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Elevation of Bcl2 expression in artery spiralis of pregnant Rattus norvegicus infected with Tachyzoite of Toxoplasma gondii with hyperbaric oxygen therapy Arif Rahman Nurdianto; Aryati Aryati; Mohammad Guritno Suryokusumo; Mufasirin Mufasirin
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 3, No 2 (2019)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v3i2.2706

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ABSTRACTSpiral artery apoptosis plays a role in the process of abortion. Low Bcl2 expression found in abortion and in spiral artery. In pregnancy, Toxoplasmosis infection is found to increase apoptosis in spiral arteries. Hyperbaric Oxygen Therapy (HBOT) improves the expression of Bcl-2. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of Bcl2 in artery spiralis of pregnant rats infected with tachyzoite of Toxoplasma gondii. This is an experiment with a ‘randomized control group of post-test only design’ on 37 Rattus novergicus Sprague Dawley rats. Rats were divided into four groups. The group A is pregnant rats infected with 103 tachyzoites via intraperitoneal injection and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is pregnant only and received HBOT. Group C is pregnant and infected with tachyzoite but did not received HBOT. And the last, Group D is pregnant rats with no infection and did not received HBOT. Examinations of Bcl2 expressions were performed on day-5 after HBOT (twice a day). The Bcl2 expression was measured with immunohistochemistry. All data were tested with One-way ANOVA from SPSS 21.  There is an increased expression of Bcl2 spiralis artery in the Group A. There was a significant difference between Group A and Group C with a value of p=0.042. HBOT can increase the expression of Bcl2 from the spiral arteries of rats, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 days.Keywords: Toxoplasma gondii, tachyzoite, Hyperbaric Oxygen Therapy, Bcl2, spiralis arteryCorrespondence to: didins99@gmail.com  ABSTRAK Apoptosis arteri spiralis berperan dalam proses aborsi. Ekspresi Bcl2 rendah ditemukan pada aborsi dan di arteri spiral. Pada kehamilan, infeksi Toxoplasmosis ditemukan meningkatkan apoptosis pada arteri spiralis. Terapi Oksigen Hiperbarik (HBOT) meningkatkan ekspresi Bcl-2. Ini adalah penelitian eksperimental dengan desain post-test only pada 37 hamil Rattus novergicus Sprague Dawley, maka tikus dibagi menjadi 4 kelompok. Grup A HBOT adalah tikus hamil yang terinfeksi tachyzoite yang menerima terapi 10 sesi HBOT 2.4 ATA dalam 3x30 menit; kelompok B adalah Hamil saja dan tidak mendapat HBOT; kelompok C Hamil dan terinfeksi tachyzoite T.gondii tetapi tidak menerima HBOT; dan kelompok D adalah tikus hamil normal. Setiap tikus hamil yang terinfeksi diberi 103 Tachyzoite Toxoplasma gondii melalui intraperitoneal. Pemeriksaan ekspresi Bcl2 dilakukan pada hari ke-5 setelah HBOT (dua kali sehari). Tikus terbunuh dan kadar Bcl2 Arteri spiralis diukur dengan pemeriksaan Immunohistokimia. Semua data dianalisis dengan uji ANOVA dengan Program SPSS 21. Pada studi ini menunjukkan terapi Oksigen Hiperbarik dapat meningkatkan Ekspresi Bcl2 Arteri Spiralis dengan dosis 2.4 ATA selama 3x30 menit dalam 10 sesi selama 5 hari terapi. Studi ini menyimpulkan bahwa HBOT dapat meningkatkan ekspresi Bcl2 dalam arteri spiralis, dalam pemberian HBO 2.4 ATA selama 3x30 menit dalam 10 sesi.Kata kunci: HBOT, Arteri spiralis, Bcl2, Tachyzoite Toxoplasma gondiiKorespondensi: didins99@gmail.com 
THE EFFECTS OF CONCENTRATE FEED AND MINERAL BLOCK ON THE DENSITY LEVEL AND MILK FAT CONTENT OF DAIRY CATTLE Romziah Sidik; Desty Shafira; Mufasirin Mufasirin
Indonesian Journal of Applied Sciences Vol 7, No 2 (2017)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.207 KB) | DOI: 10.24198/ijas.v7i2.14383

