Claim Missing Document
Check
Articles

Found 39 Documents
Search

KANDUNGAN ASAM ASKORBAT PADA KULTUR KALUS ROSELA (hibiscus sabdariffa L.) DENGAN VARIASI KONSENTRASI SUKROSA DALAM MEDIA MS Nurchayati, Yulita; Afiah R, Fathiyah
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.858 KB) | DOI: 10.14499/mot-TradMedJ15iss2pp%p

Abstract

Asam askorbat merupakan salah satu antioksidan yang dijumpai dalam kelopak bunga rosela. Produksinya secara konvensional memerlukan waktu dn lahan yang luas, sehingga diperlukan metode alternatif menggunakan pembentukan kalus secara in vitro.  Sukrosa  berperan sebagai sumber karbon utama dalam medium MS, slain itu dapat berperan sebagai prazat pembentukan asam askorbat.  Penelitian ini bertujuan untuk mengkaji pengaruh penambahan sukrosa dalam pembentukan kalus dan produksi asam askorbat.  Perlakuan yang diberikan adalah beberapa konsentrasi sukrosa yaitu 20 g/L, 30 g/L, 40 g/L dan 50 g/L dalam medium kultur.  Kultur diinkubasi selama 42 hari dengan subkultur 2 kali setiap 10 hari sekali. Analisis kandungan asam askorbat dilakukan dengan metode titrasi iodometri. Hasil penelitian menunjukkan bahwa kalus terbentuk paling cepat pada penambahan sukrosa 20 g/L sedangkan kandungan asam askorbat tertinggi diperoleh pada kalus dengan penambahan sukrosa 50 g/ L.
Growth Pattern and Copper Accumulation in Callus of Datura metel Nurchayati, Yulita; Santosa, Santosa; Nugroho, Laurentius Hartanto; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5177

Abstract

This experiment was aimed to evaluate the copper accumulation using callus culture of Datura metel L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl2.2H2O and Na2CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 M. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20th doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl2.2H2O at 5 15 M of level concentration but declined at 20M. The maximum rate of accumulation of Cu (0,1519 mg g-1) was obtained at 15 M. Instead, the addition of Na2CuEDTA at 5 20M of level concentration showed the significant increment while the maximum accumulation was obtained at 20M (0,1420 mg g-1). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of D. metel as that fairly absorbed and accumulated Cu2+. Exposure with CuCl2.2H2O indicated higher accumulation than Na2CuEDTA.How to CiteNurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. Biosaintifika: Journal of Biology & Biology Education, 8(2), 135-140.
The Effect of NAA and Ga3on The Growth Performance of Ceriops tagal Seedling Budihastuti, Rini; Prihastanti, Erma; Nurchayati, Yulita
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13625

Abstract

Plant Growth Regulator (PGR) had been widely utilized in improving plant quality and productivity. The mechanism includes the manipulation of plant adaptibility over environmental stress. This research aimed to study the efect of PGR on the growth of C. tagal seedling and to formulate the applicable treatment to improve its growth performance. The research was conducted through laboratory experiment, including tthe combination of NAA and GA3 with the designed concentration of 0 ppm, 150 ppm and 300 ppm. The experiment lasted for one month and involved weekly observation. The plants were treated by spraying once every three days. Observations were conducted to the survival rate, height increment and leaf number development. ANOVA was conducted as statistical analysis method. The result showed that there were no significant effect of PGRs on the height increment of C. tagal seedling. The survival rate was lowest in the control and 150 ppm NAA treatments, while treatment with 300 ppm GA3was highest. The application of 300 ppm GA3was the best treatment for the livelihood of C. tagal seedling, while the application of 150 ppm GA3 was considerable alternative due to its growth performance. This research provide a novel finding concerning the application of PGRs on the seeding process of C. tagal. Thus, it could be applied in the seeding activities by the mangrove farmers to improve the productivity and quality of mangrove seedling.
Induksi Kalus dari Hipokotil Alfalfa (medicago sativa l.) secara in vitro dengan Penambahan Benzyl Amino Purine (BAP) dan α-Naphtalene Acetic Acid (NAA) Hayati, Surya Kurnia; Nurchayati, Yulita -; Setiari, Nintya -
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 1, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.893 KB) | DOI: 10.14710/bioma.12.1.6-12

