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All Journal Jurnal Fitopatologi Indonesia Agrotrop : Journal on Agriculture Science Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Penelitian Tanaman Industri Jurnal Penelitian Tanaman Industri (Littri)

I Dewa Nyoman Nyana

Agricultural Biotechnology, Faculty Of Agriculture, Udayana University

Author-ID : 2452013

Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering Medicine & Pharmacology

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Laporan Pertama tentang Infeksi Polerovirus pada Tanaman Cabai di Daerah Bali, Indonesia Gede Suastika; Sedyo Hartono; I Dewa Nyoman Nyana; Tomohide Natsuaki
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

During a visit to Bali Island in September 2011, we found chilipepper plants exhibiting yellowing symptoms. The infected plants showed quite similar symptom to those of the recently reported Pepper yellow leaf curl virus from Israel and Japan, but there was no shortening of internodes or leaf rolling. We have ampliﬁed part of the genome of a virus associated with this disease using degenerate primers for members of the genus Polerovirus. The reverse transcription-polymerase chain reaction (RT-PCR) from symptom-bearing samples resulted in the amplification of a 650 bp band which is the expected size. RT-PCR from healthy samples did not produce an amplicon. Based on our results, we concluded that yellowing disease on chilipepper observed in Payangan, Gianyar, Bali is associated with a virus belonging to the genus Polerovirus. This is the first report on Polerovirus infection in chilipepper in Indonesia. Some researches for elucidation of the virus species and biological/molecular characters are being conducted.Key word: chilipepper, Indonesia, Polerovirus

Laporan Pertama Infeksi Begomovirus pada Tanaman Mentimun di Bali I Dewa Made Putra Wiratama; Gusti Ngurah Alit Susanta Wirya; I Dewa Nyoman Nyana; Ni Nengah Putri Adnyani; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Leaf yellowing symptoms was commonly found in cucumber plants in Bali provinces, i.e. in Apuan and Bangli villages recently. Begomovirus infection is suspected as the causal agent, due to similar symptoms previously reported from cucumber plants in Java. In addition, Bemisia tabaci was observed in the field. The objective of this research was to identify the causal agent of leaf yellowing disease of cucumber in Bali. Virus detection and identification was conducted by polymerase chain reaction method using universal primers for Begomovirus, i.e. SPG1/SPG2. DNA fragment of 912 bp in size was successfully amplified from leaf samples. Analysis of nucleotide sequencing indicated that Begomovirus infecting cucumber plants in Bali has the highest homology (91%) with Squash leaf curl China virus (SLCCNV) isolate from Malaysia. This is the first report of SLCCNV infection in Bali.

Identifikasi Pepper vein yellows virus yang Berasosiasi dengan Penyakit Yellow Vein Banding pada Tanaman Mentimun di Bali I Dewa Nyoman Nyana; M. Tinny Lestariningsih; Ni Nengah Putri Adnyani; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellowing vein banding disease has been reported infecting cucurbit plants in Bali since 2014. Similar vein banding symptom on chilli pepper was observed previously, and early diagnosis indicated infection of Polerovirus. The objective of this research was to confirm the presence of Polerovirus infection on cucumber plant showing yellow vein banding symptom in Bali. Reverse transcription polymerase chain reaction – based detection method was conducted using specific primer pairs PeVYV-CP-F-BamH1/ PeVYV-CP-R-Pst1followed by sequencing and nucleotide sequence analysis. Specific DNA fragments of ± 650 bp was successfully amplified from field samples. Nucleotide sequence analysis showed that the sequence has the highest similarity > 95% with Pepper vein yellow virus (PeVYV) infecting chili pepper from Indonesia (Bali, and Rembang), Japan, and Greece.

PENGARUH PENGGUNAAN BARIER NET BERWARNA (MERAH DAN PUTIH) TERHADAP POPULASI Bemisia tabaci PADA TANAMAN CABAI RAWIT (Capsicum frutescens L.) DI DESA KERTA Made Mika Mega Astuthi; I Putu Sudiarta; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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The research about impact of using barrier of net color (red and white) for whitefly population on cayenne pepper plant in Kerta Village was conducted in order to know firstly about species of whitefly that existing on the cayenne pepper, secondly to know about impact of net color for populations of whitefly on the cayenne papper. The research was done using the randomized block design (RBD) with 3 treatments and 9 replications. The observation covered of the population of whitefly and symptom of virus infection. The results of this research showed whitefly were identified as Bemisia tabaci. The result indicated the lowest average populations of Bemisia tabaci occurred in the white net barrier treatment.

