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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak dan Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Ilmu dan Teknologi Peternakan Tropis Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Panrita Abdi - Jurnal Pengabdian pada Masyarakat Hasanuddin Journal of Animal Science (HAJAS)
Gustina, Sri
Universitas Sulawesi Barat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Veterinary Other

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Efektivitas Insulin-Like Growth Factor-I (IGF-I) dalam Media Maturasi In Vitro Pada Pematangan Inti dan Fertilisasi Oosit Sapi Bali . Hasbi; Sri Gustina; Ni Wayan Kurniani Karja; Iman Supriatna; Mohamad Agus Setiadi
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.643 KB) | DOI: 10.29244/avi.6.1.24-29

Abstract

Penelitian ini bertujuan mengetahui efektivitas insulin-like growth factor–I (IGF-I) pada tingkat pematangan inti dan fertilisasi oosit sapi bali. Penelitian ini dibagi dalam dua tahap. Tahap I, oosit dimatangkan secara in vitro dalam media 199 dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I. Tahap II, oosit dimatangkan dalam media seperti pada penelitian tahap I, kemudian difertilisasi secara in vitro untuk mengamati pembentukan pronukleus. Hasil penelitian tahap I menunjukkan bahwa tidak terdapat perbedaan jumLah oosit yang mencapai tahap metaphase II (MII) dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I dalam media maturasi. Berturut-turut adalah 80,6±7,6%; 81,5±8,6%; 87,5±6,9%; dan 84,1±12,4%. Penelitian tahap II menunjukkan bahwa tingkat fertilisasi pada penambahan 100 ng/mL IGF-I dalam media maturasi nyata lebih tinggi (P<0,05) dibandingkan dengan kontrol dan penambahan 50 ng/mL IGF-I, yaitu berturut-turut 78,3±6,6%, 67,1±8,9%, dan 64,6±6,0% untuk dosis 100, 0, dan 50 ng/mL. Akan tetapi, peningkatan dosis pemberian IGF-I menjadi 150 ng/mL tidak meningkatkan tingkat fertilisasi yaitu 73,5±9,3%. Dari hasil penelitian disimpulkan bahwapenambahan IGF-I dalam media maturasi tidak mampu meningkatkan jumLah oosit yang mencapai tahap MII, namun penambahan 100 ng/mL IGF-I dapat meningkatkan jumLah oosit yang terfertilisasi.
Characteristics of Libido and Testosterone Concentration of Polled and Horned Bali Bulls after GnRH Injection Hasbi Hasbi; Herry Sonjaya; Sudirman Baco; Reski Amalia; Sri Gustina
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2851

Abstract

Bali cattle are one of the original Indonesian germplasm, which in its development were found to be hornless or polled. Polled are Balinese cattle whose horns do not grow naturally, but have the same characteristics as those with horns. However, there are indications that polled have a lower libido. The purpose of this study was to determine the characteristics of libido and testosterone concentration after gonadotrophin releasing hormone (GnRH) injection. In this study each of 7 male polled and horned Bali cattle aged 3.5-6 years were used. Libido characteristics were observed by recording the first time the male fondled the teaser until ejaculation, while the testosterone concentration was measured using blood plasma taken on day 0 or before injection, day 7th, and day 14th after GnRH injection. Testosterone analysis used enzyme linked immunosorbent assay (ELISA) method. The results showed that the libido of polled was not different (P>0.05) compared to that of the horned on the 0, 7th and 14th days after GnRH injection. However, in polled on the 7th day after GnRH injection, it was significantly lower than on day 0 and 14, but on day 0 it was not different with day 14. Testosterone concentration on day 7 after injection of GnRH was not different (P>0.05) compared to day 0 both in polled and horned bulls, but on day 14 after injection was significantly higher (P<0.05) in horned than polled. In conclusion, polled and horned Bali bulls had high libido with a score of +1, but on the 7th day after GnRH injection, polled had lower libido than horns. The testosterone concentrations of polled and horned on day 7 after GnRH injection were not different compared to day 0, while the 14th day was higher in horned bull.
Androgen Regulation in Spermatogenesis to Increase Male Fertility Hasbi Hasbi; Sri Gustina
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 28, No 1 (2018): March 2018
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.58 KB) | DOI: 10.14334/wartazoa.v28i1.1643

