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All Journal Berkala Perikanan Terubuk JBIO: jurnal biosains (the journal of biosciences) Saintia Biologi Microbiology Indonesia
It Jamilah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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Journal : Saintia Biologi

 1 
VIABILITAS DAN KERIAP Bacillus sp. BK17 DAN Enterobacter sp. BK15 PADA SUMBER KARBON DAN NITROGEN YANG BERBEDA Sirma Novita Nasrah; Dwi Suryanto; It Jamilah
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x108 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x108 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, agar molases-urea medium with 2% agar is suitable medium for swarming ability for both bacteria. Keywords : Bacillus, C and N-source, Enterobacter, swarming, viability
PENGENDALIAN SEL BIOFILM BAKTERI PATOGEN OPORTUNISTIK DENGAN PANAS DAN KLORIN Yusnita Wahyuni Silitonga; It Jamilah; Dwi Suryanto
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Opportunistic pathogenic bacteria are bacteria that are naturally not present in an environment that, but it dues to contamination of the environment by human waste. From previous research it was found E. coli, Staphylococcus sp. and Salmonella were opportunistic pathogens of the shrimp aquaculture (Percut, Pantai Labu, Pantai Cermin). The aims of this study is to know the ability of these bacteria to form biofilms as well as its control using chlorine and heat. In order to test the ability of the bacteria to form biofilms, the stainless steel have been soaked in SWC media for 1, 3, 6 days. E. coli, Salmonella, and Staphylococcus sp. may form biofilms on incubation of the 1st day but the highest growth was on the 6th day of incubation by E. coli with a 6,35 x 104 CFU/SS, whereas of the lowest biofilm number was found in Salmonella with a 0,28 x 104 CFU/SS. The number of biofilm cells grow in line with a length of incubation. In this research, the most effective concentration of chlorine to kill biofilm cell was  225 ppm for 2 minutes and heat was 100 0C for 5 minutes. The higher the concentration of chlorine and the temperature given more effective to kill the bacteria.   Keyword: biofilm, chlorine, heat, opportunistic, stainless steel
SELEKSI BAKTERI PENAMBAT NITROGEN DAN PENGHASIL HORMON IAA (Indole Acetic Acid) DARI RIZOSFER TANAH PERKEBUNAN KEDELAI (Glycine max L.) Ratna Sari Tarigan; It Jamilah; Elimasni D
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Rizosphere bacteria can be used to fix nitrogen and  produce IAA (Indol Acetic Acid) as biofertilizer to support plant growth. The ability of rhizosphere bacteria can be improved using biotechnology. The aim of this study is to select the most potential nitrogen fixing and IAA producing bacteria. Nitrogen fixing and IAA producing bacteria were isolated using JNFB and Luria Bertani + L-tryptofan medium respectively. The ability of nitrogen fixing bacteria were tested by ARA method, while the ability of IAA producing bacteria were investigated by spectrophotometer technique of 535 nm. The highest IAA concentration was produced by isolate I3 which was 33.3 ppm and the highest concentration of nitrogen was yielded by isolate N3 which was 29.93 ppm. Both N3 isolate and I3 isolate potentially as biofertilizer known as PGPR (Plant Growth Promoting Rhizobacteria).   Keywords: nitrogen fixing bacteria, IAA producing bacteria, PGPR
Co-Authors Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R DWI SURYANTO Elimasni Grace Emalia Masniari Lumbantoruan Iman Rusmana NISA RACHMANIA MUBARIK Nunuk Priyani Nursal Ratna Sari Tarigan Sirma Novita Nasrah Sisca Teresia Siti Juariah Syafruddin Ilyas Yusnita Wahyuni Silitonga