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Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Machrus, Ali; Adrial, Adrial; Armiyanti, Yunita; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite, the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of IFN-γ and decreasing level of IL-4 in mice model of malaria after vaccination with salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei. Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV

Immunogenity of Protein Extract from Salivary Gland of Anopheles aconitus in Malaria Endemic Area Septiawan, Mahful; Budayatin, Budayatin; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Although malaria had ever been virtually eradicated from Indonesia but currently malaria is recognized as a serious re-emerging threat to public health. This disease is caused by malaria parasite which is transmitted to human host by Anopheles mosquitoes as main vector. It has been widely observed that saliva of mosquito that transmits disease contains several factors that could enhance pathogen infection. Therefore, it should be possible to control pathogen transmission by vaccinating the host against the molecule(s) in saliva that potentiate the infection. However, immunogenic specific component in mosquitoes vectors of Malaria has not yet been identified so far. The objective of this study are to analyze protein profile of SDS-PAGE and to know the immunogity the protein extract of salivary gland from potential vector of Malaria i.e. An. aconitus We used immunogenic reaction between salivary gland extract of these vectors against pool of human sera which were collected from endemic area. The reaction conducted by the dot-blot analyze. SDS-PAGE studies showed 15 major polypeptide bands of 284, 100, 84, 75, 66, 57, 53, 48, 45, 38, 33, 29, 15, 14, and 11 kDa. The dot-blot studies showed that the protein extract of salivary gland from An. aconitus are immunogenic.

Relationship Bali Cattle (Bos sondaicus Muller) and Banteng (Bos bibos d'alton) Approach Through The Craniometric Mahdi, Arya; Wiyono, Hidayat Teguh; Suratno, Suratno
Jurnal ILMU DASAR Vol 14 No 2 (2013)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Relationship can be seen from the similarity of the cow and bull. One method to find out about the phylogenetic relationship between species is by using cranial morphometry (craniometrics). The purpose of this research was to identify morphological cranium Bali cattle , banteng baluran , and banteng merubetiri based on the parameters measured .This research was conducted in August and ending in October 2013 , held at the National Park Baluran and Merubetiri National Park. The design of this research is descriptive quantitative research , using data analysis kalster ( cluster analysis). Parameters of this research using 15 indicators that have been established in accordance with the research Hayashi , 1982. Materials used in this study are adult male cranium Bali cattle obtained from abattoir ( Slaughterhouse ) Denpasar in January to July 2013 a total of six specimens . Cranium of an adult male bull that used a total of six specimens . Five specimens were collected from the National Park consisting Baluran ( two cranium that is banteng baluran 4 and 5 in 1992 ) and the ( three cranium is banteng baluran1, banteng baluran 2 , and banteng baluran 3 in the 2012-2013 ) . Cranium specimen obtained from the sixth bull collection Merubetiri National Park . Measuring instruments used in this study is the large calipers with a precision level of 0.01 cm . The results of this study showed that the cranium cluster of Bali cattle with coefficient range of 0.016 to 0.042 in contrast to the first cluster is bull cranium specimen glaze 1 bull , bull glaze 2 , 3 baluran bull , and the bull merubetiri 1 , with a coefficient value of 0.047 to 0.161 range . Bali cattle cranium differ significantly with cluster cranium second bull is a bull glaze glaze glaze bull 4 and 5 with the coefficient of 0.162 to 0.251 range. Merubetiri bull glaze and form two clusters , the first cluster bull bull glaze consisting of 1 , 2 bull glaze , glaze bull bull merubetiri 3 and 1 , while the second consists of a cluster bull bull bull glaze glaze 4 and 5 . Turns bull glaze specimens collected in 1992 in contrast to the bull glaze specimens collected in 2012 and 2013. Bull glaze specimens collected in 2012 and 2013, closer to the bull merubetiri specimens . And the bull specimens closer distance coefficient with Bali cattle. The conclusion of this study is the overall specimen Bali cattle entered in a cluster , different from the first cluster and cluster bull bull both . The first cluster by cluster bull bull both are in different clusters. Keywords: cluster analysis , relationship , craniometric , bali cattle and banteng.

Perilaku Bermain Anak Sapi Peranakan Ongole (PO) di Blok Merak, Kawasan Resort Labuhan Merak Taman Nasional Baluran Sohih, Ahmad Mauludin; Wiyono, Hidayat Teguh; Mahriani, M.
BERKALA SAINSTEK Vol 6 No 2 (2018)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v6i2.9302

