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Yusmaniar Yusmaniar
Poltekkes Kemenkes Jakarta 2
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Journal : Medical Journal of Indonesia

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Simultaneous detection of Legionella species and Legionella pneumophila by duplex PCR (dPCR) assay in cooling tower water samples from Jakarta, Indonesia Yasmon, Andi; Yusmaniar, Yusmaniar; Anis, Anis; Bela, Budiman
Medical Journal of Indonesia Vol 19, No 4 (2010): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.177 KB) | DOI: 10.13181/mji.v19i4.408

Abstract

Aim: Since culture method is time-consuming and has low  sensitivity, we developed a duplex PCR (dPCR) assay for the detection of Legionella sp. and L. pneumophila in cooling tower samples. We used culture method as a gold standard.Methods: Optimization of dPCR method was performed to obtain an assay with high sensitivity and specifi city. The optimized method was used to detect Legionella sp. dan L. pneumophila in 9 samples obtained from 9 buildings in Jakarta. For culture method, the bacteria were grown or isolated on selective growth factor supplemented-buffered charcoal yeast extract (BCYE) media.Results: Of 9 samples tested by dPCR assay, 6 were positive for Legionella species,1 was positive for L. pneumophila, and 2 showed negative results. For the same samples, no Legionella sp. was detected by the culture method.Conclusion: dPCR assay was much more sensitive than the culture method and was potentially used as a rapid, specifi c and sensitive test for routine detection of Legionella sp. dan for L. pneumophila in water samples. (Med J Indones 2010; 19:223-7)Keywords: BCYE media, mip gene, 16S-rRNA gene
Co-Authors Adin H Kurniawan Adin Hakim Kurniawan Ahniar, Nur Hasanah Andi Yasmon Anis Anis Budiman Bela Cartika, Harpolia Desi Suryani Emini Emini Erwin Erwin Faradilla Safitri, Faradilla Fatwa Hasbi Nanang Kurnia Nanang Kurnia Nanang Kurnia Achmadi Nanda Puspita Purnama Fajri Rini Widiyastuti Riza Alfian Ruth Elenora Kristanty Surahman Surahman Surahman Surahman Susanto, Yugo Syarifah Miftahul El Jannah Ulya Safrina