Abstract

Male fertility is affected by quantity and quality of sperm which controlled by androgens (testosterone and 5α-dihydrotestosterone) mediated by androgen receptors (AR). Androgen receptors belong to receptor group of steroid hormone and a group of ligand-activated nuclear receptor superfamily. This paper explains androgen hormone and its regulation in spermatogenesis to increase male fertility. Regulation of androgen hormone in spermatogenesis include initiation of spermatogenesis, proliferation and maturation of Sertoli cells, germ cell development, spermatogonia, meiosis, and spermiogenesis. The role of androgen hormone in regulation of spermatogenesis is influenced by AR, luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels. Disruption of spermatogenesis will cause low male fertility. However, low concentrations of AR, LH and FSH could be enhanced by exogenous gonadotrophine releasing hormone (GnRH), LH, FSH, and testosterone to increase male fertility.
Motilitas dan Viabilitas Spermatozoa Sapi Bali Polled dan Bertanduk pada Setiap Tahapan Proses Pembekuan Sri Gustina; Hasbi Hasbi; Herry Sonjaya; Sudirman Baco; Abdul Latief Toleng; Mutmainna Mutmainna; Sitti Farida
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.01 KB) | DOI: 10.33772/jitro.v9i1.18417

Abstract

ABSTRAKSapi bali merupakan sapi yang dikembangkan, dimanfaatkan dan dilestarikan sebagai sumberdaya ternak asli. Saat ini telah dikembangkan populasi sapi bali polled. Sapi bali polled merupakan sapi bali yang tanduknya tidak bertumbuh secara alami. Tujuan dari penelitian ini adalah untuk mengetahui persentase motilitas dan viabilitas spermatozoa sapi bali polled dan bertanduk pada setiap tahapan proses pembekuan. Koleksi semen dilakukan dengan metode vagina buatan. Semen hasil koleksi selanjutnya dievaluasi secara makroskopik dan mikroskopik. Semen yang memenuhi persyaratan diproses lebih lanjut untuk dibekukan. Pejantan yang digunakan sebanyak 3 ekor polled dan 3 ekor bertanduk dengan umur 3,5 sampai 6 tahun. Parameter yang diamati meliputi motilitas dan viabilitas selama proses pembekuan sampai post thawing. Data yang diperoleh diuji dengan uji-t (sampel T-Test independen). Hasil penelitian menunjukkan bahwa tidak ada perbedaan nyata (p>0,05) antara sapi bali polled dan bertanduk terhadap persentase motilitas setelah pengenceran (63,33±1,92 vs 65,00±0,00). Namun, terdapat perbedaan yang nyata (p<0,05) setelah equilibrasi yaitu 54,99±1,35 vs 60,00±0,00 dan post thawing 45,41±0,83 vs 49,58±0,83. Persentase viabilitas setelah pengenceran dan setelah equilibrasi tidak berbeda nyata (p>0.05) yaitu 81,94±4,05 vs 86,51±1,26 dan 72,80±6,80 vs 80,17±2,74. Namun, pada post thawing terdapat perbedaan yang nyata (p<0,05) yaitu 56,95±3,74 vs 72,55±2,00. Kesimpulannya bahwa persentase motilitas dan viabilitas spermatozoa sapi bali polled lebih rendah dibandingkan yang bertanduk.Kata Kunci: motilitas, sapi bali polled, sapi bali bertanduk, spermatozoa, viabilitasMotility and Viability of Spermatozoa of Polled and Horned Bali Bulls at Each Stage of the Freezing Process ABSTRACTBali cattle are cows and bulls that are developed, utilized and preserved as a native livestock resource. Currently, a population of polled bali cattle has been developed. Polled bali cattle are bali cattle whose have horns do not grow naturally. The purpose of this study was to determine the percentage of motility and viability of spermatozoa of polled and horned bali cattle at each stage of the freezing process. Semen collection was carried out by the artificial vaginal method. The collected semen was then evaluated macroscopically and microscopically. The semen that meets the requirements is further processed to be frozen. The bulls used were 3 polleds and 3 horneds with an age of 3.5 to 6 years. Parameters observed included motility and viability during the freezing process until post thawing. The data obtained were tested by t-test (independent sample T-Test). The results of this study indicated that there was no significant difference (p>0.05) between polled and horned bali cattle on the percentage of motility after dilution (63.33 ± 1.92 vs 65.00 ± 0.00). However, there was a significant difference (p<0.05) after equilibration (54.99±1.35 vs 60.00±0.00), and post thawing (45.41±0.83 vs 49.58±0.83). The percentage of viability after dilution and after equilibration was not significantly different (p>0.05), 81.94 ± 4.05 vs 86.51 ± 1.26 and 72.80 ± 6.80 vs 80.17 ± 2.74. However, there was a significant difference in post thawing (p<0.05), 56.95 ± 3.74 vs 72.55 ± 2.00. It can be concluded that the percentage of motility and viability of spermatozoa of polled bali cattle is lower than horned.Keywords: motility, polled bali bull, horned bali bull, spermatozoa, viability
Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan Sri Gustina; Hasbi Hasbi; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7327.448 KB) | DOI: 10.22146/jsv.34695