Abstract

Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, andhepatitis. Propagation of this plant in Indonesia face a problem which has no embryo. One method to propagate thisplant is by tissue culture or micropropagation. Callus induction is first step in micropropagation to produce calluswhich will be regenerated to become planlet. The aims of this research are to induce callogenesis from hipocotyl ofalfalfa with addition Benzyl Amino Purine (BAP) and α Naphtalene Acetic Acid (NAA), and to determine the propercombination of BAP and NAA to produce the optimal callus. The experiment has been conducted by using 12combination of BAP and NAA with Completely Randomized Design (CRD) in 4x3 factorial pattern by 5 replicates.Data were analyzed by ANOVA 95% Degrees of Freedom (DF). If there was significance result, it was followed byDMRT analyzed at 95 % DF. The result showed that combination of BAP and NAA was able to induce callogenesisfrom hipocotyl of alfalfa. The optimal callus was obtained in combination of BAP 0 ppm and NAA 2 ppm.
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Rosela (Hibiscus sabdariffa L.) dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
Bioma : Berkala Ilmiah Biologi Vol. 14, No. 2, Tahun 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.682 KB) | DOI: 10.14710/bioma.14.2.85-90

Abstract

Callus induction method can be used as tool in producing plant secondary metabolites. One of this compound found in roselle (Hibiscus sabdariffa L.) is antioxidant agent i.e ascorbic acid and carotenoid. The callus could be induced from any kind of explants in tissue culture. The aims of this experiment is to select explant which can encourage of callus formation beside high level of antioxidant compounds. The treatment of experiment was three kinds of explants i.e section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L NAA dan 5 mg/L BAP.  This experiment has been conducted by completly randomized design with 5 replicates Growth variable, included fresh weight callus, growth presentation, callus respons and biochemical variable like content of ascorbic acid and carotenoid were analyzed.  The data were analyzed by analysis of varian and Duncan’s test at 95% significant level. Thed results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds.   Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
Eksplorasi Kandungan Klorofil pada beberapa Sayuran Hijau sebagai Alternatif Bahan Dasar Makanan Tambahan Setiari, Nintya -; Nurchayati, Yulita -
Bioma : Berkala Ilmiah Biologi Vol. 11, No. 1, Tahun 2009
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (44.214 KB) | DOI: 10.14710/bioma.11.1.6-10

Abstract

Chlorophyll is a plant pigment which recently have been popular as food supplement. The source ofchlorophyll consumed now are alfalfa’s leaves algae such as Spirulina and Chlorella.. Chlorophyll content find in allkinds of plant especially vegetables so that have potent to be source of chlorophyll. The aims of this research are toexplore alternative source of chlorophyll aside of Leguminosae, from vegetables such as sweet basil, spinach, waterspinach, cassava leaves, pegagan, and papaya’s leaves and to study the vegetables potent to be food supplement.The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85%. The extract was filteredwith Whatman paper No. 1, thus record the absorbance by using spektrofotometer UV Vis. Statistical analysis wasused to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in95% significancy. If the ANOVA showed significantly different, the analysis were continued with Duncan’s test.The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves. However thelowest one was found in sweet basil. Papaya’s leave potential as food supplement.
Pengaruh Perendaman Akar Bibit Bayam Merah (Alternanthera amoena Voss.) dalam Larutan Na2CuEDTA terhadap Pertumbuhan dan Kandungan Antosianin Aini, Nabilah; Nurchayati, Yulita; Suedy, Sri Widodo Agung
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.173 KB) | DOI: 10.14710/bioma.20.2.123-132

