Article Per Year (5 Year)
p-Index From 2019 - 2024
1.431
P-Index
This Author published in this journals
All Journal International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar IPTEK Journal of Proceedings Series BERKALA SAINSTEK Biotropika Journal of Mathematical and Fundamental Sciences Jurnal Biodjati JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Kahar Muzakhar
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Jember University
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Physics Public Health Other

Published : 16 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 16 Documents
Search

 1   2 
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Khofiya, Zunairoh Nidaan; Winarsa, Rudju; Muzakhar, Kahar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9915

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml. Kata Kunci: Kulit buah kopi, hidrolisis, produksi PST, Pestalotiopsis sp.
PRODUKSI GUM ARABIC BALURAN SEBAGAI PENDUKUNG PENGEMBANGAN WISATA KAMPUNG BANTENG DI KARANG TEKOK SEBAGAI WILAYAH PENYANGGA TN BALURAN Kusumah, Maulana S.; Wiyono, Hidayat Teguh; Subekti, Agus; Muzakhar, Kahar; Winarsa, Rudju
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 4 No 1 (2020): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Forum Dosen Indonesia JATIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v4i1.272

Abstract

This article is the result of PPDM (Mitra Desa Service Program) about Baluran bos javanicus (Banteng) village tourism. Development of bull village tourism is an effort to solve the problem of wild grazing in Bunaken National Park. In the event of Banteng Village Tourism, it is necessary to support tourism, namely creative industries, agro-tourism, and NTFP production (non-timber forest products). One of the NTFPs that is relied upon is Arabic gum. Currently, cattle breeders in the banteng village area have been able to produce Arabic gum as a result of the introduction of tapping technology by the 2019 PPDM team. The dedication method is in the form of dissemination and field practice. Three groups representing breeders were trained to tap acacia gum through a drilling method combined with ethephon induction as GIS. One week after application, the group begins harvesting gum and submits the results to the group leader. Then the group leader sends the results to the Cooperative in Pondok Pesantren Assalam, Sumberanyar, Banyuputih Situbondo. The amount of Baluran Arabic gum that was collected by the group for three months reached 143.9 kg. This service activity concludes that the strength in producing Baluran Arabic gum is significant in improving the welfare of breeders in supporting the maintenance and retention of a Banteng.
Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes Kahar Muzakhar; Rudju Winarsa
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4411

Abstract

 An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. 
Purification and Characterization of Cellulase of Mold Isolated from Vermicomposting Process of Palm Oil Empty Fruit Bunches Rosita Fitrah Dewi; Kahar Muzakhar
Jurnal Biodjati Vol 3, No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2292

Abstract

In previous investigation of vermicomposting process of palm oil empty fruit bunches, five cellulase-producing microorganisms had been isolated. An isolated VTM1 identified as Aspergillus sp. as a cellulase producing mold, and proven produced extracellular cellulase in solid state fermentation of palm oil empty fruit bunches. The obtaining crude cellulase was purified to homogeneity by ammonium sulfate precipitation, gel filtration on Sephadex G-100 chromatography, and finally purified on ion exchange chromatography on DEAE Cellulofine A-500. The yield and purification fold were 12.89% and 107.50, respectively. The purified cellulase was easily released glucose when 5% CMC applied as substrate, as shown by TLC analysis. The purified cellulase had an optimal pH and temperature at 4.0 and 45°C, and was stable at pH 3-7 and 30-50°C, respectively.
Skrining dan Identifikasi Bakteri Pektinolitik Endosimbion dalam Sistem Pencernaan Serangga Penggerek Kopi (Hypothenemus hampei Ferr) Gatot Kusiyanto; Purwatiningsih Purwatiningsih; Kahar Muzakhar
Biotropika: Journal of Tropical Biology Vol 7, No 2 (2019)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2019.007.02.1

