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All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Biologi Udayana Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD)

I Ketut Junitha

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 1918405

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences

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Ragam Alel Mikrosatelit DNA Autosom pada Masyarakat Bali Aga Desa Sembiran Kabupaten Buleleng Bali Junitha , I Ketut; Bagus Alit, Ida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Berdasarkan data sejarah dan arkeologis, masyarakat Bali sekarang ini merupakan hasil perkembangan sejarah zaman pra-sejarah. Masyarakat Bali kuno yang masih memiliki tradisi zaman pra-sejarah disebut masyarakat Bali Aga atau Bali Mula. Pada umumnya masyarakat Bali Aga menempati daerah pegunungan seperti desa Tenganan, Terunyan, Sembiran dan Sidatapa, sedangkan masarakat Bali lainnya disebut masyarakat bali Dataran yan tinggal di kota-kota dan daerah pantai. Sebanyak delapan penanda genetik mikrosatelit autosom (D2S1338, D3S1358, D5S818, D7S820, D11S1984, D13S317, D16S539 dan D21S11) digunakan untuk menentukan variasi alel yang tersebar pada masyarakat desa Bali Aga Sembiran kabupaten Buleleng Bali untuk kepentingan forensik. Dalam penelitian ini ditemukan sebanyak 46 ragam alel dari 8 lokus yang digunakan, ragam alel perlokus berkisar antara 3 pada lokus D5S818 sampai 9 alel pada lokus D11S1984. Nilai kemampuan pembeda (power of discrimination/PD) tertinggi ditemukan pada lokus D11S1984 (0,9394) diikuti oleh D21S11(0,8922), D16S539 (0,8915), D13S317 (0,8602), D7S820 (0,8398), D3S1358 (0,8014), D2S1338 (0,5518) dan D5S818 (0,0143). Hasil ini menunjukkan bahwa lokus D5S818 tidak baik digunakan dalam analisis DNA untuk kepentingan forensik pada masyarakat Bali Aga desa Sembiran.

MUTATIONS IN 1700 BP FRAGMENT OF RPOB GENE OF MULTI-DRUG RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATE Yowani, Chandra; Sukardika, I K.; Mantik-Astawa, I N.; Junitha, I K.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 6 No 2 (2012): Indonesian Journal of Biomedical Sciences
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.737 KB)

This research aimed to amplify a 1700 bp fragment of rpoB gene of multidrug resistance M. tuberculosis (MDR-TB) isolate and determine types of mutation beyond the core region (hot-spot region). DNA sequencing studies indicate that more than 95% of rifampin-resistant M. tuberculosis strains have mutations within the 81-bp hot-spot region (codons 507 to 533) of the RNA polymerase ?-subunit (rpoB). Since almost 90 % of rifampicin resistant isolate are also resistant to isoniazid, mutation in rpoB gene become important as a surrogate marker for MDR-TB. MDR- TB isolates used for this research, namely isolate 885, was collected by Regional Health Laboratory of Surabaya. PCR was used to amplify the gene, on described steps : a cycle of preheating at 95°C for 15 minutes, amplifying in 45 cycles ( 1 minute at 94°C, 1 minute at 58°C, 1 minute 72°C) and post extension for 5 minutes at 72°C. The mutations were detected by sequencing and alignment using MEGA4. The result of this research showed that there were new mutations downstream of the core region of rpoB. Sequence analysis showed some mutations such as S594A, S626V, T629A. In conclusion, it is reported for the first time, new mutations at downstream region of the core region of rpoB.

Y-Chromosome Microsatellite DNA Variations on Terunyan Bali Mula Society I KETUT JUNITHA; SANG KETUT SUDIRGA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

The Balinese is comprised of genealogical patrilineal clans named as soroh. However, remnants of ancient Bali Mula communities have their own local genealogy which differ from the general soroh. The Pasek Trunyan together with Pasek Kayu Selem, Pasek Celagi, and Pasek Kayuan are part of Bali Mula which believed to be descendants of one ancestor, Empu Kamareka. Here we reported genetic variation of the Terunyan society. The microsatellites Y-chromosome markers (DYS19, DYS390, DYS393, and DYS395) were used in this study. There were 12 alleles found and the result showed the low genetic diversity (D = 0.28 + 0.05) within Terunyan society. Nine haplotypes based on allelic combination were found, dominated by major haplotype with frequency of 0.50 which spread out in many genealogical lineage called dadia. The haplotype data showed that Terunyan society derived from many gene sources. Key words: patrilineal, pasek, soroh, dadia, haplotipe

RAGAM ALEL DNA MITOKONDRIA MASYARAKAT SOROH PANDE DI BALI DENGAN METODE PCR-RFLP Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p12

