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All Journal Partner JURNAL KAJIAN VETERINER
Eni Rohyati, Eni
Politeknik Pertanian Negeri Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Gambaran Mikroskopis Nekrosa Sel dan Deplesi Folikel Limfoid Bursa Fabricius Ayam Broiler Pasca Pemberian Probiotik B-mix dan Infeksi Salmonella enteritidis Eni Rohyati
Partner Vol 19, No 1 (2012): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v19i1.125

Abstract

Profile of Microscopic Necrosis Cells and Depilation of Lymphoid Follicle of Bursa of Broiler after Probiotic B Mix Administration and Enteritic salmonella Infection. Research to determine the incidence of necrosis cells and depletion of lymphoid follicles of bursa of fabricius after administration of B-mix probiotic and infection of Salmonella enteritidis (SE) was performed on 16 of broiler chickens strain abor acress divided into 4 groups; control group (IA), infected by SE (IB), Probiotics without SE infection (2A) and probiotic with infected SE (2B). Probiotic was daily administered at a dose 2 ml/litre of drinking water start at DOC (day old chick) to 6 weeks old chicken. Infection performed at the 4 weeks old chicken with dose 108 CFU/ml of drinking water. Observation and statistical tests has indicate that the groups were administration with B-mix probiotic could be increase the proliferation of lymphocyte cell so that cells of lymphoid follicles is more dense than in the control group, while administration of B-mix probiotic in chickens had been infected by SE, can reduce the incidence of depletion of lymphoid follicles and nekrosa cells. Key words: Bacillus, probiotic, Salmonella enteritidis, bursa fabricius, depletion
UJI TAPIS BRUSELLOSIS PADA SAPI DI RPH OEBA KOTA KUPANG DENGAN MENGGUNAKAN UJI RBT Eni Rohyati; Noviantie N Toelle; Erda Rame Hau
Partner Vol 23, No 2 (2018): Edisi November
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v23i2.313

Abstract

The aim of this research is detecting Brucella abortus reactor in Rumah Potong Hewan (RPH) Oeba, Kupang City  has been carried out using RBT serological test with RBT kits produced by Pusvetma. 105 serum samples were collected randomly from cattle in RPH Oeba in 2018, with details of 7samples from bulls and 98 samples from female cattle, and have been used for this study. All samples were collected with additional data of gender. The results showed that 60 samples were positive and 45 gave negative results.  Serum samples that are positive for RBT mostly come from female cattles which are 56 and only 4 bulls gave positive results. The average prevalence is 57%, the majority of the RBT test results from positive samples on the RBT test is +1 scoring (the lowest score in the RBT test).
KAJIAN KELAYAKAN OPERASIONAL RUMAH PEMOTONGAN HEWAN (RPH) OEBA PEMERINTAH KOTA KUPANG NUSA TENGGARA TIMUR DALAM MENGHASILKAN DAGING DENGAN KUALITAS ASUH Eni Rohyati; Bernadus Ndoen; Cardial L.O. Leo-Penu
Partner Vol 17, No 2 (2010): Edisi November
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v17i2.62

Abstract

Based on the result of Rohyati,et al (2007), the RPH Oeba did not fulfill 82,78% of complex and operational requirements or only meet 19.25% to those standards  in producing meat with ASUH quality (Safe, hygiene, Wholesome, and Halal). Through this article, the writer tried to analyze about the appropriate level of operational activity of RPH Oeba in meat producing with ASUH quality. Some of operational in the slaughterhouse that not fulfilled those standards are: No attention for workers hygiene and animal welfare, all operational using of non-hygiene water, using nonstandardized equipments,  not appropriate based on Islamic rules, skinning and carcass handling were not done properly, slaughtering process was not following the direction of stream production, “Clean and dirty” operational activities were located in the same areas, ante mortem and postmortem inspection were not done, sick animal and productive female were also slaughtered, sick animal was not isolated in quarantine pensKeywords: slouhterhouse, Oeba, meat quality
AKTIVITAS ANTIBAKTERI NIRA LONTAR TERFERMENTESI DENGAN VARIASI LAMA WAKTU FERMENTASI TERHADAP BAKTERI GRAM POSITIF (Staphylococcus aureus) DAN GRAM NEGATIF (Escherichia coli) Erda Eni Rame Hau; Eni Rohyati
JURNAL KAJIAN VETERINER Vol 5 No 2 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i2.957

Abstract

This study aims to determine the effect of spontaneous fermented nira with different treatment time to gram positive and gram negative bacteria. The research was conducted in November 2017 at the Animal Health Laboratory, Kupang State Agricultural of Polytechnic and Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Nusa Cendana University, Kupang, NTT. Samples of bacteria used in this study were Staphylococcus aureus and Escherichia coli bacteria. The fermented of nira lontar from 4 fermentation time (1 day (N1), 2 days (N2), 3 days (N3), and 4 days (N4)) were then collected to test the antimicrobial activity in inhibiting the growth of the microorganism. Fermentation was carried out for 4 days because previous research showed that the optimum growth of lactic acid bacteria in fermented palm was on the 4th day. This study used the Kirby-Bauer disc method, in which antibacterial activity was described by the diameter of the inhibition zone. The results showed that the mean diameter of the inhibitory zone of S. aureus with N1 material was 8.15 mm, while N2, N3, and N4 had the same diameter of 6.4 mm. There was a significant difference between the diameter of the N1 zone and the rest of the samples, as well as all treatments with Ciprofloxacin positive control antibiotics. Different trends were indicated by E. coli, where there were no significant differences between N1, N2, and N3 diameters with values of 9.2 mm, 8.95 mm, and 8.1 mm, respectively, but they were significantly different from the diameter N4 of 6.4mm, and all treatments were significantly different with Ciprofloxacin positive control antibiotics. It can be concluded that spontaneous fermented nira lontar at four different fermentation time intervals have antimicrobial activity against gram-positive and gram-negative bacteria, with statistically significantly different inhibitory activity among different fermentation days. Moreover, fermented nira lontar has higher inhibitory activity on gram-negative bacteria, E. coli compared to gram-positive bacteria, S. aureus.
KARAKTERISTIK DAN PENGGUNAAN LIPOPOLISAKARIDA (LPS) BRUCELLA DALAM MEDIK VETERINER Eni Rohyati; Erda E. Rame Hau; Noviani N. Toelle; Gerson Y. I. Sakan
Partner Vol 27, No 2 (2022): Edisi November 2022
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v27i2.1058

Abstract

Lipopolysaccharide (LPS) is a major component of cell membranes with gram negative bacteria including Brucella. The stain of B.abortus, B.melitensis, and B.suiscan be rough or smooth strains and express rough LPS (R-LPS)) or smooth LPS (S-LPS) as the main surface antigen; while, B .ovis and B.canis are two natural rough species that express R-LPS as the main antigen. R-LPS in B.abortus RB51 which is a stable R-LPS mutated from 2308 virulent S strains, while S-LPS is produced from B.abortus S1119-3 or S99 which is extracted by heating and then cooled. Both types of LPS can stimulate the humoral and innate immunity host immunity response. Those types are used as antigens in the diagnostic device and as immunogens of vaccines.  Keyword; LPS bakteri, Brucella, Medik Veteriner
Co-Authors Bernadus Ndoen Cardial L.O. Leo-Penu, Cardial L.O. Erda E. Rame Hau Erda Eni Rame Hau Erda Rame Hau Gerson Y. I. Sakan Noviani N. Toelle Novianti Neliyani Toelle