Abstract

In vitro embryo production relies on the quality of oocytes, however the quality is subjected to ovaries reproduction cycle. This study was conducted to observe the potency of buffalo ovaries from various reproductive cycle in producing quality oocytes. Collected pairs of ovaries from slaughter house were weighed and grouped of 5 according to the cycle. Oocytes were collected by slicing techniques, then selected based on quality. The results showed the average weight of buffalo ovaries of (+CL, +FD); (+CL, -FD); (-CL, +FD); (-CL, -FD) are 7.2 g; 5.5 g; 4.1 g; and 4.5 g respectively. No significant quality difference of produced oocytes between ovaries cycles (P>0.5). Good quality of collected oocytes were only 40-55%. Approximately 2-5 oocytes of grade A and 1-5 oocytes of grade B can be collected per pair of ovaries. 
Upaya Pembinaan Masyarakat Dalam Rangka Pengembangan Susu Kambing Pasteurisasi (Suke) pada Kelompok Tani di Desa Lambanan, Kecamatan Balanipa, Kabupaten Polewali Mandar, Sulawesi Barat Sri Gustina; Sulmiyati Sulmiyati; Magfrah Magfrah; Marsudi Marsudi
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 1 (2016): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.32 KB) | DOI: 10.22146/jpkm.22227

Abstract

Problems faced by the society in Lambanan village are the lack of knowledge and skills of farmers in managing the maintenance goat, the unability of utilizing and processing goat milk and most societies work as goat breeder and farmer. Purpose of this community dedication is to provide skills and the knowledge for the farmers in the Lambanan village about techniques and milking ways as well as handling and processing milk of goat. Method used is partisipative elucidation followed by demonstration and practice. Te training activities which are conducted on 25—26 June 2016 in main hall of Lambanan village ofce, began with in-class learning about maintenance management, handling, processing and marketing management the goat of PE, then followed by demonstrating outside about technique of milking and processing milk of goat pasteurization. Te products of pasteurized goat milk were packaged in glass that was commercialized with label Suke. Te conclusion of this activity shows that the training activities looked very enthusiastic to join this training and actively ask about goat milk processing.
Effect of FSH and PMSG Additions on Maturation and Fertilization Rates of Bali Cattle Oocytes Hasbi Hasbi; Herry Sonjaya; Andi Arya Pawarekki; Sri Gustina
Hasanuddin Journal of Animal Science (HAJAS) Vol. 2 No. 2 (2020)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v2i2.13735

Abstract

The process of in vitro oocyte maturation requires stimulation of gonadotropin hormones, as well as in vivo. There are two gonadotropin hormones that are available in the market and can be used in the process, Follicle Stimulating Hormone (FSH) and Pregnant Mare Serum Gonadotropin (PMSG). This study aimed to determine the effect of the addition of FSH and PMSG hormones in the maturation medium on the in vitro maturation and fertilization rates of Bali cattle oocytes. This study had two treatments, the addition of FSH and PMSG, each of which was carried out 4 times. Oocytes matured in basic medium, M-199 plus Bovine Serum Albumin (BSA) 10 IU/ml, and 50 µg/ml Gentamycin. Furthermore, the matured oocytes fertilized in 80 μl drop of sperms. The data were analyzed by Chi Square test to determine the differences in the level of maturation and fertilization of Bali cattle oocytes in vitro. The results of this study indicated that there was no significant difference (P>0.05) between the addition of FSH and PMSG hormones in the maturation medium to the nuclear maturation rate (81.4 ± 2.2 vs 81.2 ± 2.3), and the fertilization rate (42.2 ± 4.3 vs 24.6 ± 2.7). It can be concluded that in vitro maturation and fertilization rate of Bali cattle oocytes with the addition of FSH and PMSG hormones to the maturation medium are similar, but showed a tendency for higher fertilization rate in the medium supplemented with FSH.
Review: Comparative of monitoring estrus cycle in livestock: Hormonal features and ultrasound Hasbi Hasbi; Sri Gustina
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 1 (2020): April 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.01.02