Perilaku bermain merupakan perilaku yang dilakukan hewan dengan tujuan untuk adaptasi, yaitu sebagai latihan dalam menghadapi predator, latihan dalam menyerang, bertarung, seperti melarikan diri atau menyerang lawannya. Perilaku bermain dapat menjadi indikator kesejahteraan hewan (animal welfare). Penelitian ini dilakukan di Blok Merak, kawasan Resort Labuhan Merak Taman Nasional Baluran. Penelitian ini menggunakan lima anak sapi peranakan ongole (PO). Prosedur penelitian ini yaitu; penentuan sampel anak sapi, pelabelan sampel anak sapi, pengamatan perilaku, dan pengukuran kondisi lingkungan. Metode yang digunakan dalam pengamatan perilaku adalah Focal Animal Sampling. Parameter yang diukur yaitu frekuensi dan durasi perilaku bermain anak sapi PO yang meliputi jumping, bucking, running, mock fighting/butting calf, dan mounting. Data hasil pengamatan perilaku bermain anak sapi PO dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa frekuensi dan durasi perilaku bermain yang meliputi jumping dan bucking tiga kali tiga detik, running 27 kali 71 detik, mock fighting/butting calf 45 kali 434 detik, dan mounting yaitu 88 kali 224 detik. Frekuensi dan durasi masing-masing anak sapi PO yaitu anak sapi A 44 kali 215 detik, anak sapi B 24 kali 115 detik, anak sapi C 42 kali 202 detik, anak sapi D 30 kali 83 detik dan anak sapi E 22 kali 120 detik. Kesimpulan penelitian ini adalah ditemukan lima perilaku bermain yang diamati pada anak sapi peranakan ongole (PO) di Blok Merak, kawasan Resort Labuhan Merak yang digembalakan secara liar di savana Taman Nasional Baluran. Kata Kunci: Perilaku Bermain, Anak sapi Peranakan Ongole (PO), dan Taman Nasional Baluran.

PRODUKSI GUM ARABIC BALURAN SEBAGAI PENDUKUNG PENGEMBANGAN WISATA KAMPUNG BANTENG DI KARANG TEKOK SEBAGAI WILAYAH PENYANGGA TN BALURAN Kusumah, Maulana S.; Wiyono, Hidayat Teguh; Subekti, Agus; Muzakhar, Kahar; Winarsa, Rudju
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 4 No 1 (2020): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Forum Dosen Indonesia JATIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v4i1.272

This article is the result of PPDM (Mitra Desa Service Program) about Baluran bos javanicus (Banteng) village tourism. Development of bull village tourism is an effort to solve the problem of wild grazing in Bunaken National Park. In the event of Banteng Village Tourism, it is necessary to support tourism, namely creative industries, agro-tourism, and NTFP production (non-timber forest products). One of the NTFPs that is relied upon is Arabic gum. Currently, cattle breeders in the banteng village area have been able to produce Arabic gum as a result of the introduction of tapping technology by the 2019 PPDM team. The dedication method is in the form of dissemination and field practice. Three groups representing breeders were trained to tap acacia gum through a drilling method combined with ethephon induction as GIS. One week after application, the group begins harvesting gum and submits the results to the group leader. Then the group leader sends the results to the Cooperative in Pondok Pesantren Assalam, Sumberanyar, Banyuputih Situbondo. The amount of Baluran Arabic gum that was collected by the group for three months reached 143.9 kg. This service activity concludes that the strength in producing Baluran Arabic gum is significant in improving the welfare of breeders in supporting the maintenance and retention of a Banteng.

The Diversity of Potential Malaria and Dengue Mosquito Vector from Bangsring Village Wongsorejo District Banyuwangi East Java Azkiyah, Siti Fat'hiyatul; Senjarini, Kartika; Oktarianti, Rike; Wiyono, Hidayat Teguh; Wathon, Syubanul
Jurnal ILMU DASAR Vol 22 No 1 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i1.13601

Bangsring village in Wongsorejo regency has been reported as malaria endemic area in Banyuwangi since 2011. Understanding the diversity and behavior of mosquito vector in this area will be very impotant in developing vector control program. The masquitoes were collected by landing collection outside and inside house by human bites, we also observed the area around cattle cage. During observation periods, a total 633 masquitos were collected consist of 44% malaria potential vector (Anopheles spp.) 19% Dengue fever (DF) potential vector (Aedes spp.) and 36% were not belong to both of them. Out of total collected Anopheles spp. mosquitoes about 65% were indentified as An. vagus, 25% were An. indevinitus, 8% were An. vagus (limosus), and only 2% as well as 0,04 % were An. supictus and An. kochi. Meanwhile we found 19% of Aedes sp. were Ae. aegypti and 81% were Ae. albopictus. This study showed that the dominan potential malaria’s vector is An. Vagus and the dominan DF vector is Ae. albopictus. Mostly colleted Anopheles sp. were exophagic and zoophilic with the highest activities between 06.00-08.00 pm. This was in contras to Aedes sp. where mosfly found endophilic and it has 2 hightime of activities Aedes sp. between 06.00- 07.00 AM and 05.00-06.00 PM. Keywords: Dengue, Malaria, diversity, Anopheles, Aedes.