IDENTIFIKASI SPESIES POTYVIRUS PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI (Capsicum frutescens L.) MELALUI SIKUEN NUKLEOTIDA GEN Coat Protein I Gede Rian Pramarta; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Chili peppers (Capsicum frutescens L.) is one of the important vegetable in the world and one of the leading horticultural commodities in Indonesia. Pepper plants grown in all provinces in Indonesia and got priority for development because it has great potential economic value. These researches was conducted in order to determine the type of infecting Potyvirus on chili and know the proximity nucleotide sequence of Potyvirus infecting chili crop in Kerta village, Payangan, Gianyar, Bali, with the kind of Potyvirus in other areas. This study use RT-PCR technique to detect plant viruses and continued with tracing sequence coat protein gene nucleotide to its proximity to some of the isolates analyzed from GeneBank. The results showed that the infecting Potyvirus chili crop is Chili veinal motle virus species (ChiVMV) with size of approximately 900 bp DNA band, and based on phylogenetic analysis of coat protein gene nucleotide sequence, PayanganIndo isolate had high similarity (93%) and small genetic distance (0.041) with isolate of origin Thailand state.

PENGARUH DRY HEAT TREATMENT TERHADAP DAYA SIMPAN BENIH CABAI RAWIT (Capsicum frutescens L.) I Gusti Ngurah Raka; Anak Agung Made Astiningsih; I Dewa Nyoman Nyana; I Ketut Siadi
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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The aims of this experiment were to compare the shelf life of chili pepper seeds given dry heat treatment (DHT) and non dry heat treatment (NT). This experiment was conducted in Laboratory of Seed Technology Faculty of Agriculture, Udayana University. The study consisted of two experiments that attempt to use lots of chili seeds treated with dry heat treatment (DHT) and experimental use a lot of chili seeds without dry heat treatment (NT). This experiment uses a randomized completely design (RCD) with 10 replications. Each type of chili pepper seeds tested each power shelf with 10 replications. Storage treatment performed at room temperature, which consists of non-storage (T0), storage for 2 weeks (T2), storage for 4 weeks (T4), storage for 6 weeks (T6) and storage for 8 weeks (T8). Thus, for each lots of chili pepper there are 50 experimental units. Treatment until 8 weeks of storage time did not affect the viability and vigor of pepper seeds either DHT or NT with value up to 80%, so they do not affect seed longivity. The dry heat treatment (DHT) showed the values of seed and seedling vigor are higher compared to that of no dry heat treatment (NT).

Pengaruh Penggunaan Mulsa Plastik Terhadap Kelimpahan Serangga Myzus persicae pada Tanaman Cabai Rawit (Capsicum frutescens L.) KADEK DWI UTAMA; I GUSTI NGURAH BAGUS; I KETUT SIADI; DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effect of Plastic Mulch to Myzuspersicae Insect Abundance in Plant Cayenne Pepper (Capsicum frutescens L.) Cayenne Pepperis one of theimportantvegetablesgrowncommerciallyinthe tropicsandoccupythe mostextensiveacreageamongother vegetablesin Indonesian. There are fivespeciesarecommonly cultivatedchiliCapsicumannuum(red pepper), C.frutescensL(cayenne pepper), andC.chinensis, C.bacctum, C.pubescens(chili gendot). However,theeconomicpotentialis a typeof chiliC.annuumandC.frutescensL. The purposeofthis study wastoknow theeffect of the useof plasticmulchoninsectabundanceMyzus persicaeoncropsandcayenne pepper. There arethree treatmentsbeing testedareplantingchiliusingblackplasticmulch(MH), a silverplasticmulch(MP) andplantingwithoutmulchorcontrol(K).The results showedthat theuse ofblack plasticmulchandsilverplasticmulchcannotpreventaphids andwhiteflyinchillicrop, however the presence ofblackplasticmulchandsilverplasticmulchcanreduceboththe insectpopulation. Key words :Cayenne Pepper,Myzus persicae, plastic mulch

Pengaruh Penggunaan Mulsa Plastik terhadap Hasil Tanaman Cabai Rawit (capsicum frutescens l.) di Luar musim di Desa Kerta I GEDE PUTU DARMAWAN; I DEWA NYOMAN NYANA; I GUSTI ALIT GUNADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Influence of Plastic Mulch on Crop Chili Pepper (Capsicum frutescens L.) Off Season in the Kerta village. Chili is one of the important horticultural commodities in Indonesia and has a high economic value. To maintain its supplies, cultivation technology that able to produce the commodities continuously is needed by applying technology in the off-season cultivation. This study aims to determine the effect of plastic mulch on chili crop in the off-season. The experimental design was used a randomized block design with 3 treatments and 9 replications. The treatment given is the use of mulch consisting of silver plastic mulch (MP), black plastic mulch (MH), and without mulch/control (K). The variables observed in this study, ware, plant height, number of branches, soil temperature, symptoms of viral diseases, and yield per hectare. The results showed the plastic mulch give good results on chili crop in the off-season. However, the highest results of the chili plants was in the silver plastic mulch treatments with plant height (95.64 cm), number of branches (13.11 piece), and yield (12.31 ton/ha). Keywords: chili pepper, off season, plastic mulch, yield

