Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal PROSIDING SEMINAR KIMIA
Lila Karina Nuryanti
Jurusan KimiaFakultas Matematika dan Ilmu Pengetahuan AlamUniversitas Mulawarman
Chemistry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM AMILASE PADA UMBUT KELAPA (Coccos nucifera L.) Lila Karina Nuryanti; Winni Astuti; Rudi Kartika
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Amylase is an enzyme that can catalyze stach hydrlysis into its simplier molecule as glucose. Coconut’s buds (Coccos nucifera L.) have high enough of carbohydrate content which amylase could have existed. The research are to determine protein concentration, optimum catalyst function and specified activity of amylase in coconut’s buds (Coccos nucifera L.). Coconut’s buds (Coccos nucifera L.) are isolated by extraction method using phospate buffer solution 0,1 M pH 7. The determination of protein concentration are used Bradford method. The determination of optimum catalyst function are used dinitrosalicylate acid (DNS), optimum catalyst fuction which are determined include of optimum pH, optimum temperature and optimum substrate concentration. The determination of specified activity of amylase are tested in optimum condition. The result of research are obtained the protein content are 2,933 mg/mL. The specified activity which are obtained from coconut’s buds (Coccos nucifera L.) are 80,376 U/mg in pH 6, temperature 50 ºC and substrate concentration 6,5 mg/mL Keywords : amylase, coconut’s buds, dinitrosalicylate acid (DNS) and specified activity.
Co-Authors Rudi Kartika, Rudi Winni Astuti