Abstract

The estrus cycle is a period in which a female animal shows the ability to become pregnant, and this event will continue over some time. The estrus cycle has two different phases, the follicular phase characterized by the presence of graffian follicles which are classified as proestrus and estrus stages or periods from regression of the corpus luteum to ovulation, and luteal phase which is characterized by the presence of the corpus luteum which is classified as the metestrus and diestrus stage or period of ovulation to the corpus luteum regression. Proestrus is chained by decreasing the concentration of progesterone, followed by increasing the concentration of estradiol. Estrus is generally characterized by a very low concentration of progesterone, an increase in the concentration of LH, and a decrease in the concentration of estrogen. Metestrus is characterized by a low concentration of progesterone. Diestrus is characterized by an increase in the concentration of progesterone and an increase in steroid hormone production along with the maturation of the corpus luteum. The purpose of this review is to discuss the comparative monitoring of the estrus cycle in buffalo, dairy cows, sheep and camels related to hormonal features and ultrasonography.
PENINGKATAN LEVEL KEBERDAYAAN MITRA TERKAIT TEKNOLOGI PAKAN BERBASIS LIMBAH PERIKANAN DI KECAMATAN TINAMBUNG KABUPATEN POLEWALI MANDAR Sri Gustina; Chairul Rusyd Mahfud; Hendro Sukoco; Marsudi Marsudi; Hasbi Hasbi
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 8 No. 1 (2024): Jurnal Panrita Abdi - Januari 2024
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v8i1.23520

Abstract

Polewali Mandar Regency is one of the areas that produce quite a lot of fisheries. However, at the peak of the season, a lot of fish overmeasure and processing cesspits are wasted and not processed properly. Based on the problems, the PKM implementation team held training on processing fishery waste and making concentrate feed based on fish flour from the processing. The concentrate feed product is then used to improve the body condition of the Bali cows that have reproductive problems. The results of this activity, in addition, to concentrate feed products, there was also an increase in the level of empowerment of partner group members related to the use of fishery waste to be used as additional concentrate feed that could improve the reproductive performance of cattle.  ---  Kabupaten Polewali Mandar termasuk salah satu daerah penghasil perikanan yang cukup banyak. Akan tetapi, di puncak musim banyak sisa ikan beserta limbah pengolahan yang terbuang dan tidak diolah dengan baik. Dengan permasalahan tersebut, tim pelaksana PKM mengadakan pelatihan pengolahan limbah perikanan serta pembuatan pakan konsentrat berbasis tepung ikan hasil pengolahan tersebut. Produk pakan konsentrat yang dihasilkan kemudian digunakan untuk memperbaiki kondisi tubuh induk-induk sapi Bali yang memiliki masalah reproduksi. Hasil dari kegiatan ini, selain produk pakan konsentrat juga terjadi peningkatan level keberdayaan anggota kelompok mitra terkait pemanfaatan limbah perikanan untuk dijadikan sebagai tambahan pakan konsentrat yang bisa meningkatkan performa reproduksi ternak sapi. 
Co-Authors . Hasbi Abdul Latief Toleng Andi Arya Pawarekki Baco Sudirman Chairul Rusyd Mahfud H. Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hasbi Hendro Sukoco Herry Sonjaya Herry Sonjaya Herry Sonjaya Iman Supriatna M Agus Setiadi Magfrah Magfrah Marsudi Marsudi Mutmainna Mutmainna Ni Wayan Kurniani Karja Reski Amalia Sitti Farida Sudirman Baco Sulmiyati Sulmiyati