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Didik Handijatno
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, Kampus C Unair, Jalan Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia, 60115
Veterinary

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Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.
Analisis Filogenetik Gen Hemaglutinin dan Neuraminidase Avian Influenza H9N2 Asal Ayam Petelur di Jawa Timur (PHYLOGENETIC ANALYSIS OF HAEMMAGLUTININ AND NEURAMINIDASE GENES OF AVIAN INFLUENZA H9N2 FROM LAYER INI EAST JAVA) Prestalia Dwi Rachmawati; Tatang Santanu Adikara; Hani Plumeriastuti; Rahaju Ernawati; Jola Rahmahani; Didik Handijatno; Christian Marco Hadi Nugroho
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza virus (AIV) subtype H9N2 is one of the infectious agents that threatens laying poultry farms, because it has an impact on drastically reducing production in the population. The aim of this study was to isolate and analyze phylogenetically the partial gene encoding the surface proteins of the AIV subtype H9N2 from laying hens in East Java. A total of 30 suspected AIV subtypes of H9N2 were taken from laying hens which had decreased production by up to 70% in three sub districts each in Kediri, Blitar and Tulung Agung regency, in East Java Province. The virus was isolated in embryonated chicken eggs and then followed by a Hemagglutination (HA) test. Detection of the presence of H9 and N2 genes was carried out through Reverse Transcriptase polymerase Chain Reaction (RT-PCR) and continued with partial gene sequencing of the two surface proteins. Data analysis was processed using BioEdit version 7.2.5 and Mega 7.0. The results show that only one sample, namely code B2.2 from Blitar regency, is an AIV subtype of H9N2. The virus in this study belong to clade h9.4.2.5 of the AIV subtype H9N2. The conclusion of this study is that the VAI subtype H9N2 was successfully isolated from laying hens in East Java and successfully identified phylogenetically.
Co-Authors Christian Marco Hadi Nugroho Endang Suprihati Fedik Abdul Rantam Hani Plumeriastuti Lucia Tri Suwanti, Lucia Tri Mufasirin Nunuk Dyah Retno Lastuti Prestalia Dwi Rachmawati Rahaju Ernawati Rahmahani, Jola Romy Muhammad Dary Mufa Tatang Santanu Adikara