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All Journal PROSIDING SEMINAR KIMIA Jurnal Kimia Mulawarman JURNAL ATOMIK
Winni Astuti
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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PENGARUH ION LOGAM TERHADAP AKTIVITAS EKSTRAK KASAR LIPASE DARI KECAMBAH BIJI CEMPEDAK (Artocarpus integer (Thunb.) Merr.) Delina Khairunnisa; Winni Astuti; Rudi Kartika
PROSIDING SEMINAR KIMIA 2021: SEMINAR NASIONAL KIMIA 2021
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Kecambah biji tanaman Cempedak (Artocarpus integer (Thunb.) Merr.) memiliki aktivitas lipase yang dapat dimanfaatkan untuk meningkatkan nilai ekonomis tanaman tersebut. Penelitian ini bertujuan untuk mengisolasi lipase dari kecambah biji cempedak dan mengetahui pengaruh ion logam terhadap aktivitas ekstrak kasar lipase. Penentuan pengaruh ion logam terhadap aktivitas lipase dilakukan dengan variasi jenis ion logam, yaitu Na+, K+, Ba2+, Mg2+ dan Fe3+dengan konsentrasi 0,1 M dan diinkubasi selama 120 menit. Aktivitas enzim lipase ditentukan terhadap substrat minyak zaitun dengan metode titrimetric pada pH serta suhu optimal. Hasil penelitian menunjukkan jenis ion logam berpengaruh terhadap aktivitas enzim lipase dari ekstrak kasar kecambah biji cempedak. Pada penambahan ion Fe3+, Na+ dan Ba2+ aktivitas menurun menjadi berturut-turut sebesar 57,98%; 63,03% dan 93,28% sedangkan pada penambahan ion Mg2+ dan K+ aktivitas enzim lipase meningkat menjadi berturut-turut sebesar 131,09% dan 101,68%. Kata kunci: Kecambah biji cempedak (Artocarpus integer (Thunb.) Merr.), Lipase, Ion logam
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM LIPASE DARI PERKECAMBAHAN BIJI DURIAN (Durio zibethinus L) Dzulkarnain Dzulkarnain; Winni Astuti; Saibun Sitorus
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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This research was conducted to isolate and to know the optimum lipase working conditions (pH, temperature and concentration of substrate), and to know lipase activity from germination of durian seed (Durio zibethinus L.). Isolation is done by 2 stages namely, homogenization and centrifugation. Centrifugation is done at a speed of 12000 rpm with a temperature of 4°C for 30 minutes. Activity by using titration method and protein concentration using method. The optimum lipase temperature obtained was 60°C, optimum pH was 7, and the optimum substrat concentration was 1 % v/v. The lipase specific activity yielded 0,221 U/mg. Keywords: lipase, Durio zibethinus L., Lipase specific activity.
PEMBUATAN ETANOL DARI UMBI GARUT (Maranta arundinacea) SECARA HIDROLISIS ENZIMATIS DENGAN PENAMBAHAN NPK SEBAGAI NUTRISI FERMENTASI MENGGUNAKAN Saccharomyces cerevisiae Wulan Verani; Rudi Kartika; Winni Astuti
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Ethanol production as a biofuels successor fossil fuels from arrowroot tubers (Maranta arundinacea) through enzymatic hydrolysis process and fermentation use Saccharomyces cerevisiae has been carried out. This research aimed to determine levels of ethanol production by various concentration of nutrients added and variations time of fermentation. Hydrolysis process by enzymatic through a liquefaction phase with α-amylase and gluco-amylase at the saccharification stage. In the fermentation process NPK is used as a fermentation nutrient. The variations of NPK used is 1%, 2% and 3% (w/v) and the variations of fermentation time is 5, 7 and 9 days. The highest ethanol concentration of ethanol obtained are in addition of NPK nutrition at 2% (w/v) for 7 days, with the obtained ethanol concentration of 46, 989%. Keywords: ethanol, arrowroot tubers (Maranta arundinacea), NPK, fermentation.
UJI TOKSISITAS DENGAN METODE BSLT EKSTRAK KASAR KULIT BATANG TAMPOI (Baccaurea macrocarpa) Diasttri Wahyu Rahmah Day; Erwin Erwin; Winni Astuti
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Baccaurea macrocarpa (Tampoi) merupakan genus Baccaurea termasuk tumbuhan yang berbuah. Secara tradisioanal daun dan kulit batangnya dipergunakan sebagai bahan obat. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas dari ekstrak kulit batang Tampoi. Metode yang digunakan meliputi ekstraksi dan partisi, uji fitokimia, Brine Shrimp Lethality Test (BSLT) ekstrak dan fraksi-fraksinya. Hasil uji fitokimia terhadap ekstrak kasar alkaloid, triterpen, fenol and flavonoid. Hasil uji toksisitas ekstrak kasar termasuk katagori tidak beracun dengan nilai LC50 19364,71 ppm. Keywords : Baccaurea macrocarpa, tradisional, Toksisitas, BSLT.
ISOLASI AMILASE DARI BIJI MANGGA (Mangifera indica L.) Eno Julia Shafarini; Winni Astuti; Rudi Kartika
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2017
Publisher : PROSIDING SEMINAR KIMIA