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AbstractThe aim of this research is to understand the influence of concentrate feed and mineral block formulation on the densitylevel and milk fat of dairy cattle. The research was conducted on eight cross breed friesian holstein cows with ± 450 kg body weight and aged 2-4 years with milk production 8-11 liter/head/day. The experimental design of this study was complete randomized design with two treatments and each treatment was repeated four times. P0 (grass, tofu waste) and P1 (grass, tofu waste, concentrate feed, mineral block). The data was analyzed using t test (independent sample t test ). The software that was used to analyzed the data is Statistical Program for Social Science (SPSS) version 20  for Windows. The results showed that concentrate feed and mineral block did not significantly affect the density of milk and milk fat (p > 0.05). The mean density of milk in the P0 was 1.0255 and P1 was 1.0253. Mean while the average milk fat in the P0 was 3.27% and in P1 was 3.45%. Keywords : concentrate, mineral block, density, fat, dairy cow
Prevalensi Parasit Saluran Pencernaan pada Kerbau (Bubalus bubalis) Melalui Pemeriksaan Feses di Kabupaten Agam Sumatra Barat Muhammad Ridwan; Lucia Tri Suwanti; Tri Wahyu Suprayogi; Mufasirin Mufasirin; Kusnoto Kusnoto; Poedji Hastutiek
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.607 KB) | DOI: 10.33772/jitro.v9i1.19763

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ABSTRAKPenelitian ini bertujuan untuk mengetahui jenis, prevalensi, dan pengaruh jenis kelamin serta umur terhadap prevalensi parasit saluran pencernaan pada kerbau di Kabupaten Agam Provinsi Sumatra Barat. Sampel yang digunakan dalam penelitian ini adalah feses dari 105 ekor kerbau yang dikumpulkan dari tiga Kecamatan. Pemeriksaan sampel dilakukan dengan menggunakan metode natif, sedimentasi, dan pengapungan. Penelitian ini memberikan hasil bahwa ditemukan infeksi saluran pencernaan pada kerbau berupa cacing Paramphistomum sp. 31,43%, Fasciola sp. 23,81%, Oesophagustomum sp. 1,9%, Bunostomum sp. 0,95%, Strongyloides sp. 0,95%, Trichostrongylus sp. 0,95%, dan protozoa Eimeria sp. 2,86%. Prevalensi yang diperoleh sebesar 45,7% (48/105). Hasil analisis statistik menunjukkan bahwa jenis kelamin dan umur tidak mempengaruhi prevalensi parasit saluran pencernaan pada kerbau di Kabupaten Agam Sumatra Barat.Keywords: cacing dan protozoa, kerbau, prevalensi, saluran pencernaanPrevalence of Gastrointestinal Parasite in Buffalo (Bubalus bubalis) Through Feces Examination in Agam Regency West SumatraABSTRACTThis study aims to determine the type, prevalence, and influence of sex and age on the prevalence of gastrointestinal parasites on buffalo in the Agam Regency of West Sumatra Province. The samples used in this study were feces from 105 buffaloes collected from three Districts. Examination of the sample is carried out using by direct smear methods, sedimentation, and flotation. This study provides results that found gastrointestinal infections in buffalo by helminth Paramphistomum sp. 31.43%, Fasciola sp. 23.81%, Oesophagustomum sp. 1.9%, Bunostomum sp. 0.95%, Strongyloides sp. 0.95%, Trichostrongylus sp. 0.95%, and protozoa Eimeria sp. 2,86%. Prevalence is 45.7% (48/105). The results of statistical analysis showed that sex and age did not affect the prevalence of gastrointestinal parasites on buffalo in the Agam Regency of West Sumatra.Keywords: buffalo, gastrointestinal, helmint and protozoa, prevalence
THE EFFECT OF INCUBATION TIME ON EXCYSTATION OF EIMERIA TENELLA SPOROZOITES IN VITRO Joko Prastowo; Mufasirin Mufasirin; Sumartono Sumartono
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8596

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This purpose of the research is to know the effect of incubation time on excystation of Eimeria tenella sporo-zoites in vitro.Eimeria tenella oocystes treated in clorox were broken by glass beads. Sporocystes liberated from oocys were divid­ed in six groups (I - VI), each ghroup contained 1.68.106 sporocystes. All of the groups were treated by 0,75% taur-ocholat sodium and 0,25% trypsin. Those sporocystes were incubated at 41°C for 15, 30, 45, 60, 75 and 90 minutes respectively. The data collected was the percentage at the number of sporozoites found in each group.The result showed that the increase of the number of sporozoites was paralelly with the incubation time. The highest number of sporozoites excystation was found in the 90 minutes of incubation group.
Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.954 KB)

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Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.
Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.288 KB)

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This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.
Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya Fransiska Cicilia Beka; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Koesdarto Koesdarto; Mufasirin Mufasirin; Endang Suprihati; Dyah Ayu Kurniawati
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.657 KB) | DOI: 10.19087/jveteriner.2021.22.3.383

