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GEDE SUASTIKA
IPB-Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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Pengaruh Penggunaan Mulsa Plastik Terhadap Kelimpahan Serangga Myzus persicae pada Tanaman Cabai Rawit (Capsicum frutescens L.) KADEK DWI UTAMA; I GUSTI NGURAH BAGUS; I KETUT SIADI; DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effect of Plastic Mulch to Myzuspersicae Insect Abundance in Plant Cayenne Pepper (Capsicum frutescens L.)   Cayenne Pepperis one of theimportantvegetablesgrowncommerciallyinthe tropicsandoccupythe mostextensiveacreageamongother vegetablesin Indonesian. There are fivespeciesarecommonly cultivatedchiliCapsicumannuum(red pepper), C.frutescensL(cayenne pepper), andC.chinensis, C.bacctum, C.pubescens(chili gendot). However,theeconomicpotentialis a typeof chiliC.annuumandC.frutescensL. The purposeofthis study wastoknow theeffect of the useof plasticmulchoninsectabundanceMyzus persicaeoncropsandcayenne pepper. There arethree treatmentsbeing testedareplantingchiliusingblackplasticmulch(MH), a silverplasticmulch(MP) andplantingwithoutmulchorcontrol(K).The results showedthat theuse ofblack plasticmulchandsilverplasticmulchcannotpreventaphids andwhiteflyinchillicrop, however the presence ofblackplasticmulchandsilverplasticmulchcanreduceboththe insectpopulation. Key words :Cayenne Pepper,Myzus persicae, plastic mulch
Identifikasi Virus Yang Berasosiasi Dengan Penyakit Mosaik, Kuning, Dan Klorosis Pada Tanaman Cabai Rawit (Capsicum Frutescens L.) I GUSTI NGURAH BAGUS PRANATA PUTRA; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA; I KETUT SIADI; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Identification of virus that associated with Mosaic, Yellow, and Chlorosis disease on Chili Pepper (Capsicum frutescens L) This study aims to identify the types of viruses associated with mosaic, yellow, and chlorosis that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, Gianyar. The method used is the ELISA (Enzyme Linked Immunosorbent Assay) and molecular techniques through PCR (Polymerase Chain Reaction). Research activities include (1) Survey endemic locations of viral diseases in Kerta village, Gianyar (2) Collecting leaf of chilli peper that shown, mosaic, yellowing and chlorosis (3) serology test by ELISA (4) molecular detection through PCR. The results show the percentage average of pepper plants showing mosaic symptoms (52.13%), yellow (22.75%), chlorosis (5.45%) and healthy plant (19.67%). Serology test by ELISA technique showed that the mosaic disease induced by the triple virus that is TMV, ChiVMV, and CMV, whereas yellow symptoms infected by PepYLCV and chlorosis symptoms, infected by Polerovirus. RT-PCR technique successfully amplify the target DNA fragment size of 650 bp for Polerovirus and PCR successfully amplified the target DNA fragment size of 700 bp for PepYLCV in accordance with the specific primers were used. Keywords : Chili pepper, mosaic , yellow, chlorosis
Kisaran Inang Bean Common Mosaic Virus (Bcmv) Penyebab Penyakit Mosaik Pada Tanaman Kacang Panjang (Vigna sinensis L.) A. A. GEDE PUTRA ADHITYA; I GEDE RAI MAYA TEMAJA; NI NENGAH DARMIATI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Host range of Bean Common Mosaic Virus (BCMV) causal agent of mosaic diseases on long yard bean  (Vigna sinensis L.) This objective of the study is order to find out the host range of BCMV and symptom variation. DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) menthod was used in this study. The study consisted of (1) Host range evaluation by mechanical inoculation, (2). Symptom observation on tested plants, (3) Serological test with DAS–ELISA. The result indicated that BCMV can infected Leguminoceae plants (peanuts, long yard beans, and beans); Solanaceae plant (tomatoes); Cucurbetaceae plant (cucumbers); Amaranthaceae plant (spinach); and Balsaminaceae plant (henna flowers). DAS-ELISA analysis shown that those plants were infected by BCMV. The symptoms variation were mosaic, vein banding,  wrinkled, and curved on commercial long yard beans (aura and panah merah varieties); wrinkled on commercial long yard beans (KPK and pusaka hijau varieties), cucumber and spinach; wrinkled and rolled on beans; wrinkled and curved upside on henna flowers.   Key word : bean common mosaic virus ,  long yard beans, DAS-ELISA
