Winarsa, Rudju
Biology Department, Faculty Of Mathematic And Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, Indonesia, 68121

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Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Khofiya, Zunairoh Nidaan; Winarsa, Rudju; Muzakhar, Kahar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9915

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml. Kata Kunci: Kulit buah kopi, hidrolisis, produksi PST, Pestalotiopsis sp.
PRODUKSI GUM ARABIC BALURAN SEBAGAI PENDUKUNG PENGEMBANGAN WISATA KAMPUNG BANTENG DI KARANG TEKOK SEBAGAI WILAYAH PENYANGGA TN BALURAN Kusumah, Maulana S.; Wiyono, Hidayat Teguh; Subekti, Agus; Muzakhar, Kahar; Winarsa, Rudju
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 4 No 1 (2020): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Forum Dosen Indonesia JATIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v4i1.272

Abstract

This article is the result of PPDM (Mitra Desa Service Program) about Baluran bos javanicus (Banteng) village tourism. Development of bull village tourism is an effort to solve the problem of wild grazing in Bunaken National Park. In the event of Banteng Village Tourism, it is necessary to support tourism, namely creative industries, agro-tourism, and NTFP production (non-timber forest products). One of the NTFPs that is relied upon is Arabic gum. Currently, cattle breeders in the banteng village area have been able to produce Arabic gum as a result of the introduction of tapping technology by the 2019 PPDM team. The dedication method is in the form of dissemination and field practice. Three groups representing breeders were trained to tap acacia gum through a drilling method combined with ethephon induction as GIS. One week after application, the group begins harvesting gum and submits the results to the group leader. Then the group leader sends the results to the Cooperative in Pondok Pesantren Assalam, Sumberanyar, Banyuputih Situbondo. The amount of Baluran Arabic gum that was collected by the group for three months reached 143.9 kg. This service activity concludes that the strength in producing Baluran Arabic gum is significant in improving the welfare of breeders in supporting the maintenance and retention of a Banteng.
Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes Kahar Muzakhar; Rudju Winarsa
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4411

Abstract

 An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. 
Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Zunairoh Nidaan Khofiya; Rudju Winarsa; Kahar Muzakhar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9682

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml.
STRUKTUR KOMUNITAS FITOPLANKTON PADA ZONA LITORAL RANU PAKIS Abdur Rasit; Moh. Imron Rosyidi; Rudju Winarsa
BERKALA SAINSTEK Vol 4 No 1 (2016)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Struktur komunitas fitoplankton merupakan bentuk dari masing-masing penyusun komunitas, antara lain komposisi, kelimpahan dan keanekaragaman jenis. Tujuan penelitian ini adalah untuk mengetahui komposisi, kelimpahan dan keanekaragaman jenis fitoplankton pada zona litoral Ranu Pakis. Hasil pengamatan menggunakan mikroskop menunjukkan fitoplankton yang terdapat pada zona litoral Ranu Pakis terdiri atas 4 kelas. Kelas tersebut yaitu yaitu Bacillariophyceae (7 jenis), Chlorophyceae (8 jenis), Cyanophyceae (5 jenis) dan Dinophyceae (1 jenis). Kelas Cynophyceae merupakan kelas yang paling melimpah dengan jumlah jenis sebanyak 231555 ind/L. Jenis yang mendominasi adalah Chroococcus sp. yang merupakan jenis dari kelas Cynophyceae dengan nilai indeks dominansi sebesar 0,6. Melimpah dan mendominasinya kelas Cyanophyceae disebabkan karena dari hasil pengukuran kondisi fisika dan kimia yang mendukung pertumbuhan Cyanophyceae. Dominansi Chroococcus sp. berdampak pada nilai keanekaragaman jenis fitoplankton di Ranu Pakis yaitu sebesar 1,01.
Aktivitas Antioksidan Kedelai Edamame Hasil Fermentasi Kultur Campuran oleh Rhizopus oligosporus dan Bacillus subtilis Tutus Ervian Ningsih; S. Siswanto; Rudju Winarsa
BERKALA SAINSTEK Vol 6 No 1 (2018)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v6i1.7556

