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All Journal E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)
NI KADEK VENIARI
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana Jl. PB. Sudirman Denpasar 80362 Bali
Agriculture, Biological Sciences & Forestry

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Deteksi Cucumber Mosaic Virus (CMV) dan Chili Veinal Mottle Virus (ChiVMV) pada Gulma Commelina spp. di Pertanaman Cabai(Capsicum spp.) Melalui Teknik Uji Serologi dan Molekuler NI KADEK VENIARI; KETUT AYU YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Detection of Cucumber Mosaic Virus(CMV ) and Chilli Veinal Mottle Virus (ChiVMV) on Weed Commelina spp. In Cropping Chilli Pepper(Capsicumspp.) Through Serology and Molecular Test This study aims to determine the type of weed Commelina spp. which became an alternative host Cucumber mosaic virus (CMV) andChili veinal mottle virus (ChiVMV) in pepper, as well as the detection of CMV and ChiVMV which infects the weed Commelina spp. use molecular methods. DAS-ELISA (Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay) and molecular techniques through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) are used. Research activities include (1) Survey mosaic symptoms and disease incidence in the field; (2) Identification the type of weed that grows on pepper plants area; (3) Preservation of samples Commelina spp. weed and chili which virussymptomatic; (4) Test through DAS-ELISA serology; (5) Molecular detection by RT-PCR. The research proves that the weed Commelina spp. can be alternate hosts ChiVMV and CMV, also CMV and ChiVMV can infect weedsCommelina spp in chilicropping. DAS-ELISA test results show samples of the weed Commelina spp. and chili infected with Cucumber mosaic virus(CMV) and Chili veinal mottle virus (ChiVMV). RT-PCR technique successfully applyingDNA target fragment size of 657 bp and 900 bp for CMV and ChiVMV accordance with the specific primers used.   Keyword :Chili pepper, CMV, ChiVMV, weeds commelina
Co-Authors GEDE SUASTIKA I Dewa Nyoman Nyana KETUT AYU YULIADHI