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Penelitian ini bertujuan untuk mendeteksi E. suis pada babi yang dipotong di Rumah PotongHewan (RPH) Pegirian, Surabaya. Sebanyak 63 sampel feses babi dikoleksi dari RPH Pegirian Surabaya. Deteksi morfologis Entamoeba spp. dilakukan dengan metode apung (analisis parasitologi). Selain itu, penentuan spesies E. suis dilakukan dengan PCR menggunakan primer Esuis764 (5’-ATC AAA TCA ATT AGG CAT AAC TA-3’) dan Esuis765 (5’-AAT TAA AAC CTT ACG GCT TTA AA-3’) dan prodak PCR yang diharapkan adalah 320 bp. DNA dari sampel positif diekstraksi dengan DNAzol. PCR dilakukan pada beberapa sampel positif yang dipilih secara acak dari analisis parasitologi. Hasil penelitian menunjukkan 57,14% (36/63) sampel secara morfologis positif Entamoeba sp. Hasil penentuan spesies berdasarkan analisis molekuler menemukan bahwa protozoa yang menginveksi babi di Rumah Potong Hewan Pegirian, Surabaya adalah E. suis.
Perbedaan Larva Stadium Kedua dan L2 Toxocara canis pada Jaringan Mencit Menggunakan Scanning Electron Microscopy (THE DIFFERENCES BETWEEN SECOND STAGE LARVAE AND L2 TOXOCARA CANIS ON MICE TISSUE BY USING SCANNING ELECTRON MICROSCOPY) Vindo Rossy Pertiwi; Kusnoto Kusnoto; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Mufasirin Mufasirin
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.144 KB) | DOI: 10.19087/jveteriner.2019.20.3.390

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Toxocariasis is one of zoonosis diseases that caused by Toxocara spp. that is Toxocara canis. Toxocara canis has several stages until it can infect animals and humans, namely the egg stage, larvae first stage (L1), larvae second stage (L2), larvae third stage (L3) to adult worms. Studies about the L2 and L2 tissue of T. canis found in paratenic hosts using Scanning Electron Microscopy (SEM) have not been widely performed. Some of the causes include L2 being not easily to found and identified, so research rarely raises the ultrastructural morphology of L2 and L2 tissues. Knowledge about the ultrastructural morphology of L2 and L2 tissue of T. canis worms is very important to determining the diagnosis, especially the etiological diagnosis. The purpose of this study is to detected morphology of L2 and L2 tissues of T. canis using SEM. Samples from this study is faeces of dogs that infected with toxocariasis and the digestive tract of dogs obtained from dog slaughter houses. The sample is an adult worm of T. canis; the female worm is dissected and taken uterus to collect worm eggs. The results of this study on microscopic and optilab examination showed a difference between L2 and L2 tissue that the length of L2 hatched from embryonic eggs was 390 ìm and with a width of 23.4 ìm at the midpoint of the body. Larvae second stage length from the infected somatic tissue is 410 ìm and the width is 22.5 ìm at the midpoint, and then difference in dorsal lip, cuticles, body ring, cervical alae, buccal capsul, tail.
Vaksininasi Protein Ekskretori-Sekretori Toxoplasma gondii Hasil Biakan in vivo Membangkitkan Respons Imun Non Protektif (THE VACCINATION OF Toxoplasma gondii EXCRETORY-EXCRETORY PROTEINS FROM IN VIVO CULTURE ENHANCED IMMUNE RESPONSE UNABLE PROTECTIVE) Mufasirin .; Endang Suprihati
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.743 KB)

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The aims of this research was to explore the immune response and protectiveness of mice whichwere vaccinated with Toxoplasma gondii excretory-secretory proteins produced from in vivo culture. A totalof 50  Balb/c strain mice were allotted  into five groups. Mice in group 1 to 3 were vaccinated with 20.7 kDa,35.3 kDa, and 100.9 kDa of the protein, respectively.  Whereas mice in group 4 were given total  excretory-secretory antigen ( total ESA5), and mice in group 5 were used as  control (PBS).  Booster  vaccinated wasconducted at two weeks following the first vaccination. Prior the challenge test, five mice were sacrificed forimmunologlobulin-G (IgG) analysis. The analysis of IgG using enzyme linked immunosorbent assay (ELISA).Challenge test used 1x103 T. gondii tachyzoites which were given to the animals   intraperitoneally.  Theresults showed that vaccination using  T.gondii excretory-secretory proteins as well as T. gondii total ESAscould enhanced immune response as detected by the markedly  presence of T. gondii  IgG.  However, thiswas unable to protect against T. gondii RH strain infection.
Zoonotic potential of gastrointestinal parasite in long-tailed Macaque Macaca fascicularis at Baluran National Park, Situbondo, East Java, Indonesia Dyah Ayu Kurniawati; Lucia Tri Suwanti; Nunuk D.R. Lastuti; Setiawan Kusdarto; Endang Suprihati; Mufasirin Mufasirin; Arif Pratiwi
Aceh Journal of Animal Science Vol 5, No 1 (2020): July 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.1.15397