Abstract

Kedelai edamame mengandung senyawa isoflavon yang berfungsi sebagai antioksidan. Senyawa tersebut berupa senyawa yang berikatan dengan gula melalui ikatan glikosida. Biokonversi senyawa isoflavon glikosida menjadi isoflavon aglikon yang sangat berpotensi tinggi sebagai antioksidan terjadi selama proses fermentasi oleh aktivitas enzim β-glukosidase. Dilaporkan enzim β-glukosidase dihasilkan oleh Rhizopus spp. Selain itu, selama proses fermentasi Bacillus subtilis menghasilkan enzim nattokinase yang juga dapat meningkatkan aktivitas antioksidan. Pada penelitian ini proses fermentasi dilakukan menggunakan inokulum Rhizopus oligosporus, B. subtilis serta campuran keduanya dengan lama fermentasi 24 jam, 48 jam dan 72 jam. Kemudian aktivitas antioksidan diuji menggunakan larutan DPPH (1,1-difenil-2-pikrilhidrazil). Proses fermentasi pada pembuatan tempe edamame dengan inokulum R. oliosporus dan B. subtilis dapat meningkatkan aktivitas antioksidan eksogenous. Aktivitas antioksidan eksogenous tertinggi oleh R. oligosporus sebesar 97% pada fermentasi 72 jam. Kata Kunci: Kedelai edamame, Isoflavon aglikon, DPPH
Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Fitri Azhari; Rudju Winarsa; Siswanto Siswanto; Kahar Muzakhar; Esti Utarti; Sutoyo Sutoyo; Sattya Arimurti
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Abstract

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.
TOKSISITAS EKSTRAK EKSTRAKSI SERBUK GERGAJI KAYU SENGON LAUT (Albizia falcataria L. Forberg) TERHADAP MORTALITAS Hypothenemus hampei Ferr. (COLEOPTERA: SCOLITYDAE) Paramita Pratiwi; Rudju Winarsa; Purwatiningsih Purwatiningsih
Jurnal Pro-Life Vol. 6 No. 2 (2019): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract This study was conducted to determine the toxicity of methanol extract SengonSengon SengonSengonSengon wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust wood sawdust againstagainstagainstagainst againstagainst Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different con Hypothenemus hampei. There were 6 different conHypothenemus hampei. There were 6 different concentration centrationcentration centrationcentration centrations of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of extract tested to insect by using contact method. Ten of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 of H. hampei were used for each concentration and replicated 10 times. Mortality insects was recorded 24, 48 and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te and 72 hours. The data was analyzed using GLM (General Linear Model) teand 72 hours. The data was analyzed using GLM (General Linear Model) te st and st and st and st and st and st and Duncan DuncanDuncanDuncanDuncan test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence test at 95% confidence level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of level. Results showed that methanol extract of SengonSengon SengonSengonSengon wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. wood sawdust had significant effect on the mortality of H. hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of hampei after 72 hours. It can be concluded that methanol extract of SengonSengon SengonSengonSengon wood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase thewood sawdust could increase the wood sawdust could increase the wood sawdust could increase thewood sawdust could increase the wood sawdust could increase thewood sawdust could increase the wood sawdust could increase the wood sawdust could increase the wood sawdust could increase thewood sawdust could increase the wood sawdust could increase the wood sawdust could increase the mortality of H. hampei. mortality of H. hampei.mortality of H. hampei. mortality of H. hampei. mortality of H. hampei. mortality of H. hampei.mortality of H. hampei.mortality of H. hampei.mortality of H. hampei.mortality of H. hampei. mortality of H. hampei.Keywords: Hipotenemus hampei, mortality, Sengon wood sawdust, toxcicity.
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Kahar Muzakhar; Farah Salma Elida; Ramdhan Putrasetya; Siswoyo Siswoyo; Rudju Winarsa; Hidayat Teguh Wiyono
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.