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Bambang Widjokongko
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Medicine & Pharmacology

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Protective Effect of Orthosiphon stamineus Benth Leaves Extract against Reheated palm oil-induced Liver Damage in Mice Fitroh Annisah; Bambang Widjokongko; Siti Utari
Nexus Kedokteran Translasional Vol 2, No 2 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Orthosiphon stamineus Benth contains polyphenols, terpenoids, oleanolat acids, and sterols which were thought to protect the liver from free radicals. In present study, Orthosiphon stamineus Benth leaves extract was evaluated for its protective effect on reheated palm oil -induced liver damage in mice. Methods: This was laboratory experimental research with the post test only controlled group design. Twenty five male Swiss webster mice were equally divided into five groups: group I (Kn) received aquadest, group II (Kp) received reheated palm oil, and grup IIIV (P1-P3) received reheated palm oil and multilevel doses of Orthosiphon stamineus Benth leaves extract (3.5 mg, 7 mg, and 14 mg) for 14 days. Mice were sacrificed on the 15th day and histological preparation was made to evaluate histological damage on liver. Liver histological features were assessed by counting the number of hepatocyte on centrolobuler zone undergoing pyknosis, karyorhexis, and karyolysis. Data were analyzed using the One Way ANOVA test (? = 0.05) and the Post Hoc Multiple Comparisons (LSD) test (? = 0.05). Results: The mean liver histological damage score was significantly higher on group of Kp= 74.40 2.074, while group of Kn= 38.20 3,564 as the less. P2 group had fewer numbers of liver histological damage (57.00 4.472) compared to the P1 group (61.00 2.550) and the P3 group (58.60 4.775). The results of One Way ANOVA test showed significant difference between the five groups (p = 0.000) and the results of LSD test showed significant differences between each other group (p < 0.005), except the group of P1-P2, P1-P3, and P2-P3 (p > 0.005). Conclusions: Orthosiphon stamineus Benth leaves extract showed protective effect against the hepatotoxicity induced by reheated palm oil in mice. Orthosiphon stamineus Benth leaves extract with the dose of 7 mg/25 g body weight of mice showed a higher hepatoprotective effect than the dose of 3.5 mg/25 g body weight of mice and 14 mg/25 g body weight of mice. Keywords: Orthosiphon stamineus Benth leaves extract, reheated palm oil, histologic liver damage.
The Effect of Mangosteen Peel (Garcinia mangostana L) Extract Against Mice (Mus musculus) Kidney Cell Damage Induced by Paracetamol Namira Qisthina; Bambang Widjokongko; Sigit Setyawan
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Mangosteen peel (Garcinia mangostana L) contains antioxidant such as xanthone core, ?-mangostin, ?-mangostin, ?- mangostin, and garcinone which is thought to be nefroprotector. This study is aimed to investigate the mangosteen peel (Garcinia mangostana L) extracts nefroprotector effect against mice (Mus musculus) kidney cell damage induced by Paracetamol. Methods: This is an experimental laboratory study with post test only controlled group design. Swiss Webster mice weighing around 20 g were selected as sample. Those mice were divided into 4 groups randomly, each group consist of 7 mice. Incidential sampling was used as a sampling technique. Mice in control group (KK) and first treatment group (KP1) were given aquades, while second treatment group (KP2) and third treatment group (KP3) were given 10 mg and 15 mg of mangosteen peel extract respectively. KP1, KP2, and KP3 were given 0.1 ml paracetamol on the 8th, 9th, and 10th day of the treatment. Mice were sacrificed on 4th day and the kidneys were made into preparate using paraffin block and HE staining method. Histological kidney cells were assesed by the sum of pyknosis, karyorrhexis, and karyolysis cells. Data were analyzed using One Way ANOVA test continued by Post Hoc Multiple Comparisons (LSD). Result: One-Way ANOVAs test result showed a significant differences between four groups. LSDs test result showed a significant differences between KK-KP1, KK-KP2, KK-KP3,KP1- KP2, KP1- KP3, KP2-KP3. Conclusion: Mangosteen peel extract can prevent mices kidney cell damage induced by parasetamol. Keywords: Mangosteen peel extract, parasetamol, mices kidney cell damage
Co-Authors Fitroh Annisah Namira Qisthina Sigit Setyawan Siti Utari