Garuda - Garba Rujukan Digital

Kualitas Semen dengan Berbagai Formulasi Pengencer Dasar Air Kelapa Hijau Selama Simpan Dingin pada Sapi Madura Aulia Puspita Anugra Yekti; Jois Harsah; Muchamad Luthfi; Muhammad Dikman; Asri Nurul Huda; Kuswati Kuswati; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

ABSTRAKInseminasi Buatan dengan menggunakan semen cair digunakan untuk daerah yang sulit nitrogen cair dan mempunyai kualitas yang lebih baik dari pada semen beku. Tujuan penelitian ini adalah untuk mengetahui kualitas berbagai bahan pengencer dasar air kelapa penyimpanan dingin 2-5°C.Penelitian ini dilaksanakan di Loka Penelitian Sapi Potong Grati, Pasuruan. Semen yang digunakan berasal dari sapi madura sebanyak dua ekor, berumur 5 dan 3 tahun dan berat badan yaitu 397 kg dan 360,5 kg. Sapi madura ditampung seminggu 2 kali dengan motilitas > 70% , sedangkan air kelapa yang digunakan adalah air kelapa hijau yang masih muda. Pengenceran semen cair dibagi menjadi 4 yaitu P0 (CEP-3 + 20% kuning telur) sebagai kontrol, P1 (air kelapa hijau +20% kuning telur), P2 (P1 + 0,4% putih telur + 1% fruktosa) dan P3 (P1 + 0,4% putih telur kuning telur +2% fruktosa). Data dianalisis menggunakan uji Pearson’s Chi Square dan Uji Deskriptif. Hasil penelitian menunjukkan motilitas spermatozoa sesuai standar SNI yaitu motilitas> 40% pada pengencer CEP-3 dapat disimpan selama hari ke-8 (40,50±6,43%) sedangkan pada pengencer dasar air kelapa hijau pada P1, P2 dan P3 tidak menunjukkan perbedaan yang nyata (P>0,05) selama disimpan 6 hari yaitu 40,50±10,12%, 38,00±4,22%, 40,00±8,50%. Abnormalitas dari semua perlakuan menunjukan nilai <20%. Viabilitas didapatkan nilai tertinggi pada perlakuan P0(89,58±2,16%) kemudian P1(89,39±3,79%), P2(88,62±4,59%) dan P3(87,93±4,41%).Kata kunci: CEP-3, semen cair, sapi madura, simpan dingin, air kelapa hijau ABSTRACTArtificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%). Keywords:CEP-3, liquid semen, madura bull, cool storage, green coconut water ABSTRACT Artificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%).

Pengaruh Sumber Daya Manusia terhadap Aksesibilitas Sumber Daya Usaha Ternak Sapi Potong Rakyat Amam Amam; Hidayat Bambang Setyawan; Muhammad Wildan Jadmiko; Pradiptya Ayu Harsita; Supardi Rusdiana; Muchamad Luthfi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 1 (2021): JITRO, Januari
Publisher : Universitas Halu Oleo