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Penelitian ini bertujuan untuk mengisolasi amilase dari biji mangga (Mangifera indica L.), mengetahui kadar protein, mengetahui kondisi optimum (derajat keasaman, suhu dan konsentrasi substrat), serta aktivitas spesifik amilasenya. Penentuan kadar protein total dilakukan dengan menggunakan metode Bradford. Penentuan kondisi optimum dan aktivitas spesifik amilasenya dilakukan dengan menggunakan metode asam dinitrosalisilat (DNS). Hasil penelitian ini menunjukkan kadar protein total yang terdapat dalam ekstrak kasar enzim biji mangga (Mangifera indica L.) adalah 3.176,9 μg/mL, aktivitas amilase sebesar 0,068 U/mL dan aktivitas spesifiknya sebesar 0,858 U/mg. Kata Kunci: Kecambah Biji Nangka (Artocarpus heterophyllus Lam), Ekstrak Kasar Enzim, Protein Total dan Amilase.
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM AMILASE DARI REBUNG BAMBU SERIT (Gigantochloa robusta Kurz.) Fajarwati Siska; Winni Astuti
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Amilase merupakan enzim yang dapat menghidrolisis pati. Amilase dapat berasal dari tanaman, mikroorganisme dan manusia. Rebung merupakan salah satu sumber amilase yang dipilih karena mengandung glukosa. Penelitian ini dilakukan untuk mengisolasi, mengetahui kadar protein, mengetahui kondisi kerja optimum (pH, suhu dan konsentrasi substrat), serta aktivitas spesifik amilase yang berasal dari rebung bambu serit (Gigantochloa robusta Kurz.). Penentuan konsentrasi protein dilakukan dengan menggunakan metode Bradford. Penentuan kondisi kerja optimum dan aktivitas spesifik dilakukan dengan menggunakan metode asam dinitrosalisilat (DNS). Hasil penelitian ini menunjukkan bahwa kadar protein yang terkadung dalam ekstrak kasar enzim dari rebung bambu serit adalah 2,83 mg/mL. Kondisi kerja optimum amilase dari rebung bambu serit adalah derajat keasaman (pH) 6, suhu 45ºC dan konsentrasi substrat 6,5 mg/mL. Aktivitas spesifik amilase yang dihasilkan dari rebung bambu serit adalah 236,339 U/mg. Kata kunci: amilase, rebung bambu, asam dinitrosalisilat (DNS) dan aktivitas spesifik.
PENAPISAN BAKTERI TERMOFILIK PENGHASIL AMILASE DARI SUMBER AIR PANAS DONDANG DI KECAMATAN MUARA JAWA Muhammad Taufik A. A. R; Winni Astuti; Erwin Erwin
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2017
Publisher : PROSIDING SEMINAR KIMIA

