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All Journal Berkala Perikanan Terubuk JBIO: jurnal biosains (the journal of biosciences) Saintia Biologi Microbiology Indonesia
It Jamilah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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AKTIFITAS ANTI BAKTERI SPESIES ASTERIAS FORBESII TERHADAP BEBERAPA JENIS BAKTERI PATOGEN Siti Juariah; Dwi Suryanto; It Jamilah
Berkala Perikanan Terubuk Vol 42, No 2 (2014): Juli 2014
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.043 KB) | DOI: 10.31258/terubuk.42.2.%p

Abstract

ABSTRACTTo avoid contamination of bacteria pathogens natural and safe antibacterial agent is needed. The alternative sources of antibacterial compound is derived from sea star. In this extraction study of sea star activity showed that activity of the methanol from extract of starfish have the highest inhibition against for several bacterial pathogens compared to that of ethyl acetate and n-hexane extract with inhibition zone of 9.5 mm, 8.5 mm, 10.0 mm, 11.0 mm in S. aureus, B. subtilis, P. auroginosa and E. coli respectively. It showed that of TLC preparatif triterpenoid was capable inhibition more in Gram negative bacteria (Asterias forbesii) has been caried out to obtain antibacterial compounds. To determine bioactive components in the extract of sea star chemical test has been conducted. Toxicity of secondary metabolites was determind using method of Brine Shrimp Lethality Test (BSLT). Assay of antibacterial activity of starfish extract was conducted against four pathogenic bacterial strains, two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), and two Gram-negative bacteria (Pseudomonas auroginosa and Escherichia coli). The result showed that the methanol extract contained alkaloid, terpenoids, saponins and flavonoids, while the extract of n-hexane and ethyl acetate only contain saponins and flavonoids. BSLT test showed that LC50 of extract of n-hexane, ethyl acetate and methanol were 1412,54 ppm, 13182,57 ppm and 63,10 ppm respectively. Antibacterial (E. coli and P. auroginosa) bacteria compared with Gram positive (S. aureus and B. subtilis) .Keywords: Asterias forbesii, Staphylococcus aureus, Bacillus subtilis, Pseudomonas auroginosa, Escherichia coli, antibacterial compound
POTENSI EKSTRAK UMBI BAWANG LOKIO (Allium chinense G. Don) DALAM MENGHAMBAT Escherichia coli DAN MENINGKATKAN MASA SIMPAN IKAN NILA (Oreochromis niloticus L.) Grace Emalia Masniari Lumbantoruan; Nunuk Priyani; It Jamilah
JBIO: jurnal biosains (the journal of biosciences) Vol 2, No 3 (2016): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v2i3.4957

Abstract

Bawang Lokio (Allium chinense G. Don) banyak tumbuh di Sumatera Utara, Indonesia, sehingga disebut “Bawang Batak” oleh suku Batak. Kandungan senyawa alami dari tanaman ini dalam pengawetan makanan segar belum banyak dilaporkan. Penelitian ini bertujuan untuk menentukan konsentrasi hambat minimum dari ekstrak etanol umbi bawang Lokio dalam menghambat bakteri Escherichia coli yang diisolasi dari ikan Nila dan meningkatkan masa simpan ikan Nila. Aktivitas antimikroba diuji menggunakan metode difusi cakram dengan konsentrasi 0, 7.5, 10, 20, 30, 40 dan 50% (b/v). Untuk uji pengawetan ikan segar, ikan Nila yang telah disiangi, direndam dalam ekstrak etanol umbi bawang Lokio konsentrasi 100%, disimpan selama 36 jam pada suhu ambient (27 °C) dan kulkas (4 °C) dengan waktu pengamatan setiap interval 6 jam. Parameter pengamatan ialah perubahan organoleptik, Angka Lempeng Total (ALT) bakteri dan Total Volatile Base Nitrogen (TVBN). Konsentrasi Hambat Minimum (KHM) dari ekstrak etanol umbi bawang Lokio adalah 10% dengan kategori sedang terhadap E. coli. Berdasarkan Standar Nasional Indonesia (SNI), nilai organoleptik ikan Nila dapat diterima hingga penyimpanan jam ke-30, sementara nilai ALT dan TVBN sampai jam ke-18. Hal ini berbeda secara signifikan dengan kontrol negatif yang memberikan kelayakan ikan hanya sampai 6 jam. Perbedaan aktivitas senyawa antimikroba pada konsentrasi yang berbeda dan suhu penyimpanan menjadi penyebab turunnya mutu ikan. Dapat disimpulkan bahwa ekstrak etanol umbi bawang Lokio menghambat E. coli pada konsentrasi hambat minimum 10% serta masa simpan ikan Nila maksimal adalah 18 jam pada suhu ambient.   Kata Kunci: Bawang Lokio, ikan Nila, masa simpan, Sumatera Utara.
POTENSI TANAMAN ORNAMENTAL (Aglaonema sp., Dieffenbachia sp., dan Spathiphyllum sp.) DALAM MENURUNKAN JUMLAH BIOAEROSOL Sisca Teresia; It Jamilah; Nunuk Priyani
JBIO: jurnal biosains (the journal of biosciences) Vol 2, No 3 (2016): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v2i3.4958