Masyarakat Bali mengelompokkan diri berdasarkan soroh atau garis keturunan tetapi bukti genetik pengelompokkan tersebut belum diketahui. Salah satu soroh/ klan yang memiliki persebaran yang luas di seluruh Bali adalah soroh/ klan Pande. Penelitian ini dilakukan untuk mengetahui ragam alel DNA mitokondria masyarakat soroh Pande di Bali dengan metode Polymerase Chain Reaction-Fragment Length Polymorphism (PCR-RFLP) yang berguna untuk kepentingan forensik. Sampel sel epitel mukosa mulut diambil dengan cara purposive sampling yang terdiri dari 35 sampel warga Pande. Tahapan penelitian meliputi ekstraksi DNA dengan metode fenol-kloroform yang telah dimodifikasi, amplifikasi DNA pada daerah D-loop DNA mitokondria (mtDNA), digesti produk PCR dengan lima macam enzim restriksi dan elektroforesis dengan menggunakan gel agarose 1 % dan PAGE 10%. Penelitian ini mendapatkan sembilan ragam alel. Digesti dengan enzim Hae III, Hinf I, Mbo I dan Dde I mendapatkan masing-masing dua tipe pemotongan sedangkan enzim Hind III tidak memiliki situs pengenalan pada daerah D-loop DNA mitokondria. Enzim Hind III tidak dapat digunakan untuk membedakan profil DNA antar individu karena bersifat monomorfik.

Perubahan Golongan Darah Dan Identifikasi Bakteri Yang Berperan Dalam Merubah Golongan Darah Bagas Susilo; I Ketut Junitha; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p13

Blood is one of the most important component of human body. In a case of crime, blood found at the site can be used as an evidence to track back the victim or the person who did such criminal. The main objective of this research were to investigate the time needed for the convertion of blood types and to identify bacterial species with capability to convert blood types. This research was conducted at Forensic Laboratory and Microbiology Laboratory, Faculty of Mathematics and Science, Udayana University. The methods used for detection of blood types and identification of Bacterias were elution method and vitex method, respectively. The results showed that blood type A on iron surface and ceramic media was changed into fake O type on day 25, while blood types of B and AB on ironic media were converted into fake type of O after 30 days of exposure. Two species bacteria (Rhizobium radiobacter and Serratia ficaria) with ability to convert blood type were identified in this research. Key Word: Rhizobium radiobacter, Serratia ficaria, changes of blood types

Perubahan Golongan Darah Berdasarkan Pengaruh Waktu dan Mikroorganisme Yang Berperan Muhamad Masyrur; I Ketut Junitha; Meitini W. Proborini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p05

Blood is one of the most important evidence in the forensic analysis. Blood has a special characteristic or identity of the owner. Blood can be analyzed blood type to know the owner of the blood. But in the course of time, the blood will be degraded so it will be change of blood type into blood type O. One of the contributing factors is the presence of microorganisms that it grow in the blood. The material of this research is the human blood of blood type A, B, AB and O from 4 (four) respondents of 3 (three) males and 1 (one) female who are 20 to 40 years old. Each sampel of 150 µL was dropped on sterile gauze size 2 x 6 cm, then it was dried. Sample were stored at room temperature (± 30 oC) for 0, 30, 60, 90 and 120 days. Sampel was tested for blood type using absorption elution method based on the work instructions from The Police Forensic Laboratory of Denpasar Branch in 2016. The results of this research indicate that the human dried blood stored on sterile gauze media for 30 and 60 days has not changed its blood type. Storage of the human dried blood for 90 and 120 days, it have changed blood type with found antigen A or B that was not detected back through absorbtion elution analysis on blood group A, B and AB. This research found 9 species of fungi and 7 species of bacteria that they grow on the blood samples. Species of Fungi and bacteria that have high ability to degrading all blood group (A, B and AB) are Acromonium carticola, Alternaria alternata, Aspergillus flavus, Aspergillus niger, Penicillium sp and Bacillus coagulans. While species of fungi and bacteria that have high ability to degrading blood group B or AB are Fusarium sp, Trichoderma harzianum, Trichoderma viridae, Bacillus sp and Staphylococcus cohnii.

