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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Atma Inovasia
Exsyupransia Mursyanti
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Social Sciences Other

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Mutu Bakteriologis Pindang Tongkol (Euthynnus affinis) yang Berasal dari Beberapa Pasar di Yogyakarta Ekawati Purwijantiningsih; Exsyupransia Mursyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i1.2821

Abstract

Food poisoning cases need to be examinated intensively. There have been five poisoning cases reported between 2003-2004 in Sleman area. Three of them were caused by contaminated tuna fish. The objective of this research was to determine bacteriological quality of salted tuna fish from several markets in Yogyakarta. Salted tuna fish sample were collected from five markets, namely Beringharjo, Condongcatur, Demangan, Kranggan and Sentul. In each market, four vendors were selected. Bacteriological test were done including total microbe cells utilizing PCA medium, Coliform test utilizing VRBA medium. Identification and isolation of Vibrio parahaemolyticus use pre-enrichment selective medium of Alkaline Petone Water and selective Chromogenic medium agar for Vibrio. Salmonella test utilizing Chromogenic agar continued by utilizing TSI and LIA medium. The result showed that bacteriological quality of salted tuna fish from markets in Yogyakarta did not fulfill the requirements based on SNI 01-2717-1992. It was shown that total microbial count were high, i.e. 2.4 x 105 – 5.27 x 107 CFU/ g. Coliform count ranged from < 2.5 x 101 - > 6.5 x 106 CFU/g. From 20 samples that were brought to analyzed, 60% was positively presumed contaminated by V. parahaemolyticus and 50% was positively presumed contaminated by Salmonella. 
Peningkatan Produksi Asam Glutamat Corynebacterium glutamicum dengan Penambahan Penisilin pada Fase Logaritmik Exsyupransia Mursyanti; Sri Lestari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2843

Abstract

One way to increase production and excretion of glutamic acid was to increase cell's permeability. Penicillin has a potency to change the cell permeability by inhibitng cell wall synthesis. However, penicillin treatment was effective only for actively dividing cells. Therefore, such a research was done to study on the time of penicillin treatment to the medium, so that it can be found optimal cell biomass to produce maximum glutamic acid. The cell utilized in the research was Corynebacterium glutamicum IFO 12168 that was in batch cultured. Concentration of penicillin added was 5 unit/ml and treated at incubation periods 12, 14, 16, 18, and 20 hours, respectively, after inoculation. The steps of the research were as follows purification test, growth pattern, and glutamic acid production. Parameters measured at the end of the fermentation were cell biomass, reduced sugar concentration, medium’s pH, and glutamic acid concentration. Data was analysed utilizing Anova and the significant difference between treatments were tested using Duncan’s Multiple Range Test (DMRT). The growh pattern shown that logarithmic phase was reached at 2 to 22 hrs of incubation periods, therefore the treatment of penicillin was given at 12, 14, 16, 18, and 20 hrs of incubation periods. Cell biomass produced was corelate with the concentration of reduced sugar in the medium. Measured pH of the medium at the end of the fermentation was on the pH range for the growth of C. glutanicum. The research concluded that Penicillin treatment was able to increase significantly the glutamic acid production compatred to control treatment. Time accuracy of penicillin treatment to produce maximum glutamic acid (154319,60 µg/ml) was on 18 hrs of incubation period.
Kualitas Minyak Kelapa Hasil Fermentasi Saccharomyces cereviceae Exsyupransia Mursyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2880

Abstract

One aspects of utilization of coconut fruit is its palm oil. Traditionally coconut oil was made by “klentik” method, which was time and energy consuming. The important thing in the production of coconut oil from its xanthan is the separation of protein from oil. One of separation methods is by creating low pH (acid) condition that is near to protein isoelectric point. This research objective was to determine the potency of Saccharomyces cereviceae, that has an optimum growth pH within the range of protein isoelectric point, to produce coconut oil. Three isolates of Saccharomyces were used, i.e. isolate A that was coming from alcohol industry (S. cereviceae strain Kyowa), isolate B that was natural type (isolated from fruits), and isolate C that was S. cereviceae in the form of powder (commercial isolate, Saf-instant). Coconut xanthan used in the research was prepared from old coconut fruits. Research stages including pure culture test of S. cereviceae, starter preparation, xanthan production, fermentation process, oil separation and heating. Quantitative and qualitative analyses were done for oil rendement, water content, free-fatty acid concentration, saponification number, iod number, and organoleptic test (color and aroma). The results showed that the quality of coconut oil produced by fermentation process was better than traditional way in term of rendement value, water content, saponification number, and iod number. This was supported also by organoleptic test that aroma and color of coconut oil from fermentation were more preferred by the panelists. Furthermore, the quality of oil produced by S. cereviceae fulfilled the standart stated by the Indonesian Department of Commerce (SII-1976) except for saponification number.
Eksplorasi Genom, terkuaknya misteri manusia (Kajian Buku) Exsyupransia Mursyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2882

