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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Akademika Biologi
Endang Saptiningsih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Pertumbuhan Plantlet Anggrek Phalaenopsis amabilis (L.) Blume pada Tahap Subkultur dengan Perlakuan Jenis Media dan Konsentrasi Pepton Berbeda Anggi Krisdianto; Endang Saptiningsih; Yulita Nurchayati; Nintya Setiari
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p06

Abstract

Phalaenopsis amabilis is an orchid native of Indonesia that included in the appendix II category and one of the flowers included in the national and international flower trade, so that needs to be used in vitro propagation. The type of media used in the subculture determines the results of plantlet growth. Media used include New Phalaenopsis (NP), Vacint and Went (VW), and Murashige and Skoog (MS). Pepton as supplement added to the media. This study aims to analyze the different media types and peptone concentrations during the subculture. The method used is a plantlet subculture to a media bottle. This study uses a completely randomized design with 4x3 factorial patterns and 3 replications. Data were analyzed with ANOVA at 95% significance. Plantlet P. amabilis was subcultured in MS, VW and NP media with peptone (0; 1; 2; 3 g/L). Growth was observed for 2 months. The parameters observed were the number, length, width, appearance of leaves; number, length, appearance of the root. ANOVA test showed that the two treatments did not have interactions with all parameters, except leaf length. The results showed that the treatment of media type and peptone concentration significantly affected all parameters. NP media and peptone concentration of 3 g/L are the best media and concentrations for the number of leaves (5.67; 5.89), leaf appearance (19.83; 20.22 days ); leaf width (0.42; 0.47 cm), leaf length (1.47 cm); root length (2.83; 2.86 cm), number of roots (2.33; 2.56), and root appearance (28; 27.22 days). The combination of NP media with pepton concentration of 3 g/L is the best combination for the growth of P. amabilis plantlets at the subculture stage.
UJI TOTAL GLUKOSA DAN AKTIVITAS ANTIOKSIDAN BEBERAPA PRODUK PANGAN FUNGSIONAL BERBAHAN DASAR UBI JALAR UNGU (Ipomoea batatas L.) var AYAMURASAKI Edysri Hermayudha P; Munifatul Izzati; Endang Saptiningsih
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.336 KB)

Abstract

Functional food is food with high nutrition and important to illness terapy. Functional food product based of purple sweet potato are an alternative to replace primary food. This research purpose to know the quantity of total glucose, antioxydant activity, and antosianin decreaseful colour in a few of functional food product based of purple sweet potato. This research take place in food technology laboratoryum Unika Semarang on Desember until January 2013. This research use RAL single factor of food making process include milk, ice cream, wet noodle, dry noodle, and flour look for quantity of total glucose, antioxidant activity, and antosianin decreaseful colour. Method of research include analyse quantity of total glucose assay fenol method, antioxidant activity assay Diphenyl Pycril Hydriazil (DPPH), and observation in antosianin decreaseful colour product. The result of research show that the best food product is purple sweet potato flour. That product has quantity of total glucose 2,805 g/100 g, IC50  - 49951 %, and antosianin colour product take no damage because it still look purple. Keyword: the functionality food product, total glucose quantity, antioxydant activity IC50, antosianin decreaseful colour
PENGARUH NAUNGAN TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN BAWANG DAUN (Allium fistulosum L.) DI BANDUNGAN, JAWA TENGAH Ismi Alfii Anni; Endang Saptiningsih; Sri Haryanti
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Onions ( Allium fistulosum L. ) is a vegetable that is often consumed by public . People often consume on the part of young leaves . Onions production decreased during the rainy season . One way to increase the growth and production of onions by adjusting the light intensity that is appropriate . Shade is one effort intensity of the sun setting to the plant . The purpose of this research was to determine the effect of shade on the growth and production of onions . The research conducted at Sidomukti Village, Bandungan and laboratory BSF, FSM, Diponegoro University. The research design using the single factor RAL: shade . Data analysis using ANOVA followed by Duncan's Multiple Range Test Test ( DMRT ) confidence level 95 % . The parameters were  plant height , number of tillers , wet weight , dry weight , chlorophyll content , stomatal number . The results showed that the difference was not significantly different shade to the number of tillers , fresh weight , chlorophyll content , and the number of stomata , but showed significantly different results to height and plant dry weight . Paranet shade with 2 coats of 10950-24850 lux light intensity and give the highest production growth in height and plant dry weight. Keyword: onions, growth, production
Co-Authors Anggi Krisdianto Edysri Hermayudha P Ismi Alfii Anni Munifatul Izzati Nintya Setiari Sri Haryanti Yulita Nurchayati