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All Journal METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Jurnal Veteriner
Ni Luh Putu Agustini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Monitoring Proviral DNA Of Jembrana Disease Virus In Bali Cattle Using PCR Ni Putu Eka Krisnayanti; Made Pharmawati; Inna Narayani; Ni Luh Putu Agustini
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p03

Abstract

The development of biotechnology in molecular field make it possible to detect the presence of a disease earlier, faster and more accurately. One of the technique in biotechnology which has been highly developed and often be used in biological and medical research is PCR method. This molecular technique allows to accurately identify the DNA level in presence of a particular disease. This study aims to do monitoring the presence of proviral DNA Jembrana Disease Virus in bali cattle from all regencies in Bali. The blood samples of bali cattle is taken from all regencies in Bali in 2017 as many 170 samples and in 2018 from Buleleng Regency there are 101 samples and form Karangasem Regency 152 samples. DNA is extracted by QIAamp DNA blood mini kit and samples from 2017 is made to 34 pool samples, whereas samples from 2018 is made to 20 pool samples (Buleleng Regency) and 30 pool samples (Karangasem Regency). PCR is conducted by JDV 1 primer and JDV 3 primer in total reaction 25 µL with 35 cycles. PCR is also conducted on positive control and negative control. The result indicate that 34 pool samples of bali cattle from 2017 or 20 pool samples and 30 pool samples from 2018 is not indicate the presence of proviral DNA Jembrana Disease Virus. Keyword: PCR, proviral DNA, Jembrana Disease Virus
Aktivitas Biologis Imunoglobulin Yolk Anti Parvovirus Setelah Perlakuan Suhu I Gusti Ayu Agung Suartini; Ni Luh Putu Agustini; Ni Luh Eka Setiasih; Sisyawati Putriningsih; Defi Lega Nurwidana
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.746 KB)

Abstract

This study aims to determine the effect of temperature on the biological activity of various crude and precipitate specific Immunoglobulin (Ig)Y Canine parvovirus (CPV). Hiperimun serum conducted on Isa Brown chickens injected with antigen CPV. Crude yolk Ig preparations derived from chicken serum without purification while the yolk Ig preparations precipitates obtained by the chicken serum was precipitated with ammonium sulfate and dialyzed. Both types of Ig yolk given treatment temperature 50ºC, 60ºC, 70ºC, and 80ºC for 15 minutes. To test Gel Precipitation Test (AGPT) is performed to determine whether there is a specific IgY CPV in the serum of chickens. Biological activity of both types of Ig detected with Barriers Haemagglutination test (HI). The design used in this study is completely randomized design factorial. The results of this study indicate that the temperature was highly significant on the activities of IgY crude and precipitates. Activities IgY crude and precipitate down to the treatment temperature of 50ºC, 60ºC, 70ºC, and 80ºC. Geometric Mean Titer crude IgY respectively - were 26.67, 26, 25.33, and <2º Unit HI while IgY precipitates are respectively 26.33, 25.67, 24, and <2º Unit HI. Based on the results of this study concluded that the biological activity of crude IgY better than IgY precipitates after treatment of a wide range of temperatures.
Kinetika Immunoglobulin Kuning Telur Antiparvovirus Anjing Pada Anjing (KINETICS OF ANTICANINE PARVOVIRUS YOLK IMMUNOGLOBULIN IN DOGS) I Gusti Ayu Agung Suartini; Indrawati Sendow; Ni Luh Putu Agustini; Agik Suprayogi; I Wayan Teguh Wibawan; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.941 KB)

Abstract

Kinetic study on Anti CPV IgY has been performed on six dogs aged 5-10 months. The IgY was injectedintravenously at dose of 21.4mg /10kg body weight. IgY levels in the blood were determined by ELISA. Aresearch was conducted to find out the kinetics of Anti CPV IgY in dogs blood. The kinetics of IgY wascalculated by using regression analysis to determine the association on the levels of IgY in serum againsttime at injection. The results showed that kinetic parameters were calculated based on first order kinetics.The constant elimination rate of IgY was at the range between 0.007 to 0.015 / h. IgY concentration in thedogs blood was from 0.746 to 0.992 mg / mL. The half-life of IgY was from 1.65 to 4.01 / d. Volumedistribution of IgY was between 21.47 to 28,55 / mL. Total IgY in the dog bodies (AUC) was from 42,60 to142,00 mg / mL.h. The duration of the IgY in the dog’s body was 3.08 to 8.51 days. Clearance time of IgY was0.15 to 0.50 mL / h. In conclusion the kinetics of anti CPV IgY in dog’s body follow one compartment andfirst order model, which are only distributed in the blood with the half-life at 2.5 days, and IgY has lesspossibility to accumulate in the body compared to the IgG.
Co-Authors Agik Suprayogi Defi Lega Nurwidana I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan Indrawati Sendow Inna Narayani MADE PHARMAWATI Ni Luh Eka Setiasih Ni Putu Eka Krisnayanti Sisyawati Putriningsih