FREKUENSI ALEL GOLONGAN DARAH SISTEM A-B-O PADA POPULASI SUKU OSING DESA KEMIREN KABUPATEN BANYUWANGI Amania, Novita; Wiyono, Hidayat Teguh; Oktarianti, Rike
BIOMA Vol 5, No 1 (2020): BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i1.3680

Suku Osing di desa Kemiren, Banyuwangi memiliki tradisi perkawinan upek-upekkan, yaitu perkawinan antar kerabat. Perkawinan ini termasuk dalam perkawinan endogami yang menyebabkan menurunnya variasi genetik. Penelitian ini bertujuan untuk mengetahui proporsi fenotip, frekuensi alel, dan kesetimbangan genetic Hardy-Weinberg golongan darah ABO pada populasi suku Osing di Desa Kemiren. Pengambilan sampel dilakukan secara acak. Identifikasi golongan darah dilakukan dengan metode slide test. Analisis penelitian menggunakan uji Chi-square. Hasil penelitian menunjukkan golongan darah pada populasi suku Osing di Desa Kemiren sebagai berikut: golongan darah O (45,95%), A (23,99%), B (21,97%), dan AB (8,09%). Frekuensi alel masing-masing adalah alel IA (0,16), frekuensi alel IB (0,16), dan frekuensi alel i (0,68). Hasil pengujian kesetimbangan genetik Hardy-Weinberg dengan uji Chi-square menunjukkan tidak ada penyimpangan yang signifikan.

Effect of Baluran Gum Arabic on Blood Glucose Level in Diabetic Rat (Rattus Novergicus ) Wiyono, Hidayat Teguh; Utami, Eva Tyas; Wardhani, Dinna Wahyu Putri
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.22533

Diabetes mellitus is a metabolic disease which indicated with increasing blood glucose level. Carbohidrate, protein, mineral and secondary metabolite (alkaloid, tannin and saponin) can be benefical to treat diabetes mellitus. Rats were randomly divided into three groups. First, control group. Second, STZ group, untreated diabetic. Third, STZ+GA 15% group, diabetic treated with GA 15%. Result showed that blood glucose level before STZ induction on control and STZ group within normal range 78,57±11,90 mg/dL and 74,85±6,86 mg/dL. Blood glucose level significantly increase after STZ induction on STZ and STZ+GA 15% group become 375±6,53 mg/dL and 346,42±50,23 mg/dL. Diabetic rat treated with GA 15% revealed decrease in blood glucose level compared to untreated diabetic rat. Blood glucose level on STZ+GA 15% group continuously decrease become 96,42±13,45 mg/dL and 82,14±9,19 mg/dL. In conclusion, GA 15% could reduce blood glucose level on diabetic rat.

Distribusi dan Frekuensi Alel Golongan Darah Sistem ABO dan Rhesus pada Penduduk Pulau Gili Ketapang Probolinggo Oktarianti, Rike; Rezki, Kirana Eka; Wiyono, Hidayat Teguh; Purwatiningsih, Purwatiningsih
BIOSAINTROPIS (BIOSCIENCE-TROPIC) Vol 7 No 1 (2021): Agustus 2021
Publisher : FMIPA - ISLAMIC UNIVERSITY OF MALANG (UNISMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v7i1.452

Sistem penggolongan darah pada manusia yang banyak dikenal adalah sistem ABO dan rhesus. Tujuan penelitian ini adalah Mengetahui distribusi golongan darah sistem ABO dan rhesus serta frekuensi alel pada populasi penduduk pulau Gili Ketapang, kabupaten Probolinggo. Pengambilan sampel dilakukan secara acak dengan cara dilakukan pengundian jumlah sampel yang diambil sebanyak 384 jiwa. Identifikasi golongan darah sistem ABO pada penelitian ini dilakukan dengan metode slide dengan prinsip antigen (aglutinogen) yang direaksikan dengan antibodi (aglutinin). Hasil penelitian menunjukkan distribusi golongan darah sistem ABO dan Rhesus O+ (46,61%), B+ (24,22%), A+ (23,18%), dan AB+ (5,99%). Frekuensi alel IA(0,16), frekuensi alel IB (0,16), frekuensi alel i (0,68), dan frekuensi alel rhesus positif (Rh+) adalah 1

FREKUENSI ALEL GOLONGAN DARAH SISTEM A-B-O PADA POPULASI SUKU OSING DESA KEMIREN KABUPATEN BANYUWANGI Amania, Novita; Wiyono, Hidayat Teguh; Oktarianti, Rike
BIOMA Vol 5, No 1 (2020): BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i1.3680

Suku Osing di desa Kemiren, Banyuwangi memiliki tradisi perkawinan upek-upekkan, yaitu perkawinan antar kerabat. Perkawinan ini termasuk dalam perkawinan endogami yang menyebabkan menurunnya variasi genetik. Penelitian ini bertujuan untuk mengetahui proporsi fenotip, frekuensi alel, dan kesetimbangan genetic Hardy-Weinberg golongan darah ABO pada populasi suku Osing di Desa Kemiren. Pengambilan sampel dilakukan secara acak. Identifikasi golongan darah dilakukan dengan metode slide test. Analisis penelitian menggunakan uji Chi-square. Hasil penelitian menunjukkan golongan darah pada populasi suku Osing di Desa Kemiren sebagai berikut: golongan darah O (45,95%), A (23,99%), B (21,97%), dan AB (8,09%). Frekuensi alel masing-masing adalah alel IA (0,16), frekuensi alel IB (0,16), dan frekuensi alel i (0,68). Hasil pengujian kesetimbangan genetik Hardy-Weinberg dengan uji Chi-square menunjukkan tidak ada penyimpangan yang signifikan.

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