Toleransi Penundaan Prosesing Terhadap Mutu Fisik dan Mutu Fisiologis Benih Kedelai (Glycine max L. Merril) ROBERTO BOBHOPE ARUAN; I DEWA NYOMAN NYANA; I KETUT SIADI; I GUSTI NGURAH RAKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Tolerance of Processing Delay to Physical and Physiological quality of Soya Bean (Glycine max L. Merril) The aim of this research is to figure out the time of the tolerance processing delay so that the physical quality and the physiological quality of soya bean seeds could be defended. This research was started in April 2017 at Jalan Cekomaria, Denpasar and also at the Lab. Pemuliaan Tanaman dan Teknologi Benih Faculty of Agriculture Udayana University. The design method was used for this research is a randomized complete block design (RCBD) using five treatment and five-time repetitions consist of W0= Without using process, W1= 1 day of Delaying process W2= 2 days of Delaying process, W3 = 3 days of Delaying process, W4 = 4 days of Delaying process. The statistic test and analyzing variance statistical test shows different result, the procedure followed by LSD method (Least Significance Different) with 95% of confidence level using costat program.The result of physical analysis test, shows that the role of the seeds which is without delaying process showing that the percentage of the seeds was 87,36% and the result of 4 days delaying process seeds are 11,256%. The result of the physiological test of the seeds using germinating test was, W0 (95,16%) and W4 (94,72%). The percentage of the vigor will be decreasing, which is W0 (80,52%) and W4 (34,4%). The equality control of the percentage was W0 (90,3%) and W4 (70,68%). Result of the observation of electrical conductivity showing that the lowest point of the electrical conductivity control is W0 (5,112 ? mhos cm¯² g¹) and the highest value of electrical conductivity was shown on the W4 (7,302 ? mhos cm¯² g¹).

Pengaruh Penyakit Virus Mosaik dan Kuning Terhadap Hasil Panen Tanaman Kacang Panjang (Vigna sinensis L.) Di Desa Perean, Baturiti, Tabanan NI NYOMAN ALIT PURWANINGSIH; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.3, Juli 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Influence Mosaic Diseases and Yellow to Long Bean (Vigna sinensis L.)Production at Perean Village, Baturiti, TabananThis study aims to determine how far the influence of viral infection with symptoms of mosaic and yellow against a decrease in crop yield long beans. This research consists of four activities are: 1. Determining the spread of disease areas; 2. Observation and data collection; 3. Calculation yields; and 4. Data analysis. Design used was a randomized block design (RBD) with three treatments and nine replications.Research result show that long bean plants in several locations in Perean village infected by BCMV which has mosaic symptoms and MYMV which has yellow symptoms. Percentage of mosaic virus symptoms is higher than yellow virus. The average of percentage of mosaic virus symptoms was 42,85%, while yellow virus was 32,70%. Plant beans with yellow virus symptoms causes the highest yield loss which amounted to 53,87% and plants with symptoms of mosaic virus by 43,02% when compared with healthy plants. Based on these studies it is necessary dissemination of information, about the importance of sanitation and the use of healthy seeds to controlling the long bean’s viral infection to related parties.

Co-Authors A. A. GEDE PUTRA ADHITYA ANAK AGUNG MADE ASTININGSIH AYU GEK MIRAH LESTIANINGRUM DWI OCKVIAN ANESTA Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gusti Ngurah Alit Susanta Wirya I Dewa Made Putra Wiratama I G. R. M. TEMAJA I GEDE PUTU DARMAWAN I Gede Rian Pramarta I GUSTI ALIT GUNADI I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I GUSTI NGURAH RAKA I KADE DARMAWAN I Ketut Siadi I MADE BAGUS ANGGARA I MADE SUDANA I MADE SUDANA I NYOMAN WIJAYA I WAYAN SUKADA IDA BAGUS GDE PRANATAYANA KADEK DWI UTAMA KETUT AYU YULIADHI Khamdan Khalimi M. Tinny Lestariningsih Made Mika Mega Astuthi Miftakhurohmah Miftakhurohmah NI KADEK VENIARI NI LUH MADE PRADNYAWATHI NI LUH PUTU CITRA INNOSENSIA Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ALIT PURWANINGSIH NI NYOMAN DIAH UTTARI NI NYOMAN TRISNA KENCANA DEWI NI PUTU EKA HANDAYANI NYOMAN AYU TRISNA ARI UTAMI Putu Sudiarta Rita Noveriza ROBERTO BOBHOPE ARUAN SEDYO HARTONO Tomohide Natsuaki TRI ASMIRA DAMAYANTI

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