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All Journal Jurnal Sain Veteriner Jurnal Veteriner
Alfarisa Nururrozi
Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Veterinary

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Perkembangan Resistensi Escherichia coli terhadap Oksitetrasiklin Soedarmanto Indarjulianto; Alfarisa Nururrozi; Yanuartono Yanuartono; Rusmihayati Rusmihayati; Sitarina Widyarini; Puspa Wikan Sari; Vembriarto Jati Pramono
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5384.056 KB) | DOI: 10.22146/jsv.38453

Abstract

Antimicrobial resistance is amongst the primary concern in the field of veterinary medicine worldwide. The research was conducted to evaluate the resistance of Escherichia coli due to inappropriate antibiotic treatment. Escherichia coli in this research was cultured in vitro using 5-ml Tryptone Soya Broth (TSB), which was mixed with an antibiotic, then was incubated at 37 0C for four days (stage I). Subsequently, a part of the culture was transferred  into another 5-ml TSB and incubated at 37 0C for four days (stage II). This procedure was undertaken continuously for stage III, IV, V,and VI (until day 24). Antibiotics in this research was oxytetracycline with three various doses, which are lower dose (1 mg/ml), normal dose (2 mg/ml), and higher dose (8 mg/ml). The development of the antrimocobial resistance was evaluated every four days, using disk diffusion method and the data were analysed descriptively. The results showed that the normal and higher doses of oxytetracycline has the same rate (day 16) in causing E. coli resistant to oxytetracycline. Therefore, the treatment of oxytetracycline with a normal and higher doses continuously could accelerate the emergence of antimicrobial resistance in Escherichia coli.
Diagnosis Feline Panleukopenia Berdasar Total Leukosit dan Uji Feline Parvovirus-Antigen pada Kucing-Kucing Diare Hary Purnamaningsih; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Alfarisa Nururrozi; Irkham Widiyono; Slamet Rahardjo; Sri Hartati; Rusmihayati Rusmihayati
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.718 KB) | DOI: 10.19087/jveteriner.2022.23.1.36

Abstract

Feline panleukopenia (FPL) is one of the most common disease that causes diarrheal and leukopenia in cats. The purpose of this study was to diagnose FPL based on total leukocyte and detection of feline parvovirus antigen (FPV-Ag) in diarrhea cats. This study used blood samples and feces swabs from 21 cats that showed symptoms of diarrhea. Total leukocytes are calculated from blood samples and the presence of FPV antigens is detected from feces swabs using FPV-Ag-kit. The suitability of the FPL diagnosis based on leukopenia and a positive reaction to the FPV-Ag test was compared using the Cohen’s Kappa suitability test. The results showed that 14 out of 21 cats (66.7%) had leukopenia and 7 of 21 cats (33.3%) had normal total leukocytes. Results of FPV-Ag test showed that 11 of 21 cats (52%) were positive and 10 of 21 cats (48%) were negative. The both test has a good agreement with a 0.71 conformity value. Based on this study it was concluded that the diagnosis of FPL based on total leukocyte and FPV-Ag have a good agreement, so that both tests could be used as a basis for FPL diagnosis.
Co-Authors Hary Purnamaningsih Irkham Widiyono Puspa Wikan Sari Rusmihayati Rusmihayati Sitarina Widyarini Slamet Rahardjo Soedarmanto Indarjulianto Sri Hartati Vembriarto Jati Pramono Yanuartono Yanuartono