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All Journal Dentika Dental Journal Journal of Dentistry Indonesia
Yulianti Kemal
Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430
Dentistry

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QUANTIFICATION OF STREPTOCOCCUS SANGUINIS ISOLATED FROM DENTAL PLAQUE AND SALIVA OF SUBJECTS WITH AND WITHOUT CORONARY HEART DISEASE – ANALYSIS USING REAL-TIME PCR: KUANTIFIKASI STREPTOCOCCUS SANGUINIS YANG DIISOLASI DARI PLAK DAN SALIVA GIGI SUBYEK DENGAN DAN TANPA PENYAKIT JANTUNG KORONER DENGAN MENGGUNAKAN PCR REAL-TIME Nasution, Aini Hariyani; Kemal, Yulianti; Lessang, Robert; Bachtiar, Boy
Dentika: Dental Journal Vol. 20 No. 1 (2017): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.018 KB) | DOI: 10.32734/dentika.v20i1.645

Abstract

Coronary Heart Disease (CHD) is the major cause of death in most countries in the world. Gram-positive and Gram-negative bacteria have been identified in bacteremia cases and known to have a role in various vascular diseases, including Streptococcus sanguinis which is most frequently isolated from endocarditis patients and often associated with CHD. The purpose of this study was to analyze the number of Streptococcus sanguinis isolated from dental plaque and saliva of subjects with and without CHD. Bacterial colonies isolated from the dental plaque and saliva of 16 subjects without CHD and 8 subjects with CHD were planted in Mitis salivarius agar, and then the DNA was extracted and quantified with a Real-Time PCR technique using 16S rRNA specific primers. The quantification of Real-Time PCR showed that there was a difference in the number of S. sanguinis between the two groups of subjects, but an unpaired T-test showed that the difference was not statistically significant. Furthermore, the number of S. sanguinis from dental plaque in CHD subjects tends to be higher than that of non-CHD subjects whereas the number of S. sanguinis from saliva in non-CHD subjects tends to be higher than that of CHD subjects.
Academic Stress Influences Periodontal Health Condition and Interleukin-1 beta Level Kuswandani, Sandra O.; Masulili, Sri LC.; Soedarsono, Nurtami; Kemal, Yulianti
Journal of Dentistry Indonesia
Publisher : UI Scholars Hub

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Abstract

Stress is a risk factor for periodontal disease, causing increase levels of interleukin-1 beta that involve in periodontal destruction. Objective: To analyze the relationship between academic stress in residency program students conditions and levels of interleukin-1 beta in gingival crevicular fluid. Methods: Thirty eight subjects filled the questionnaire of Graduate Dental Environtmental Stress (GDES), periodontal examination and samples of gingival crevicular fluid were tested for interleukin-1 beta with the Enzyme-linked Immunosorbent Assay (ELISA) test. Results: There were significant differences between academic stress to periodontal tissue in oral hygiene (p=0.038), bleeding on probing index (p=0.02), but no significant differences in pocket depth and loss of attachment (p=0.972). There were significant differences between academic stress to levels of interleukin-1 beta (p=0.03), but no significant differences between levels of interleukin-1 beta to periodontal tissue in oral hygiene (p=0.465), bleeding on probing index (p=0.826), pocket depth (p=0.968), and loss of attachment (p=0.968). Conclusion: Academic stress influences the periodontal risk factor and level of interleukin-1 beta.
Adjunctive Intracoronal Splint in Periodontal Treatment: Report of Two Cases Octavia, Mora; Soeroso, Yuniarti; Kemal, Yulianti; Airina, Airina
Journal of Dentistry Indonesia
Publisher : UI Scholars Hub

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Abstract

Reduction of the amount of tooth roots which are embedded in their periodontium could cause tooth mobility. Splinting a weaker tooth with a more stable one, and using the principle of the multiple-root stabilization is one way to overcome tooth mobility. Temporary splinting aims to prevent pathological migration, restore masticatory function, stabilize teeth before/after surgery, and evaluate the prognosis of periodontal treatment. The use of intracoronal splint is still controversial because there are only a few studies that have evaluated the effect of splinting on periodontal health. We report two cases to evaluate the effect of intracoronal splint on periodontal treatment. Two periodontal cases that use intracoronal splint before, during, and after periodontal regenerative therapy using bone graft. Causes of tooth mobility were removed and the splinting principles, terms and guidelines were mastered to get the maximum results of periodontal treatment. Both cases were evaluated radiographically 10 months after treatment. In these cases, intracoronal splint has supported the therapy before, during, or after surgery. Splinting is only for adjunctive therapy, and does not serve as the sole method in getting occlusal stability.
Co-Authors Airina Airina Boy Bachtiar Boy Muchlis Bachtiar Lukman Hakim Makmun Masulili, Sri LC. Mora Octavia Nasution, Aini Hariyani Natalina , Nurtami Soedarsono Robert Lesang Robert Lessang Sandra O. Kuswandani, Sandra O. Yuniarti Soeroso