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Amylase producing bacteria have been isolated from Dondang hot springs in Muara Jawa sub-district based on clear zone. Dondang hot springs became the site of the discovery of thermostable bacteria. Obtained 1 selected isolate showed amylase activity with the formation of clear zone around the colony on solid media containing 1% starch. Based on the morphology and physiology tests the selected isolates showed the genus Bacillus sp. The results showed total protein concentration of crude extract of enzyme from amylase producing bacteria isolate that is 2, 4068 μg /mL. Keywords: Thermophilic bacteria, amylase, clear zone, protein concentration.
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM AMILASE DARI TALAS BOGOR (Colocasia esculenta (L.) shoot) Rezky Estrada; Rudi Kartika; Winni Astuti
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Isolation and determination of optimum amylase work condition has been conducted from Bogor taro (Colocasia esculenta (L.) shoot). The aim of this research is to understand the optimum work condition and specific activity of amylase from Bogor taro. Amylase was isolated using homogenization techniques and sentrifugation. The raw extract of enzyme that contain amylase produced by Bogor taro then used to determine the total protein concentration and tested its activity in pH condition, temperature and maximum substrate concentration in various ways. The determination of total protein concentration was done with Bradford method, while the optimum work condition and specifik activity of amylase was done with dinitrosalycylic acid (DNS) method. The result of this reseach show the total protein concentration in Bogor taro is 7,88 μg/mL. Optimum work condition in Bogor taro is on acidity level (pH) 6, 600C temperature and 6 mg/mL substrate concentration. Specific activity from Bogor taro is 9573,68 U/mL. Keywords: amylase, taro tuber Bogor (Colocasia esculenta (L.) shoot), specific activity of amylase.
ISOLASI DAN PENENTUAN KONDISI KERJA OPTIMUM AMILASE PADA UMBUT KELAPA (Coccos nucifera L.) Lila Karina Nuryanti; Winni Astuti; Rudi Kartika
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2018
Publisher : PROSIDING SEMINAR KIMIA

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Amylase is an enzyme that can catalyze stach hydrlysis into its simplier molecule as glucose. Coconut’s buds (Coccos nucifera L.) have high enough of carbohydrate content which amylase could have existed. The research are to determine protein concentration, optimum catalyst function and specified activity of amylase in coconut’s buds (Coccos nucifera L.). Coconut’s buds (Coccos nucifera L.) are isolated by extraction method using phospate buffer solution 0,1 M pH 7. The determination of protein concentration are used Bradford method. The determination of optimum catalyst function are used dinitrosalicylate acid (DNS), optimum catalyst fuction which are determined include of optimum pH, optimum temperature and optimum substrate concentration. The determination of specified activity of amylase are tested in optimum condition. The result of research are obtained the protein content are 2,933 mg/mL. The specified activity which are obtained from coconut’s buds (Coccos nucifera L.) are 80,376 U/mg in pH 6, temperature 50 ºC and substrate concentration 6,5 mg/mL Keywords : amylase, coconut’s buds, dinitrosalicylate acid (DNS) and specified activity.
AKTIVITAS AMILASE, PROTEASE dan LIPASE DARI MADU LEBAH Trigona sp, Apis mellifera dan Apis dorsata Hendric Surya Putra; Winni Astuti; Rudi Kartika
JURNAL KIMIA MULAWARMAN Vol 16 No 1 (2018)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v16i1.433

Abstract

Activities of amylase, protease and lipase from honey Trigona sp, Apis mellifera and Apis dorsata, determination protein concentration and the activity protease done with Bradford method, the determination of the glucose standard and activity amylase done with 3,5-dinitro salicylic acid (DNS) method and activity lipase done with acid-base titration with coconut oil substrate. The honey from Trigona sp has value of the activity amylase and lipase respectively by 0,0136 U / mg and 0,359 U/mg, whereas honey Apis mellifera has activity of protease, amylase and lipase of each 1,22 x 10-6 U/mg; 0,944 U/mg and 0,304 U/mg and then honey Apis dorsata has amylase and lipase activity of each of 0,0645 U/mg and 0,287 U/mg.
Co-Authors Ade Rezki Purnamasari Agnes Lebang Chairul Saleh Dea Rakasiwi Delina Khairunnisa Diasttri Wahyu Rahmah Day Djihan Ryn Pratiwi Dzulkarnain Dzulkarnain Elfiza Amalia Eno Julia Shafarini Erwin Erwin Eva Marliana, Eva Fajarwati Siska Hendric Surya Putra Hendrica Mini Vera Irvan Afandi Lila Karina Nuryanti Muhammad Taufik A. A. R mutma Inna Inna Nanda Arwidhiah Noor Hindryawati Rahmat Gunawan Resy Lelita Rezky Estrada Rudi Kartika, Rudi Safridah Hannum Nasution Saibun Sitorus Siti Mar puah Wulan Verani