ABSTRAK Sumber Daya Manusia (SDM) peternak merupakan salah satu faktor kunci keberhasilan usaha ternak serta berperan penting terhadap perkembangan dan keberlanjutan usaha ternak. Teori menyebutkan bahwa tinggi dan rendahnya kualitas SDM dapat mempengaruhi aksesibilitas sumber daya pada suatu sistem bisnis. Tujuan penelitian ialah mengkaji pengaruh SDM peternak terhadap aksesibilitas sumber daya usaha ternak sapi potong. Lokasi penelitian ialah Desa Purnama, Kecamatan Tegalampel, Kabupaten Bondowoso. Desa Purnama sebagai salah satu Desa Binaan Universitas Jember untuk pengembangan usaha ternak sapi potong di Kabupaten Bondowoso. Variabel penelitian meliputi SDM peternak (X), sumber daya finansial (Y1), sumber daya teknologi (Y2), dan sumber daya fisik (Y3). Pengambilan data dilakukan dengan metode Focus Group Discussion (FGD), observasi, dan survei dengan menggunakan wawancara dan pengisian kuisioner berskala likert. Analisis data dilakukan dengan metode Partial Least Square (PLS). Hasil penelitian menunjukkan bahwa SDM peternak berpengaruh positif terhadap aksesibilitas peternak pada sumber daya finansial, teknologi, dan fisik. Kesimpulan penelitian menunjukkan bahwa sumber daya finansial, teknologi, dan fisik dipengaruhi secara signifikan oleh kualitas SDM peternak masing-masing sebesar 0,359; 0,431; dan 0,372.Kata kunci: finansial, sapi potong rakyat, sumber daya, teknologi, fisikThe Effect of Human Resources on the Resources Accessibility of Beef Cattle Farming Business ABSTRACT Human Resources (HR) farmers are one of the key factors in the success of livestock farming businesses and play an important role in the development and sustainability of livestock farming businesses. The theory states that the high and low quality of HR can affect the accessibility of resources in a business system. The research objective was to examine the influence of farmer’s HR on the accessibility of beef cattle farming business resources. The research location is Purnama Village, Tegalampel District, Bondowoso Regency. Purnama Village is one of the Fostered Villages of Universitas Jember for the development of beef cattle farming business in Bondowoso Regency. The research variables are farmer HR (X), financial resources (Y1), technological resources (Y2), and physical resources (Y3). Data were collected using Focus Group Discussion (FGD) method, observation, and surveys using interviews and questionnaires on a Likert scale. Data analysis was performed using the Partial Least Square (PLS) method. The results showed that the farmers' HR had a positive effect on the accessibility of farmers to financial, technological and physical resources. The conclusion of this research shows that financial, technological, and physical resources are significantly affected by the quality of the farmers' HR by 0.359; 0.431; and 0.372 respectively.Keywords: financial, beef cattle, resources, technological, physical

Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin Dedi Muhammad; Nurul Isnaini; Kuswati Kuswati; Aulia Puspita Anugra Yekti; Muchamad Luthfi; Lukman Affandhy Sunarto; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

ABSTRAKPengencer dasar air kelapa merupakan solusi dari sulit dan mahalnya harga pengadaan bahan baku pembuatan pengencer. Tujuan dari penelitian ini adalah untuk mengetahui kapasitasi spermatozoa sapi Peranakan Ongole dalam berbagai formulasi pengencer air kelapa selama simpan dingin pada suhu 4-5oC. Metodologi yang digunakan adalah eksperimantal laboratorium menggunakan rancangan acak kelompok (RAK). Terdapat tiga perlakuan yaitu, P1 = Pengencer air kelapa + 20% kuning telur, P2 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 1000 mg/l. P3 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 2000 mg/l, dengan 10 ulangan. Hasil menunjukkan bahwa tidak terdapat perbedaan yang signifikan pada persentase spermatozoa belum kapasitasi, terkapasitasi dan telah rekasi akrosom antara perlakuan formulasi pengencer air kelapa yang berbeda (P1, P2, P3) selama peyimpana suhu 2-5oC. Pengencer berbasis air kelapa mampu mempertahankan kualitas akrosom diatas 50% penyimpanan hari ke lima.Kata kunci : air kelapa, kapasitasi, reaksi akrosom, semen cairABSTRACTCoconut water extender is the solution to the difficulty and high cots of semen diluents. The objective of this study were to examine the capacitation of Ongole Crossbreed sperm in various formulations of coconut water extender during storage in 4-5 oC. The study method used laboratory experimental. The experimental design was Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are three treatments in this study (P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were not significant differences in percentage of sperm uncapacitation, sperm capacitaion and sperm acrosom reaction between various formulations of coconut water diluents during chilled storage at 4-5 oC. Coconut water diluents are able to maintain quality of sperm acrosom up to day 5th with values above 50%.Keywords: acrosom reaction, capacitation, coconut water, liquid semen

