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All Journal Jurnal Medika Veterinaria Jurnal Ilmiah Mahasiswa Veteriner
Maryulia Dewi
Universitas Syiah Kuala
Health Professions Veterinary

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17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate Maryulia Dewi; Darmawi Darmawi; T. Zahrial Helmi; Erina Erina; Basri Gani; Eliawardani Eliawardani; Azhar Azhar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.621 KB) | DOI: 10.21157/j.med.vet..v13i1.8884

Abstract

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.
18. Phytochemistry and Antibacterial Activity Test of Ethanol Extracts of Soursop flower (Annona muricata L.) against Salmonella enteritidis Zuraidawati Zuraidawati; Maryulia Dewi; Darmawi Darmawi; Sugito Sugito; Abdullah Hamzah; Winaruddin Winaruddin
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.438 KB) | DOI: 10.21157/j.med.vet..v13i1.8885

Abstract

           The purpose of this research was to identified the compounds in ethanol extract of soursop flower and determined the antibacterial activity of the soursop flower extract on Salmonella enteritidis. The phytochemistry screening was done to identify the secondary metabolite compounds of this extract. The concentrations used of ethanol extract of soursop flower were 10%, 20%, 30%, 40%, 50%, with ampicillin 10 μg/disc as the positive control and 10% Dimethyl Sulfoxide (DMSO) as the negative control. The antibacterial activity test of soursop flower ethanol extract against Salmonella enteritidis was held in vitro using the paper disc diffusion method by measuring the diameter of the inhibition zone. The phytochemistry screening showed that the ethanol extract of soursop flower had secondary metabolites such as alkaloids, phenolic, and flavonoid. The result of the antibacterial activity test showed that there was no inhibition zone (bright zone) at various concentrations. So, it can be concluded that soursop flower ethanol extract (Annona muricata L.) contained the secondary metabolites such as alkaloids, phenolic, and flavonoid, and also this extract had no antibacterial activity against Salmonella enteritidis
ISOLASI DAN IDENTIFIKASI BAKTERI GRAM NEGATIF PADA AMBING KAMBING PERANAKAN ETAWA (PE) (Isolation and Identification of Gram Negative Bacteria on the Udder of Etawa Crossbred (PE) Goat) Septian Tri Mulyana Ginting; Teuku Zahrial Helmi; Darmawi Darmawi; Maryulia Dewi; Erina Erina; Razali Daud; Hennivanda Hennivanda
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.36 KB) | DOI: 10.21157/jim vet..v2i3.8206

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Gram negatif pada ambing kambing peranakan etawa (PE). Sampel yang digunakan pada penelitian ini terdiri dari swab ambing dari 5 ekor kambing PE. Sampel dibiakkan pada media nutrient broth lalu diinkubasikan pada suhu 37°C selama 24 jam. Isolasi dan identifikasi dilakukan dengan metode Carter yang meliputi pengamatan morfologi koloni, pewarnaan Gram, penanaman pada media diferensial dan selektif serta uji biokimia. Hasil isolasi dan identifikasi pada 5 sampel swab ambing kambing PE didapatkan dua koloni bakteri Gram negatif yaitu Escherichia coli dan bakteri X yang belum teridentifikasi melalui uji-uji yang dilakukan. Berdasarkan hasil tersebut dapat disimpulkan bahwa bakteri Gram negatif pada ambing kambing PE adalah Escherichia coli dan bakteri X yang belum teridentifikasi.Kata kunci: kambing PE, ambing, bakteri Gram negatif. ABSTRACTThis study aimed to isolate and identify Gram-negative bacteria on udder of etawa crossbred (PE) goat. Swab samples were obtained from 5 PE goats udder. Swab samples were cultured in nutrient broth media and incubated at 37°C for 24 hours. Isolation and identification were performed by Carter method, followed by observation of colony morphology, Gram staining, cultured on differential and selective media, and biochemical tests were performed. The result showed that there were two colonies of Gram negative bacteria Escherichia coli and the other colony has not been identified yet with this tests. Therefore it can be concluded that the Gram-negative bacteria found on udder of PE goat were Escherichia coli and bacteria X that has not been identified by using this method.Keywords:etawa crossbred goat, udder, Gram-negative bacteria.
Co-Authors . Darmawi Abdullah Hamzah Azhar Azhar Basri Gani Eliawardani Eliawardani Erina Erina HENNIVANDA HENNIVANDA Razali Daud Septian Tri Mulyana Ginting Sugito Sugito T. Zahrial Helmi Teuku Zahrial Helmi Winaruddin Winaruddin Zuraidawati Zuraidawati