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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Kesehatan Tambusai
Ni Putu Senshi Septiasari
Universitas Bali Internasional
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES

 1 
Optimasi PCR dengan Penanda Daerah D-loop DNA Mitokondria untuk Metode Tes DNA Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 12 No 2 (2022): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2022.v12.i02.p03

Abstract

DNA testing is a molecular personal identification that can distinguish between individuals. One of the DNA markers used for PCR tests is mitochondrial DNA markers. Mitochondrial DNA is reported to have a polymorphic locus in the mitochondrial D-loop region. This study aims to determine the appropriate method to amplify mitochondrial DNA in the D-loop region for the DNA testing. A sample of 65 people was taken by purposive sampling method. Samples were extracted with phenol-chloroform and PCR with 2 types of PCR formulas, is formulas A and formulas B and the annealing temperatures used were 48oC, 52oC dan 56oC. The results showed that the optimal primary concentration for successful amplification was a Formulation B with primer concentration of 0,4 µM and condition variation II with annealing temperature of 52oC. A total of 62 Balinese people were successfully amplified with an amplicon length of 860 bp. Samples that are not successfully amplified are affected by the number of collected epithelial cells, the success of the DNA extraction process and null allele. So, from these results it can be concluded that the optimal conditions of DNA marker with a primer concentration of 0,4 µM and annealing temperature conditions of 52oC succeeded in amplifying the D-loop region of mitochondrial DNA.
Co-Authors Ayu Saka Laksmita W I GUSTI AGUNG AYU SATWIKHA DEWI - I Ketut Junitha I Ketut Junitha I Wayan Tanjung Aryasa Ni Made Sri Dwijastuti Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri Ni Putu Rahayu Artini Ni Putu Widayanti Ni Putu Widiantari Putri Ni Putu Risky Vidika A Widiantari Ni Putu Yulidia Iriani