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All Journal HAYATI Journal of Biosciences Jurnal Kimia Terapan Indonesia

OEI BAN LIANG

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Author-ID : 306997

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology

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Articles

Characterization of Extracellular Penicilin G Acylase Produced by A New Local Strain of Bacillus subtilis BAC4 SUPARTONO SUPARTONO; ENNY RATNANINGSIH; SADIJAH ACHMAD; OEI BAN LIANG
HAYATI Journal of Biosciences Vol. 15 No. 2 (2008): June 2008
Publisher : Bogor Agricultural University, Indonesia

Penicillin G acylase (PGA) which catalyses penicillin G hydrolysis reaction is a key enzyme for the industrial production of penicilin G derivatives used in therapeutics. A new local strain of Bacillus subtilis BAC4 was found capable of producing extracellular PGA. However, characteristics of this extracellular PGA are not known. The goal of this research was to characterize the extracellular PGA produced by B. subtilis BAC4. Enzyme production was carried out by batch fermentation, followed by enzyme purification and characterization of the PGA. The PGA activity was determined by the Kornfeld method, with optimal activity for hydrolysing penicillin G observed at 43 oC and pH 8.5. The activation energy of penicillin G hydrolysis by the PGA of B. subtilis BAC4 was determined as 4.9 kcal.mol-1 and Vmax and Km values were found to be 0.7 Ã‚Âµmole.min-1.mg-1 and 3.5 mM respectively. PGA catalytic activity was competitively inhibited by phenylacetic acid with an inhibition constant, Ki(PAA) , of 347.2 mM. It was concluded that the extracellular PGA of B. subtilis BAC4 can hydrolyse penicillin G efficiently. Key words: PGA, extracellular, Bacillus, local

FERMENTASI ASAM SITRAT DARI TETES TEBU, SECARA BIAK-RENDAM DENGAN Aspergillus niger Milono Poesponegoro; Oei Ban Liang
Jurnal Kimia Terapan Indonesia Vol 1, No 2 (1991)
Publisher : Research Center for Chemistry - LIPI

A study to determine the optimum condition of !ubmerged. citric acid fermentatlon of calle molasses with Aspergillus niger has been conducted. Effects of the strains of Aspergillus niger, initial concentration of total reducing sugars and initial pH of the medium 011 citric accumulation were investigated. One-stage submerged culture fermentation process was curried out in a 300 ml Erlenmeyer flask which contained 50 ml liquid medium at 30°C, in an orbital shaker incubator operated at 200 rpm. While a two-stage sub-merged [ermen tation process was performed at30Ve in a stirred fermentor containing 2.5 liters of liquid medium at a constant pH, with 1 Will aera, tion and agitation at a speed of 700 rpm. The [ermeutation process was followed by monitoring the changes ill pH values, concentrations of total reducing sugars, dry weight of cellular biomass, and citric acid concentration in the culture medium, during 7 days of [ermentation time. It was obtained that strain of the 1II0uld, concentration of total reducing sugars as well as pH of the medium affected the submerged citric acid fermentation process with Aspergillus niger. Aspergillus niger ATCC 11414 was found to be the best strain aI/lOng the eight strains tested. High initial concentrations of total reducing sugars (15 - 20%) and low initial pH of the medium (less than 3.0) were the optimum conditions for citric acid accumulation by Aspergillus niger ATCC 11414.

EFFECTS OF TRACE METALS AND MEDIUM COMPOSITION ON THE GROWTH OF Aspergillus niger ATCC 11414, IN A SUBMERGED CULTURE Milono Poesponegoro; Oei Ban Liang
Jurnal Kimia Terapan Indonesia Vol 3, No 2 (1993)
Publisher : Research Center for Chemistry - LIPI

In an attempt to optimize citric acid fermentation, a study has been conducted to determine optimum nutritional conditions for the growth of Aspergillus niger ATCC 11414. The objective of the study was to obtain information on the growth of this strain in a submerged culture for the study of citric acid production The following article summarizes the results of study on the effects of trace metals and composition of chemically defined medium on mycelial growth of A.niger ATCC 11414. Chemically defined media containing glucose as the carbon source and energy were used throughout the work. Growth experiments were carried. Out by a submerged culture process, in a 300-ml Erlenmeyer flask which contained 50ml liquid medium. The process was conducted at 30°C for 4 days in an orbital shaker incubator operated at 200 rpm. The cultivation process was followed by monitoring the changes in the culture medium of the concentrations of biomass, total reducing sugars, citric acid, and pH of the medium. It was concluded that copper (II), iron (II), zinc (II), and manganese (II) ions had a remarkable effect on the growth of A. niger ATCC 11414. With 5% glucose, the study showed that 5 - 15 ppm copper, 0.5 - 25 ppm iron and 0.5 - 25 ppb manganese ions were optimal for the growth of the strain. The growth of the strain increased with the increase of Zn2+ added (0.5 - 25 ppm). The most optimal medium for the growth of A.niger ATCC 11414 was found to be able to produce more than 16 g of dry weight of biomass for 50 g glucose.

PENGARUH LIMITASI NUTRIEN PADA FERMENTASI ASAM SITRAT BIAK-RENDAM,SECARA 2-TAHAP DENGAN Aspergillus niger ATCC 11414 Milono Poesponegoro; Oei Ban Liang
Jurnal Kimia Terapan Indonesia Vol 1, No 2 (1991)
Publisher : Research Center for Chemistry - LIPI

The aim of the study was to determine the type and level of nutrient limitation for stimulation of citric accumulation by Aspergillus niger ATCC 11414. The study focused all the effects of substrate concentration, nutrient limitation and the concentration of pre-cultred mycelium on citric acid accumulation. Citric acid fermentailon was carried out by a 2-stage process, where the growth stage and citric acid production stage were done separately, either using shake-flask culture or stirred fermentcr method. The fermentation process was followed by monitoring the changes in the pH value and in the concentrations of total reducing sugars, cellular biomass, and citric acid in the culture medium. Results of the study showed that nutrient limitation inhibited the growth of mould and could be all important factor for stimulation of citric acid accumulation by Aspergillus niger ATCC 11414. Phosphorous limitation was found to be the most effective than the limitation of other nutrients tested, for stimulation of citric acid accumulation. The results also revealed that there was a relationship between phosphorous limitation, mycelium concentration and the efficiency of citric acid production by Aspergillus niger ATCC 11414.

Co-Authors Enny Ratnaningsih Milono Poesponegoro Sadijah Achmad Supartono -

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