Abstract

Eksplorasi genom manusia yang terwadahi dalam Human Genom Project (HGP) telah berhasil mengidentifikasi keseluruhan genom (DNA) manusia dalam waktu 13 tahun (1990-2003), dua tahun lebih cepat dari yang ditargetkan (15 tahun). Karya yang spektakuler ini memberikan banyak informasi tambahan mengenai genom manusia. Genom manusia yang dahulu diperkirakan berukuran tiga milyar basa, ternyata tersusun atas 2,3 milyar nukleotida yang terdiri dari ±30.000 gen, 50% gen tersebut sudah diketahui fungsinya. Kromosom no.1 mengandung gen paling banyak (2.968 gen) sedangkan kromosom Y mengandung gen paling sedikit (231 gen). Selain itu, diinformasikan pula bahwa pembeda manusia yang satu dengan yang lain terletak pada tiga juta lokasi single nucleotide polymorphisms (SNPs).
Pola Pertumbuhan dan Produksi -Amilase Bacillus amyloliquefaciens pada Substrat Pati Jagung dengan Variasi pH Awal Media dan Waktu Inkubasi Sisilia Sri Wahyuningsih; Exsyupransia Mursyanti; P. Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.2895

Abstract

The aims of this study  were to identify the growth curve of  B. amyloliquefaciens on  corn-starch and non corn starch addition media,  number of cells  and production of a-amylase on variety initial pH during the stationary phase. The growth curve of B. amyloliquefaciens was made using the water optical density on both  medium which has inoculated by microbes. The experimental design for the a-amylase production was factorial completely randomized design (6 x 3 x 3). There were two factors included in this study i.e. initial  pH of the media ( 5,  5.5,  6,  6.5,  7 and 7.5) and incubation times (16, 18 and 20 hours). The results showed that B. amyloliquefaciens growth curve on medium with corn starch was slower than on medium without corn starch. Production of  a-amylase and number of cells were having similar patterns in all treatments, i.e. increased until optimum pH and incubation time were reached. The number of cells and a-amylase production were optimal at pH 6.5 for 18 hours incubation whereas the number of cells  (about 2.8542 x 108 cells/ml)  and a-amylase production (1.4467 units/ml) were optimal at pH 6.5 for 18 hours  incubation.
Potensi Antibakteri Ekstrak Tanaman Suku Rubiaceae dan Aplikasinya dalam Sediaan Hand Sanitizer Sriwahyuni Iskandar Rasid; Exsyupransia Mursyanti; Boy Rahardjo Sidharta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 2 (2021): June 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i2.4165

Abstract

Tanaman suku Rubiaceae merupakan tanaman yang banyak digunakan sebagai obat tradisional. Kandungan senyawa aktif yang ada dalam tanaman suku Rubiaceae meliputi alkaloid, flavonoid, saponin, tanin, fenol, dan minyak atsiri (antrakuinon) yang berpotensi sebagai senyawa antibakteri. Kandungan alkohol dalam hand sanitizer yang beredar di pasaran memberi rasa iritasi dan terbakar pada kulit jika digunakan secara terus menerus sehingga inovasi hand sanitizer dari tanaman suku Rubiaceae dapat dijadikan alternatif untuk membersihkan tangan. Hasil literature review ini menunjukkan bahwa ekstrak dan sediaan hand sanitizer tanaman suku Rubiaceae yang diuji aktivitas antimikrobianya dengan metode difusi agar dan broth dilution mempunyai daya antibakteri terhadap S. aureus dan E.coli. Sediaan hand sanitizer tanaman suku Rubiaceae juga memiliki kualitas karakteristik fisik seperti bentuk, warna, dan bau serta  stabilitas seperti pH, viskositas, homogenitas, daya lekat dan daya sebar yang baik. Kategori kualitas baik ini didasarkan pada kesesuaian SNI terkait kestabilan gel antiseptik.
Pelatihan dan Pendampingan Prapanen, Panen dan Pascapanen kepada Petani Jahe Area Paroki Boro, Kabupaten Kulon Progo sendy junedi; Ines Septi Arsiningtyas; P Kianto Atmodjo; Exsyupransia Mursyanti; Boy Rahardjo Sidharta; Stefani Santi Widhiastuti
Jurnal Atma Inovasia Vol. 3 No. 5 (2023): September
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v3i5.7594

Abstract

Paroki Santa Theresia Lisieux (STL) Boro terletak pada area pengembangan pariwisata sehingga berpotensi menjadi desa wisata dengan amenitas khas berupa jahe emprit. Produksi jahe pada area ini memiliki keterbatasan kuantitas, kontinuitas, dan kualitas karena minimnya pengetahuan dan ketrampilan petani dalam penanaman dan pemeliharaan tanaman serta pengolahan rimpang jahe menjadi produk jadi. Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta memberikan pelatihan dan pendampingan untuk memperbaiki pengetahuan dan ketrampilan petani jahe di area Paroki STL Boro. Pelatihan dan pendampingan dibagi menjadi tujuh kali pertemuan yang terdiri dari sosialisasi dan evaluasi pengetahuan dalam bentuk kuesioner, dilanjutkan dengan pelatihan tentang prapanen, panen, pascapanen primer dan sekunder, serta yang terakhir adalah evaluasi berupa diskusi dan kuesioner. Topik pertemuan pascapanen sekunder terdiri dari pembuatan produk kesehatan (serbuk jahe instan) dan produk kosmetik (lulur jahe). Hasil analisis data kuesioner secara semikuantitatif menunjukkan terjadi peningkatan pengetahuan petani sebesar 75% antara sebelum dan sesudah pelatihan. Dengan adanya pelatihan, petani di Paroki STL Boro mampu menghasilkan produk jahe instan yang telah dipasarkan secara terbatas.
Co-Authors Boy Rahardjo Sidharta Ekawati Purwijantiningsih Ines Septi Arsiningtyas P Kianto Atmodjo P. Kianto Atmodjo sendy junedi Sisilia Sri Wahyuningsih SRI LESTARI Sriwahyuni Iskandar Rasid Stefani Santi Widhiastuti