Abstract

Serangga Hypothenemus hampei memiliki kemampuan untuk menggerek biji kopi, dan digunakannya sebagai sumber makanan, tempat hidup dan siklus hidup. Aktivitas hidup dari  H. hampei menyebabkan rontoknya buah kopi dan penurunan kualitas serta kuantitas buah kopi hingga 40%. Penelitian ini bertujuan untuk mendapatkan isolat bakteri pektinolitik yang memiliki toleransi tinggi terhadap lingkungan khususnya kafein, tanin dan polifenol. Prosedur penelitian terdiri dari tahap pembuatan media skrining bakteri pektinase, isolasi bakteri endosimbion dan identifikasi spesies serta fisiologi bakteri. Berdasarkan hasil identifikasi dan skrining bakteri pada sistem pencernaan H. hampei didapatkan 15 isolat bakteri pektinolitik endosimbion. Hasil identifikasi tiga isolat terpilih diketahui bahwa isolat 2B termasuk golongan Enterobacter sp. atau Actinobacillus sp., isolat 31B masuk ke dalam Micrococcus sp. dan isolat 42B termasuk Chromobacterium sp. Penelitian ini berguna sebagai kajian untuk strategi penanganan hama serangga H. hampei dengan menghambat pertumbuhan bakteri pektinolitik.
SUGAR PRODUCTION BY DIGESTING OF OIL PALM EMPTY FRUIT BUNCH USING EXTRACELLULAR ENZYMES FROM Aspergillus niger AND Trichoderma reesei FOR ETHANOL PRODUCTION Kahar Muzakhar; Sutoyo S; Siswoyo S
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.426 KB)

Abstract

Extracellular enzymes which obtained from 4 days cultivation Aspergillus niger and Trichoderma reesei onsolid state fermentation of oil palm empty fruit bunch (OPEFB) were used for lignocellulosic-­??rich OPEFBdigestion. The enzymes were concentrated using 70% saturated ammonium sulphate, dialysed against20mM acetate buffer at pH 5 and adjusted one tenth (v/v) from the initial volume with the same buffer.The concentrated enzymes were then used in hydrolysation of powdered OPEFB. Amount of 10.65 mg/ mland 11.47 mg/ml sugars were produced when each concetrated enzyme A. niger and T. reesei mixedwith2%OPEFB. These hydrolysation were done on 100 ml total volume, incubated at 37oC with 100 rpmshaken for 36 hours. Further, both hydrolyzates results were sterilised and fermented anaerobically usingSaccharomycess cerevisiae at concentration 0.5mg/ml cells and incubated in 30oC for 24 hours. Colorimetricanalysis using QuantiChrom Kit DIET-­??500 at OD 580nm gave results the alcohol production were 0.86%and 0.92% which were similar with Gas Chromatograph analysis that of 0.83% and 0.93%, respectively.
GROWTH PROFILES AND ETHANOL PRODUCTION OF TWO YEAST STRAINS Kluyveromyces marxianus IFO-288 AND Kluyveromyces marxianus IFO-617 DURING FERMENTATION OF SOYBEAN PULP HYDROLYZATE SUBSTRATES Kahar Muzakhar
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.136 KB)

Abstract

Two yeast strains, K.marxianus IFO-288 and K.marxianus IFO-617, were observed to utilize carbon andnitrogen sources obtained from enzymatically hydrolyzed soybean pulp (hydrolyzate). Under aerobiccondition where 1.5% hydrolyzate was used, the maximum biomass of the two yeast yield reached 30%with the protein contents of 47-53%. The two yeast strains also fermented the soybean pulp hydrolyzateand produced alcohol when anaerobic condition was set. The alcohol production effi ciencies of the K.marxianus IFO-288 and K. marxianus IFO-617were 49% and 59.7%, respectively in the medium containing1.5% hydrolyzate. These values were noticed to decrease for the two strains when the concentration of thehydrolyzate was increased. At the concentrations of 2.5% and 5% hydrolyzate for examples, the alcoholproduction effi ciencies of the K.marxianus IFO-288 became 42.5% and 18.5%, respectively, while 45.6% and20.8%, respectively were recorded in the medium inoculated with K. marxianus IFO-617. At 10% hydrolyzateconcentration, none of the yeast strains grew or produced alcohol, indicating that some inhibitory substancesin hydrolyzates were present in the fermentation medium.
Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Zunairoh Nidaan Khofiya; Rudju Winarsa; Kahar Muzakhar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9682

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Siti Nur Azizah; Kahar Muzakhar; Sattya Arimurti
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
Co-Authors Agus Subekti Ahmad Bukhari Saragih Esti Utarti Farah Salma Elida Fitri Azhari Gatot Kusiyanto Hidayat Teguh Wiyono Kartika Senjarini Khofiya, Zunairoh Nidaan Kusumah, Maulana S. Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Ramdhan Putrasetya Rosita Fitrah Dewi S. Siswanto Sattya Arimurti Siswoyo Siswoyo Siswoyo Siswoyo Siti Nur Azizah Siti Nur Azizah, Siti Nur Sutoyo S Sutoyo Sutoyo Sutoyo Sutoyo Ummi Wasilah Winarsa, Rudju Zunairoh Nidaan Khofiya