STRUKTUR GENETIK DAN RAGAM ALEL TIGA GENERASI MASYARAKAT BALI BERDASAR LIMA LOKUS PENANDA DNA MIKROSATELIT AUTOSOM Ayu Saka Laksmita; I Ketut Junitha; Ni Luh Watiniasih
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2015.v02.i02.p02

This research was aimed to find out the genetic structures of three generations of Balinese population, in order to determine the best loci used for paternity testing among this population, and observed the mutation rate of these loci. The DNA samples were taken from the epithelium cell of 25 families which were collected from the children, father, mother, grandfather and grandmother of the children, from both mother and father sides (family with three generations). The DNA was extracted in Phenol-Chloroform method with modifications. DNA amplification was conducted in PCR method using pairs of primer 5, namely: FGA, D18S51, D2S1338, TPOX, and D16S539, and its products were electrophoresed and visualized in 10% of PAGE, stained in silver nitrate. The genetic structures of the three family generations showed 30 variants with different frequencies in each locus. The highest heterozygosity value was detected in FGA (8 alleles), then followed by D18S51 (7 alleles), TPOX (6 alleles), D16S539 (5 alleles), and the lowest was in D2S1338 (4 alleles). The highest value of heterozigosity and Power of Discrimination were found in FGA, followed by TPOX, D18S51, D2S1338, and the lowest was in D16S539. Therefore, it can be concluded that out of five loci tested, 4 of them can be recommended to be used for paternity testing of Balinese population, except D16S539. Keywords: Paternity Testing, DNA Microsatellite, three generations, heterozygosity, Power of Discrimination

PENGARUH MUTAGEN KIMIA SODIUM AZIDA TERHADAP MORFOLOGI TANAMAN CABAI BESAR (Capsicum annuum L.) Ni Kadek Yunita Sari; Made Pharmawati; I Ketut Junitha
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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The aim of this research was to examine the effects of sodium azide at concentration of 1 mM, 2 mM, 3 mM, 4 mM and 5 mM on the morphological characters of Capsicum annuum L. The method used for the observation of morphological characters was direct observation by measuring plant height, leaf length and width as well as counting the number of leaves. The results showed that effect of sodium azide at 3 mM caused an increase in plant height, while concentration of 1 mM, 2 mM, 4 mM dan 5 mM caused a decrease in plant height. Similar trends were found for number of leaf. Leaf length and width tended to increase in sodium azide treatments.

METODE ANALISIS KUALITATIF DAN KUANTITATIF LDL-C MENGGUNAKAN ELEKTROFORESISAGAROSE DAPAR TAE (TRIS-ASAM ASETAT-EDTA) Putu Rosi Setyari; IMA Gelgel Wirasutha; I Ketut Junitha
Jurnal Farmasi Udayana Vol. 1, No. 1, Tahun 2012
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.269 KB)

In this research had been done the development of analysis qualitative and quantitative methods of LDL-C in human serum using agarose electrophoresis. The methods that had been developments are separation lipoprotein in human serum using agarose electrophoresis with TAE pH 8,6 buffer system and quantification methods for LDL-C using spectrophotometer UV-Vis. The aim of this research is to find out a simple method that can separate lipoproteins of human serum and quantificating LDL-C. The results of this research shows agarose electrophoresis with TAE pH 8,6 buffer system could separate LDL-C in human serum as good as electrophoresis with Na-Barbital buffer system. However, for its quantification only can be done with spectrophotometer UV-Vis.

RASIO JENIS KELAMIN PADA KELAHIRAN ANTARA BULAN DESEMBER 2010 SAMPAI NOVEMBER 2012 DI RSUD WANGAYA, DENPASAR DAN RSUD BANGLI, BANGLI DI PROVINSI BALI Victor Peter Poli; I Ketut Junitha; Ni Made Rai Suarni
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1056.698 KB)

Penelitian tentang rasio jenis kelamin pada kelahiran dilakukan untuk memperoleh informasi tentang rasio jenis kelamin pada kelahiran di Indonesia khususnya di pulau Bali. Penelitian ini juga bertujuan untuk mengetahui pengaruh faktor suhu lingkungan terhadap konsepsi yang berdampak pada rasio jenis kelamin. Data kelahiran selama dua tahun diperoleh dari RSUD Wangaya Denpasar dan RSUD Bangli sejak bulan Desember 2010 hingga November 2012. Data suhu dan curah hujan didapat dari Badan Meteorologi, Klimatologi, dan Geofisika (BMKG) wilayah III di Denpasar. Data yang diperoleh diuji dengan chi-square test dan regresi linier. Rasio jenis kelamin pada kelahiran di RSUD Wangaya yaitu 108 kelahiran laki-laki per 100 kelahiran bayi perempuan dan di RSUDBangli yaitu 94 kelahiran bayi laki-laki per 100 kelahiran bayi perempuan. Hasil analisis menunjukkan bahwa tidak ada hubungan antara konsepsi penentuan jenis kelamin dengan suhu. Dapat disimpulkan rasio jenis kelamin di RSUD Wangaya dan RSUD Bangli kemungkinan hanya dipengaruhi oleh peluang.

Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Desak Nyoman Budiningsih Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri Ni Putu Putri Wulandari Ni Putu Senshi Septiasari Putri Arie Prasetyoningrum Putu Rosi Setyari SANG KETUT SUDIRGA Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti

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