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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Jurnal Matematika & Sains Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Enny Ratnaningsih
Departemen Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology

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Purifikasi Protein Fusi MBP-Mga Streptococcus pyogenes Hasil Ekspresi Heterolog di Escherichia coli Muhaimin Muhaimin; Oei Ban Liang; Enny Ratnaningsih; Endang Purwantini; Debbie Sofie Retnoningrum
Jurnal Matematika & Sains Vol 10, No 1 (2005)
Publisher : Institut Teknologi Bandung

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Abstract

Optimization of purification process of Streptococcus pyogenes’s MBP-Mga fusion protein from Escherichia coli pMALMga carrying mga49 gene had been carried out. The purification was conducted by using affinity chromatography column with amylose ligands. SDS-PAGE analysis gave a band at 104 kDa showing a pure MBP-Mga fusion protein.
Optimasi Proses Overproduksi, Pemurnian dan Karakterisasi Protein Mga Sebagai Molekul Target Untuk Pencegahan Infeksi oleh Streptococcus Pyogenes Muhaimin Muhaimin; Oei Ban Liang; Enny Ratnaningsih; Endang Purwantini; Debbie Soefie Retnoningrum
Jurnal Matematika & Sains Vol 8, No 3 (2003)
Publisher : Institut Teknologi Bandung

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Abstract

Optimization of overproduction, purification and characterization of Streptococcus pyogenes’s Mga recombinant protein from Escherichia coli pMALMga carrying mga49 gene had been carried out. The overproduction optimization was conducted by culturing the Escherichia coli pMALMga of OD600 = 0,7 in LB medium with or without glucose, and induced by 0,3 mM IPTG for a certain period of time. The protein produced was MBP-Mga fusion protein with a molecular weight of about 104 kDa. The purification was conducted by affinity chromatography using amylose ligands at pH 7,4 and continued by SDS-PAGE. One band identified at 104 kDa showed that the MBP-Mga fusion protein was pure enough for further analysis. The MBP-Mga protein was then injected into rabbit to produce polyclonal antibodies. The antibody development was monitored by dot blot and Western blot. The antibodies produced gave a positive reaction to MBP-Mga fusion protein. Adsorption and precipitation of the polyclonal antibodies which gave a positive reaction to MBP protein was done by adding pure MBP protein (56 kDa) produced by Escherichia coli pMALc2, and purified by affinity chromatography using amylose ligands at pH 7,4. The results showed that the antibodies gave a positive reaction to Mga recombinant protein and a negative reaction to MBP recombinant protein.
Characterization of Extracellular Penicilin G Acylase Produced by A New Local Strain of Bacillus subtilis BAC4 SUPARTONO SUPARTONO; ENNY RATNANINGSIH; SADIJAH ACHMAD; OEI BAN LIANG
HAYATI Journal of Biosciences Vol. 15 No. 2 (2008): June 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.423 KB) | DOI: 10.4308/hjb.15.2.71

Abstract

Penicillin G acylase (PGA) which catalyses penicillin G hydrolysis reaction is a key enzyme for the industrial production of penicilin G derivatives used in therapeutics. A new local strain of Bacillus subtilis BAC4 was found capable of producing extracellular PGA. However, characteristics of this extracellular PGA are not known. The goal of this research was to characterize the extracellular PGA produced by B. subtilis BAC4. Enzyme production was carried out by batch fermentation, followed by enzyme purification and characterization of the PGA. The PGA activity was determined by the Kornfeld method, with optimal activity for hydrolysing penicillin G observed at 43 oC and pH 8.5. The activation energy of penicillin G hydrolysis by the PGA of B. subtilis BAC4 was determined as 4.9 kcal.mol-1 and Vmax and Km values were found to be 0.7 µmole.min-1.mg-1 and 3.5 mM respectively. PGA catalytic activity was competitively inhibited by phenylacetic acid with an inhibition constant, Ki(PAA) , of 347.2 mM. It was concluded that the extracellular PGA of B. subtilis BAC4 can hydrolyse penicillin G efficiently. Key words: PGA, extracellular, Bacillus, local
Improving the Effectiveness of Crude-Oil Hydrocarbon Biodegradation Employing Azotobacter chroococcum as Co-Inoculant PUJAWATI SURYATMANA PARNADI; EDWAN KARDENA; ENNY RATNANINGSIH; . WISJNUPRAPTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.311 KB) | DOI: 10.5454/mi.1.1.2

Abstract

Azotobacter chroococcum has a great potential as biosurfactant producing bacteria and was used as co-inoculant to promote the rate of hydrocarbon biodegradation. The rate of hydrocarbon biodegradation were 0.01212, 0.01582, and 0.01766 per day for Acinetobacter sp., Bacillus cereus and the consorsium culture respectively. On the other hand, the rates of biodegradation using Azotobacter as co-inoculant were 0.1472, 0.01612, and 0.02709 g per day. Azotobacter chroococcum co-inoculant has the capability of increasing biodegradation efficiency of crude oilhydrocarbon. The biodegradation efficiency of petroleum hidrocarbon was increated by 13.4, 14.6, and 14.4% within the Petrobacter cultures.
Identifikasi Senyawa Alkaloid dari Akar Piper sarmentosum Roxb. Ex Hunter dan Uji Aktivitasnya terhadap Jamur Candida albicans Hartiwi Diastuti; Sadiah Achmad; Enny Ratnaningsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 2 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.2.96

Abstract

Piper sarmentosum Roxb. Ex Hunter or “Sirih duduk” has long been used for traditional medicine to cure various diseases, such as fungus infections. The investigation of the bioactive compounds of P. sarmentosum roots has not been carried out. This research was aimed to isolate the bioactive compounds from P. sarmentosum roots. The results showed that methanol extracts of P. sarmentosum roots have an activity on Candida albicans. The separation a bioactive compounds from methanol extracts of P. sarmentosum roots was performed by column chromatography, thin layer chromatography and recrystalizations. The identifications of the bioactive compounds were carried out using ultra violet spectrometry, infrared spectrometry, gas chromatography-mass spectrometry and nuclear magnetic resonance spectrometry. The results indicated that from methanol extracts, an alkaloid compound of piperoylpyrol derivative was 5-hydroxy-5- (3,4-methylenedioxyphenyl)-2-pentenoyl pyrol, could be purely isolated. Examination of bioactivity at concentration 0.10-2.50 mg/ml showed that this compound had an activity on C. albicans.
Co-Authors . WISJNUPRAPTO DEBBIE SOEFIE RETNONINGRUM Debbie Sofie Retnoningrum EDWAN KARDENA Endang Purwantini Hartiwi Diastuti Muhaimin Muhaimin OEI BAN LIANG Oei Ban Liang Pujawati Suryatmana Sadiah Achmad Sadijah Achmad Supartono -