Abstract

Bioaerosol adalah partikel debu yang terdiri atas bakteri dan jamur beserta spora lainnya yang mampu bertahan hidup dalam ruangan ketika tingkat suhu dan kelembaban yang memadai. Keberadaannya diruangan dalam batas tertentu tidak berbahaya, namun sewaktu-waktu dapat mengganggu kesehatan. Penelitian ini bertujuan untuk mengetahui pengaruh tanaman ornamental Aglaonema sp., Dieffenbanchia sp. dan Spathiphyllum sp. dalam mengurangi jumlah koloni bakteri dan jamur dalam ruangan. Isolasi mikrob bioaerosol dilakukan dengan metode air sampling dengan tiga kali ulangan sebelum dan setelah tiga jenis tanaman diletakkan pada tiga ruangan kelas yang berbeda. Tanaman Aglaonema sp. memiliki potensi paling efektif dalam menurunkan jumlah bakteri aerosol dari minggu pertama hingga minggu ke tiga, sedangkan untuk koloni jamur tidak menunjukkan adanya penurunan. Jumlah koloni bioaerosol pada control dalam ruangan masih sesuai dengan baku mutu keputusan Menteri Kesehatan RI tahun 2002. Jenis bakteri yang ditemukan seperti Streptococcus, Staphylococcus, Bacillus, Pseudomonas dan Shigella, sedangkan jenis jamur yang ditemukan seperti Aspergillus, Penicillium, dan Neurospora.   Kata Kunci: Bioaerosol, Ruang kelas, Aglaonema sp., Spathiphyllum sp.,  Dieffenbanchia sp.
VIABILITAS DAN KERIAP Bacillus sp. BK17 DAN Enterobacter sp. BK15 PADA SUMBER KARBON DAN NITROGEN YANG BERBEDA Sirma Novita Nasrah; Dwi Suryanto; It Jamilah
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.855 KB)

Abstract

To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x108 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x108 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, agar molases-urea medium with 2% agar is suitable medium for swarming ability for both bacteria. Keywords : Bacillus, C and N-source, Enterobacter, swarming, viability
PENGENDALIAN SEL BIOFILM BAKTERI PATOGEN OPORTUNISTIK DENGAN PANAS DAN KLORIN Yusnita Wahyuni Silitonga; It Jamilah; Dwi Suryanto
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.282 KB)

