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All Journal Jurnal Ilmu Ternak dan Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

Maman Surachman

Unknown Affiliation

Author-ID : 2823819

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Articles

Role of seminal plasma in maintaining quality of ram epididymal spermatozoa preserved at low temperature (3–5oC) Muhammad Rizal; Maman Surachman; Herdis .; Achmad Selamet Aku
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, because the spermatozoa is motile and has ability of fertilizing the oocyte. The purpose of this research was to examine the effect of addition of seminal plasma on the quality of ram cauda epididymal spermatozoa preserved at 3–5oC. Collected-spermatozoa was divided into five parts then centrifuged at 3,000 rpm for 30 min and the supernatant was removed. One-fifth was diluted with Tris extender (Tris) and the remaining was added 0.5 ml ram seminal plasma then diluted with Tris extender (PS-Tris), 15% AndroMed + 85% distilled water (PS-AM15), 20% AndroMed + 80% distilled water (PS-AM20), and 25% AndroMed + 75% distilled water (PS-AM25), respectively. Quality of collected-spermatozoa including spermatozoa concentration (SC), percentages of motile spermatozoa (MS), live spermatozoa (LS), abnormal spermatozoa (AS), cytoplasmic droplet (CD), and intact plasma membrane (IPM) were evaluated. Percentages of MS, LS, and IPM of diluted-spermatozoa was evaluated every day during preserved at 3–5oC for three days. Results of this study showed that mean SC, MS, LS, AS, CD, and IPM of fresh spermatozoa were 11,660 million/ml, 65, 81, 7.6, 10.2, and 82.2%, respectively. Addition of seminal plasma in cauda epididymal spermatozoa prior to dilution could maintain the quality of spermatozoa during preserved at 3–5oC for three days. At day-4 of storage, percentages of MS, LS, and IPM for PS-Tris (43, 58, and 59.2%), PS-AM20 (40.5, 53.75, and 53.75%), and PS-AM25 (40, 54.8, and 55.2%) were significantly (P<0.05) higher than Tris (21, 34.8, and 33.6%) and PS-AM15 (20, 40, and 42.2%). In conclusion, addition of seminal plasma in ram cauda epididymal spermatozoa prior to dilution with Tris or 20 and 25% AndroMed extenders could maintain the quality of spermatozoa during preserved at 3–5oC for three days, and could be used for artificial insemination or in vitro embryo production programs. Key Words: Cauda Epididymal Spermatozoa, Seminal Plasma, Tris, AndroMed, Ram

Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC Muhammad Rizal; Herdis .; Maman Surachman; W. Marlene Mesang-Nalley
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck

Increasing of Reproduction Efficiency of Cattle Through the Application of Synchronization Technology Herdis .; Maman Surachman; Ida Kusuma; Epih R Suhana
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 1 (1999)
Publisher : Indonesian Center for Animal Research and Development

The constraint of cattle development in Indonesia is low productivity, which was caused by low reproduction efficiency. Unestroes and difficulty of heat detection influenced to the calving interval. This calving interval is one of the causal factors of low reproduction efficiency. The efforts to solve this problem are to control and to predict heat period using synchronization technology. Synchronization technology on catlle has been developed from corpus luteum enucleated, utilizing of gonadotropin, progesteron, estrogen, prostaglandine and modification of those hormones. Application method for synchronization could be done by intra musculair, subcutan implantation, intra vaginal and mixed with food. Each method has its own strength and weaknesses. However, to handle anestroes cases is more effective to use combination of progesteron, PGF2α and estrogen. In order to obtain reproduction efficiency it is suggested to take the anestroes in hand as soon as possible. Key words : Cattle, synchronization estrous

Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries) Herdis Herdis; Maman Surachman; Muhammad Rizal; Arief Boediono; Yulnawati Yulnawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.142

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations. Cement was collected from three mature rams using an artificial vagina once a week. After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively. The extended-cement was stored at 5oC. Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days. The results showed that addition of trehalose did not significantly improved the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days. In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.

Co-Authors Achmad Selamet Aku Anak Agung Gede Sugianthara Arief Boediono Epih R Suhana Herdis . herdis herdis Ida Kusuma Wilmientje Marlene Mesang Nalley YULNAWATI YULNAWATI

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