the concentration affected long worm death. Probit analysis results showed that LC50 and LT50 of Syzygium polyanthum Wight leaves extract, each was 76.228% and 3 hours 36 minutes. Conclusions:Based on this study, it could be concluded that of Syzygium polyanthum Wight leaves extract affected mortality of Ascaris suum, Goeze sp in vitro. The increasing of extract concentration was inversely proportional with the death time of worms Keywords: Syzygium polyanthum Wight leaves extract, Ascaris suum,Goeze.

Pengaruh Ekstrak Buah Jambu Biji Merah terhadap Kerusakan Struktur Histologis Hepar Mencit yang Diinduksi Asetaminofen Vasa Adi Wisnu Wardana; Suyatmi .; Bambang Sukilarso Sakiman
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Antioxidants in guava fruit are vitamin C, flavanoid, and lycopene which are estimated having hepato-protective function. The objectives of this research are to know the effect of guava fruit extract and in various doses on mice liver damage induced by acetaminophen. Methods: Samples of 28 male mice, Swiss webster type, 2-3 months old age and + 20gr of each weight, were divided into 4 groups of 7 mice. Guava fruit extract was given for 14 days in a row, while acetaminophen in toxic dose was given on the 12th, 13th, and 14th day. The score of hepatocyte damage was determined by pyknosis, karyorhexis, and karyolysis. The data was analized by One-Way ANOVA test (?= 0,05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences(LSD) test(?= 0,05). Results:The group with the highest average damage of hepatocyte is KP1(84,233,207), and the groups with decrease average damage of hepatocyte are KP2 (48,762,964) and KP3(35,232,755). The results of One-Way ANOVA test showed a significant difference in all group (p<0.001). The statistical data result showed a significant difference of hepatic damage between K-P1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Guava fruit extract can decrease the histological damage of the hepatocyte of mice induced by acetaminophen. The higher dose of guava fruit extract, the higher protection effect againts liver histological damage from acetaminophen toxic on mice. Key words: guava fruit extract, acetaminophen, histological damage, hepatocyte.

Pengaruh Ekstrak Wortel (Daucus carota L.) terhadap Waktu Kematian Cacing Ascaris suum, Gooze in Vitro Aryo Seno; Sri Haryati; Bambang Sukilarso Sakiman
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : Carrots contain quercetin that can be used as antihelmintik. Quercetin is a class of flavonoid glycosides. This study aimed to determine the effect of carrots extract (Daucus carota, L.) against the death time of Ascaris suum, Goeze in vitro. Methods :This study was a laboratory experimental research using post-test only controlled group design. Subjects were Ascaris suum, Goeze which were still in active movement. Sampling technique used in this study was convenience sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replication was done 4 times. Pyrantel pamoate 5mg/ml and physiological saline were successively added to the test solution of positive control and negative control group, while carrots Extract (Daucus carota, Linn) of 70%, 75%, 80%, 85% and 90% were added to the test solution of treatment groups. Worms were soaked in 25 ml of test solution and incubated at 37C. Observations were done every hour until all the worms died. Data were analyzed using Pearson correlation and linear regression. Results : There was a significant correlation between the concentration variables and time of death of the worms variable (p value of Pearson correlation was 0.000 (<0.050). The higher concentration of the carrot extract was the shorter time of death of worms (Correlation coefficient of concentration variable and time of death of the worms variable was - 0.960). The linear regression equation was Y = 53.775 - 0.609X. Conclusion : The Carrots extract (Daucus carota, Linn) had effects on accelerating the time of death of Ascaris suum, Gooze in vitro. Keywords : Daucus carota, Linn extract, Ascaris suum, Goeze

Correlation between Infection of Soil Transmitted Helminths (STH) and Peripheral Blood Eosinophil Levels amongElementary Student at Barengan Village, Teras Subdistrict, Boyolali. Aulia Nadhiasari; Bambang Sukilarso Sakiman; Paramasari Dirgahayu
Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: The Parasitic infection, caused by intestinal helminths, accounts for significans burden of human disease in developing countries including Indonesia. In chronic infection, eosinophilia occurs as protection against helminth parasites. Increasing eosinophils is often associated with diseases, such as allergy, nephrotic syndromes, leoffler diseases, and also helminthiasis. However, there are little data to prove this unequivocally. This study aimed to determine the correlation between soil transmitted helminths infections and the levels of peripheral blood eosinophil among elementary students at Barengan Village, Teras Subdistrict, Boyolali Methods: This study was conducted by an observational analytic research in a cross sectional approach at Elementary School students in Barengan Village, Teras Subdistrict, Boyolali on periode of October-November 2014. We conducted on 74 samples according to the inclusion and exclusion criteria. The data were collected from stool, peripheral blood and questionnaires and then were statistically analyzed with Chi Square test to determine their correlation. Results: Our study showed that the prevalence of helminthiasis among student was 47.3% and Chi Square Analyzes between STH infection and eosinophilia had the value of p=0.041 with Odds Ratio= 2.695. Conclusions:Barengan Village, Teras Subdistrict, Boyolali is still an endemic area for helminthiasis (47.3%). This study shows correlation between soil transmitted helminthes infections and increasing the levels of peripheral blood eosinophil among elementary students at Barengan Village, Teras Subdistrict, Boyolali. Keywords: Soil transmitted helminths, eosinophil, elementarystudents of SD Barengan

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