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Ratna Yuliani
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Journal : Biomedika

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AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA Dr. Muhtadi , MSi.; Ria Ambarwati; Ratna Yuliani
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i2.252

Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .
EFEK ANTIINFLAMASI INFUSA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg) Roscoe) PADA TIKUS YANG DIINDUKSI KARAGENIN Tanti Azizah Sujono; Raudatul Patimah; Ratna Yuliani
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i2.253

Abstract

In-flammation is the body’s reaction to foreign substances that enter the body with signs of redness, heat, swelling, pain, and impaired organ functions. Curcuma rhizome including medicinal plants that have properties relieve the pain and inflammation of the skin. The purpose of this research was to examine the anti-iflammation effect of Curcuma zedoaria (Berg) Roscoe Rhizome infusion on carrageenan induced paw edema in rats. This research used experimental method with completely randomized design. Twenty five healthy Wistar male rats, 2-3 months old and 150-250 g divided into five groups and each group consist of 5 rats. Rats in group I that served as negative control were given 2.5 mL/200gbw of aquadest. Rats in group II as positive control were given sodium diclofenac with dose of 6.75 mg/kgbw. Group III, IV, and V were given Curcuma zedoaria (Berg) Roscoe Rhizome infusion with dose of 0.625, 1.250 and 2.500 g/kgbw, respectively. Treatments were given per oral 1 hour before injection of 0.1 ml carrageenan 1% subplantar. Rat paw edema volume was measured before and after carrageenan injection. The measurement was done every 0.5 hour for 6 hours observation. Area Under the Curve (AUC) that is calculated from paw edema volume data, was use to calculate percentage of anti-inflammatory effect. Data were analyzed with one way Anova and Least Signifi cant Difference with confi dence level 95%. Infusion of Curcuma zedoaria Rhizome with doses of 0,625, 1,250, and 2,500 g/kgbw had anti-inflammatory effect in Wistar male rats which were induced by Carrageenan 1%. Percentage of anti-inflammation effect (44,16+5,11)%, (48,70+7,05)%, (59,09+9,61)% respectively and the effects were equivalent with positive control.Key words: Anti-inflammation, Curcuma zedoaria (Berg) Roscoe, infusion, Carrageenan.
Co-Authors Anita Sukmawati Dr. Muhtadi , MSi. Muhammad Nur Prasetyo NUR AINI Raudatul Patimah Ria Ambarwati Sternatami Liberitera Tanti Azizah Sujono