Abstract

Red spinach is one of plants containing anthocyanin pigment which has various functions as food and beverage dye, and play a role in the field of health. Anthocyanin biosynthesis in plants is influenced by environmental factors, one of them are nutrients. One of the nutrients needed by plants and part of the anthocyanin biosynthesis pathway is copper (Cu). Cu can be added in the form of a fertilizer such as Na2CuEDTA. This research aims to study the effect of soaking the red spinach in Na2CuEDTA solution on growth and anthocyanin content, and knowing the optimal Na2CuEDTA concentration for growth and anthocyanin content of red spinach. This research conduct to Complete Random Design of single factor pattern with 5 treatments and 5 replications which are aquades, nutrient solution, nutrient solution+Na2CuEDTA 5 ppm, nutrient solution+Na2CuEDTA 10 ppm, nutrient solution+Na2CuEDTA 20 ppm. Sprouts red spinach that already has 4 leaves soaked in the treatment solution for 10 days, then the plants moved into a soil medium inside the pot for 20 days. The analyzing data which is used is ANOVA continuing with further testing of Duncan Multiple Range Test (DMRT) at the 95% significance level. The result of this research show that soaking of red spinach in Na2CuEDTA solution did not show any significant effect on growth. The growth of red spinach plant is more influenced by nutrient solution while the anthocyanin contents is influenced by nutrients in soil medium. Key words : Red Spinach, Copper, Anthocyanin, Na2CuEDTA
Produksi dan Profil Metabolit Bunga Krisan (Chrysanthemum sp.) pada Intensitas Cahaya Lampu LED dengan Durasi Yang Berbeda Utami, Ika Nur; Nurchayati, Yulita; Hastuti, Endah Dwi
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.797 KB) | DOI: 10.14710/bioma.21.2.154-164

Abstract

Chrysanthemum is a Short Day Plant (SDP) that will flower if the day length is less than 12 hours. Indonesian Chrysanthemum farmers provide additional light at night to maintain the Chrysanthemum’s vegetative phase so obtained the stem length which accordance to the cut flower standard (±76 cm). In other side, Chrysanthemum flowers are known has contain metabolites product and  potentially useful. This study is purpose to determine the effect of differences in LED light intensity and light exposure duration on growth, flower production, and metabolites profile of chrysanthemum. The study used a RAL method 3x2 factorial pattern which is in the form of giving an additional light intensity of 0 W, 10 W and 20 W and an additional light exposure duration of 2 hrs and 4 hrs. The results showed that the combination of 20 W + 4 hrs is optimaly increases the stem length (96,2 cm) and flowers diameter (6,6 cm). The 20 W light intensity is optimally inhibits the flower initiation and increases the amount of flower. The most compounds produced by chrysanthemum are from fatty acid groups, then hydrocarbons, and diterpenes. The combination of 10 W + 4 hrs is an optimally increases flower metabolites production that produces the most compounds compared to other treatments.
Produksi Biomassa Tanaman Nilam (Pogostemon cablin) yang Ditanam pada Intensitas Cahaya yang Berbeda Darmanti, Sri; Nurchayati, Yulita; Hastuti, Endah Dwi; Syaifuddin, Mochammad
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.667 KB) | DOI: 10.14710/baf.v17i1.2532

Abstract

Patchouli oil is found in all vegetative part of Pogostemon cablin. Therefore, a high biomass is important to increase production of patchouli oil. Biomass production is on of plant growth indicator. Plant growth is a effected by genetic and environmental factor. Light intensity will effect to chlorophyl syntesis, photosynthetic rate, transpiration and hormonal balance. The aim of this research is to study effect of light intensity on biomass production of Pogostemon cablin. Design of research is CRD (Complete Randomized Design), with one factor which is light intensity. There were difference of light intensity that use astreatments. These are : 96 LUX, 340 LUX and 780 LUX. Each treatment was replicated five times. Result indicated that, light intensity affect biomass production of Pogostemon cablin. At light intensity of 96 LUX, biomass productions is the most optimum. An increase of light intensity, biomass production ofPogostemon cablin is reduction.
PENGARUH ALELOKIMIA EKSTRAK TANAMAN PADI (Oryza sativa L. Var. IR64) TERHADAP PERKECAMBAHAN DAN PERKEMBANGAN KECAMBAH KEDELAI (Glycine max L.) Mahayaning, Fitrian Agna; Darmanti, Sri; Nurchayati, Yulita
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.109 KB) | DOI: 10.14710/baf.v23i2.10021

Abstract

The aim of this research is to know the allelochemicals effect of  rice var. IR 64 residues extract on germination and growth soybean var. Grobogan seedling. This experiment  uses completely randomized factorial design (3x5). The fist factors is extract resources (root, straw, and hull) and secont factor is level concentration extrac (0%, 25%, 50%, 75%, dan 100%) with 5 replicates. Data analysed using ANOVA and DMRT.The results showed that allelochemicals from residue of rice IR 64 extract inhibit the germination and growth soybean var. Grobogan seedling, the higher concentration of the treatment the greater inhibition. Inhibition most likely caused by a hull rice extract.   Keywords: Glycine max, Oryza sativa, allelochemical, phenol