Deteksi Molekuler Cucumber Mosaic Virus (Cmv) pada Tanaman Gamal (Gliricidia Sepium) Sebagai Barier pada Pertanaman Cabai IDA BAGUS GDE PRANATAYANA; I GEDE RAI MAYA TEMAJA; KETUT YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Molecular Detection of Cucumber Mosaic Virus (CMV) In Gliricidia Plant (Gliricidia sepium) as Barrier at Chili pepper Crop The aims of the study are in order to symptoms characteristic identity the CMV infection on gliricidia plant and symptoms characteristic the CMV infection on chili pepper plant, and order to presence identity the CMV on gliricidia plant and presence the CMV on chili papper plant at Kerta village base on molecular analysis. The serology method was used to detect CMV virus is DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) was used for molecular analysis. The study include 1) the observation of mosaic in field, 2) preservation of plant samples (chili pepper and gliricidia) infected with mosaic virus, 3) serology test with DAS-ELISA, 4) molecular detection with RT-PCR. The research proved that symptoms characteristic the CMV infection on gliricidia plant identical with symptoms characteristic the CMV infection on chili pepper plant and presence the CMV on gliricidia plant identical with presence the CMV on chili pepper plant. symptoms of mosaic characteristic in gliricidia and chili pepper found on the field among others, mosaic (striped), contract and roll with variations of symptoms that light green mosaic with chlorotic and dark green mosaic symptoms in gliricidia and chili pepper plants. DAS-ELISA test results showed gamal and chili pepper samples infected positive of Cucumber Mosaic Virus (CMV). RT-PCR technique successfully amplify the target DNA fragment size of 657 bp. Based on these studies, the farmers need to be aware of the existence of the plant used as a plant barrier on chilli crop, because gliricidia potential as alternative hosts of CMV.   Key word : Chili pepper, cucumber  mosaic virus, gliricidia, molecular
Deteksi Cucumber Mosaic Virus (CMV) dan Chili Veinal Mottle Virus (ChiVMV) pada Gulma Commelina spp. di Pertanaman Cabai(Capsicum spp.) Melalui Teknik Uji Serologi dan Molekuler NI KADEK VENIARI; KETUT AYU YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Detection of Cucumber Mosaic Virus(CMV ) and Chilli Veinal Mottle Virus (ChiVMV) on Weed Commelina spp. In Cropping Chilli Pepper(Capsicumspp.) Through Serology and Molecular Test This study aims to determine the type of weed Commelina spp. which became an alternative host Cucumber mosaic virus (CMV) andChili veinal mottle virus (ChiVMV) in pepper, as well as the detection of CMV and ChiVMV which infects the weed Commelina spp. use molecular methods. DAS-ELISA (Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay) and molecular techniques through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) are used. Research activities include (1) Survey mosaic symptoms and disease incidence in the field; (2) Identification the type of weed that grows on pepper plants area; (3) Preservation of samples Commelina spp. weed and chili which virussymptomatic; (4) Test through DAS-ELISA serology; (5) Molecular detection by RT-PCR. The research proves that the weed Commelina spp. can be alternate hosts ChiVMV and CMV, also CMV and ChiVMV can infect weedsCommelina spp in chilicropping. DAS-ELISA test results show samples of the weed Commelina spp. and chili infected with Cucumber mosaic virus(CMV) and Chili veinal mottle virus (ChiVMV). RT-PCR technique successfully applyingDNA target fragment size of 657 bp and 900 bp for CMV and ChiVMV accordance with the specific primers used.   Keyword :Chili pepper, CMV, ChiVMV, weeds commelina
Co-Authors A. A. GEDE PUTRA ADHITYA I Dewa Nyoman Nyana I G. R. M. TEMAJA I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I Ketut Siadi IDA BAGUS GDE PRANATAYANA KADEK DWI UTAMA KETUT AYU YULIADHI NI KADEK VENIARI Ni Made Puspawati NI NENGAH DARMIATI