Kualitas Semen Beku Sexing Sapi Peranakan Ongole Menggunakan Volume Semen Awal Yang Berbeda Irvan Mardi; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Muchamad Luthfi; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 3 (2020): JITRO, September
Publisher : Universitas Halu Oleo

ABSTRAKInseminasi buatan dengan menggunakan semen sexing diharapkan menghasilkan pedet dengan jenis kelamin sesuai harapan. Tujuan dari penelitian ini adalah untuk mengetahui kualitas, proporsi, dan jumlah produksi straw sexing menggunakan metode sentrifugasi gradien densitas percoll dengan volume awal semen yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong, Kecamatan Grati, Kabupaten Pasuruan dan Laboratorium Reproduksi Ternak, Fakultas Peternakan Universitas Brawijaya, Malang. Materi yang digunakan adalah semen sapi peranakan ongole berumur berkisar lima tahun dan bobot badan berkisar 700 kg sebanyak tiga ekor, motilitas massa ≥2+ dan motilitas individu ≥70%. Metode yang digunakan adalah eksperimental dengan tiga perlakuan volume awal saat sexing, yaitu 1 (P1); 1,5 (P2); dan 2 (P3) ml dengan ulangan 11 kali (ulangan berfungsi sebagai kelompok). Data dianalisa menggunakan Rancangan Acak Kelompok (RAK). Hasil penelitian menunjukkan bahwa perbedaan volume awal semen tidak berpengaruh (menurun) terhadap motilitas, viabilitas, abnormalitas, konsentrasi, total spermatozoa motil, recovery rate dan proporsi spermatozoa (P>0,05). Pengaruh yang sangat nyata (meningkat) terhadap jumlah produksi straw (P<0,01). Ulangan penelitian ini memberikan pengaruh yang sangat nyata (meningkat) terhadap kualitas (motilitas, konsentrasi, viabilitas, abnormalitas, total spermatozoa motil, RR, proporsi dan jumlah straw) dan proporsi spermatozoa X dan Y (P<0,01). Total spermatozoa motil setiap perlakuan telah memenuhi nilai harapan (10 juta/straw). Proporsi spermatozoa X dan Y telah memenuhi nilai harapan (80%:20%).Kata Kunci: kualitas, proporsi, semen beku sexing, strawABSTRACTArtificial insemination using sexing semen is expected to produce calves with the expected sex. The aim of this study was to determine the quality, proportion, and quantity of sexing semen production using the percoll density gradient centrifugation method with different initial semen volumes. The research was conducted at the Beef Cattle Research, Grati District, Pasuruan Regency, East Java Province, Indonesia, and the Animal Reproduction Laboratory, Faculty of Animal Science, University of Brawijaya, Malang, East Java Province, Indonesia. The material used was semen from three Ongole crossbred bull aged around five years and the bodyweight of around 700 kg, mass motility of ≥2+, and individual motility ≥70%. The method used was experimental with three initial volume treatments during sexing, namely 1 (P1); 1.5 (P2), and 2 (P3) ml with 11 replications (replications function as groups). The data were analyzed using a randomized block design (RBD). The results showed that the treatment of differences in initial semen volume did not affect motility, viability, abnormalities, concentration, total motile sperm, recovery rate, and proportion of sperm (P>0.05). On the other hand, the difference in the initial volume of semen had a very significant effect (increased) on the amount of frozen semen production (P<0.01). Repeated research also had a very significant effect (increased) on the semen quality (motility, concentration, viability, abnormality, total sperm motility, recovery rate proportion, and straw production) and the proportion of spermatozoa X and Y (P<0.01). The total motile sperm for each treatment had met the expected value (10 million/straw). Proportions of spermatozoa X and Y have met the expected value (80%: 20%).Keywords: proportion, quality, sexing frozen semen, straw.

Title

Found 4 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 4 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 4 Documents
Search