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SELEKSI INDUK JANTAN CUPANG ALAM B CHANNOIDES DAN PENENTUAN UMUR PANEN LARVA METODE PENGOCOKAN SERTA EFEKTIFITASNYA Agus Priyadi; Asep Permana; Ahmad Musa; Bastiar Nur; Sawung Cindelaras; Sulasy Rohmy; Siti Zuhriyyah Musthofa
Jurnal Akuakultur Rawa Indonesia Vol 9, No 2 (2021): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v9i2.15478

Abstract

Snakehead (Betta channoides) is a native and endemic fish from East Kalimantan. It has a beautiful and attractive color as an ornamental fish commodity. This fish is incubating her eggs in its mouth or a mouthbrooder. The important thing in spawning is male brood selection, it is related to eggs incubating and also proper harvests time and technique in order to maximize the result. The aim of this research is to determine the criteria for good male broodstock used in spawning, proper time to harvest the larvae and the effectiveness of shaking technique to harvest the larvae. There are two criteria selecting male broodstock, 1. Mouth shape and 2. Body coloration. Determination of harvest time was carried by harvesting larvae 4th, 8th and 12th day after spawning. The effectiveness of shaking technique was seen by comparing with broodstock natural larva released. The parameters that observed in this research were the shape of male broodstock, male body color, the number of harvested larvae and harvesting time between shaking technique and naturally released by male. The result showed that selection of male snakehead betta good for spawning was the male broodstock with wider mouth shape and have width of 0.5cm also with not too bright red body coloration. The proper harvesting time for maximum yield was the 8th day after spawning. The shaking technique in harvesting is more effective in harvest time, number of larvae, survival rate of larvae and its simplicity comparing to natural released by broodstock.  Keywords: selection, male broodstock, larva harvest, shaking, B. channoides
PEMIJAHAN ALAMI ARWANA SILVER (Osteoglossum bicirrhosum) DALAM BAK TERKONTROL Agus Priyadi; Rendy Ginanjar; Chumaidi Chumaidi; Wartono Hadie
Jurnal Riset Akuakultur Vol 5, No 3 (2010): (Desember 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.396 KB) | DOI: 10.15578/jra.5.3.2010.345-350

Abstract

Ikan arwana silver (Osteoglossum bicirrhosum) adalah ikan hias introduksi berasal dari Brazil yang sudah menjadi ikan hias potensial. Tujuan penelitian ini ialah untuk mengetahui teknik pemijahan secara alami ikan arwana silver dalam bak terkontrol. Induk arwana jantan dan betina yang sudah diberi penanda (tagging) sebanyak 12 ekor diadaptasikan dalam bak beton (3 m x 2 m x 1 m) yang diletakkan di dalam ruangan yang dilengkapi dengan instalasi aerasi. Pakan diberikan berupa percil (50 g) dan ikan selar (360 g) dua hari sekali. Setelah 10 bulan ikan arwana mencapai panjang berkisar 59-64 cm dan bobot berkisar 1.208,5-1.849,5 g. Semua ikan arwana (11 ekor) dipindah ke luar ruangan, dipelihara dalam bak beton (5 m x 2 m x 1.2 m) dengan media air yang diputar menggunakan pompa dan bak pemeliharaan ditutup dengan jala. Pakan diberikan sama seperti saat adaptasi. Pengamatan keberhasilan pemijahan ikan dilakukan setiap saat dengan mengetahui ada tidaknya telur yang dierami dalam mulut arwana jantan. Pengeluaran larva dari dalam mulut induk jantan dilakukan setelah telur dierami selama 1-1,5 bulan. Hasil penelitian menunjukkan bahwa setelah 8 bulan pemeliharaan di luar ruangan, arwana silver jantan terlihat mengerami telur dalam mulutnya pertama kali dan menghasilkan larva sebanyak 17 ekor, kemudian berturut-turut menghasilkan larva setiap bulan pada bulan ke-22, 23, dan 24 berkisar 25-107 ekor/induk/bulan. Silver arowana (Osteoglossum bicirrhosum) is an ornamental fish introduced from Brazil and has been popular among fish collectors. The objective of this research was to determine the optimal spawning technique of silver arowana in controlled tank. Tagged male and female of arowana broodstock were adapted in 3 m x 2 m x 1 m indoor concrete tank with stocking density of 12 individual/tank. Lift frogs (50 g) and raw fish (360 g) were given as feed once per two days. After ten months of rearing, the total length of silver arowana broodstock varied from 59 to 64 cm and body weight ranged from 1,208.5 to 1,849.5 g. Eleven individuals of silver arowana broodstocks were stocked in 5 m x 2 m x 1.2 m out door concrete tank in which the water media was recirculated using water pump and rearing tank were covered with net. The feeds given were the same as during the adaptation process. Observation of breeding was done regularly by observing whether the eggs were already in the male broodstock mouth or not. The process to collect the larvae out of the male broodstock mouth was done after the eggs were incubated in its mouth for about 1.0-1.5 month. Result showed that after 8 months of rearing in outdoor tank, male silver arowanas were seen carrying eggs in their mouth for the first time and produce 17 larvae. On average, as many as 25-107 larvae were obtained per broodstock starting from 22nd, 23nd, and 24nd of rearing period.
PENYISIPAN GEN WARNA PADA IKAN Carassius auratus MENGGUNAKAN METODE ELEKTROFORASI DALAM UPAYA MENINGKATKAN KUALITAS IKAN HIAS Wartono Hadie; Eni Kusrini; Agus Priyadi; Alimuddin Alimuddin
Jurnal Riset Akuakultur Vol 5, No 3 (2010): (Desember 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.653 KB) | DOI: 10.15578/jra.5.3.2010.335-343

Abstract

Tujuan penelitian ini adalah untuk mendapatkan teknik rekayasa penyisipan gen warna pada ikan hias dan pengetahuan tentang pola pewarisannya yang akan dapat membantu perbaikan mutu ikan. Metode yang diterapkan adalah elektroforasi. Ikan hias yang digunakan dalam penelitian adalah ikan komet (Carassius auratus) sedangkan gen pemendar yang digunakan adalah GFP (green fluorescent protein) dengan konstruksi DNA yang digunakan berbentuk plasmid yang dikontrol oleh promoter b-aktin dari ikan Japanese flounder (ikan sebelah) dengan panjang fragmen pKer-GFP 6,0 kb. Sintasan dan keberadaan gen GFP diamati mulai dari telur menetas. Ekspresi gen dapat diamati setelah fase terbentuknya sirip dan dilakukan secara deskriptif (performa) dan PCR. Hasil yang diperoleh gen GFP terekspresi mulai pembentukan sirip dan hasil cek PCR semua ketiga konsentrasi DNA yang dicobakan mempunyai ekspresi yang sama. Untuk dapat mengetahui ekspresi gen GFP sampai pada F0 masih menunggu ikan dewasa dan matang gonad.This research was aimed to obtain a method for introduction of exogenous gene of green flourescent protein (GFP) in order to improve the ornamental fish color appearance. The method used were electroporation. The Carassius auratus were used in this research. The construct of flourescent gene of pKer-GFP as DNA construct of plasmid controlled by b-actin of Japanese flounder with pKer-GFP 6.0 kb in length. Survival rate and gene expression of GFP assessed right after the eggs hatched. Gene expression was observed using PCR product and direct observation. The result show that the expression of GFP from all of three treatments were observed but not significantly different. This expression however should be wait until the fish mature.