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Baluran National Park (BNP) is one of the highest number of tourist visit among Indonesian national park. In the past decades, excessive feeding has induced change in macaque behaviour which increased the number of recorded human-macaque interaction. The close contact between macaque and humans can increase the risk of disease transmissions. This study aimed to identify gastro intestinal (GI) parasite in the long-tailed macaque. To provide identification, we adopted morphologic methods. We collected 100 faeces from unidentified individuals of Long-tailed macaque in BNP. Fecal samples were tested using direct smear and modified sugar floatation techniques. Microscopic examination showed 89% (89/100) samples were found to be positive of GI parasite. The prevalence of protozoa infection was higher (89%) than helminth (83%). The most prevalent GI parasite is Trichostrongylus sp (66%) following with  Entamoeba sp. (53%), Strongyloides sp. (32%), Blastocystis sp. (32%), Trichuris sp.(17%), Giardia sp. (10%) and Enterobius sp. (3%). All of GI parasite that successfully identified have zoonotic concern. In conclusion, GI parasites found in faeces of long-tailed macaque at Baluran National Park  potentially a zoonotic transmission.Keywords : GI Parasite; Long tailed Macaque; Baluran National Park; Zoonosis
Co-Authors Abdul Samik Adiana Mutamsari Witaningrum Adiana Mutamsari Witaningrum Agus Sunarso Alfina Azkiana Amalia Rosydinasari Rosydinasari andi jayawardhana Andi Jayawardhana Arif Pratiwi Arif Pratiwi Arif Rahman Nurdianto Aryati Aryati Azizah Bilqis Nurkarimah Benjamin Christoffel Tehupuring Boedi Setiawan Desty Shafira Dewi Purwatiningsih Dhimar Maulud Dyahningrum Didik Handijatno Djoko Legowo Doohan Mahendra Dyah Ayu Kurniawati Dyah Ayu Kurniawati Dyah Ayu Kurniawati Eduardus Bimo Aksono Herupradoto Elok Apriliawati Emy Koestanti Sabdoningrum Endah Rochmatika Endang Suprihati Endang Suprihati Erma Safitri Fauziah Fitri Hernanto Fedik Abdul Rantam Fransiska Cicilia Beka Gunanti Mahasri Hana Eliyani Hana Eliyani Hana Eliyani Hani Plumeriastuti Hanna Harnida Hartono Hartono Heni Puspitasari Heni Puspitasari Heni Puspitasari Heni Puspitasari Herry Agoes Hermadi Ira Sari Yudaniayanti Iwan Sahrial Hamid Joko Prastowo Khalissa Farah Alifia Koesdarto Koesdarto Kusnoto Kusnoto Lestari Wilujeng Lilik Maslachah Lucia Tri Suwanti Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Luthfiyyah Nur Afifah Siswandi Maslichah Mafruchati Melani Anggraini Melanie Aulia Ashfiyah Mochammad Amin Alamsjah Mohamad Yusuf Mohammad Guritno Suryokusumo Muchammad Yunus Muchammad Yunus Muhammad Ahdi Kurniawan Muhammad Ridwan Muhammad Ridwan Nedi Nedi Nenny Harijani Nenny Harijani Ni Komang Aprilina Widisuputri Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurdianto, Arif Rahman Nusdianto Triakoso Nusdianto Triakoso P Kumaladewi Paraswita Eindah Fitri Poedji Hastoetik Poedji Hastutiek Primarizky, Hardany R. Tatang Santanu Adikara Rahadju Ernawati Ramadhana Ramadhana Ratna Damayanti Rekasni Adallin A/P Morgan A/P Morgan Romy Muhammad Dary Mufa Romziah Sidik Sapto Andriyono Septian Hakim Susantoputro Sesa Puput Setiawan Koesdarto Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Chusniati Sumartono Sumartono Sunarso, Agus Sunaryo Hadi Warsito Talita Yuanda Reksa Taufan Ary Handoko Tri Wahyu Suprayogi Trifena Pristi Anindyta Tyasningsih, Wiwiek Vindo Rossy Pertiwi Wahidan Qodiip Maulana Warda Nafalizza Efendi Warsito, Sunaryo Hadi Win Darmanto Win Darmanto Wiwik Misaco Yuniarti Wurlina w wurlina wurlina Yulianna Puspitasari