Abstract

Opportunistic pathogenic bacteria are bacteria that are naturally not present in an environment that, but it dues to contamination of the environment by human waste. From previous research it was found E. coli, Staphylococcus sp. and Salmonella were opportunistic pathogens of the shrimp aquaculture (Percut, Pantai Labu, Pantai Cermin). The aims of this study is to know the ability of these bacteria to form biofilms as well as its control using chlorine and heat. In order to test the ability of the bacteria to form biofilms, the stainless steel have been soaked in SWC media for 1, 3, 6 days. E. coli, Salmonella, and Staphylococcus sp. may form biofilms on incubation of the 1st day but the highest growth was on the 6th day of incubation by E. coli with a 6,35 x 104 CFU/SS, whereas of the lowest biofilm number was found in Salmonella with a 0,28 x 104 CFU/SS. The number of biofilm cells grow in line with a length of incubation. In this research, the most effective concentration of chlorine to kill biofilm cell was  225 ppm for 2 minutes and heat was 100 0C for 5 minutes. The higher the concentration of chlorine and the temperature given more effective to kill the bacteria.   Keyword: biofilm, chlorine, heat, opportunistic, stainless steel
SELEKSI BAKTERI PENAMBAT NITROGEN DAN PENGHASIL HORMON IAA (Indole Acetic Acid) DARI RIZOSFER TANAH PERKEBUNAN KEDELAI (Glycine max L.) Ratna Sari Tarigan; It Jamilah; Elimasni D
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Rizosphere bacteria can be used to fix nitrogen and  produce IAA (Indol Acetic Acid) as biofertilizer to support plant growth. The ability of rhizosphere bacteria can be improved using biotechnology. The aim of this study is to select the most potential nitrogen fixing and IAA producing bacteria. Nitrogen fixing and IAA producing bacteria were isolated using JNFB and Luria Bertani + L-tryptofan medium respectively. The ability of nitrogen fixing bacteria were tested by ARA method, while the ability of IAA producing bacteria were investigated by spectrophotometer technique of 535 nm. The highest IAA concentration was produced by isolate I3 which was 33.3 ppm and the highest concentration of nitrogen was yielded by isolate N3 which was 29.93 ppm. Both N3 isolate and I3 isolate potentially as biofertilizer known as PGPR (Plant Growth Promoting Rhizobacteria).   Keywords: nitrogen fixing bacteria, IAA producing bacteria, PGPR
Activity of Proteolytic and Amylolytic Enzymes from Bacillus spp. Isolated from Shrimp Ponds IT JAMILAH; ANJA MERYANDINI; IMAN RUSMANA; ANTONIUS SUWANTO; NISA RACHMANIA MUBARIK
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.373 KB) | DOI: 10.5454/mi.3.2.4

Abstract

Accumulation of feed excess in commercial shrimp ponds due to overfeeding could decrease water quality. Protein and starch are the primary components of shrimp feed. This study was conducted to characterize extracellular proteases and amylases of Bacillus spp. isolated from shrimp ponds. 72 proteolytic and amylolytic Bacillus spp. isolates were screened from shrimp ponds in Karawang, West Java. Ten isolates were selected for further characterization for their growth and ability to reduce total suspended solid generated from commercial shrimp feed. Bacillus sp. DA 5.2.3 and L5 showed excellent activity in reducing total suspended solid, by 37 and 30% respectively. Protease and a-amylase activities of Bacillus sp. DA 5.2.3 isolate were consistently higher than that of L5. Maximum total and specific protease activity of DA 5.2.3 isolate was 2.0 U mL-1 and 40.9 U mg-1 respectively, while the activities of the L5 isolate were 2.1 U mL-1 and 23.0 U mg-1 respectively. Based on its 16S rRNA gene sequences, Bacillus sp. DA 5.2.3 showed 99% similarity to Bacillus cereus XHJ-2-6. Bacillus sp. DA 5.2.3 could potentially be applied to maintain water quality by reducing total suspended solid in water columns of shrimp ponds.
Co-Authors Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R DWI SURYANTO Elimasni Grace Emalia Masniari Lumbantoruan Iman Rusmana NISA RACHMANIA MUBARIK Nunuk Priyani Nursal Ratna Sari Tarigan Sirma Novita Nasrah Sisca Teresia Siti Juariah Syafruddin Ilyas Yusnita Wahyuni Silitonga